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Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cons of Different Techniques

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Regulatory B Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2270))

Abstract

Transforming growth factor (TGF)-β1 is one of the regulatory cytokines produced by B cells and has a pivotal role in intestinal homeostasis. TGF-β1 can determine the fate of naive T cells, such as differentiation, proliferation, and apoptosis, which are relevant to the pathogenesis of autoimmunity, infection, inflammation, allergy, and cancer. Here, we describe detailed methods for detection and quantification of TGF-β1 secreted by B cells using ELISA, flow cytometry, and real-time PCR.

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Author information

Authors and Affiliations

  1. Department of Internal Medicine II, Shimane University Faculty of Medicine, Izumo, Japan

    Yoshiyuki Mishima, Akihiko Oka & Shunji Ishihara

Authors
  1. Yoshiyuki Mishima
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  2. Akihiko Oka
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  3. Shunji Ishihara
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Corresponding author

Correspondence to Yoshiyuki Mishima .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Udine, Udine, Italy

    Francesca Mion

  2. Department of Medicine, University of Udine, Udine, Italy

    Silvia Tonon

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Mishima, Y., Oka, A., Ishihara, S. (2021). Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cons of Different Techniques. In: Mion, F., Tonon, S. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 2270. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1237-8_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1237-8_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1236-1

  • Online ISBN: 978-1-0716-1237-8

  • eBook Packages: Springer Protocols

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