Abstract
Natural acellular matrices obtained from decellularization procedures are biocompatible and non-immunogenic materials considered promising tools for regenerative medicine purposes. Before in vivo implantation, these matrices must be efficiently decellularized, removing all the cellular components to avoid any immunogenic reaction. At the same time, it is important to maintain the original three-dimensional structure of the specific tissue. Here we describe a method: (1) to decellularize mouse quadriceps using a detergent-enzymatic treatment (DET) and (2) to assess decellularization efficiency and scaffold properties.
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Acknowledgments
Piccoli M and Franzin C are supported by Istituto di Ricerca Pediatrica Città della Speranza, Grant Number 16/02; Trevisan C and Maghin E are supported by Istituto di Ricerca Pediatrica Città della Speranza. Pozzobon M is supported by University of Padova, Grant number GRIC15AIPF, Assegno di ricerca Senior. Franzin C, Piccoli M, and Pozzobon M are coinventors of Italian Patent N. 0001422436 entitled “Matrice acellulare per ricostruzione in vivo di muscolo scheletrico.”
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Piccoli, M., Trevisan, C., Maghin, E., Franzin, C., Pozzobon, M. (2017). Mouse Skeletal Muscle Decellularization. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_28
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DOI: https://doi.org/10.1007/7651_2017_28
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