Abstract
Mechanisms of soil organic carbon (SOC) stabilization has received much focus recently due to its relevance in controlling the global carbon (C) cycle. Clay minerals are known to stabilize SOC through mechanisms such as, ligand exchange, polyvalent cation bridging, electrostatic attraction, H-bonding, and van der Waals forces. Most studies focused on clay organic interactions derived from geological deposits. However, the effect of pedogenic clay on SOC stability is still lacking especially in tropical conditions like India. Therefore, the impact of clay with different mineralogy such as smectite, 2:1 interstratified minerals, illite, kaolinite on soil C mineralization, and labile C fractions in four distinct soils under natural conditions was evaluated. The results indicated that the cumulative C mineralization (CO2-Ccum) was the highest in Mollisol (0.97 g C kg-1) and Vertisol (0.96g C kg-1), which was dominated by kaolinite+illite+chlorite–interstratified minerals and smectite/2:1 interstratified minerals, respectively followed by Alfisol and Inceptisol, which was dominated by kaolinite and illite. The percentage of SOC loss showed opposite trend where the highest SOC loss was accounted in Inceptisol (10.1%) and Alfisol (9.02%) whereas Mollisol and Vertisol lost lowest amount of SOC. Labile C fractions and dehydrogenase activity were significantly higher in Mollisol and Vertisol over Alfisol and Inceptisol. Specific surface area (SSA) (r = 0.65, P ≤ 0.05) and cation exchange capacity (CEC) (r = 0.62, P ≤ 0.05) positively correlated with C mineralization and labile C fractions and negatively correlated with percentage SOC loss. Principal component analysis confirmed that varying mineralogy significantly influenced the sequestration of labile C in soil under natural conditions. This study highlighted the positive influence of 2:1 expanding/limited expanding clay mineralogy in sequestering and stabilizing labile C in soil.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
1 Introduction
In terrestrial ecosystem, protection of soil organic carbon (SOC) plays a central role in carbon (C) sequestration. Understanding the process of SOC turnover can provide insights on how to tackle global warming in this changing climate. The underlying mechanisms governing the storage and protection of SOC in soil are influenced by natural as well as management-induced changes (Dhaliwal et al. 2020; Piazza et al. 2020). In terrestrial system, the soil stores around 1550 Pg of C which is roughly two times its concentration in atmosphere i.e., 780 Pg (Lal 2008), 80% of which is actively involved in global C cycle (Singh et al. 2017). The organic C (OC) present in soil ranges from easily decomposable simple carbohydrates to complex aromatic hydrocarbons. Based on their mean residence period and turnover rates, the soil C could be defined as labile and recalcitrant. Mineralization of stored SOC with time is a function of prevailing climate (Tisdall 1996), soil temperature and moisture (Singh et al. 2017), soil texture (Baldock 2007), organic residue (Roychand and Marschner 2013), composition and strength of organo-mineral associations (Sarkar et al. 2018; Singh et al. 2016), and land use patterns (Hassink 1994). Among these factors, the organo-mineral associations are thought to be the strongest and most significant (Singh et al. 2018).
SOC loss via heterotrophic respiration is one of the major contributors for increasing the atmospheric CO2 concentration (Lal 2008). Even though mineralization is necessary for nutrient cycling in soil, which provides nutrition to crops, uncontrolled mineralization is causing global temperature to rise at an alarming rate. Much research has previously focused on how environmental factors, such as temperature and moisture affect heterotrophic soil respiration (Dan et al. 2016; Suseela et al. 2012; Larionova et al. 2017) but mineral composition also plays an important role in soil C stabilization. It is assumed that coarse textured soil may have high susceptibility to soil C respiration than their fine textured counterpart (Conant et al. 2008; Lal 2007; Nguyen and Marschner 2014). Clay minerals in soils offer an essential restriction to OC mineralization based on their composition, content (Feng et al. 2005; Kahle et al. 2003), and other surface properties (Singh et al. 2018). With difference in clay mineralogy the labile C fractions like dissolved organic carbon (DOC) showed different tenacity and binding (Singh et al. 2016). Clay minerals having higher specific surface areas (SSA) and cation exchange capacities (CEC) and iron/aluminium (Fe/Al) oxides are reported to protect C in soil by reducing C mineralization (Singh et al. 2018). Soils with a similar texture but a higher concentration of smectitic and allophanic clays retained more C than soils with kaolinite or vermiculite minerals (Saggar et al. 1996, 1999). The fundamental difference in mineralogical composition may influence how these minerals behave in different natural environment in terms of soil C retention. Smectite minerals are expanding in nature having 2:1 layer structure, a higher CEC, SSA, and permanent negative charge whereas kaolinitic clays are non-expanding having 1:1 layer structure with lower CEC, SSA, and pH-dependant charge. In terms of surface properties, illitic clays are intermediate between smectite and kaolinite with non/limited expansion capabilities. Apart from these, the presence of sesquioxides in soil clay fractions has a significant impact on clay surface properties due to their higher SSA (Saidy et al. 2013; Singh et al. 2018). Mechanism through which minerals interact with organic matter (OM) can also be different. Ligand exchange and cation bridging can result in strong bonding between clay minerals and OC moieties whereas hydrogen bonding, van der Waals forces and proton interactions could also establish some weak interactions (Rashad et al. 2010; Singh et al. 2018).
Despite many previous studies focused on how clay organic interaction influences the retention of OC in soils, they were based on refined clay minerals from geological deposits (Feng et al. 2005; Rashad et al. 2010; Saidy et al. 2013). Pedogenic clays, on the other hand, behave quite differently. Clay behavior may differ dynamically under the influence of natural environment as opposed to a controlled scenario. They undergo intense weathering process, changing their particle size. Having OM or sesquioxides coatings may influence their surface properties like SSA and CEC drastically (Churchman and Lowe 2012). The presence of cations in natural and processed clay environments may differ significantly which might influence their interactions. Large areas of land in India are covered under the soil orders namely Vertisol, Mollisol, Inceptisol and Alfisol. Under tropical conditions, these soils undergo continuous hydrothermal changes resulting in widely different clay mineralogy and varying cementing agent contents such as OM and sesquioxides which ultimately affect the SOC retention and mineralization (Chatterjee et al. 2013). As SOC is approximately three times that of atmospheric and terrestrial pools (Schmidt et al. 2011), even minor changes in soil C stock will result in a significant change in atmospheric C concentration. Temperature and climate affect SOC mineralization on macro scale; however, on microscale, the influence of mineralogy cannot be overlooked. Barré et al. (2014) previously reviewed the impact of phyllosilicates on SOC stabilization, but there is little information about the stabilization potential of other minerals. Furthermore, intensive crop management gradually alters clay characteristics, affecting soil C storage capacity (Das et al. 2019a, b), so assessing clays of natural origin can help us evaluate them from a common reference. Keeping this in view, this study was conducted to know the effect of varying clay mineralogy on SOC dynamics in four different natural soils with the understanding that natural pedogenic clays will behave differently than processed clays. It was conceived with the following objectives (i) to examine SOC mineralization as influenced by mineralogy, (ii) to compare the effects of different clay mineralogy on labile C fractions, and (iii) to establish relationship between SOC mineralization and labile C fractions with different surface properties of clays like SSA, CEC, and clay content.
