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Means to Quantify Vascular Cell File Numbers in Different Tissues

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Plant Cell Division

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2382))

Abstract

Oriented cell divisions are crucial throughout plant development to define the final size and shape of organs and tissues. As most of the tissues in mature roots and stems are derived from vascular tissues, studying cell proliferation in the vascular cell lineage is of great importance. Although perturbations of vascular development are often visible already at the whole plant macroscopic phenotype level, a more detailed characterization of the vascular anatomy, cellular organization, and differentiation status of specific vascular cell types can provide insights into which pathway or developmental program is affected. In particular, defects in the frequency or orientation of cell divisions can be reliably identified from the number of vascular cell files. Here, we provide a detailed description of the different clearing, staining, and imaging techniques that allow precise phenotypic analysis of vascular tissues in different organs of the model plant Arabidopsis thaliana throughout development, including the quantification of cell file numbers, differentiation status of vascular cell types, and expression of reporter genes.

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Acknowledgments

This work was funded by the European Research Council (ERC Starting Grant TORPEDO-714055); and an EMBO long-term fellowship (ALTF 1005-2019) to M.G.

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Authors and Affiliations

  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Helena E. Arents, Matouš Glanc & Bert De Rybel

  2. VIB Center for Plant Systems Biology, Ghent, Belgium

    Helena E. Arents, Matouš Glanc & Bert De Rybel

  3. Department of Biological and Environmental Sciences, Institute of Biotechnology, University of Helsinki, Helsinki, Finland

    Gugan Eswaran & Ari Pekka Mähönen

Authors
  1. Helena E. Arents
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  2. Gugan Eswaran
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  3. Matouš Glanc
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  4. Ari Pekka Mähönen
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  5. Bert De Rybel
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Corresponding author

Correspondence to Bert De Rybel .

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Editors and Affiliations

  1. SiCE, Laboratoire Reproduction et Développement des Plantes, ENS Lyon, Lyon, France

    Marie-Cécile Caillaud

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Arents, H.E., Eswaran, G., Glanc, M., Mähönen, A.P., De Rybel, B. (2022). Means to Quantify Vascular Cell File Numbers in Different Tissues. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 2382. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1744-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1744-1_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1743-4

  • Online ISBN: 978-1-0716-1744-1

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