Abstract
Sarcocystis spp. are a group of tissue cyst-forming coccidia which infect a vast range of animals as well as human beings. Found frequently in animal carcasses at slaughter, undermining their value, they have also been found associated with clinical disease. Dogs and cats are involved in the transmission. Studies in India point to a vast reservoir of infection with high prevalence rates in various livestock species. However, there is a glaring paucity of reports on the horse and Sarcocystis of the camel has remained totally unexplored so far. At least two different Sarcocystis spp. can parasitize each livestock host species. Experimental transmission studies have provided additional parameters for distinguishing the species. The clinical symptoms are generally non-specific and diagnosis in the living animal, by the presently available means, is almost impossible. Immunodiagnosis till now is beset with problem of cross-reactivity. Treatment with anti-coccidials presently tried do not seem satisfactory. Of the two zoonotic species with cattle-man and pig-man cycles, only the latter seems of some significance in India due to backyard pig-rearing and slaughter practices. It is a paradox that despite high prevalence of S. suihominis in pigs, reports of human cases are limited. This and some of the existing grey areas of information in the Indian context, have been highlighted as also possible directions for future research.
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Introduction
Sarcocystis, originally described from the muscle of the pig, was recognized only as a common parasite in the musculature of herbivores for nearly a century. It was not until 1972 that its obligatory two host life cycle was unravelled and helped to identify it as a group of faecally-transmitted coccidians involving herbivores as intermediate hosts and carnivores as the definitive hosts (Dubey et al. 1989a). Since then, there has been a vast upsurge of interest and accumulation of information. Broadly, it has been found that each of the livestock species has two or more types of Sarcocystis parasitizing them depending on the specific definitive host (carnivores like dog, cat and man) utilized for the completion of its life cycle. Their clinical and zoonotic significance has increasingly emerged in the last three decades. During this period, a large number of studies have been carried out at various centres in India, but reviews on this subject have dealt mainly with epidemiological or zoonotic aspects (Shah 1990; Juyal 1991; Shah 1995). As such, a review aimed at providing a comprehensive update of this important parasitism from an Indian perspective, seems justified.
Epidemiology
Unlike previously known coccidia, Sarcocystis develops gamonts and oocysts in the lamina propria of the carnivore definitive hosts. These oocysts get sporulated in situ and sporocysts are passed in the faeces of these hosts (dog, cat, man). Earlier workers observed the faecal sporocysts in natural infections but identified only up to the level of genus. Sarcocystis was detected as part of coccidia and helminths in faeces of domiciled cats (Chhabra et al. 1984) and dogs (Singh et al. 1987). The latter reported high prevalence in stray dogs which had easy access to the source viz. slaughter house and meat shops. Likewise, Shastri (1989) found an overall sporocyst presence in 67.79 % of 385 dog samples in Parbhani town (Marathwada), higher (76.50 %) from outskirt areas as against only 28.57 % in mainly domiciled dogs from the town’s interior. On the other hand, Mamatha et al. (2006) could detect sporocysts in only two of 140 faecal samples from dogs and none from 100 cats in metropolitan Bangalore. Close association with dogs was also considered a factor in the transmission of Sarcocystis infections in ruminants (Venu and Hafeez 2000). Sarcocystis spp. manifest rigid host specificity for their intermediate hosts as indicated by the failure of cross-transmission even between closely related species like cattle and buffalo. In experimental studies, attempts to transfer Sarcocystis capracanis of goats to sheep (Pethkar and Shah 1982) failed. Likewise, large number of sporocysts of S. cruzi of cattle origin fed to naïve buffalo calves (Jain and Shah 1985a) and similar attempt on S. fusiformis and S. levinei of buffalo origin fed to cow calves (Ghoshal et al. 1987a) were unsuccessful. Prevalence in adult animals appeared much higher than young (<1 year) ones (Devi et al. 1998; Swarnkar et al. 1999; Srinivasa Rao and Hafeez 2002a). Highest prevalence was observed during monsoon/post- monsoon or even winter months depending on location (Jain and Shah 1985b; Mohanty et al. 1995a; Devi et al. 1998; Swarnkar et al. 1999), which is apparently related to the availability of moisture favouring the development and survival of the faecal sporocysts voided by the definitive hosts. The effect of a range of temperatures on the viability of S. levinei from cardiac muscle of buffalo (Srivastava et al. 1986) and S. miescheriana in pork (Saleque et al. 1990) indicated that infectivity was not affected except by extremely high or extremely low temperatures, which for S. capracanis was found to be 100 °C and −20 °C, respectively (Singh and Shah 1990a).