2 Materials and Methods
2.1 Soil Collection, Processing, and Determination of Initial Properties
Soil samples were collected from four different regions, each representing a different soil order according to the US soil taxonomy (Fig. 1). The soils collected from Jabalpur, Madhya Pradesh were formed under dry and sub-humid climate, belonged to Typic Haplusterts with clay texture and neutral reaction. This region experiences annual rainfall of 1386 mm with an average temperature of 25°C (Koppen climate classification, Csa) (Peel et al. 2007). Second soil sample was collected from Pantnagar, Uttarakhand, that belonged to Aquic Hapludoll with silty loam texture having neutral reaction. Climatically, the area is sub-humid subtropical with hot humid summers and severe cold winters with an average annual rainfall measuring around 1383 mm and relative humidity of 90–95% during the rainy season (Koppen climate classification, Cfa) (Peel et al. 2007). Soils of New Delhi belonged to Typic Ustocherpt with sandy loam texture having neutral reaction, with average annual temperature of 29°C and rainfall of 790 mm. New Delhi has a semi-arid subtropical climate with hot dry summers and cool winters (Koppen climate classification, BSh) (Peel et al. 2007). Ranchi (Jharkhand) soils formed under sub-humid climate with severe hot, dry summer, and cool winter, belonged to Typic Haplustalf with clay loam texture and acidic reaction. It experiences average annual temperature of 24°C with average rainfall of 1450 mm (Koppen climate classification, Cwa) (Peel et al. 2007). In each location, soil samples were collected to a depth of 0−20 cm from five randomly selected spots under natural vegetation in 2018. One part of the freshly collected sample were pooled together and kept in in a refrigerator at 4°C for analysis of microbial biomass carbon (MBC), dehydrogenase activity (DHA), and C mineralization study whereas the other part was air dried, ground, mixed thoroughly, and passed through 2mm sieve to obtain a homogeneous composite sample through quartering process for analysis of initial soil parameters, soil C, and its fractions. The texture analysis was done by modified dispersion and sedimentation method (Kettler et al. 2001). Chemical attributes of soil, viz., pH and electrical conductivity (Page et al. 1982), easily oxidizable organic C (WBC) (Walkley and Black 1934), available nitrogen (Subbiah and Asija 1956), available phosphorus (Bray and Kurtz 1945; Olsen et al. 1954), and available potassium (Page et al. 1982) were determined (Table 1).
2.2 Clay Mineralogy and Surface Properties of Clays
As described in previous section, in each location soil samples from five randomly selected spots were pooled together, air dried and thoroughly mixed to study clay mineralogy. The clay sized particles (< 2 μm) of soil were separated by the procedure outlined by Jackson (1985). Extracted clays underwent four treatments, viz., (a) Mg-saturation and air drying, (b) Mg-saturation and glycerol solvation, (c) K-saturation and air drying, and (d) K-saturation followed by heating at 550 °C and arranged basally for X-ray diffraction (XRD) analysis. The XRD plots of the basally oriented samples were obtained in a Phillips X-ray diffractometer (PW 1710 diffractometer control, PW 1729 X-ray generator) with automated powder diffractometer (APD) software using Ni-filtered Cu-Kα radiation (λ, 0.154184 nm) at a scanning speed of 1.5°2θ min−1. Semi-quantification of the clay minerals was done from the diffractograms of Mg-saturated and glycerol-solvated samples by the “peak area measurement” options of the APD software as per Gjems (1967) and Datta et al. (2015) (Fig. 2). Clay minerals were identified and categorized based on the observations of earlier researchers (Barré et al. 2008; Datta et al. 2015; Das et al. 2019a, b, 2022; Moore and Reynolds 1989). Peaks with C-axis spacing of ~1.8 nm correspond to smectite, one or more peaks observed adjacent to smectite peaks which might signify the presence of smectite interstratified with vermiculite, hydroxyl interlayer minerals (HIM), and other 2:1 minerals (Das et al. 2019a, b, 2022) was broadly categorized as 2:1 interstratified minerals. The presence of chlorite in Mollisol, Inceptisol, and Vertisol was confirmed after heating the K saturated clays at 550 °C where only the peak with c-axis spacing ~1.4 nm was reinforced and categorized as chlorite and chlorite-interstratified minerals. Peaks with C-axis spacing of ~1.01 and ~0.72 nm correspond to illite and kaolinite, respectively. Some broad peaks adjacent to illite were considered as illite-interstratified minerals. SSA of clays isolated from soils were determined by ethylene glycol monoethyl ether (EGME) method (Carter et al. 1965) and CEC of isolated clays were determined by N-ammonium acetate (pH 7) method outlined by Jackson (1985).
2.3 Carbon Mineralization Study
Carbon mineralization in the form of soil respiration was measured periodically by alkali trap method (Anderson 1982). Fresh soils were preincubated at 25°C for 24 h prior to incubation to acclimatize microbes to laboratory incubation conditions. In brief, 20 g soil was placed in a 500 mL mason glass jar and incubated at a constant temperature (25°C) and water content (60% of water holding capacity). C mineralization was measured using a set of 12 tightly capped mason jars containing four different soils with three replications. The moisture content of the soil in the jars was kept constant by measuring weight loss periodically and adding deionized water as needed. To trap CO2, 5 mL of 1 N sodium hydroxide (NaOH) solution were kept inside small vials (10 mL, with no lids) within the jar. The CO2 trapped NaOH solutions were taken out of the jar at each sampling day and was back titrated with 0.5 N hydrochloric acid (HCl) in presence of barium chloride (BaCl2) using phenolphthalein indicator. After 3, 7, 14, 21, and 28 days of incubation, small NaOH vials were removed and replaced with a fresh set of NaOH vials, and jars were returned to the incubator. The quantity of C mineralized at each sampling day was summed over 28-day incubation period to express as cumulative CO2-C mineralization (CO2-Ccum).
C mineralization was computed by the following formula:
CO2- C mineralized (mg g−1 soil) = \(\frac{\left(B - S\right) \times N \times 6}{W}\)where B is the volume of HCl used in blank sample (mL), S is the volume of HCl used in soil sample (mL), N is the normality of HCl, and W is the weight of soil sample (g).
2.4 Labile Carbon Fractions
2.4.1 Soil Organic Carbon (SOC)
Total soil carbon (TC) was measured by CHNS analyser (Euro Vector make, EuroEA3000 model), while total OC or SOC was calculated by subtracting inorganic C (measured as per methods outlined by Snyder and Trofymow 1984) from TC.
2.4.2 Microbial Biomass Carbon (MBC)
Soil MBC was estimated by the substrate induced respiration method (Bailey et al. 2007) using a gas chromatograph (Make Agilent, Model GC-4890) and the following equation of Anderson and Domsch (1978):
where x = microbial biomass C (mg kg-1) and y = rate of CO2 evolution (mL CO2 kg-1 soil h-1)
2.4.3 Dissolved Organic Carbon (DOC)
The DOC was extracted by the methods outlined by Jones and Willett (2006). Here, 5g of dry soil was shaken (200 rpm) in a reciprocal shaker for 1 h with 25 mL ultra-pure water and then centrifuged at 13,000 rpm for 30 min at 4°C. The supernatant was passed through a 0.45 µm glass fiber filter and the C content in the supernatant was estimated following the procedure given by Synder and Trofymow (1984).
