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Hands on Native Mass Spectrometry Analysis of Multi-protein Complexes

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Multiprotein Complexes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2247))

Abstract

By maintaining intact multi-protein complexes in the gas-phase, native mass spectrometry provides their molecular weight with very good accuracy compared to other methods (typically native PAGE or SEC-MALS) (Marcoux and Robinson, Structure 21:1541–1550, 2013). Besides, heterogeneous samples, in terms of both oligomeric states and ligand-bound species can be fully characterized. Here we thoroughly describe the analysis of several oligomeric protein complexes ranging from a 16 = kDa dimer to a 801-kDa tetradecameric complex on different instrumental setups.

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Acknowledgments

The authors would like to thank Arnaud Poterszman (CAK complex), Albert Weixlbaumer (E. coli RNA polymerase), and Isabelle Billas Massobrio (ERR complexes) from the IGBMC (Strasbourg, France) and Virginie Gervais and Alain Milon (p8 protein) from IPBS (Toulouse, France) for kindly providing samples. This work was supported by the French Ministry of Research (Investissements d’Avenir Program, Proteomics French Infrastructure, ANR-10-INBS-08) and the FEDER (Fonds Européens de Développement Régional), Toulouse Métropole, and the Région Midi-Pyrénées.

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Authors and Affiliations

  1. Laboratoire de Spectroméérie de Masse BioOrganique, Universitéde Strasbourg, CNRS, IPHC UMR7178, 67000 Strasbourg, France

    Stéphane Erb

  2. Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubbert Curien (IPHC), Centre National de la Recherche Scientifique (CNRS), UMR 7178, Université de Strasbourg, Strasbourg, France

    Sarah Cianférani

  3. Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

    Julien Marcoux

Authors
  1. Stéphane Erb
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  2. Sarah Cianférani
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  3. Julien Marcoux
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Corresponding authors

Correspondence to Sarah Cianférani or Julien Marcoux .

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Editors and Affiliations

  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, Center for Integrated Biology, Integrated Structural Biology Department, Equipe labellisée Ligue Contre le Cancer, CNRS UMR 7104 - Inserm U 1258, University of Strasbourg, Illkirch, France

    Arnaud Poterszman

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Erb, S., Cianférani, S., Marcoux, J. (2021). Hands on Native Mass Spectrometry Analysis of Multi-protein Complexes. In: Poterszman, A. (eds) Multiprotein Complexes. Methods in Molecular Biology, vol 2247. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1126-5_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1126-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1125-8

  • Online ISBN: 978-1-0716-1126-5

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