Cattle
The prevalence reported from various states was highly variable viz. 75.3 % of 1,030 animals in Bihar (Sahai et al. 1982), 80.3 % in Madhya Pradesh (Jain and Shah 1985b), 80.3 % of 86 in Orissa (Mohanty et al. 1995a) and 58.74 % of 143 from Tirupati in Andhra Pradesh (Venu and Hafeez 2000). These workers surveyed cattle as part of the overall prevalence of Sarcocystis in ruminants and as such, either did not identify the species or considered it solely as the dog-transmitted S. cruzi (Venu and Hafeez 2000).
Buffalo
According to Gill et al. (1978), Sarcocystis is the most common protozoan infection in the buffalo (Bubalus bubalis) and in general, the prevalence rate is higher than in cattle (Ramanujachari and Alwar 1951). Most subsequent workers confirmed this trend in their observations: 86.6 % of 60 examined in Orissa (Mohanty et al. 1995a), 79.9 % of 169 examined in A.P. (Venu and Hafeez 2000), with Sahai et al. (1982) who found 59 % positive of 283 buffaloes in Bihar, being an exception. In M.P., natural occurrence of Sarcocystis was recorded in 80 % buffalo carcasses (Ghoshal et al. 1986) with S. levinei as the predominant species in Marathwada (Deshpande et al. 1983a) as also in Bihar (Saha et al. 1986).
Goat
Chhabra and Mahajan (1978) observed Sarcocystis in the oesophagus and diaphragm tissues of slaughter goats by trypsin digestion in the course of attempted isolation of Toxoplasma at Chandigarh. At Hisar, Haryana, Gupta and Gautam (1982) found 55 % positivity by pepsin digestion of muscles from 152 goats. In M.P. Pethkar and Shah (1982) recorded 66.46 % positive in 650 goat carcasses, of which the dog-transmitted S. capracanis was predominant (97.20 %). A later report from Hisar (Juyal et al. 1989a) found microcysts identical to S. capracanis in 62.6 % of 235 slaughter goats. Other reports documented an alarming 100 % S. capracanis in 35 goats in Marathwada (Shastri 1990), 67.10 % of 76 at Jabalpur, M.P. (Singh et al. 1990), 33.33 % of 80 goats in Tripura (Saha and Ghosh 1992) 68.3 % of 703 from Bikaner, Rajasthan (Singh et al. 1991), 73.33 % in Uttar Pradesh (Daya Shankar and Bhatia 1993), 56.18 % in Maharastra (Wadajkar et al. 1994), 76.6 % of 120 goats in Orissa (Mohanty et al. 1995a), 55.26 % (Aulakh et al. 1997) from Punjab, 34.62 % of 800 goats at Nagpur (Jumde et al. 2000), and 72 % of 111 goats in A.P. (Venu and Hafeez 2000).
Pig
Sarcocystis infection in the musculature of slaughter pigs was reported from various centres. The prevalence of the dog-transmitted microcysts (likely S. miescheriana) was 53.5 % of 170 pigs in Bihar (Sahai et al. 1982), 68.8 % at Hisar (Gupta and Gautam 1984), 68.98 % in slaughter pigs in western U.P. (Agnihotri et al. 1987; Saleque and Bhatia 1991) 84 % in M.P. (Solanki et al. 1991a), 76.1 % of 372 in Guwahati, Assam (Devi et al. 1998), 61.48 % of 270 in A.P. (Srinivasa Rao and Hafeez 2002a), 73.36 % of 229 pigs at Ludhiana (Avapal et al. 2003a, 2004), Prasanth (1995) reported prevalence of two species viz. S. suihominis (48.9 %) and S. miescheriana (40.2 %) from Western U.P. Occasionally spontaneous Sarcocystis were also detected in the brain of pigs (Gupta and Iyer 1984; Prakash et al. 2007).
Sheep
Prevalence of Sarcocystis infection in sheep has been reported from various places viz. up to 81.15 % in Haryana (Gupta et al. 1979; Hussain et al. 1986) and elsewhere ranging between 19.73 and 73.40 % in A.P. (Rao and Rama Rao 1987), 71.87 % in U. P. (Saleque et al. 1992), 81.37 % in Orissa (Mohanty et al. 1995a), 74.3 % in Western U.P. and Delhi (Banerjee 1998), 37.3 % in Rajasthan (Swarnkar et al. 1999) and 88.32 % in Tirupati, A.P. (Venu and Hafeez 2000). Majority of these investigators regarded S. tenella as the only or the predominant species identified, whereas Saleque et al. (1992) assigned them to two species viz. S. tenella and S. arieticanis, both dog- transmitted, on morphological grounds.