2.4.4 Potassium Permanganate Oxidizable Carbon (KMnO4-C)
Active C oxidizable by KMnO4 was determined by modified Blair method as outlined by Weil et al. (2003). In this method, dilute and slightly alkaline KMnO4 reacted with the most readily oxidizable forms of soil C, converting Mn (VII) to Mn (II), and the absorbance was measured by a colorimeter at 550 nm. Oxidizable C was computed using following equation:
KMnO4 oxidizable C (mg kg-1) = [0.02mol/L − (a + b ×absorbance)] × (9000mg C/mol) × (0.02 L solution/0.005 kg soil).
where 0.02 mol/L is the initial solution concentration, a is the intercept and b is the slope of the standard curve, 9000 is the mg C (0.75 mol) oxidized by 1 mol of MnO4 changing from (Mn7+→ Mn2+), 0.02 L is the volume of KMnO4 solution reacted, and 0.005 is the kg of soil used.
2.4.5 Particulate Organic Matter Carbon (POM-C)
The particulate organic matter (POM) was extracted from the soil following the procedure outlined by Cambardella and Elliott (1992) and C content in POM was determined by dry combustion method in a CHNS analyser (Euro Vector make, EuroEA3000 model).
2.4.6 Complex Organic Matter Carbon (COM-C)
The content of complexed organic matter carbon (COM-C) was determined from the difference between the SOC pool and the POM-C pool.
2.5 Statistical Analysis
The data obtained from the above measured parameters were statistically analyzed by one-way analysis of variance (ANOVA). For statistical significance, the treatment means were differentiated following a posthoc Tukey’s test with honest significant difference (HSD) at 95% confidence value (p< 0.05) using the SPSS (version 16.0) software by taking clay mineralogy (soil orders) as a factor. Pearson’s correlation among the C fractions and clay parameters were performed using the “corr_coef” function and the correlation plot constructed using the “corr_plot” function of “metan” package (Olivoto and Lúcio 2020) in R studio. Principal component analysis (PCA) was done to find out the traits or parameters explaining more variation in the system and the relationship of individual parameters with soil orders. PCA was performed in R studio using packages “FactoMineR” (Lê et al. 2008) and “factoextra” (Kassambara and Mundt 2017). All the figures were drawn using Microsoft excel (2016).
3 Results
3.1 Mineralogy of Different Soil Samples
The mineralogical composition of different soil samples (Table 2) showed that Vertisol contained smectite and 2:1 interstratified minerals as major minerals constituting 25.4 and 41.5 percent (%), respectively. Overall, 67% of minerals present in Vertisol belonged to smectite/2:1 interstratified minerals. Kaolinite dominated the mineralogical composition (54.5%) in Mollisol but other minerals such as chlorite and chlorite-interstratified mineral, illite, illite-interstratified mineral, and smectite were also present with 16.1, 20.9, 6.89, and 1.61%, respectively accounting for 45.5% of the total mineralogical composition. Inceptisol mineralogical make up showed the presence of illite rich interstratified minerals as the major fraction followed by kaolinite contributing 58.7 and 35.2%, respectively. In Alfisol, kaolinite dominated the mineralogical make up having 76.9% followed by illite having 17.2%, respectively.
3.2 Effect of Clay Mineralogy on Surface Properties of Different Clays Isolated from Soil Samples
SSA and CEC of soil clays showed significant variations among different clays (Table 3). Vertisol clays showed the highest SSA of 506 (m2 g-1 clay) followed by Mollisol, Inceptisol, and Alfisol clays having 149, 50.9, and 38.2 (m2 g-1 clay), respectively. For CEC, a similar trend was followed where clays isolated from Vertisol showed significantly high amount of CEC i.e., 81.3 (m eq 100 g-1) whereas Alfisol clays showed the lowest CEC of 33 (m eq 100 g-1). Clay content was found to be highest in Vertisol having 58.4% clay followed by Alfisol, Mollisol, and Inceptisol.
3.3 Effect of Clay Mineralogy on SOC Mineralization
Carbon mineralization was found to be significantly affected by clay mineralogy. Respiration rates were invariably higher in Mollisol and Vertisol soils throughout the incubation period (Fig. 3). After 28 days of incubation, Mollisol and Vertisol showed CO2-Ccum of 0.97 and 0.96 g C kg-1, respectively being statistically at par (Fig. 4a). Inceptisol showed the lowest CO2-Ccum of 0.84 g C kg-1. The percentage of SOC lost as CO2 (%) showed the opposite trend compared to the total C mineralization from soils (Fig. 4b). Mollisol and Vertisol showed significantly lower percentage loss of C lost as CO2 from total SOC compared to other soils. Inceptisol showed the highest loss of 10.1% as CO2 from SOC.
3.4 Effect of Clay Mineralogy on Labile Carbon Fractions and Enzymatic Activities
Difference in clay mineralogy significantly affected the labile C fractions in soils as well (Table 3). With respect to fractions like MBC, DOC, KMnO4-C, and SOC, Mollisol soils showed the highest amount followed by Vertisol, whereas Alfisol and Inceptisol showed the lowest amount. In WBC, Mollisol showed the highest amount followed by Vertisol, Inceptisol, and Alfisol. SOC was partitioned into POM-C and COM-C (Fig. 5). POM-C was observed to be highest in Mollisol, which was significantly higher than Vertisol, Inceptisol and Alfisol. With respect to COM-C, Mollisol and Vertisol were statistically at par, whereas lowest amount was found in Inceptisol. For evaluation of overall enzymatic conditions of soil, DHA was determined. Mollisol and Vertisol exhibited the highest DHA whereas Inceptisol showed the lowest activity (Table 3).
3.5 Relationship Between Soil Clay Characteristics with SOC Fractions and Mineralization
Correlation studies indicated that all the soil chemical and biological properties exhibited a highly positive correlation (p<0.05 and p<0.01) among each other and CO2-Ccum (Fig. 6). C mineralization showed strong and significant correlation with labile fractions, such as MBC, KMnO4-C, POM-C, WBC, DOC, SOC, and COM-C. Irrespective of soil clay composition, SOC, POM-C, COM-C, and WBC exhibited significant positive linear relations with CO2-Ccum (mg CO2-C kg-1 soil), where the R2 values were in order of SOC followed by COM-C, WBC, and POM-C (Fig. 7). Clay characteristics like SSA, CEC, and clay content showed significant correlation among themselves. SSA had significant positive correlation with CO2-Ccum, COM-C, and DOC, whereas CEC showed significant positive correlation with COM-C, CO2-Ccum, DOC, and SOC. Both SSA and CEC had significant negative correlation with percent C loss.
