Abstract
Immunoglobulin (Ig)M nephropathy (IgMN), known since 1978, is a very controversial clinicopathological entity characterized by IgM diffuse deposits in the mesangium at immunofluorescence whereas light microscop identifies minimal glomerular lesion, hypercellularity and expansion of the mesangium or sclerotic focal, segmental lesion. Clinically, it is a nephrotic syndrome, especially in pediatric patients, or asymptomatic proteinuria and/or isolated hematuria. These characteristics narrowly define IgMN between minimal change disease and focal segmental glomerulosclerosis, so it is not often recognized as a separate pathology. Homogeneous epidemiologic, pathogenetic, clinical or histological data are not available. Recent research on the pathogenetic role of mesangial IgM has, however, renewed interest in IgMN and naturally the controversies.
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Introduction
Immunoglobulin (Ig)M nephropathy (IgMN) is a clinicopathological entity characterized by dominant diffuse and granular IgM deposits within the mesangium at immunofluorescence (IF), and the histological picture varies from no glomerular abnormalities to proliferation of mesangial cells, accumulation of extracellular mesangial matrix and segmental or global sclerosis. IgMN was first described in 1978 by Cohen et al. and Bhasin et al. [1, 2] as a distinct entity, but thus far there is no consensus about the significance of the presence of IgM deposits in the context of minimal change disease (MCD), mesangial proliferative glomerulonephritis (MePGN), and focal segmental glomerulosclerosis (FSGS), without associated systemic disorders [3–5]. The interpretation of IF findings is controversial in that some authors have described cases with IgM trace positivity on IF as IgMN while others have described cases with 1+ or 2+ IgM deposits as MCD. The significance of IgMN in the range of idiopathic MCD or FSGS glomerular disease and the epidemiology of IgMN are controversial. The frequency of IgMN in native renal biopsy series in children or adults varies from 2 to 18.5 % [6–8]. The disease is more common in children than in adults. In Italy, the annual frequency of IgMN over the period 1987–1993 was 1.9 % [9], but in the Italian Registry of Renal Biopsies (IRRB) from 1996 to 2000 [10] MCD, FSGS and MePGN without IgA deposits were considered as primary glomerulonephritis. The greatest prevalence of IgMN was reported in Thailand (45.8 % of 2154 biopsies) with a decline in frequency to 16.9 % from 2003–2005 [11]. We believe this demonstrates that there was an initial interest in IgMN in the 1980s, while differences in policy of renal biopsy practice in different countries makes it difficult to compare the databases across countries. The correlation with varying biopsy timing and indications or genetic or environmental factors has still to be clarified. The clinical characteristics of IgMN do not differ from MCD and FSGS since they are associated with nephrotic syndrome (NS), steroid-resistant nephrotic syndrome (SRNS), hematuria and/or asymptomatic proteinuria. These disorders may have similar immunopathological features but are distinguished in the clinical course, in the response to therapy and the progression to end-stage renal disease (ESRD). Are MCD, IgMN and FSGS separate entities or are they part of the same spectrum of disease with overlapping features? Is IgMN in children with minimal change nephrotic syndrome a marker of disease severity, useful for a more aggressive therapeutic approach, and a prognostic marker? To answer these questions we will consider the problem from different points of view.
The morphological point of view
IgMN to date is still far from being determined based on strict pathological criteria. In his review, published in 1988, Border claimed that MePGN, MCD and idiopathic focal sclerosis are three separate disorders but they overlap because the morphological alterations of MCD appear similar to the minimal changes found in early MePGN and focal sclerosis is similar to the findings in the late stage of MePGN [12]. According to Border, the central role of the mesangium is where the immune complex is localized and the earliest histologic lesion may be segmental mesangial proliferation, which can progress to generalized mesangial hypercellularity with matrix expansion, and ultimately to segmental or diffuse sclerosis [13–15]. The distinction between the three entities is based on IF with high inter-observer variability. The light microscopy (LM) and electron microscopy (EM) pictures can be identical to that of MCD or characterized by FSGS. In several series, the diagnosis of IgMN required examination of renal biopsy by LM, IF, and EM. Some investigators consider this entity as a transitional form between MCD and FSGS [16, 17]. The International Study of Kidney Disease in Children (ISKDC) did not differentiate MCD with IgM-positive immunofluorescence from IgM-negative MCD in childhood with nephrotic syndrome [18].
