Abstract
The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
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Abbreviations
- GNA:
-
Galanthus nivalis agglutinin
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Acknowledgments
This work was supported mainly by grants from Ghent University and the Fund for Scientific Research-Flanders.
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Van Damme, E.J.M. (2020). Purification of GNA-Related Lectins from Natural Sources. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_40
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_40
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