Abstract
Gangliosides play a variety of physiological roles and are one of the most important lipid raft constituents. However, their dynamic behaviors have scarcely been investigated in living cells because of the lack of fluorescent probes that behave like their parental molecules. Recently, fluorescent ganglioside probes that mimic native ganglioside behaviors have been developed. In this chapter, I discuss the recent advances in research related to the lateral localization and dynamic behaviors of gangliosides in the plasma membranes of living cells.
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Acknowledgments
This work was supported in part by Grants-in-Aid for Specific Research Kiban B (21H02424) and for Innovative Areas (20 K21387) from the Japan Society for the Promotion of Science, by a grant from Core Research for Evolutional Science and Technology (CREST project of “Elucidation of Biological Mechanism of Extracellular Fine Particles and the Control System”) of the Japan Science and Technology Agency (JPMJCR18H2), and by a grant from the Takeda Foundation. WPI-iCeMS of Kyoto University is supported by the World Premiere Research Center Initiative (WPI) of the ΜEXT.
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Suzuki, K.G.N. (2023). Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes. In: Kabayama, K., Inokuchi, Ji. (eds) Glycolipids. Methods in Molecular Biology, vol 2613. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2910-9_17
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DOI: https://doi.org/10.1007/978-1-0716-2910-9_17
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