Immunofluorescence
IgMN is characterized by the presence of granular, diffuse and global IgM deposits in the glomerular mesangium. However, in MCD the IgM intensity is always minimal while in FSGS IgM is segmentally and focally distributed in sclerosis areas, not diffuse, as in IgMN [7, 19, 20]. Complementary fragments of C3 are found in the majority of cases, co-localized with IgM deposits of variable intensity [1, 8, 21].
Light microscopy
The spectrum of LM findings is quite heterogeneous. The morphologic alterations range from minor changes, to variable degrees of mesangial proliferation (usually of mild to moderate degree), to an FSGS pattern accompanied by adhesion formation with the Bowman’s capsule [1, 4, 20, 22]. Some authors have also reported cellular crescents [23, 24]. It is difficult to distinguish an MCD pattern on the basis of LM examination alone, and IF and EM were required to describe the picture reported in numerous series [7, 8, 17, 25–28]. An FSGS pattern as the morphologic expression of IgMN is the most controversial feature. Many studies have excluded cases with this morphology from the IgMN category, while others have observed this lesion in a significant number of cases characterized by global IgM mesangial positivity in contrast to nonspecific, segmental IgM trapping in the idiopathic form of FSGS. The reports on prevalence of this morphologic pattern in biopsies of IgMN vary widely from 9 to 65.2 % of cases [20]. Focal global sclerosis is also quite common but it can be missed due to biopsy sampling error. Some researchers have noted progression of IgMN with minor changes or mesangial proliferation to FSGS on repeated biopsies in a variety of cases [19, 20, 25, 28]. Tubulointerstitial lesions such as tubular atrophy and interstitial scarring are also commonly observed at diagnosis and are usually mild [8, 19–21].
Electron microscopy
There are very few studies on the ultrastructural features of IgMN [20]. In the majority of cases, no EM was done and the diagnosis was made on the basis of IF microscopy. The few studies that have carried out EM examination have noted small, granular to short linear electron-dense deposits in the mesangium and paramesangium, along with variable degrees of mesangial cell proliferation and mesangial matrix expansion. Variable degrees of podocyte foot effacement, segmental or diffuse, have also been observed [20, 21, 25, 26, 28]. To confirm the difficulty of defining pathological criteria, we report in Tables 1 and 2 the most frequent biopsy findings from the most relevant series in the English-language international literature between 1991 and 2014.
The clinical point of view
In general, clinical manifestations of IgMN are variable and the commonest presentation in the pediatric population is NS; nephropathy can occur, also, in young adults and at any age presenting as steroid-resistant nephrotic syndrome, proteinuria and/or hematuria. The natural history and the prognosis of IgMN, like its presentation and morphology, are also quite varied; probably this is in part due to the variable length of follow-up and differences in the classification criteria across studies. There are only a few long-term longitudinal studies on IgMN and the majority are retrospective. In most studies, the number of patients was relatively small and the follow-up period short. Corticosteroids remain the mainstay of therapeutic strategies in these patients, as in MCD or primary FSGS [12]. The steroid response varies considerably across the studies. Many reports have demonstrated increased steroid resistance and a less-favorable outcome in IgMN compared to MCD and, thus, they consider it as a distinct clinicopathological entity [12, 19, 20, 25, 29, 30]. In 1983, Tejani and coworkers compared two groups of nephrotic patients, both with minimal changes in association or not with IgM deposits at LM. The patients with IgM deposits were older and 60 % were steroid-dependent compared to only 14 % of those without IgM [31]. In 2003, Myllymaki et al. in Finland published one of the largest and longest follow-up studies including 110 pediatric and adult patients with IgMN and NS [19]. During a 15-year follow-up, 36 % of patients developed renal insufficiency and 23 % reached ESRD. Hypertension was diagnosed in 50 % of patients, and at multivariate analysis was the only significant risk factor for renal failure. Among histological parameters, interstitial fibrosis had the strongest prognostic value. Twenty-nine percent of nephrotic patients were resistant to corticosteroids whereas 80 % of patients with steroid-sensitive disease were steroid-dependent. In this study, 5/11 repeated biopsies for NS showed a typical histopathologic pattern of FSGS [19]. There is controversy, however, in the literature about the clinicopathological correlation and outcome. Prasad et al. and Al-Eisa et al. concluded that MCD and IgMN are clinically indistinguishable in children who are biopsied for NS [32, 33] while Zeis et al. and Swartz et al. showed a worse response to therapy in IgMN, suggesting IgM positivity at IF as a surrogate marker for severity of MCD [25, 34].