Horse
The first report in India on the occurrence of Sarcocystis as sparsely distributed white bodies in the oesophageal and heart muscles of a pony, came from Madras (Achuthan and Raja 1990), but the species was not identified. The first confirmed case of S. equicanis was recorded in Punjab (Juyal et al. 1991). Chronic sarcocystosis in a naturally infected mare was reported later (Juyal et al. 1994).
Man
Sporadic human infection has been recorded incidentally during autopsy (Vasudevan 1927; Naidu 1928). The first documented case of Sarcocystis cysts diagnosed by histological examination of biopsy pieces from calf and deltoid muscles during life, came from Lucknow (Gupta et al. 1973). Ten years later, again from Lucknow, two more cases from living humans, were reported (Agarwal and Srivastava 1983). Both were presented with discharging sinuses, one on the lower extremity and the other, gluteal, but the presence of cysts was unrelated to the symptoms. The source of infection was not clear but appeared to be faecal or soil contamination of food. In these two instances, the species is likely to be S. lindemanni for which man is the intermediate host (Dubey et al. 1989a) and definitive host is not known.
Biology and speciation
Generally, microsarcocysts (<1 mm) belong to such species of Sarcocystis which have the definitive hosts as canids or humans (and possibly other primates), while the species having macrosarcocysts (>1 mm) involve felids as definitive hosts (Bhatia et al. 2010). Early investigators either did not identify beyond the genus level or relied on morphological criteria of cysts to differentiate between the species.
Cattle
Deshpande et al. (1983a) regarded the thin walled cysts as S. cruzi and the thick walled as S. hirsuta/S. suihominis. Of these three, S. cruzi is dog-transmitted and has been found to be the predominant species, 66.81 % out of the overall 83.2 % positivity in M.P. (Jain and Shah 1985b, 1987). The other two species, S. hirsuta and S. hominis, both have thick radially striated walls but have different definitive hosts viz. cat and man, respectively. The comparative morphology of Sarcocystis of the three species has been described (Pandit et al. 1994). In experimental infections of S. cruzi, the pattern of discharge of sporocysts in dog faeces was reported (Jain and Shah 1986b). Heart muscles from cattle fed to pups and kittens (Sahai et al. 1983) resulted in shedding of sporocysts by pups only in 12.0–15.3 days (prepatent period) but the authors did not name the species. For S. cruzi, Jain and Shah (1986b) recorded the prepatent period of 9–10 days post infection (DPI) and patent period as 18–85 DPI, while Venu and Hafeez (1999) found the prepatent period as 9–11 DPI.
Buffalo
The gametogenous development of bubaline S. fusiformis in pups in an experimental study (Chauhan et al. 1977) was reported. The first sporocyst appeared in faeces after 18–21 days and shedding continued for >140 days. On histological examination, the parasites were located mainly in the sub-epithelial tissues of the villi of the jejunum and upper ileum. On the other hand, Gill et al. (1978) fed diaphragm muscles from buffalo naturally infected with Sarcocystis but observed shedding of only unsporulated Isospora oocysts and no sporocysts. Transmission studies carried out at Madras (Achuthan 1983) indicated that cat was the definitive host for at least one species in buffaloes. Sarcocystis from buffalo was fed orally to three cats which started shedding of sporocysts in the faeces 18–21 days onwards. A buffalo calf fed with these sporocysts became ill 38 DPI and died 77 DPI. The parasite was identified as S. fusiformis. Feeding of naturally infected bubaline tissues/sarcocysts of S. levinei was found infectious to pups (Deshpande et al. 1982; Jain et al. 1985) which shed sporocysts in faeces. In another study, gametogonous development of S. levinei in the small intestine of dogs (Jain and Shah 1986a) was described. Buffalo-dog-buffalo cycle was further established by ensuing studies at Jabalpur (Ghoshal et al. 1987b, 1988). S. levinei was by far the commonest bubaline Sarcocystis species with predilection for oesophageal tissues and the patent period for sporocysts output in dogs was 15–74 days after being fed infected muscles (Ghoshal et al. 1988). Prevalence and intensity of S. fusiformis in Tarai region of U.P. was reported (Juyal and Bhatia 1989a). According to Dubey et al. (1989b), S. fusiformis forms large (up to 32 mm) sarcocysts in the oesophagus of buffaloes. In their opinion, the ultrastructure of sarcocyst wall is a useful criterion for distinguishing the species. As per Venu and Hafeez (1999), the prepatent period of S. levinei is 11–14 days. Large numbers of sporocyst output translates to enormous environmental contamination with this species in nature.
