Abstract
Stem cell tracking is an essential prerequisite for effective stem cell therapy. Computed tomography (CT) imaging technique is an emerging quantitative tool to detect real time distribution of transplanted cells. Most of CT labels based on the high atomic number (Z) materials have concern over biocompatibility. The present book chapter describes a protocol for the use of biocompatible gold nanoparticles as a CT marker for efficient labeling of mesenchymal stem cells (MSCs) and subsequent cell tracking in rodent models.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Nelson TJ, Behfar A, Yamada S, Martinez-Fernandez A, Terzic A (2009) Stem cell platforms for regenerative medicine. Clin Transl Sci 2(3):222–227
Bagno L, Hatzistergos KE, Balkan W, Hare JM (2018) Mesenchymal stem cell-based therapy for cardiovascular disease: progress and challenges. Mol Ther 26:7
Rossi DJ, Jamieson CH, Weissman IL (2008) Stems cells and the pathways to aging and cancer. Cell 132(4):681–696
Goldman S (2005) Stem and progenitor cell-based therapy of the human central nervous system. Nat Biotechnol 23(7):862–871
Kim J, Park JS (2017) Usage of human mesenchymal stem cells in cell-based therapy: advantages and disadvantages. Dev Reprod 21(1):1–10
Reagan MR, Seib FP, McMillin DW, Sage EK, Mitsiades CS, Janes SM et al (2012) Stem cell implants for cancer therapy: TRAIL-expressing mesenchymal stem cells target cancer cells in situ. J Breast Cancer 15(3):273–282
Hu YL, Huang B, Zhang TY, Miao PH, Tang GP, Tabata Y et al (2012) Mesenchymal stem cells as a novel carrier for targeted delivery of gene in cancer therapy based on nonviral transfection. Mol Pharm 9(9):2698–2709
Roger M, Clavreul A, Venier-Julienne MC, Passirani C, Sindji L, Schiller P et al (2010) Mesenchymal stem cells as cellular vehicles for delivery of nanoparticles to brain tumors. Biomaterials 31(32):8393–8401
Kang S, Bhang SH, Hwang S, Yoon JK, Song J, Jang HK et al (2015) Mesenchymal stem cells aggregate and deliver gold nanoparticles to tumors for photothermal therapy. ACS Nano 9(10):9678–9690
Sabapathy V, Mentam J, Jacob PM, Kumar S (2015) Noninvasive optical imaging and in vivo cell tracking of indocyanine green labeled human stem cells transplanted at superficial or in-depth tissue of SCID mice. Stem Cells Int 2015:606415
England CG, Ehlerding EB, Cai W (2016) Imaging the biodistribution and performance of transplanted stem cells with PET. J Nucl Med 57(9):1331–1332
Kim T, Lemaster JE, Chen F, Li J, Jokerst JV (2017) Photoacoustic imaging of human mesenchymal stem cells labeled with prussian blue-poly(l-lysine) nanocomplexes. ACS Nano 11(9):9022–9032
Rosenberg JT, Yuan X, Grant S, Teng Ma T (2016) Tracking mesenchymal stem cells using magnetic resonance imaging. Brain Circ 2(3):108–113
Kim J, Chhour P, Hsu J, Litt HI, Ferrari VA, Popovtzer R et al (2017) Use of nanoparticle contrast agents for cell tracking with computed tomography. Bioconjug Chem 28(6):1581–1597
Meir R, Popovtzer R (2018) Cell tracking using gold nanoparticles and computed tomography imaging. Wiley Interdiscip Rev Nanomed Nanobiotechnol 10:e1480
Rabin O, Manuel Perez J, Grimm J, Wojtkiewicz G, Weissleder R (2006) An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles. Nat Mater 5:118–122
Liu Y, Ai K, Liu J, Yuan Q, He Y, Lu L (2012) A high-performance ytterbium-based nanoparticulate contrast agent for in vivo X-ray computed tomography imaging. Angew Chem Int Ed 51:1437–1442
Firouzi M, Poursalehi R, Delavari HH, Saba F, Oghabian MA (2017) Chitosan coated tungsten trioxide nanoparticles as a contrast agent for X-ray computed tomography. Int J Biol Macromol 98:479–485
Kim T, Lee N, Arifin DR, Shats I, Janowski M, Walczak P et al (2017) In vivo micro-CT imaging of human mesenchymal stem cells labeled with gold-poly-l-lysine nanocomplexes. Adv Funct Mater 27(3):1604213
Jackson PA, Rahman WN, Wong CJ, Ackerly T, Geso M (2010) Potential dependent superiority of gold nanoparticles in comparison to iodinated contrast agents. Eur J Radiol 75(1):104–109
Xu C, Tung GA, Sun S (2008) Size and concentration effect of gold nanoparticles on X-ray attenuation as measured on computed tomography. Chem Mater 20(13):4167–4169
Meir R, Shamalov K, Betzer O, Motiei M, Horovitz-Fried M, Yehuda R et al (2015) Nanomedicine for cancer immunotherapy: tracking cancer-specific T-cells in vivo with gold nanoparticles and CT imaging. ACS Nano 9(6):6363–6372
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Nafiujjaman, M., Kim, T. (2020). Gold Nanoparticles as a Computed Tomography Marker for Stem Cell Tracking. In: Basel, M., Bossmann, S. (eds) Cell Tracking. Methods in Molecular Biology, vol 2126. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0364-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0364-2_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0363-5
Online ISBN: 978-1-0716-0364-2
eBook Packages: Springer Protocols