3.6 Principal Component Analysis
Principal component analysis (PCA) in form of two dimensional bi-plot (loading and score plots) revealed that up to 90.9% of cumulative variability in soil physico-chemical and clay properties due to difference in mineralogy can be explained by two principal components (Table 4). As shown in the biplot, the parameters clustered distinctly under two dominant principal components, PC1 and PC2, which explained 73.4% and 17.5% of the total variability with an eigenvalue >1 (Table 4, Fig. 8). The factor loading values in component matrix (Table 5) showed that PC1 had large positive loadings on SOC followed by CO2-Ccum, COM-C, POM-C, and WBC, respectively, and exhibited a highly positive correlation with each other. DOC, MBC, KMnO4-C, and DHA had also higher loading on PC1. The C loss (%) showed negative loadings on PC1. Remarkably, clay content, SSA and CEC had strong loadings on PC2 as evident in Table 5 and Fig. 8. In the biplot, Mollisol showed distinct and close affinity to all the labile C fractions and microbial properties, whereas Vertisol showed more affinity towards surface properties of clays. Alfisol and Inceptisol formed a distinct group showing close proximity towards C loss (%).
4 Discussion
In this study, mineralogy of the soil reaffirms the influence of parent material, climate and topography. It was evident that mineralogy of Vertisol was dominated by smectite, 2:1 interstratified minerals. Previous studies have highlighted that in humid tropics, continuous supply of bases from Ca-rich zeolites helped in stabilizing these smectite in Indian soil (Bhattacharyya et al. 1993). In majority of Indian Vertisol found in semi-arid and sub-humid regions, the presence of smectite and kaolinite is quite common (Bhattacharyya et al. 1993; Pal 2003; Pillai et al. 1996). In Mollisol, chlorite, illite, and illite-interstratified minerals were present in significant amount even though highest was kaolinite. Usually, 2:1 phyllosilicates are predominantly found in Mollisol because of their formation in drier climate compared to Ultisol or Alfisol. However, there is a possibility of Mollisol having kaolinitic minerals in tropical climate (Allen and Fanning 1983). Previously, kaolinite and illite minerals were found in appreciable amount in the soils of this area (Samra 1982). Some studies have identified kaolinite as dominant mineral followed by interstratified mineralogy with traces of chlorite in soils developed on granite in Shivalik regions (Surya et al. 2015). In sub-humid subtropical climate of this region where summer temperature crosses > 40 °C having good drainage and higher OM accumulation, higher moisture/rock ratio, might lower the Si4+ concentration along with other basic cations hastening chemical weathering process. This process could have hastened the formation of the kaolinite directly from the silicate minerals (Grim 1968). The presence of chlorite also highlighted the intermediate stage of weathering of the soil. The role of vegetation on alteration and neo-synthesis of minerals is also important. The presence of high OM, wet moisture regime with high leaching rates and high above and below ground root biomass with rich biodiversity has a definite role to play in shaping the alteration of mica into chlorite and/or kaolinite (Sharma 1982; Singhal and Sharma 1983). In Inceptisol, majority of mineral suite belonged to illite/illite-interstratified mineral. Inceptisol are predominantly found in regions subjected to moderately intensive pedogenic processes (Allen and Fanning 1983). This mineralogy indicated relative immaturity and presence of weatherable minerals (Pal 2017) as the parent materials are devoid of products of advanced stages of weathering (Allen and Fanning 1983). Some studies have found kaolinite as dominant mineral in Inceptisol of eastern India (Das et al. 2018). However, our finding corroborated with previous findings (Das et al. 2018, 2019a, b) where mica was found to be the dominant mineral fraction. Kaolinite dominated the mineralogical composition of Alfisol. Similar mineralogical composition was also previously found (Das et al. 2019a, b; Pal et al. 2003, 2017). Under the influence of humid sub-tropical climate, due to availability of high temperature and moisture, the parent material might have been transformed with time into illite/interstratified mica followed by vermiculite, smectite and finally into kaolinite by chemical weathering (Allen and Fanning 1983). Difference in mineralogy influenced the surface properties of clays significantly. Smectitic clays having 2:1 expanding silicate structure possess permanent negative charge which imparts higher SSA and CEC whereas kaolinite clays have 1:1 non expanding layer structure having small amount of pH dependent charge surface possessing low SSA and CEC. Illitic clays have limited expanding properties that expresses SSA and CEC higher than kaolinite but lower than smectite (Churchman and Lowe 2012). We employed EGME to determine the SSA, which has characteristics to penetrate internal surfaces of minerals to measure both internal and external surface areas (Ugochukwu 2017). In our study, we found Vertisol clays had higher amount of smectite and 2:1 interstratified minerals, which collectively expressed higher amount of SSA and CEC. The surface area, as well as CEC, decreased with increase in low activity, non/limited expanding clays like illite and kaolinite. Mollisol having relatively good amount of mixture of illite, chlorite and interstratified minerals showed SSA and CEC higher than Inceptisol and Alfisol clays. The trend of SSA and CEC was smectitic + 2:1 interstratified minerals > kaolinite + illite + chlorite mixed clay minerals > illite dominating minerals > kaolinitic clay minerals which is in conformity with several previous reports (Saidy et al. 2013; Singh et al. 2016, 2018; Ugochukwu 2017).
The effect of mineralogy on SOC mineralization and labile C sequestration were significant. It was observed that greater percentage of SOC is lost from Inceptisol, which is at odds with CO2-Ccum, which were greater in Mollisol and Vertisol after 28 days of incubation (Fig. 4). The higher amount of C mineralization in Mollisol could be due to the higher amount of easily oxidizable C present initially in this soil that showed higher accumulation of fresh OM contributed to higher mineralization (Haynes 2005). The rapid decay of C in Mollisol and Vertisol might be due to the higher porosity in these soils because of fine texture, which maintained more water (Baldock and Skjemstad 2000; Chen et al. 2014). Better texture, porosity, higher microbial activity along with higher amount of available C in soil may have facilitated greater rate of mineralization than other soils (Singh et al. 2017; Six et al. 2002). However, the trends for C mineralization expressed in percentage loss of initial amounts of SOC followed illite dominated clays (Inceptisol) > kaolinite dominated clays (Alfisol) > smectitic+vermiculite clay minerals (Vertisol) > kaolinite+illite mixed clay minerals (Mollisol) although Vertisol and Mollisol showed no significant difference. Mollisol and Vertisol stabilized more SOC than other soils. These results highlighted the effect of mineralogy in protecting the C in soil. The effect of surface properties of clays was evident from the significant negative correlation among surface properties of clays and C lost as CO2 (Fig. 6). Higher SSA and CEC in smectite-dominated soil resulted in stronger adsorption of OC by ligand exchange or cation bridging and protected SOC from mineralization (Sarkar et al. 2018; Singh et al. 2016, 2018). In this study, Vertisol and Mollisol expressed higher labile C in terms of MBC, DOC, KMnO4-C and WBC, respectively, while Inceptisol and Alfisol showed the lowest amount (Table 3). The association of OM with mineral particles are affected by the mineralogy because of the difference in SSA and charge characteristics (Feng et al. 2013). Smectitic minerals due to their small size and high charge density could have helped in binding of OM strongly via mechanisms like ligand exchange, cation bridging, hydrophobic interaction, or van der Waals forces (Churchman and Lowe 2012; Singh et al. 2018). Smectite help in formation of micro-aggregates in fine textured soil