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Methods to Study Posttranslational Modification Patterns in Cytotoxic T-Cells and Cancer

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Cytotoxic T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2325))

Abstract

Protein posttranslational modifications (PTMs) regulate intracellular signaling associated with development and progression of many diseases; thus, they are key to understanding pathological mechanisms and set up more tailored therapies. In addition, many posttranslationally modified proteins are released into biological fluids and can be used as new and more specific biomarkers. Based on this evidence, we analyzed the role of some PTMs in cancer and described the correlation between specific PTMs and T-cells activation/inhibition in cancer microenvironment. In the second part of this chapter, we analyzed the most commonly used approaches for qualitative and quantitative determination of PTMs. The comparison of three distinct but often complementary methodologies such as immunoblotting, mass spectrometry, and ELISA assays has allowed to highlight the pros and cons of each approach with a focus on their current application and their future developments to obtain more confident biomarkers and therapeutic targets useful for diagnosis, prognosis, and monitoring of the response to therapy.

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Authors and Affiliations

  1. R&D Unit, Fluidia srl, Foggia, Italy

    Annamaria Deleonardis

  2. Section of Nephrology, Dialysis and Transplantation, Department of Emergency and Organ Transplantation (DETO), University of Bari Aldo Moro, Bari, Italy

    Annamaria Deleonardis

  3. Clinical Pathology Unit, Department of Laboratory Diagnostics, Policlinic University Hospital “Riuniti”, Foggia, Italy

    Massimo Papale

Authors
  1. Annamaria Deleonardis
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  2. Massimo Papale
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Correspondence to Massimo Papale .

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Editors and Affiliations

  1. Department of Surgical and Medical Sciences, University of Foggia, Foggia, Italy

    Margherita Gigante

  2. Department of Surgical and Medical Sciences, University of Foggia, Foggia, Italy

    Elena Ranieri

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Deleonardis, A., Papale, M. (2021). Methods to Study Posttranslational Modification Patterns in Cytotoxic T-Cells and Cancer. In: Gigante, M., Ranieri, E. (eds) Cytotoxic T-Cells. Methods in Molecular Biology, vol 2325. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1507-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1507-2_10

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