In Table 3 we report the main clinical presentation observed in various studies, with the different renal biopsy criteria, comprising, most frequently, idiopathic nephrotic syndrome and, rarely, isolated hematuria and/or proteinuria. Available clinical data at follow-up are reported in Table 4.
There are few data available on the use and response rate of immunosuppressive agents in patients with IgMN. Oral cyclophosphamide has been used in a small number of steroid-resistant or steroid-dependent patients with a response rate of up 50 % [20]. Kanemoto et al. in 2013 reviewed the clinical course of 70 children with steroid-dependent or steroid-resistant NS and observed that 30 IgM positive and/or C1q positive NS had good responses to cyclosporine, regardless of the histological pattern [26]. Also Spreitzer et al. in 2014 concluded that IgM IF-positive MCD did not signify a worse prognosis in children in comparison to C1q IF-positive MCD or IF-negative MCD [28]. Moreover, the proportion of patients in their study who received adjuvant immunosuppressive therapy (cyclophosphamide or levamisole or cyclosporine or mycophenolate mofetil) and the median of relapse-free survival time after initiation did not differ statistically between groups [28].
In contrast to FSGF, which is well known to recur early after a renal graft, there are only a few cases describing the recurrence of IgMN after transplantation [35–37]. In all cases, renal failure progressed to ESRD within several years after the diagnosis of NS, and the patients were steroid- dependent or -resistant. Rituximab was effective in two cases in combination with plasma exchange and immunoglobulins [36, 37].
The pathogenetic point of view
Since the first clinicopathological studies on MCD and FSGS in the 1970s, new insights derived from both animal models and genetic studies have enabled a better definition of these diseases, above all the identification of podocytes as the major cellular target. Injury of different kinds to the podocytes leads to a loss of interdigitating foot processes along the outer aspect of the glomerular capillary wall and podocyte depletion through detachment, apoptosis or necrosis and disruption of the glomerular charge-selective sieving barrier, leading to proteinuria [38, 39]. Clinical and experimental studies have suggested that MCD is an immune-mediated disease in which podocytes are affected by circulating cytokines produced by peripheral blood mononuclear cells or, more specifically, T-cells, pointing to a role of the immune system [40]. However, the molecular basis for MCD is still uncertain. FSGS is a pattern of injury, divided into forms based on: (1) gene disruption involving nephrin, podocin as cell-adhesion and cell-signaling proteins, and (2) extrinsic or systemic factors including viral infection, toxicity, complement activation, intracapillary hypertension, and metabolic storage diseases [39]. Studies on the biology of glomerular visceral epithelial cells have revealed the pathophysiology of cell–cell and cell–matrix interaction governing the integrity of the cell and the glomerular capillary filter and its malfunction in the nephrotic syndrome in MCD and FSGS [38, 39]. In patients with IgMN, some studies have found elevated serum IgM or IgM immune complex concentrations without structural or biochemical abnormalities of the IgM molecule, as observed in IgA nephropathy [17, 20]. The immune complex-mediated activation of the classical complement pathway has been suggested by observation of the co-localization of the complementary components along