Abstract
Carrot is a vegetable of increasing economic importance. New hybrid cultivars are constantly required to meet the changing market needs. The application of anther culture significantly shortens the difficult and long-lasting breeding of carrot. We examined all the stages of the process of generating androgenic plants: induction of embryos in anther cultures, regeneration and acclimatization of produced plants, their evaluation, ploidy and homozygosity, and many other factors affecting their effectiveness. Every factor has been optimized by experimentally selecting the optimal level. As a result, a full protocol of producing homozygous plants using anther cultures was developed, which is presented in this chapter.
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References
Seguí-Simarro JM, Nuez F (2008) How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore derived embryogenesis. Physiol Plant 134:1–12
Andersen SB, Christiansen I, Farestveit B (1990) Carrot (Daucus carota L.): In Vitro production of haploids and field trials. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, Vol. 12 haploids in crop improvement I. Springer-Verlag Berlin Heidelberg, Berlin, pp 393–402
Hu KL, Matsubara S, Murakami K (1993) Haploid plant production by anther culture in carrot (Daucus carota L.). J Jpn Soc Hortic Sci 2(3):561–565
Matsubara S, Dohya N, Murakami K, Nishio T, Dore C (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore culture. Acta Hortic 392:129–137
Tyukavin GB, Shmykova NA, Monakhova MA (1999) Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Physiol 46(6):767–773
Górecka K, Krzyżanowska D, Górecki R (2005) The influence of several factors on the efficiency of androgenesis in carrot. J Appl Genet 46(3):265–269
Bajaj YPS (1990) In vitro production of haploid and their use in cell genetic and plant breeding. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Vol. 12, haploid in crop improvement i. Springer Verlag, Berlin, pp 1–44
Özkum Çiner D, Tıpırdamaz R (2002) The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annum L.). Turk J Bot 26:131–139
Datta SK (2005) Androgenic haploids: factors controlling development and its application in crop improvement. Curr Sci 89(11):1870–1878
Bhojwani SS, Dantu PK (2010) Haploid plants. In: Davey MR, Anthony P (eds) Plant cell culture, essential methods. Wiley-Blackwell, Chichester, UK, pp 61–76
Arnison PG, Dodaldson P, Ho LC, Kellar WA (1990) The influence of various physiological parameters on anther culture of broccoli (Brassica oleracea var. italica). Plant Cell Tiss Org Culture 20:147–155
Roulund NL, Hansted L, Andersen SB, Farestveit B (1990) Effect of genotype, environment and carbohydrate on anther culture response in head cabbage (Brassica oleracea L. convar. capitata (L.) Alef.). Euphytica 49:237–242
Kiszczak W, Górecka K, Krzyżanowska D, Kowalska U (2005) Size of flower buds in carrot (Daucus carota l.) as an indicator of a stage of microsporogenesis and its suitability for induction of androgenesis. Veg Crops Res Bull 62:81–90
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50:148–151
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Kiszczak W, Kowalska U, Kapuścińska A, Burian M, Krystyna G (2015) Effect of low temperature on in vitro androgenesis of carrot (Daucus carota L.). In Vitro Cell Dev Biol-Plant 51:135–142
Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U (2009) Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Acta Physiol Plant 31:1139–1145
Tyukavin GB (2007) Carrot biotechnology. Dissertation, Russian Research Institute of Vegetable Breeding and Seed Production
Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U, Górecki R (2009) Carrot doubled haploids. In: Jain SM (ed) Touraev A. Forster BP, Advances in haploid production in higher plants. Springer Science, New York, pp 231–239
Kiszczak W, Krzyżanowska D, Strycharczuk K, Kowalska U, Wolko B, Górecka K (2011) Determination of ploidy and homozygosity of carrot plants obtained from anther cultures. Acta Physiol Plant 33(2):401–407
Westphal L, Wricke G (1989) Genetic analysis of DIA, GOT and PGI isozyme loci in Daucus carota L. ssp. sativas. Plant Breed 102:51–57
Górecka K, Krzyżanowska D, Kiszczak W, Górecki R (2005) Embryo induction in anther culture of Daucus carota L. Veg Crops Res Bull 63:25–32
Górecka K, Kiszczak W, Krzyżanowska D, Kowalska U, Kapuścińska A (2014) Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.). Turk J Biol 38:593–600
Galbraith DW (1984) Flow cytometric analysis of the cell cycle. Cell Cult Som Cell Genet Plants 1:765–777
Weeden FN, Gottlieb LD (1980) Isolation of cytoplasmic enzymes from pollen. Plant Phys 66:400–403
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Kiszczak, W., Burian, M., Kowalska, U., Górecka, K. (2021). Production of Homozygous Carrot (Daucus carota L.) Plants by Anther Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_7
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DOI: https://doi.org/10.1007/978-1-0716-1335-1_7
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