where OM may get entrapped and remain protected as slowly available C pool for a long period whereas soils having 1:1/ non-expanding clays with lower SSA and CEC does not offer any protection to SOM (Baldock and Skjemstad 2000; Six et al. 2002). Many experiments have shown that “sorption” mechanism significantly affects the retention of OM in soil by protecting it from biodegradation (Arnarson and Keil 2000; Kalbitz et al. 2005; Singh et al. 2016), yet it can release them into solution in a systematic manner through particle associated OM (Kaiser and Guggenberger 2003). Our findings confirmed the effect of clay minerals in enrichment of labile C in soils, which are in line with previous findings (Nguyen and Marschner 2014; Saidy et al. 2012; Singh et al. 2016). Vertisol and Mollisol soils were richer in clay content as well as dominant in 2:1 type fine smectititic minerals (Gupta et al. 1999) than either Inceptisol and Alfisol which are poorer in clay content and dominated by 1:1 type clay minerals like illite and kaolinite. This could also play a role in SOC stabilization, which in turn enhanced the SOC to a greater extent (Nguyen and Marschner 2014). However, in temperate climate, Mayes et al. (2012) found that under low DOC concentrations, Ultisol and Alfisol exerted high adsorption capacity compared to Mollisol and a major role was played by textural clay and Fe oxide content. Similarly, a study consisting of 52 temperate mineral soil samples from podzol, volcanic as well as A and B horizons in Canada observed a dominant relationship between non-crystalline Al and Fe oxides and DOC adsorption rather than clay content (Kothawala et al. 2009). These researchers noted that the coatings of Fe and Al oxides masked the effect of clay content. However, in our case, clay content, SSA, and CEC influenced the accumulation and stabilization of SOC. Higher WBC in Mollisol than Vertisol could be attributed to the difference in average annual temperature and texture that exist between these two soils which might have impacted significantly the differential build-up of SOC in these soils (Surya et al. 2015). Due to higher availability of labile substrates and higher microbial biomass, the enzyme activity also followed the similar trend. The POM-C was higher in Mollisol and Vertisol compared to other soils. POM-C are essentially the plant−derived remains that are predominant in macro aggregates fraction due to their strong association with mineral particles (Six et al. 1998; Wright et al. 1999). In our study, Vertisol and Mollisol had fine texture soil along with 2:1 phyllosilicate mineralogy, which provided perfect microsites for microbial growth. Due to higher microbial secretions along with better soil aggregation, OM could have occluded into aggregates, which in turn sequestered more POM-C in soil compared to coarse textured soils having 1:1 type of mineralogy (Cotrufo et al. 2015; Schmidt et al. 2011; Schweizer et al. 2021; Steffens et al. 2017). The results of COM-C also highlighted the importance of 2:1 mineralogy of Vertisol in preferentially accumulating the OM of complex nature, viz., aromatic and phenolic compounds (Leinweber et al. 1999; Wattel-Koekkoek et al. 2001).
Correlation study among the labile C fractions and surface clay properties highlighted their strong interconnection among each other (Fig. 6). SOC showed a strong and significant relationship with CO2-Ccum, MBC, DOC, KMnO4-C, POM-C, COM-C, WBC, and DHA suggesting that change in SOC storage greatly depends upon these pools. Similar results were previously reported (Bongiorno et al. 2019; Rudrappa et al. 2006; Souza et al. 2016). Due to strong correlation, any of the above fractions could be used as potential indicators of SOC build up in the studied area. As the interplay of SOC with other fraction is very dynamic and responsive, therefore a strong positive influence of SOC, POM-C, COM-C, and WBC on C mineralization was observed (Fig. 7). Strong correlation between CO2-Ccum and DHA showed that the microbial populations are actively contributing to respiration due to higher substrate availability. The higher correlation coefficient between SOC and POM-C than SOC and MBC suggested that the newer organic materials contributed to the POM largely. SSA and CEC showed significant correlation with SOC, CO2-Ccum, and DOC highlighting the importance of clay surface characteristics in C accumulation. Higher surface areas and CEC helped in accumulating more OC through mechanisms like ligand exchange, cation bridging, and van der Waals interaction which has been previously reported (Dontsova and Bigham 2005; Saidy et al. 2013). Singh et al. (2016) highlighted that SSA was the most crucial factor in adsorption and stabilization DOC in clays. In addition to correlation studies, PCA analysis revealed that Mollisol accumulated higher amount of labile C fractions (Fig. 8). The prevalence of temperate sub-humid climate helped in accumulation of fresh OM in terai regions of Pantnagar, which is why the WBC and SOC were quite higher in this soil. However, Vertisol had a higher affinity for SSA, CEC, and clay content indicating that clay properties significantly influenced SOC enrichment in this soil. Interestingly, Inceptisol and Alfisol formed a distinct group showing higher percentage of SOC loss. This similarity in behavior of both these soils despite their occurrence in two different sets of climatic conditions could be attributed to the clay characteristics. Both soils contained limited to non-expanding clays such as illite and kaolinite, which behaved similarly in terms of surface properties. Therefore, they sequestered less labile C and a higher percentage of C was lost as CO2.
5 Conclusions
This study inferred that variation in pedogenic clay had a significant effect on stabilization of soil organic carbon. Higher cumulative mineralization and labile C fractions such as microbial biomass carbon, dissolved organic carbon, KMnO4-C, particulate organic matter carbon, complex organic matter carbon along with dehydrogenase activity, and lower percentage of soil organic carbon loss were found in Mollisol and Vertisol soils than Inceptisol and Alfisol. Variation in CO2 evolution through mineralization could be largely explained by soil organic carbon and complex organic matter carbon. Hence, under natural conditions, inclusion of these parameters will better reflect the process of soil respiration in future study. This study validated the clear influence of 2:1 mineralogy in terms of specific surface area and cation exchange capacity on labile C enrichment in Vertisol, whereas the presence of inherently higher amounts of soil organic carbon could be the predominant factor governing higher carbon sequestration in Mollisol. Stronger correlation between labile C fractions and clay properties revealed that greater specific surface area and cation exchange capacity would result in greater C sequestration and stabilization in soil. This study found that presence of limited to non-expanding clay mineralogy in Inceptisol and Alfisol is more important for soil C sequestration and stabilization than climate. This preliminary information will help future researchers to consider clay characteristics while performing soil carbon studies. As the ability of soil to store organic carbon varies according to climate-soil-management interactions, it will be worthwhile to confirm these findings by studying other soils from different agro-climatic regions.
Data availability
Data may be available on request to corresponding author.
Abbreviations
- C:
-
Carbon
- OC:
-
Organic carbon
- SOC:
-
Soil organic carbon
- OM:
-
Organic matter
- Pg:
-
Peta gram
- DOC:
-
Dissolved organic carbon
- SSA:
-
Specific surface area
- CEC:
-
Cation exchange capacity
- MBC:
-
Microbial biomass carbon
- DHA:
-
Dehydrogenase activity
- XRD:
-
X-ray diffraction
- WBC:
-
Walkley black carbon
- APD:
-
Automated powder diffractometer
- POM:
-
Particulate organic matter
- COM:
-
Complex organic matter
- PCA:
-
Principal component analysis
- HIV:
-
Hydroxy interlayered vermiculite
- CO2-Ccum :
-
Cumulative carbon mineralization
- EGME:
-
Ethylene glycol monoethyl ether
References
Allen BL, Fanning, DS (1983) Composition and soil genesis. Pedogenesis and soil taxonomy/edited by LP Wilding, NE Smeck, and GF Hall.