Goat
When Pethkar and Shah (1982) had reported the occurrence of two species in goats, the predominant microcysts were identified as S. capracanis, but the marginally present species which failed to develop in dogs and cats, was left unnamed. The assumption that man could be the definitive host of the species was tested experimentally (Chaudhary and Shah 1988) with the senior author volunteering to act as “guinea pig”, and swallowing, on two separate occasions, sarcocysts, but no sporocysts were recovered in his stools in 20 consecutive DPI. Thereby, it was concluded that the particular species did not utilize man as its definitive host. The prepatent period of S. capracanis in experimentally infected pups was found 9–15 (mean 12.25) days (Juyal et al. 1989a). Similar observations were recorded by Singh and Shah (1990b) on feeding offals from slaughter goats to dogs with 9 and 24 DPI as the prepatent and patent periods, respectively. Later, these authors (Singh et al. 1990) found high concentration of S. capracanis cysts in oesphagus and tail muscles of naturally infected goats, the latter site being suitable for detection in live animals. Diaphragm muscle was also commonly parasitized (Shastri 1990; Daya Shankar and Bhatia 1993; Wadajkar et al. 1994) and cardiac muscle next in frequency (Juyal et al. 1989a; Saha and Ghosh 1992). Evidence of occurrence of S. hircicanis in India was first provided by Srivastava et al. (1990) by morphological differentiation alone. More conclusive evidence came forth from Jabalpur (Sharma and Shah 1992) who not only reported S. hircicanis cysts having thin wall with hairy processes as against thick, radially striated wall of S. capracanis, but also on longer prepatent period (12–15 days as against 9 of S. capracanis) and larger size of sporocysts of S. hircicanis. Elsewhere, Daya Shankar and Bhatia (1993) from U. P. and Wadajkar et al. (1994) from Maharashtra, identified two species on the basis of cyst wall morphology viz. S. capracanis and S. hircicanis. Venu and Hafeez (1999) recorded prepatent period of S. capracanis as 9–10 days. Dafedar et al. (2008) recorded prevalence of S. capracanis and S. hircicanis as 72.24 and 21.12 % respectively at an abattoir in Bangalore, Karnataka.
Pig
Pig has been shown to harbour three species of Sarcocystis utilising different definitive host species. Dog is the principal definitive host of S. miescheriana. Musculature from pigs fed to clean dogs resulted in excretion of sporocysts (Gupta and Gautam 1984) with a prepatent period of 12 days. These workers, however, did not name the species. It was left to a team at Jabalpur (Solanki et al. 1990) to work out the endogenous phase of the life cycle of S. miescheriana. In experimentally infected piglets, first generation schizonts were seen on 7th DPI in the endothelial cells of the capillaries of the liver only. Second generation schizonts were encountered on 12 DPI in the endothelial cells of arterioles and capillaries of heart, liver and kidney. Partially matured sarcocysts were seen on 90 DPI and fully mature on 120 DPI in muscles, maximum in the penis muscles and minimum in diaphragm; The maturity of sarcocysts was confirmed by feeding them to dogs which started shedding sporulated sporocysts in their faeces from 9 DPI. The gametogonous and sporogonous phase of the life cycle of S. miescheriana was elucidated in experimentally infected dogs (Solanki et al. 1991b); All these stages were located inside parasitophorous vacuoles in lamina propria of jejunum and ileum of small intestine. The prepatent and patent periods in dogs were 9–10 and 39–40 days, respectively. Comparative morphology of sarcocysts of S. miescheriana and S. suihominis collected from naturally infected pigs was described, along with their metrocytes and cytozoites (Solanki et al. 1991c). From slaughter pigs at abattoirs in U. P., incidence of both the porcine species was identified (Saleque and Bhatia 1991). Prasanth and Bhatia (1996) reported the species of Sarcocystis of the pig in western U.P. and described the sporocyst of a till-then unrecognized species. Devi et al. (1998) reported Sarcocystis spp. in carcasses of pigs in Assam by feeding sarcocysts to cats and dogs and observation of the sporocysts shed. They identified S. miescheriana and S. suihominis. The comparative morphology of these two species was later described by Avapal et al. (2003b).
Sheep
Hussain et al. (1986) observed infection in sheep which they designated as S. ovicanis. Later, Saleque et al. (1992) recorded prevalence of two species in U.P. and identified them on morphological grounds as S. tenella and S. arieticanis. Swarnkar et al. (1999) found only S. tenella in sheep at an organized farm in Rajasthan. The most commonly involved muscles were diaphragm and oesophagus (Gupta and Gautam 1982; Saleque et al. 1992). Sporocyst output in dogs fed with sarcocysts of S. arieticanis and S. tenella was observed by Shrivastava and Jain (2001). In case of S. arieticanis, dogs started shedding sporocysts from day 10 to 12 PI, reached peak on 11 DPI and ceased from 21 DPI. Total sporocysts shed were estimated at 2.4 × 106. In S. tenella infected dogs, prepatent period was 10–12 days and patency took, another 8–12 days.
