Abstract
Traditionally, capillary electrophoresis (CE) has been ruled out of many food safety applications, despite its inherent advantages, because its concentration sensitivity has been not high enough, mainly in relation to the monitoring of contaminants and residues, such as pesticides, veterinary medicines, environmental contaminants, toxins, etc. For this reason, researchers have proposed several strategies to overcome this limitation. So far, approaches based on chromatographic principles have been the most successful solutions. These approaches, known as in-line solid phase extraction, consist of the introduction of a small amount of stationary phase in the inlet section of the electrophoretic capillary (analyte concentrator, AC) to retain the analytes before separation takes place. In this chapter, this strategy is applied to CE coupled to tandem mass spectrometry (MS/MS) for the multiresidue detection of quinolone antibiotic residues in chicken meat. A previous sample treatment based on pressurized liquid extraction to obtain an optimum performance is also described.
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References
Hernández-Mesa M, Lara FJ, Moreno-González D, del Olmo-Iruela M, García-Campaña AM (2020) Trends in multiresidue analysis. In: Meyers RA (ed) Encyclopedia of analytical chemistry. Wiley, Hoboken
Regulation (EC) No 396/2005 of the European Parliament and the Council of 23 February 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending Council Directive 91/414/EEC. J Eur Com L70:1–16
(2010) Commission Regulation (EU) No 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. J Eur Union L15:1–72
Thormann W (2018) Theoretical principles of capillary electromigration methods. In: Poole CF (ed) Handbooks in separation science. Elsevier, Amsterdam
Lara FJ, Moreno-González D, Hernández-Mesa M, García-Campaña AM (2018) Food safety applications of capillary electromigration methods. In: Poole CF (ed) Handbooks in separation science. Elsevier, Amsterdam
Hernández-Mesa M, Moreno-González D, Lara FJ, García-Campaña AM (2019) Capillary electrophoresis | Food chemistry applications. In: Reedijk J (ed) Encyclopedia of analytical science, 3rd edn. Elsevier, Amsterdam
Breadmore MC, Grochocki W, Kalsoom U et al (2019) Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018). Electrophoresis 40:17–39
Breadmore MC, Wuethrich A, Li F et al (2016) Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016). Electrophoresis 38:33–59
Šlampová A, Malá Z, Gebauer P (2019) Recent progress of sample stacking in capillary electrophoresis (2016–2018). Electrophoresis 40:40–54
Pont L, Pero-Gascon R, Gimenez E, Sanz-Nebot V, Benavente F (2019) A critical retrospective and prospective review of designs and materials in in-line solid-phase extraction capillary electrophoresis. Anal Chim Acta 1079:1–19
Ramautar R, Somsen GW, de Jong GJ (2016) Developments in coupled solid-phase extraction-capillary electrophoresis 2013-2015. Electrophoresis 37:35–44
Ramautar R, Somsen GW, de Jong GJ (2014) Developments in coupled solid-phase extraction-capillary electrophoresis 2011-2013. Electrophoresis 35:128–137
Ramautar R, de Jong GJ, Somsen GW (2011) Developments in coupled solid‐phase extraction–capillary electrophoresis 2009–2011. Electrophoresis 33:243–250
Ramautar R, Somsen GW, de Jong GJ (2009) Recent developments in coupled SPE‐CE. Electrophoresis 31:44–54
Puig P, Borrull F, Calull M, Aguilar C (2007) Recent advances in coupling solid-phase extraction and capillary electrophoresis (SPE–CE). TrAC Trends Anal Chem 26:664–678
Benavente F, Medina-Casanellas S, Giménez E, Sanz-Nebot V (2016) On-line solid-phase extraction capillary electrophoresis mass spectrometry for preconcentration and clean-up of peptides and proteins. In: Tran NT, Taverna M (eds) Capillary electrophoresis of proteins and peptides. Methods in molecular biology, vol 1466. Humana Press, New York, pp 67–84
Pero-Gascon R, Pont L, Sanz-Nebot V, Benavente F (2019) On-line immunoaffinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of serum transthyretin. In: Phillips T (ed) Clinical applications of capillary electrophoresis. Methods in molecular biology, vol 1972. Humana Press, New York, pp 57–76
Jooß K, Sommer J, Bunz SC, Neusüß C (2013) In-line SPE-CE using a fritless bead string design—application for the analysis of organic sulfonates including inline SPE-CE-MS for APTS-labeled glycans. Electrophoresis 35:1236–1243
Wang S, Xie T, Dong X et al (2019) Determination of iodine species in seafood by ionic liquid-base in-line solid-phase extraction-capillary electrophoresis. Food Anal Methods 12:2139–2149
Moreno-González D, Jáč P, Riasová P, Nováková L (2021) In-line molecularly imprinted polymer solid phase extraction-capillary electrophoresis coupled with tandem mass spectrometry for the determination of patulin in apple-based food. Food Chem 334:127607
Moreno-González D, Lara FJ, Gámiz-Gracia L, García-Campaña AM (2014) Molecularly imprinted polymer as in-line concentrator in capillary electrophoresis coupled with mass spectrometry for the determination of quinolones in bovine milk samples. J Chromatogr A 1360:1–8
Lara FJ, García-Campaña AM, Alés-Barrero F, Bosque-Sendra JM (2008) In-line solid-phase extraction preconcentration in capillary electrophoresis-tandem mass spectrometry for the multiresidue detection of quinolones in meat by pressurized liquid extraction. Electrophoresis 29:2117–2125
Marechal A, Jarrosson F, Randon J, Dugas V, Demesmay C (2015) In-line coupling of an aptamer based miniaturized monolithic affinity preconcentration unit with capillary electrophoresis and laser induced fluorescence detection. J Chromatogr A 1406:109–117
Commission Decision 2002/657/EEC implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. J Eur Commun L221:8–36
Acknowledgments
This work was supported by MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa” (Project No. RTI2018-097043-B-I00) and Spanish Network of Excellence in Sample preparation (RED2018-102522-T). Thanks to Junta de Andalucía-Programa Operativo FEDER (Project No. B-AGR-202-UGR20).
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Lara, F.J., García-Campaña, A.M. (2022). Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction Coupled to Capillary Electrophoresis–Tandem Mass Spectrometry. In: Neusüß, C., Jooß, K. (eds) Capillary Electrophoresis-Mass Spectrometry . Methods in Molecular Biology, vol 2531. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2493-7_15
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DOI: https://doi.org/10.1007/978-1-0716-2493-7_15
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