Abstract
Circulating tumor cells (CTCs) are an important liquid biopsy biomarker for next-generation cancer diagnosis and prognosis. However, their clinical usage is hindered by the rarity of CTCs in patient’s peripheral blood. Microfluidics has shown unique advantages in CTC isolation and detection. We have developed lateral filter array microfluidic (LFAM) devices for highly efficient CTC isolation. In this chapter, we describe in detail the design and fabrication of the LFAM devices and their applications for CTC enumeration from clinical blood samples.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Klein CA (2008) The metastasis cascade. Science 321(5897):1785–1787
Poudineh M, Sargent EH, Pantel K, Kelley SO (2018) Profiling circulating tumour cells and other biomarkers of invasive cancers. Nat Biomed Eng 2(2):72–84. https://doi.org/10.1038/s41551-018-0190-5
Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, Reuben JM, Doyle GV, Allard WJ, Terstappen LW (2004) Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med 351(8):781–791
Paterlini-Brechot P, Benali NL (2007) Circulating tumor cells (CTC) detection: clinical impact and future directions. Cancer Lett 253(2):180–204
Danila DC, Heller G, Gignac GA, Gonzalez-Espinoza R, Anand A, Tanaka E, Lilja H, Schwartz L, Larson S, Fleisher M, Scher HI (2007) Circulating tumor cell number and prognosis in progressive castration-resistant prostate cancer. Clin Cancer Res 13(23):7053–7058. https://doi.org/10.1158/1078-0432.ccr-07-1506
Chen K, Amontree J, Varillas J, Zhang J, George TJ, Fan ZH (2020) Incorporation of lateral microfiltration with immunoaffinity for enhancing the capture efficiency of rare cells. Sci Rep 10(1):1–12. https://doi.org/10.1038/s41598-020-71041-7
Varillas JI, Zhang J, Chen K, Barnes II, Liu C, George TJ, Fan ZH (2019) Microfluidic isolation of circulating tumor cells and cancer stem-like cells from patients with pancreatic ductal adenocarcinoma. Theranostics 9(5):1417–1425. https://doi.org/10.7150/thno.28745
Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M (2007) Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature 450(7173):1235–1239
Stott SL, Hsu C-H, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M (2010) Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci 107(43):18392–18397. https://doi.org/10.1073/pnas.1012539107
Mohamadi RM, Besant JD, Mepham A, Green B, Mahmoudian L, Gibbs T, Ivanov I, Malvea A, Stojcic J, Allan AL, Lowes LE, Sargent EH, Nam RK, Kelley SO (2015) Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations. Angew Chem Int Ed Engl 54(1):139–143. https://doi.org/10.1002/anie.201409376
Vona G, Sabile A, Louha M, Sitruk V, Romana S, Schütze K, Capron F, Franco D, Pazzagli M, Vekemans M, Lacour B, Bréchot C, Paterlini-Bréchot P (2000) Isolation by size of epithelial tumor cells: a new method for the Immunomorphological and molecular characterization of circulating tumor cells. Am J Pathol 156(1):57–63
Sollier E, Go DE, Che J, Gossett DR, O'Byrne S, Weaver WM, Kummer N, Rettig M, Goldman J, Nickols N, McCloskey S, Kulkarni RP, Di Carlo D (2014) Size-selective collection of circulating tumor cells using vortex technology. Lab Chip 14(1):63–77. https://doi.org/10.1039/c3lc50689d
McFaul SM, Lin BK, Ma H (2012) Cell separation based on size and deformability using microfluidic funnel ratchets. Lab Chip 12(13):2369–2376
Park ES, Jin C, Guo Q, Ang RR, Duffy SP, Matthews K, Azad A, Abdi H, Todenhofer T, Bazov J, Chi KN, Black PC, Ma H (2016) Continuous flow deformability-based separation of circulating tumor cells using microfluidic ratchets. Small 12(14):1909–1919. https://doi.org/10.1002/smll.201503639
Moon H-S, Kwon K, Kim S-I, Han H, Sohn J, Lee S, Jung H-I (2011) Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP). Lab Chip 11(6):1118–1125
Ding X, Peng Z, Lin S-CS, Geri M, Li S, Li P, Chen Y, Dao M, Suresh S, Huang TJ (2014) Cell separation using tilted-angle standing surface acoustic waves. Proc Natl Acad Sci U S A 111(36):12992–12997. https://doi.org/10.1073/pnas.1413325111
Chen K, Dopico P, Varillas J, Zhang J, George TJ, Fan ZH (2019) Integration of lateral filter arrays with Immunoaffinity for circulating-tumor-cell isolation. Angew Chem Int Ed Engl 58(23):7606–7610. https://doi.org/10.1002/anie.201901412
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Chen, K., George, T.J., Fan, Z.H. (2023). Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells. In: Garcia-Cordero, J.L., Revzin, A. (eds) Microfluidic Systems for Cancer Diagnosis . Methods in Molecular Biology, vol 2679. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3271-0_1
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3271-0_1
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3270-3
Online ISBN: 978-1-0716-3271-0
eBook Packages: Springer Protocols