Abstract
Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.
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Acknowledgement
This work was supported by US Friends of Hebrew University grant 0367445, ISF grant 0398636 and DFG grant 0308300.
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Shor, E., Hassidim, M., Green, R.M. (2014). The Use of Fluorescent Proteins to Analyze Circadian Rhythms. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_14
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DOI: https://doi.org/10.1007/978-1-4939-0700-7_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0699-4
Online ISBN: 978-1-4939-0700-7
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