Abstract
Mammalian cells encode a diverse set of Rab GTPases and their corresponding regulators. In vitro biochemical screening has proven invaluable in assigning particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specificity in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or conditions that might change observed in vitro specificity.
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Acknowledgement
This research was supported by a grant from the National Institutes of Health (NIDDK 37332-28) to SRP.
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Nottingham, R.M., Pfeffer, S.R. (2015). Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts. In: Li, G. (eds) Rab GTPases. Methods in Molecular Biology, vol 1298. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2569-8_5
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DOI: https://doi.org/10.1007/978-1-4939-2569-8_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2568-1
Online ISBN: 978-1-4939-2569-8
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