Anderson JPE (1982) Soil respiration In: Black CA (ed) Methods of soil analysis, part 2 (2nd edn). Agronomy Monograph 9: 831–871 https://doi.org/10.2134/agronmonogr9.2.2ed.c41
Anderson JP, Domsch KH (1978) A physiological method for the quantitative measurement of microbial biomass in soils. Soil BiolBiochem 10:215–221. https://doi.org/10.1016/0038-0717(78)90099-8
Arnarson TS, Keil RG (2000) Mechanisms of pore water organic matter adsorption to montmorillonite. Mar Chem 71:309–320. https://doi.org/10.1016/S0304-4203(00)00059-1
Bailey VL, Bolton H, Smith JL (2007) Substrate-induced respiration and selective inhibition as measures of microbial biomass in soils. Soil sampling and methods of analysis. CRC Press, Boca Raton, Florida, USA, pp 515–526
Baldock JA (2007) Composition and cycling of organic soil carbon in soil. In: Marschner, P., Rengel, Z. (Eds.), Nutrient Cycling in Terrestrial Ecosystems. Springer-Verlag, Berlin Heidelberg. https://doi.org/10.1007/978-3-540-68027-7_1
Baldock JA, Skjemstad JO (2000) Role of the soil matrix and minerals in protecting natural organic materials against biological attack. Org Geochem 31:697–710. https://doi.org/10.1016/S0146-6380(00)00049-8
Barré P, Velde B, Fontaine C, Catel N, Abbadie L (2008) Which 2: 1 clay minerals are involved in the soil potassium reservoir? Insights from potassium addition or removal experiments on three temperate grassland soil clay assemblages. Geoderma 146:216–223. https://doi.org/10.1016/j.geoderma.2008.05.022
Barré P, Fernandez-Ugalde O, Virto I, Velde B, Chenu C (2014) Impact of phyllosilicate mineralogy on organic carbon stabilization in soils: incomplete knowledge and exciting prospects. Geoderma 235:382–395. https://doi.org/10.1016/j.geoderma.2014.07.029
Bhattacharyya T, Pal DK, Deshpande SB (1993) Genesis and transformation of minerals in the formation of red (Alfisols) and black (Inceptisols and Vertisols) soils on Deccan basalt in the Western Ghats, India. J Soil Sci 44:159–171. https://doi.org/10.1111/j.1365-2389.1993.tb00442.x
Bongiorno G, Bünemann EK, Oguejiofor CU, Meier J, Gort G, Comans R, Mäder P, Brussaard L, de Goede R (2019) Sensitivity of labile carbon fractions to tillage and organic matter management and their potential as comprehensive soil quality indicators across pedoclimatic conditions in Europe. Ecol Indic 99:38–50. https://doi.org/10.1016/j.ecolind.2018.12.008
Bray RH, Kurtz LT (1945) Determination of total, organic, and available forms of phosphorus in soils. Soil Sci 59:39–46
Cambardella CA, Elliott ET (1992) Particulate organic matter changes across a grassland cultivation sequence. Soil Sci Soc Am J 56:777–783. https://doi.org/10.2136/sssaj1992.03615995005600030017x
Carter DL, Heiman MD, Gonzalez CL (1965) Ethylene glycol monoethyl ether for determining surface area of silicate minerals. Soil Sci 100:356–360
Chatterjee D, Datta SC, Manjaiah KM (2013) Clay carbon pools and their relationship with short-range order minerals: avenues to mitigate climate change. Curr Sci 105:1404–1410
Chen X, Wang X, Liebman M, Cavigelli M, Wander M (2014) Influence of residue and nitrogen fertilizer additions on carbon mineralization in soils with different texture and cropping histories. PLoS One 9:e103720. https://doi.org/10.1371/journal.pone.0103720
Churchman GJ, Lowe DJ (2012) Alteration, formation, and occurence of minerals in soils. In: Huang, P., Li, Y., Sumner, M.E. (Eds.), Handbook of Soil Sciences: Properties and Processes, second ed. CRC Press, USA.
Conant RT, Drijber RA, Haddix ML, Parton WJ, Paul EA, Plante AF, Six J, Steinweg JM (2008) Sensitivity of organic matter decomposition to warming varies with its quality. Glob Chang Biol 14:868–877. https://doi.org/10.1111/j.1365-2486.2008.01541.x
Cotrufo MF, Soong JL, Horton AJ, Campbell EE, Haddix ML, Wall DH, Parton WJ (2015) Formation of soil organic matter via biochemical and physical pathways of litter mass loss. Nat Geosci 8:776–779
Dan WA, Nianpeng HE, Qing WA, Yuliang LÜ, Qiufeng WA, Zhiwei XU, Jianxing ZH (2016) Effects of temperature and moisture on soil organic matter decomposition along elevation gradients on the Changbai Mountains, Northeast China. Pedosphere 26:399–407. https://doi.org/10.1016/S1002-0160(15)60052-2
Das D, Nayak AK, Thilagam VK, Chatterjee D, Shahid M, Tripathi R, Mohanty S, Kumar A, Lal B, Gautam P, Panda BB (2018) Measuring potassium fractions is not sufficient to assess the long-term impact of fertilization and manuring on soil’s potassium supplying capacity. J Soils Sediments 18:1806–1820. https://doi.org/10.1007/s11368-018-1922-6
Das R, Purakayastha TJ, Das D, Ahmed N, Kumar R, Biswas S, Walia SS, Singh R, Shukla VK, Yadava MS, Ravisankar N (2019) Long-term fertilization and manuring with different organics alter stability of carbon in colloidal organo-mineral fraction in soils of varying clay mineralogy. Sci Total Environ 684:682–693. https://doi.org/10.1016/j.scitotenv.2019.05.327
Das D, Dwivedi BS, Datta SP, Datta SC, Meena MC, Agarwal BK, Shahi DK, Singh M, Chakraborty D, Jaggi S (2019) Potassium supplying capacity of a red soil from eastern India after forty-two years of continuous cropping and fertilization. Geoderma 341:76–92. https://doi.org/10.1016/j.geoderma.2019.01.041
Das R, Purakayastha TJ, Das D, Ahmed N, Kumar R, Walia SS, Singh R, Shukla VK, Yadava MS, Ravisankar N, Datta SC (2022) Effect of chemical pre-treatment for identification of clay minerals in four soil orders by X-ray diffraction technique. National Acad Sci Lett 45:39–44. https://doi.org/10.1007/s40009-021-01077-4
Datta SC, Takkar PN, Kiran Verma U (2015) Assessing stability of humus in soils from continuous rice-wheat and maize-wheat cropping systems using kinetics of humus desorption. Commun Soil Sci Plant Anal 46:2888–2900. https://doi.org/10.1080/00103624.2015.1104334
Dhaliwal SS, Naresh RK, Gupta RK, Panwar AS, Mahajan NC, Singh R, Mandal A (2020) Effect of tillage and straw return on carbon footprints, soil organic carbon fractions and soil microbial community in different textured soils under rice–wheat rotation: a review. Rev Environ SciBiotechnol 19:103–115. https://doi.org/10.1007/s11157-019-09520-1
Dontsova KM, Bigham JM (2005) Anionic polysaccharide sorption by clay minerals. Soil Sci Soc Am J 69:1026–1035. https://doi.org/10.2136/sssaj2004.0203
Feng X, Simpson AJ, Simpson MJ (2005) Chemical and mineralogical controls on humic acid sorption to clay mineral surfaces. Org Geochem 36:1553–1566. https://doi.org/10.1016/j.orggeochem.2005.06.008
Feng W, Plante AF, Six J (2013) Improving estimates of maximal organic carbon stabilization by fine soil particles. Biogeochemistry 112:81–93. https://doi.org/10.1007/s10533-011-9679-7
Gjems O (1967) Studies on clay minerals and clay-mineral formation in soil profiles in Scandinavia. Med nor Skogforksvesen 21:303–415