with IgM in the glomerular mesangium, in particular C3 [17, 20]. Strassheim et al. in 2013 hypothesized that IgM, as a “natural antibody”, binds to endogenous neopitopes of glomerular cells after injury, as in other tissues, activating the complement system. The authors, in an experimental model of mice with adriamycin nephropathy, showed that the depletion of B cells prevented deposition of IgM and C3 in glomeruli reducing proteinuria and glomerulosclerosis and suggesting that IgM and C3 might be pathogenic and not only markers of immune injury. Also, in a subset of patients diagnosed with primary FSGS, the same authors showed that IgM and C3d co-localization indicate that IgM binds to specific glomerular epitopes and complement activation [41]; measurement of complement activation fragments in plasma from patients with FSGS further demonstrates that the complement system is activated in this disease [42]. Panzer et al., in the same group of researchers, in 2015 studied whether IgM can add to existing cellular damage, possibly by activating complement via the classical pathway (involving C1q, C4, and C2). They used an elegant murine model of non sclerotic glomerular disease. They argue that it is possible that glomerular injury simultaneously increases the classical pathway activation by natural IgM, which binds to injury-associated epitopes while also decreasing the ability of complement regulatory proteins within the glomerulus to control amplification of complement activation through the alternative pathway. Binding of IgM within the glomerulus can be a downstream event occurring secondary to glomerular damage as part of the repair process to help remove apoptic cells but it may also result in local tissue damage [43].
Discussion
The relationship between MCD, IgMN and FSGS remains controversial. Some researchers have proposed that IgMN is a transitional state between MCD and FSGS while others have suggested that in the course of time IgMN may convert to FSGS [15, 25, 31]. These hypotheses are sustained by the low rate of response to therapy compared to MCD, the slow progression of renal failure and the low rate of recurrence post-transplantation compared to FSGS. Because patients with FSGS have a poor prognosis it is important to identify the clinical or pathological factors that predict the progression of IgMN to FSGS. It is difficult to obtain a homogeneous patient group: at the time of presentation, the duration of proteinuria varies and some patients may have already started treatment. Recently, there has been an increase in the number of publications on this disease and a renewed interest in this area to try and understand if IgM has any pathogenic role and, if so, what. Panzer and colleagues [40] studied an animal experimental model suggesting that IgM and C3 might be pathogenic and not markers of nonimmune injury: IgM bound to damaged glomeruli activated complement and amplified injury. The recent shift of attention to the pathogenic role of IgM leads to thinking that only with prospective studies and a general consensus regarding the pathological classification will it be possible to understand the etiology and pathogenesis of the disease.
In conclusion, it seems possible to describe IgMN from a clinical and morphological perspective as ranging from MCD to FSGS, but it is important to look at the immunopathogenic role of IgM to identify the subset of nephrotic patients in whom these molecular mechanisms are involved and so likely to benefit from treatment with newer drugs that target B cells or the complement system.
