Anther culture of over 20 carrot cultivars was studied. The uni-nucleate stage proved optimal for embryogenesis for which bud length was used as a surrogate. Large genotypic variation in culture response was found. Anther culture at 27°C in the dark without sub-culturing had a significant beneficial impact on embryogenesis. Embryos were obtained regardless of donor plant culture; however, plants grown in glasshouse conditions produced more embryos than those from the field. Secondary embryogenesis was induced in some cultures and in one case, 102 plants were obtained from one embryo (on B5 medium without hormones) over a 12 week period. Regenerated plants were planted into peat and acclimatized to glasshouse and growth room conditions. Cytological and cytometric studies revealed that over 90% of regenerated plants possessed a doubled chromosome complement and isozyme analysis showed that 96–100% were homozygous. Anatomical studies confirmed that embryos had formed from microspores.
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Górecka, K., Krzyżanowska, D., Kiszczak, W., Kowalska, U., Górecki, R. (2009). Carrot Doubled Haploids. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_20
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