Abstract
Approximately one in three people infected with the SARS-CoV-2 virus have mild symptoms or are asymptomatic. However, these individuals can still spread the virus. Regular self-testing can help to detect these individuals and thereby slow the spread and protect the more vulnerable members of society. Here, we present a protocol for use of the COVID-19 rapid antigen test which was made freely available to residents of the United Kingdom in April of this year. This using the lateral flow technique for detection of antigens and is amenable to multiplexing.
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References
Manigandan S, Wu MT, Ponnusamy VK et al (2020) A systematic review on recent trends in transmission, diagnosis, prevention and imaging features of COVID-19. Process Biochem 98:233–240
Hussein M, Toraih E, Elshazli R et al (2020) Meta-analysis on serial intervals and reproductive rates for SARS-CoV-2. Ann Surg 273(3):416–423
Salian VS, Wright JA, Vedell PT et al (2021) COVID-19 transmission, current treatment, and future therapeutic strategies. Mol Pharm 18(3):754–771
Gómez CE, Perdiguero B, Esteban M (2021) Emerging SARS-CoV-2 variants and impact in global vaccination programs against SARS-CoV-2/COVID-19. Vaccines (Basel) 9(3):243. https://doi.org/10.3390/vaccines9030243
Peacock TP, Penrice-Randal R, Hiscox JA et al (2021) SARS-CoV-2 one year on: evidence for ongoing viral adaptation. J Gen Virol 102(4). https://doi.org/10.1099/jgv.0.001584
Salzberger B, Buder F, Lampl B et al (2021) SARS-CoV-2/COVID-19-epidemiology and prevention. Nephrologe 16:1–7. https://doi.org/10.1007/s11560-020-00472-0. Online ahead of print
Brosnahan SB, Jonkman AH, Kugler MC et al (2020) COVID-19 and respiratory system disorders: current knowledge, future clinical and translational research questions. Arterioscler Thromb Vasc Biol 40(11):2586–2597
Castro RA, Frishman WH (2021) Thrombotic complications of COVID-19 infection: a review. Cardiol Rev 29(1):43–47
Li X, Wang L, Yan S et al (2020) Clinical characteristics of 25 death cases with COVID-19: a retrospective review of medical records in a single medical center, Wuhan, China. Int J Infect Dis 94:128–132
Qiu P, Zhou Y, Wang F et al (2020) Clinical characteristics, laboratory outcome characteristics, comorbidities, and complications of related COVID-19 deceased: a systematic review and meta-analysis. Aging Clin Exp Res 32(9):1869–1878
Rashedi J, Mahdavi Poor B, Asgharzadeh V et al (2020) Risk factors for COVID-19. Infez Med 28(4):469–474
Mahalmani VM, Mahendru D, Semwal A et al (2020) COVID-19 pandemic: a review based on current evidence. Indian J Pharmacol 52(2):117–129
Esakandari H, Nabi-Afjadi M, Fakkari-Afjadi J et al (2020) A comprehensive review of COVID-19 characteristics. Biol Proced Online 22:19. https://doi.org/10.1186/s12575-020-00128-2
Augustine R, Das S, Hasan A et al (2020) Rapid antibody-based COVID-19 mass surveillance: relevance, challenges, and prospects in a pandemic and post-pandemic world. J Clin Med 9(10):3372. https://doi.org/10.3390/jcm9103372
Malik YA (2020) Properties of Coronavirus and SARS-CoV-2. Malays J Pathol 42(1):3–11
Satarker S, Nampoothiri M (2020) Structural proteins in severe acute respiratory syndrome Coronavirus-2. Arch Med Res 51(6):482–491
Rehman MFU, Fariha C, Anwar A et al (2021) Novel coronavirus disease (COVID-19) pandemic: a recent mini review. Comput Struct Biotechnol J 19:612–623
Cui F, Zhou HS (2020) Diagnostic methods and potential portable biosensors for coronavirus disease 2019. Biosens Bioelectron 165:112349. https://doi.org/10.1016/j.bios.2020.112349
Martín J, Tena N, Asuero AG (2021) Current state of diagnostic, screening and surveillance testing methods for COVID-19 from an analytical chemistry point of view. Microchem J 167:106305. https://doi.org/10.1016/j.microc.2021.106305
Shah J, Liu S, Potula HH et al (2021) IgG and IgM antibody formation to spike and nucleocapsid proteins in COVID-19 characterized by multiplex immunoblot assays. BMC Infect Dis 21(1):325. https://doi.org/10.1186/s12879-021-06031-9
https://www.gov.uk/government/news/new-campaign-urges-public-to-get-tested-twice-a-week
Yetisen AK, Akram MS, Lowe CR (2013) Paper-based microfluidic point-of-care diagnostic devices. Lab Chip 13(12):2210–2251
Koczula KM, Gallotta A (2016) Lateral flow assays. Essays Biochem 60(1):111–120
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Guest, P.C., Rahmoune, H. (2022). COVID-19 Detection Using the NHS Lateral Flow Test Kit. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 2511. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2395-4_22
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DOI: https://doi.org/10.1007/978-1-0716-2395-4_22
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2395-4
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