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Native Protein Mass Spectrometry

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

In native mass spectrometry, non-covalent interactions are preserved in solution and through transfer to the gas phase. This technique can be used to characterize the composition, stoichiometry, and architecture of protein nano-assemblies, such as those observed in vivo or constructed through protein engineering in nanotechnology and synthetic biology. Here we describe an implementation of native mass spectrometry for studying protein-based nanostructures, including membrane proteins. Unambiguous structural details of assemblies can be rapidly determined due to the high resolution and mass accuracy afforded by mass spectrometry measurements including protein nano-assembly stoichiometry, heterogeneity, and ligand binding characteristics.

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Author information

Authors and Affiliations

  1. School of Physical and Chemical Sciences, University of Canterbury, Christchurch, New Zealand

    Timothy M. Allison

  2. Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK

    Mark T. Agasid

Authors
  1. Timothy M. Allison
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  2. Mark T. Agasid
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Corresponding author

Correspondence to Timothy M. Allison .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Allison, T.M., Agasid, M.T. (2020). Native Protein Mass Spectrometry. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

  • eBook Packages: Springer Protocols

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