Abstract
Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021
Jin RR, Lin BB, Li DY et al (2014) Superparamagnetic iron oxide nanoparticles for MR imaging and therapy: design considerations and clinical applications. Curr Opin Pharmacol 18:18–27
Kircher MF, Gambhir SS, Grimm J (2011) Noninvasive cell-tracking methods. Nat Rev Clin Oncol 8:677–688
Bulte JW (2009) In vivo MRI cell tracking: clinical studies. Am J Roentgenol 193:314–325
Li L, Jiang W, Luo K et al (2013) Superparamagnetic iron oxide nanoparticles as MRI contrast agents for non-invasive stem cell labeling and tracking. Theranostics 3:595–615
Rogers WJ, Meyer CH, Kramer CM (2006) Technology insight: in vivo cell tracking by use of MRI. Nat Rev Cardiol 3:554
Wang Z, Liu G, Sun J et al (2012) N-alkyl-polyethylenimine stabilized iron oxide nanoparticles as MRI visible transfection agents. J Nanosci Nanotechnol 12:879–886
Liu G, Wang ZY, Lu J et al (2011) Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging. Biomaterials 32:528–537
Liu G, Xia CC, Wang ZY et al (2011) Magnetic resonance imaging probes for labeling of chondrocyte cells. J Mater Sci Mater Med 22:601–606
Xu Y, Wu CQ, Zhu WC et al (2015) Superparamagnetic MRI probes for in vivo tracking of dendritic cell migration with a clinical 3 T scanner. Biomaterials 58:63–71
Chen C, Yu H, Xia R et al (2014) Magnetic resonance tracking of endothelial progenitor cells labeled with alkyl-polyethylenimine 2 kDa/superparamagnetic iron oxide in a mouse lung carcinoma xenograft model. Mol Imaging 13:1–11
Wu CQ, Xu Y, Yang L et al (2015) Negatively charged magnetite nanoparticle clusters as efficient MRI probes for dendritic cell labeling and in vivo tracking. Adv Funct Mater 25:3581–3591
Riemer J, Hoepken HH, Czerwinska H et al (2004) Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal Biochem 331:370–375
Sykova E, Jendelova P, Herynek V (2011) Magnetic resonance imaging of stem cell migration. Methods Mol Biol 750:79–90
Acknowledgement
We gratefully acknowledge financial support from the Funds for Creative Research Groups of China (81621003) and National Key Basic Research Program of China (2013CB933903).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zhu, W., Xu, Y., Jin, R., Wu, C., Ai, H. (2020). MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles. In: Basel, M., Bossmann, S. (eds) Cell Tracking. Methods in Molecular Biology, vol 2126. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0364-2_10
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0364-2_10
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0363-5
Online ISBN: 978-1-0716-0364-2
eBook Packages: Springer Protocols