Grim RE (1968) Clay mineralogy, 2nd edn. McGraw-Hill Book Co., Inc, New York
Gupta GP, Tembhare BB, Mishra SR (1999) Characterization and classification of soils of granitic terrain in Jabalpur district of Madhya Pradesh. Agropedol 9:77–81
Hassink J (1994) Effects of soil texture and grassland management on soil organic C and N and rates of C and N mineralization. Soil BiolBiochem 26:1221–1231. https://doi.org/10.1016/0038-0717(94)90147-3
Haynes RJ (2005) Labile organic matter fractions as central components of the quality of agricultural soils: an over view. AdvAgron 5:221–268
Jackson, ML (1985) Soil chemical analysis-advanced course. 11th printing published by the author. Madison WI
Jones DL, Willett VB (2006) Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil. Soil BiolBiochem 38:991–999. https://doi.org/10.1016/j.soilbio.2005.08.012
Kahle M, Kleber M, Jahn R (2003) Retention of dissolved organic matter by illitic soils and clay fractions: influence of mineral phase properties. J Plant Nutr Soil Sci 166:737–741. https://doi.org/10.1002/jpln.200321125
Kaiser K, Guggenberger G (2003) Mineral surfaces and soil organic matter. Euro J Soil Sci 54:219–236. https://doi.org/10.1046/j.1365-2389.2003.00544.x
Kalbitz K, Schwesig D, Rethemeyer J, Matzner E (2005) Stabilization of dissolved organic matter by sorption to the mineral soil. Soil BiolBiochem 37:1319–1331. https://doi.org/10.1016/j.soilbio.2004.11.028
Kassambara A, Mundt F (2017) Factoextra: extract and visualize the results of multivariate data analyses. R Package Version 1:337–354
Kettler TA, Doran JW, Gilbert TL (2001) Simplified method for soil particle-size determination to accompany soil-quality analyses. Soil Sci Soc Am J 65(3):849–852. https://doi.org/10.2136/sssaj2001.653849x
Kothawala DN, Moore TR, Hendershot WH (2009) Soil properties controlling the adsorption of dissolved organic carbon to mineral soils. Soil Sci Soc Am J 73:1831–1842
Lal R (2007) Carbon management in agricultural soils. Mitig Adapt Strateg Glob Chang 12:303–322. https://doi.org/10.1007/s11027-006-9036-7
Lal R (2008) Sequestration of atmospheric CO 2 in global carbon pools. Energy Environ Sci 1:86–100. https://doi.org/10.1039/B809492F
Larionova AA, Maltseva AN, Lopes de Gerenyu VO, Kvitkina AK, BykhovetsSS ZBN, Kudeyarov VN (2017) Effect of temperature and moisture on the mineralization and humification of leaf litter in a model incubation experiment. Eurasian Soil Sci 50(4):422–431. https://doi.org/10.1134/S1064229317020089
Lê S, Josse J, Husson F (2008) FactoMineR: an R package for multivariate analysis. J Stat Softw 25:1–18. https://doi.org/10.18637/jss.v070.i01
Leinweber P, Schulten HR, Jancke H (1999) New evidence for the molecular composition of soil organic matter in Vertisols. Soil Sci 164:857–870
Mayes MA, Heal KR, Brandt CC, Phillips JR, Jardine PM (2012) Relation between soil order and sorption of dissolved organic carbon in temperate subsoils. Soil Sci Soc Am J 76:1027–1037. https://doi.org/10.2136/sssaj2011.0340
Moore DM, Reynolds Jr RC (1989) X-ray diffraction and the identification and analysis of clay minerals. Oxford University Press (OUP).
Nguyen TT, Marschner P (2014) Retention and loss of water extractable carbon in soils: effect of clay properties. Sci Total Environ 470:400–406. https://doi.org/10.1016/j.scitotenv.2013.10.002
Olivoto T, Lúcio ADC (2020) metan: an R package for multi-environment trial analysis. Methods EcolEvol 11:783–789. https://doi.org/10.1111/2041-210X.13384
Olsen SR, Cole CL, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA Circular No. 939, Washington, 72–75.
Page AL, Miller RH, Keeney DR (1982) Methods of soil analysis. Part 2. Chemical and microbiological properties. Agronomy, No. 9. Soil Science Society of America, Madison, WI, 1159.
Pal DK (2003) Significance of clays, clay and other minerals in the formation and management of Indian soils. J Indian Soc Soil Sci 51:338–364
Pal DK, Srivastava P, Bhattacharyya T (2003) Clay illuviation in calcareous soils of the semiarid part of the Indo-Gangetic Plains, India. Geoderma 115:177–192. https://doi.org/10.1016/S0016-7061(02)00377-4
Pal DK (2017) A treatise of Indian and tropical soils (p. 9). Cham: Springer International Publishing https://doi.org/10.1007/978-3-319-49439-5_2
Peel MC, Finlayson BL, McMahon TA (2007) Updated world map of the Köppen-Geiger climate classification. Hydrol Earth Syst Sci 11:1633–1644. https://doi.org/10.5194/hess-11-1633-2007
Piazza G, Pellegrino E, Moscatelli MC, Ercoli L (2020) Long-term conservation tillage and nitrogen fertilization effects on soil aggregate distribution, nutrient stocks and enzymatic activities in bulk soil and occluded microaggregates. Soil till Res 196:104482. https://doi.org/10.1016/j.still.2019.104482
Pillai M, Pal DK, Deshpande SB (1996) Distribution of clay minerals and their genesis in ferruginous and black soils occurring in close proximity on Deccan basalt plateau of Nagpur district, Maharashtra. J Indian Soc Soil Sci 44:500–507
Rashad M, Dultz S, Guggenberger G (2010) Dissolved organic matter release and retention in an alkaline soil fromthe Nile River Delta in relation to surface charge and electrolyte type. Geoderma 158:385–391. https://doi.org/10.1016/j.geoderma.2010.06.007
Roychand P, Marschner P (2013) Respiration in a sand amended with clay–effect of residue type and rate. Eur J Soil Biol 58:19–23. https://doi.org/10.1016/j.ejsobi.2013.05.005
Rudrappa L, Purakayastha TJ, Singh D, Bhadraray S (2006) Long-term manuring and fertilization effects on soil organic carbon pools in a TypicHaplustept of semi-arid sub-tropical India. Soil till Res 88:180–192. https://doi.org/10.1016/j.still.2005.05.008
Saggar S, Parshotam A, Sparling GP, Feltham CW, Hart PB (1996) 14C-labelled ryegrass turnover and residence times in soils varying in clay content and mineralogy. Soil BiolBiochem 28:1677–1686. https://doi.org/10.1016/S0038-0717(96)00250-7
Saggar S, Parshotam A, Hedley C, Salt G (1999) 14C-labelled glucose turnover in New Zealand soils. Soil BiolBiochem 31:2025–2037. https://doi.org/10.1016/S0038-0717(99)00126-1
Saidy AR, Smernik RJ, Baldock JA, Kaiser K, Sanderman J, Macdonald LM (2012) Effects of clay mineralogy and hydrous iron oxides on labile organic carbon stabilisation. Geoderma 173:104–110. https://doi.org/10.1016/j.geoderma.2011.12.030
Saidy AR, Smernik RJ, Baldock JA, Kaiser K, Sanderman J (2013) The sorption of organic carbon onto differing clay minerals in the presence and absence of hydrous iron oxide. Geoderma 209:15–21. https://doi.org/10.1016/j.geoderma.2013.05.026
Samra JS (1982) Clay mineralogical studies in relation to soil development and vegetation in Haldwani forest division of Uttar Pradesh. Indian forester.
