Abstract
The ability to culture different cell types is essential for answering many questions in developmental and regenerative biology. Studies in marine organisms, in particular echinoderms, have been limited by the lack of well-described cellular culture systems. Here we describe a cell culture system, for normal or regenerating holothurian cells, that allows cell characterization by immunohistochemistry and scanning electron microscopy. These cell cultures can now be used to perform multiple types of experiments in order to explore the cellular, biochemical, and genomic aspects of echinoderm regenerative properties.
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Acknowledgements
This project was supported by NIH (Grant 1SC1GM084770-01, 1R03NS065275-01), NSF (IOS-0842870), and the University of Puerto Rico. We would like to thank to the Material Characterization Center of the University of Puerto Rico for the use of the Scanning Electron Microscopy Facility. We gratefully acknowledge Dr. Cristiano Di Benedetto (University of Milan) for his help concerning the scanning electron microscopy protocol.
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Bello, S.A., Abreu-Irizarry, R.J., García-Arrarás, J.E. (2015). Primary Cell Cultures of Regenerating Holothurian Tissues. In: Nelson, C. (eds) Tissue Morphogenesis. Methods in Molecular Biology, vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_19
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DOI: https://doi.org/10.1007/978-1-4939-1164-6_19
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Publisher Name: Humana Press, New York, NY
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