Abstract
Gene targeting is a highly effective and straightforward technique for the functional analysis of a gene of interest. However, its efficiency is not satisfactorily high in many model plants including Arabidopsis thaliana. In the moss Physcomitrella patens, a model species of basal plants, the efficiency of gene targeting is as high as in yeasts, and this moss is becoming widely recognized as an experimental model of choice in various areas of plant biology. Here we focus on the transformation of protoplast cells and on the measurement of bioluminescence rhythms from protonema tissues of luciferase reporter strains in P. patens, both of which are important for mechanistic studies of the circadian clock.
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Acknowledgement
We thank Mitsuyasu Hasebe and members of his laboratory (National Institute for Basic Biology, Okazaki) for kindly sharing the PHYSCOmanual protocol and various materials throughout our study using P. patens. We also thank Jean-Pierre Zrÿd (University of Lausanne) for kindly sharing a plasmid containing the 213 locus and Kyowa Hakko for kindly sharing Driselase. This work was supported by grants (21570005 and 24570007) from the Japan Society for the Promotion of Science to SA.
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Aoki, S., Okada, R., Satbhai, S.B. (2014). Transformation and Measurement of Bioluminescence Rhythms in the Moss Physcomitrella patens . In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_22
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DOI: https://doi.org/10.1007/978-1-4939-0700-7_22
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