Sarkar B, Singh M, Mandal S, Churchman GJ, Bolan NS (2018) Clay minerals—organic matter interactions in relation to carbon stabilization in soils. In The future of soil carbon (pp. 71–86). Academic Press. https://doi.org/10.1016/B978-0-12-811687-6.00003-1
Schmidt MW, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DA, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011) Persistence of soil organic matter as an ecosystem property. Nature 478:49–56
Schweizer SA, Mueller CW, Höschen C, Ivanov P, Kögel-Knabner I (2021) The role of clay content and mineral surface area for soil organic carbon storage in an arable toposequence. Biogeochem 156:401–420. https://doi.org/10.1007/s10533-021-00850-3
Sharma SD (1982) Status of organic matter in some typical soils of Doon valley with special reference to regeneration and quality of forests (Doctoral dissertation, Ph. D. Thesis, HNB Garwhal University Srinagar (Uttarakhand), India).
Singh M, Sarkar B, Biswas B, Churchman J, Bolan NS (2016) Adsorption-desorption behavior of dissolved organic carbon by soil clay fractions of varying mineralogy. Geoderma 280:47–56. https://doi.org/10.1016/j.geoderma.2016.06.005
Singh M, Sarkar B, Biswas B, Bolan NS, Churchman GJ (2017) Relationship between soil clay mineralogy and carbon protection capacity as influenced by temperature and moisture. Soil BiolBiochem 1:95–106. https://doi.org/10.1016/j.soilbio.2017.02.003
Singh M, Sarkar B, Sarkar S, Churchman J, Bolan N, Mandal S, Menon M, Purakayastha TJ, Beerling DJ (2018) Stabilization of soil organic carbon as influenced by clay mineralogy. AdvAgron 148:33–84. https://doi.org/10.1016/bs.agron.2017.11.001
Singhal RM, Sharma SD (1983) Study of organic matter of some typical soils of Doon valley forests. Indian journal of forestry.
Six J, Elliott ET, Paustian K, Doran JW (1998) Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci Soc Am J 62:1367–1377. https://doi.org/10.2136/sssaj1998.03615995006200050032x
Six J, Conant RT, Paul EA, Paustian K (2002) Stabilization mechanisms of soil organic matter: implications for C-saturation of soils. Plant Soil 241:155–176. https://doi.org/10.1023/A:1016125726789
Snyder JD, Trofymow JA (1984) Rapid accurate wet oxidation diffusion procedure for determining organic and inorganic carbon in plant and soil samples. Commun Soil Sci Plant Anal 15:1587–1597. https://doi.org/10.1080/00103628409367499
Souza GP, Figueiredo CC, Sousa DM (2016) Relationships between labile soil organic carbon fractions under different soil management systems. Sci Agric 73:535–542. https://doi.org/10.1590/0103-9016-2015-0047
Steffens M, Rogge DM, Mueller CW, Höschen C, Lugmeier J, Kölbl A, Kögel-Knabner I (2017) Identification of distinct functional microstructural domains controlling C storage in soil. Environ SciTechnol 51:12182–12189. https://doi.org/10.1021/acs.est.7b03715
Subbiah BV, Asija GL (1956) A rapid procedure for the estimation of available nitrogen in soils. CurrSci 25:259–260
Surya JN, Walia CS, Ahmad N, Singh H, Goyal V, Khajuria V (2015) Characterization and clay minerals composition of soils derived from metamorphic formation of Kumaon Himalayas. Clay Res 34:15–24
Suseela V, Conant RT, Wallenstein MD, Dukes JS (2012) Effects of soil moisture on the temperature sensitivity of heterotrophic respiration vary seasonally in an old-field climate change experiment. Glob Chang Biol 18:336–348. https://doi.org/10.1111/j.1365-2486.2011.02516.x
Tisdall J. (1996) Formation of soil aggregates and accumulation of soil organic matter. In: Carter, M.R., Stewart, B.A. (Eds.), Structure and Organic Matter Storage in Agricultural Soils. CRC press 57–96 https://doi.org/10.1201/9781003075561-5
Ugochukwu UC (2017) Measurement of surface area of modified clays by ethylene glycol monoethyl ether method. Chem Asian J 29:1891–1896
Walkley AJ, Black CA (1934) Estimation of soil organic carbon by the chromic acid titration method. Soil Sci 37:29–38
Wattel-Koekkoek EJ, Van Genuchten PP, Buurman P, Van Lagen B (2001) Amount and composition of clay-associated soil organic matter in a range of kaolinitic and smectitic soils. Geoderma 99:27–49. https://doi.org/10.1016/S0016-7061(00)00062-8
Weil RR, Islam KR, Stine MA, Gruver JB, Samson-Liebig SE (2003) Estimating active carbon for soil quality assessment: a simplified method for laboratory and field use. Am J Altern Agric 18:3–17. https://doi.org/10.1079/AJAA200228
Wright SF, Starr JL, Paltineanu IC (1999) Changes in aggregate stability and concentration of glomalin during tillage management transition. Soil Sci Soc Am J 63:1825–1829. https://doi.org/10.2136/sssaj1999.6361825x
Acknowledgements
The authors extend their deep appreciation to the Indian Council of Agricultural Research (ICAR), New Delhi and Ministry of Agriculture and Farmers Welfare, Government of India, New Delhi, for providing the technical and financial support.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Das, A., Purakayastha, T.J., Ahmed, N. et al. Influence of Clay Mineralogy on Soil Organic Carbon Stabilization under Tropical Climate, India. J Soil Sci Plant Nutr 23, 1003–1018 (2023). https://doi.org/10.1007/s42729-022-01099-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42729-022-01099-x