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Plant Cell Division

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Marie-Cécile Caillaud

    1. SiCE, Laboratoire Reproduction et Développement des Plantes, ENS Lyon, Lyon, France

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2382)

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About this book

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Methods to Visualize the Actin Cytoskeleton During Plant Cell Division

    • Marie-Cécile Caillaud
    Pages 1-16

  3. Mitosis and Cell Cycle Progression

    1. Front Matter

      Pages 17-17
      Download chapter PDF

    2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

      • Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, Inna Lermontova
      Pages 19-28

    3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips

      • Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, William F. Thompson
      Pages 29-72

    4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures

      • Kateřina Bišová
      Pages 73-88

    5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission

      • Veronika Kselíková, Vilém Zachleder, Kateřina Bišová
      Pages 89-101

  4. Manipulation of Cell Division

    1. Front Matter

      Pages 103-103
      Download chapter PDF

    2. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy

      • Lukas Hoermayer, Jiří Friml, Matouš Glanc
      Pages 105-114

    3. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells

      • Tamara Jimenez-Gόngora, Huang Tan, Rosa Lozano-Durán
      Pages 115-127

  5. Quantification of Cell Division Patterning

    1. Front Matter

      Pages 129-129
      Download chapter PDF

    2. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf

      • Jingjing Fang, Xueyong Li
      Pages 131-140

    3. Studying Cell Division Plane Positioning in Early-Stage Embryos

      • Katia Belcram, Jean-Christophe Palauqui, Martine Pastuglia
      Pages 141-154

    4. Means to Quantify Vascular Cell File Numbers in Different Tissues

      • Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, Bert De Rybel
      Pages 155-179

    5. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis

      • Roya Campos, Jaimie M. Van Norman
      Pages 181-207

    6. Quantification of Cell Division Angles in the Arabidopsis Root

      • Katia Belcram, David Legland, Martine Pastuglia
      Pages 209-221

  6. Imaging and Quantifying Plant Cytokinesis

    1. Front Matter

      Pages 223-223
      Download chapter PDF

    2. A Quantitative Method for Evaluating Phragmoplast Morphology

      • Takema Sasaki, Yoshihisa Oda
      Pages 225-232

    3. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching During Cytokinesis

      • Alexandra Deli, Leda-Eleni Tympa, Panagiotis N. Moschou
      Pages 233-243

    4. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells

      • Keisho Maeda, Takumi Higaki
      Pages 245-252
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Editors and Affiliations

  • SiCE, Laboratoire Reproduction et Développement des Plantes, ENS Lyon, Lyon, France

    Marie-Cécile Caillaud

Bibliographic Information

  • Book Title: Plant Cell Division

  • Book Subtitle: Methods and Protocols

  • Editors: Marie-Cécile Caillaud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1744-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1743-4Published: 28 October 2021

  • Softcover ISBN: 978-1-0716-1746-5Published: 29 October 2022

  • eBook ISBN: 978-1-0716-1744-1Published: 27 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 267

  • Number of Illustrations: 18 b/w illustrations, 42 illustrations in colour

  • Topics: Plant Sciences, Cell Biology

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