References
Cohen AH, Border WA, Glassock RJ (1978) Nephrotic syndrome with glomerular mesangial IgM deposits. Lab Invest 38:610–619
Bhasin HK, Abuelo JG, Nayak R, Esperaza AR (1978) Mesangial proliferative glomerulonephritis. Lab Invest 39:21–29
Vangelista A, Frasca G, Biagini G, Bonomini V (1981) Long term study of mesangial proliferative glomerulonephritis with IgM deposits. Proc Eur Dial Transpl Assoc 18:503–507
Cavallo T, Johnson MP (1981) Immunopathologic study of minimal change glomerular disease with mesangial IgM deposits. Nephron 27(6):281–284
Cohen AH, Border WA (1982) Mesangial proliferative glomerulonephritis. Semin Nephrol 2:228–240
Vanikar A (2013) IgM nephropathy; can we still ignore it. J Nephropathol 2(2):98–103
Mubarak M, Kazi JI, Shakeel S, Lanewala A, Hashmi S, Akhter F (2011) Clinicopathologic characteristics and steroid response of IgM nephropathy in children presenting with idiopathic nephrotic syndrome. APMIS 119:180–186
Singhai AM, Vanikar AV, Goplani KR, Kanodia KV, Patel RD, Suthar KS et al (2011) Immunoglobulin M nephropathy nephropathy in adults and adolescents in India: a single-center study of natural history. Indian J Pathol Microbiol 54(1):3–6
Schena FP (1997) Survey of the Italian Registry of Renal Biopsies. Frequency of the renal diseases for 7 consecutive years. The Italian Group of Renal Immunopathology. Nephrol Dial Transplant 12:418–426
Gesualdo L, Di Palma AM, Morrone LF, Strippoli GF, Schena FP, Italian Immunopathology Group, Italian Society of Nephrology (2004) The Italian experience of the national registry of renal biopsies. Kidney Int 66(3):890–894
Parichatikanond P, Chawanasuntorapoj R et al (2006) An Analysis of 3555 cases of renal biopsy inThailand. J Med Assoc Thai 89(Suppl. 2):S106–S111
Border WA (1988) Distinguishing minimal-change disease from mesangial disorder. Kidney Int 34:419–434
Kashgarian M (1985) Mesangium and glomerular disease. Lab Invest 52:569–571
Vilches AR, Turner DR, Cameron JS, Ogg CS, Clantler C, Williams DG (1982) Significance of mesangial IgM deposition in minimal change nephrotic syndrome. Lab Invest 46:10–15
Ji-Yun Y, Melvin T, Sibley R, Michael AP (1984) No evidence for a specific role of IgM in mesangial proliferation of idiopathic nephrotic syndrome. Kidney Int 25:100–106
Fogo AB (2001) Minimal change disease and focal segmental glomerulosclerosis. Nephrol Dial Transplant 16:74–76
Hsu HC, Chen WY, Lin GJ, Chen L, Kao SL, Huang CC, Lin CY (1984) Clinical and immunopathologic study of mesangial IgM nephropathy: report of 41 cases. Histopathology 8:435–446
International Study of kidney disease in Children (1974) Prospective, controlled trial of cyclophosphamide therapy in children with nephrotic syndrome. Lancet 2:423–427
Myllymäki J, Saha H, Mustonen J, Helin H, Pasternack (2003) IgM nephropathy: clinical picture and long-term prognosis. Am J Kidney Dis 41(2):343–350
Mubarak M, Kazi JI (2012) IgM nephropathy revisited. Nephro-Urol Mon 4:603–608
Mokhtar GA (2011) IgM nephropathy: clinical picture and pathological findings in 36 patients. Saudi J Kidney Dis Transpl 22:969–975
Saha H, Mustonen J, Pastermack A, Helin H (1989) Clinical follow-up of 54 patients with IgM nephropathy. Am J Nephrol 9:124–128
O’Donoghue DJ, Lawler W, Hunt LP, Acheson EJ, Mallick NP (1991) IgM associated primary diffuse mesangial proliferative glomerulonephritis: natural history and prognostic indicators. Q J Med 79(288):333–350
Kazi J, Mubarak M (2014) IgM nephropathy presenting as full blown crescentic glomerulonephritis: first report in the literature. Nefrologia 34(3):423–424
