Abstract
The subcellular localization of RNAs is regulated by cis-regulatory elements together with interacting trans factors. Here we describe a high-throughput sequencing-based method named REL-seq (RNA elements for subcellular localization by sequencing) to identify the cis-elements that contribute to RNA subcellular localization. By coupling REL-seq with random mutagenesis (mutREL-seq), we can further narrow down the cis-elements to key motifs at single-nucleotide resolution.
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Acknowledgments
This work is supported by the National Natural Science Foundation of China (20161310854, 31471219), the National Basic Research Program of China (2017YFA0504204, 2018YFA0107603), and the Center for Life Sciences at Tsinghua University. Y.Y. is supported by the Outstanding Postdoctoral Program of Tsinghua-Peking Joint Center for Life Sciences.
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Yin, Y., Shen, X. (2020). Identification of cis-Elements for RNA Subcellular Localization Through REL-seq. In: Ørom, U. (eds) RNA-Chromatin Interactions. Methods in Molecular Biology, vol 2161. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0680-3_11
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DOI: https://doi.org/10.1007/978-1-0716-0680-3_11
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