Abstract
Rapid and accurate cancer drug screening is of great importance in precision medicine. However, the limited amount of tumor biopsy samples has hindered the application of traditional drug screening methods with microwell plates for individual patients. A microfluidic system provides an ideal platform for handling trace amounts of samples. This emerging platform has a good role in nucleic acid-related and cell related assays. Nevertheless, convenient drug dispensing remains a challenge for clinical on-chip cancer drug screening. Similar sized droplets are merged to add drugs for a desired screened concentration which significantly complicated the on-chip drug dispensing protocols. Here, we introduce a novel digital microfluidic system with a specially structured electrode (a drug dispenser) to dispense drugs by droplet electro-ejection under a high-voltage actuation signal, which can be conveniently adjusted by external electric controls. With this system, the screened drug concentrations span up to four orders of magnitude with small sample consumption. Various amounts of drugs can be delivered to the cell sample with desired amount in a flexible electric control. Moreover, single drug or combinatorial multidrug on-chip screening can be readily achieved. The drug response of normal MCF-10A breast cells and MDA-MB-231 breast tumor cells to two chemotherapeutic substances, cisplatin (Cis) and epirubicin (EP), was tested individually and in combination for proof-of-principle verification. The comparable on-chip and off-chip results confirmed the feasibility of our innovative DMF system for cancer drug screening.
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References
Du G-S, Pan J-Z, Zhao S-P, Zhu Y, den Toonder JMJ, Fang Q (2013) Anal Chem 85:6740–6747
Li J, Tan W, Xiao W, Carney RP, Men Y, Li Y, Quon G, Ajena Y, Lam KS, Pan T (2018) Anal Chem 90:13969–13977
Zhai J, Yi S, Jia Y, Mak P-I, Martins RP (2019) TrAC Trends Anal Chem 117:231–241
Wlodkowic D, Cooper JM (2010) Curr Opin Chem Biol 14:556–567
Eduati F, Utharala R, Madhavan D, Neumann UP, Longerich T, Cramer T, Saez-Rodriguez J, Merten CA (2018) Nat Commun 9:2434
Rane TD, Zec HC, Wang T-H (2015) Anal Chem 87:1950–1956
Theberge AB, Mayot E, El Harrak A, Kleinschmidt F, Huck WTS, Griffiths AD (2012) Lab Chip 12:1320
Shah GJ, Ding H, Sadeghi S, Chen S, Kim CJ, van Dam RM (2013) Lab Chip 13:2785
Gong J, C.-J. (2008) “CJ” Kim. Lab Chip 8:898
Zhai J, Li C, Li H, Yi S, Yang N, Miao K, Deng C, Jia Y, Mak P-I, Martins RP (2021) Lab Chip 21:4749–4759
Braut-Boucher F, Pichon J, Rat P, Adolphe M, Aubery M, Font J (1995) J Immunol Methods 178:41–51
Acknowledgments
This work was supported by the Macau Science and Technology Development Fund (FDCT) (FDCT0072/2020/AGJ, FDCT0029/2021/A1, SKL-AMSVIJUM/2020-2022), the University of Macau (MYRG2018-00114-AMSV), the Zhuhai Science and Technology Innovation Bureau (ZH22036302200052PWC, ZH22017002200012PWC), DAAN Gene Co., Ltd. (EF009/AMSV JYW/2018/GSTIC), and the Guangdong Department of Science and Technology (2021A0505080002). We would like to thank the technical and administrative team of the State Key Laboratory of Analog- and Mixed-Signal VLSI at the University of Macau for all their support.
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Li, C., Zhai, J., Jia, Y. (2023). Digital Microfluidics with an On-Chip Drug Dispenser for Single or Combinational Drug Screening. In: Garcia-Cordero, J.L., Revzin, A. (eds) Microfluidic Systems for Cancer Diagnosis . Methods in Molecular Biology, vol 2679. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3271-0_3
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DOI: https://doi.org/10.1007/978-1-0716-3271-0_3
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