Zeis PM, Kavazarakis E, Nakopoulou L, Moustaki M, Messaritaki A, Zeis MP et al (2001) Glomerulopathy with mesangial IgM deposits: long-term follow up of 64 children. Pediatr Int 43(3):287–292
Kanemoto K, Ito H, Anzai M, Matsumura C, Kurayama H (2013) Clinical significance of IgM and C1q deposition in the mesangium in pediatric idiopathic nephrotic syndrome. J Nephrol 26(2):306–314
Vanikar AV, Kanodia KV, Patel RD, Suthar KS, Patel HV, Gumber MR, Trivedi HL (2012) IgM Nephropathy in India: a single centre experience. Indian J Pediatr 79(8):1025–1027
Spreitzer MV, Vizjak A, Ferluga D, Kenda RB, Kersnik Levart T (2014) Do C1q or IgM nephropathies predict disease severity in children with minimal change nephrotic syndrome? Pediatr Nephrol 29(1):67–74
Kopolovic J, Shvil Y, Pomeranz A, Ron N, Rubinger D, Oren R (1987) IgM nephropathy: morphological study related to clinical findings. Am J Nephrol 7:275–280
Mubarak M, Naqvi R, Kazi JI, Shakeel S (2013) Immunoglobulin M nephropathy in adults: a clinicopathological study. Iran J Kidney Dis 7:214–219
Tejani A, Nicastri AD (1983) Mesangial IgM nephropathy. Nephron 35:1–5
Prasad DR, Zimmerman SW, Barkholder PM (1977) Immunohistologic features of minimal change nephrotic syndrome. Arch Pathol Lab Med 101:345–349
Al-Eisa A, Carter JE, Lirenman DS, Magil AB (1996) Childhood IgM nephropathy: comparison with minimal change disease. Nephron 72:37–43
Swartz SJ, Eldin KW, Hicks MJ, Feig DI (2009) Minimal change disease with IgM+ immunofluorescence: a subtype of nephrotic syndrome. Pediatr Nephrol 24:1187
Salmon AHJ, Kamel D, Mathiedon PW (2004) Recurrence of IgM nephropathy in a renal allograft. Nephrol Dial Transplant 19:2650–2652
Westphal S, Hansson S, Mjornsted L, Molne J, Swerkersson S, Friman S (2006) Early recurrence of nephrotic syndrome (immunoglobulin m nephropathy) after renal transplantation successfully treated with combinations of plasma exchanges, immunoglobulin, and rituximab. Transplant proc 38(8):2659–2660
Betjes MG, Roodnat JI (2009) resolution of IgM nephropathy after rituximab treatment. Am J Kidney Dis 53(6):1059–1062
D’Agati VD, Kaskel FJ, Falk Ronald J (2011) Focal Segmental Glomerulosclerosis. N Engl J Med 365:2398–2411
Weening JJ, Ronco P, Remuzzi G (2013) Advances in the pathology of the glomerular diseases. In: Chen N (ed) New Insights into Glomerulonephritis. Contrib Nephrol, vol 181. Karger, Basel, pp 12–21
Den Berg Van, Jg Weening JJ (2004) Role of the immune system in the pathogenesis of idiopathic nephrotic syndrome. Clin Sci 107:125–136
Strassheim D, Renner B, Panzer S, Fuquay R, Kulik L, Ljubanović D, Holers VM, Thurman JM (2013) IgM contributes to glomerular injury in FSGS. J Am Soc Nephrol 24(3):393–406
Thurman JM, Wong M, Renner B, Frazer-Abel A, Giclas PC, Joy MS, Jalal D, Radeva MK, Gassman J, Gipson DS, Kaskel F, Friedman A, Trachtman H (2015) Complement activation in patients with focal segmental glomerulosclerosis. PLoS One 10(9):e0136558
Panzer SE, Laskowski J, Renner B, Kulik L, Ljubanovic D, Huber KM, Zhong W, Pickering MC, Holers VM, Thurman JM (2015) IgM exacerbates glomerular disease progression in complement-induced glomerulopathy. Kidney Int 88:528–537
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Brugnano, R., Del Sordo, R., Covarelli, C. et al. IgM nephropathy: is it closer to minimal change disease or to focal segmental glomerulosclerosis?. J Nephrol 29, 479–486 (2016). https://doi.org/10.1007/s40620-016-0269-6
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DOI: https://doi.org/10.1007/s40620-016-0269-6