Horse
Juyal et al. (1991) recorded S. equicanis from the oesophageal and diaphragmatic muscles of a naturally infected mare and confirmed the identity by feeding the muscles to dog which shed sporocysts from 7 DPI till it was killed on 19 DPI.
Clinico-pathology
In contrast to their high rate of prevalence, Sarcocystis spp. were initially considered to be of doubtful pathogenicity. This view has since changed and studies have shown it to produce clinical disease with morbidity and mortality in livestock (Achuthan 1983). This author found that experimentally infected buffalo calf became ill 38 DPI and died 77 DPI with impression smears/sections from heart, oesophagus and tongue showing developing forms of Sarcocystis. Biochemistry of S. fusiformis of buffalo, covering total lipids, phospholipids, cholesterol, fatty acids and glycerides, total proteins, acetylcholinesterase, glutamate oxalo-acetate transaminase and glutamate-pyruvate transaminase in sarcocyst was reported (Chaudhary et al. 1985). In a subsequent paper (Chaudhary et al. 1986), the glycogen content and activities of alkaline and acid phosphatases of sarcocysts of S. fusiformis from naturally infected buffalo were determined biochemically and histochemically. They also observed that sarcocysts are well demarcated from the host tissue by a thick wall. The presence of alkaline phosphatase activity on this wall suggested that it was metabolically active. Hussain et al. (1986) described pathological changes in 5–9 weeks old lambs experimentally infected with the highly pathogenic S. ovicanis, 0.5–1.0 million sporocysts orally. All the lambs died 25–31 DPI with widespread and severe hemorrhages in all internal organs. Before dying, the lambs became anorectic and weak. On the other hand, the only pathology noted by Rao and Rama Rao (1987) in infected cattle and buffalo, was inflammatory response appearing as mild degenerative changes of the cell cytoplasm at the cyst lodgement site. Clearly, the pathogenic effects are dose-related, as observed by Juyal et al. (1989b) in experimentally induced S. capracanis infection in pregnant goats. A dose of 5,000 sporocysts produced no clinical signs, 10,000 produced clinical disease characterized by two peaks of fever 12 and 20 DPI, pale mucous membrane and premature delivery in one of the three goats, 25000 sporocysts caused more pronounced clinical effects as well as stillbirth in two of three goats. In the goats given 50,000 sporocysts, severe disease with premature expulsion of dead foetus or weaklings that died within 24 h, resulted. Cyst extracts of S. fusiformis of buffalo were toxic to inoculated laboratory rabbits and mice (Saleque et al. 1991). In experimentally infected pigs, S. miescheriana caused pathogenic effects mainly associated with 2nd generation schizonts (Solanki et al. 1992). Inflammatory response accompanied with gross and microscopic lesions were observed in different organs viz. heart, liver, lung, brain and spleen. A special feature was the inflammatory changes in the penile urethra of the male animals. Further clinico-haematologic and serum biochemical changes in S. miescheriana infected piglets were reported by Dey et al. (1995). The piglets suffered acute post-oral inoculation with 10/15 × 105 sporocysts and 4 of 12 died 16–19 DPI. The clinical symptoms observed were fever (up to 41.5 °C), anorexia, dullness, staggering gait, lacrimation and severe anaemia. Haematologically, decreased PCV and TEC, neutrophilia, lymphopenia and monocytosis were observed between 7 and 35 DPI. Total protein and albumin values were decreased whereas globulin values increased. The values of creatinine, blood urea nitrogen and bilirubin increased markedly. Wadajkar et al. (1995) observed clinical signs in kids orally inoculated with graded doses of S. capracanis which included two febrile peaks and death of all the six kids in 13–23 DPI. At necropsy, extensive petechial haemorrhages were observed on skeletal muscles, serosal surface of the alimentary canal, lymph nodes, urinary bladder and epicardium. Haemorrhagic lesions on trachea, aorta and auricles were consistent features in all the kids. Microscopic lesions consisted of severe congestion of blood vessels including capillaries, sinusoids and sub-endothelial haemorrhages. Pathology of Sarcocystis in natural infections in cattle, buffalo, sheep and goat, was reported by Mohanty et al. (1995b). In general, heavily infected animals were weak, debilitated and emaciated with pale mucous membranes, ascites, lymphadenopathy and in some cases, loss of hairs from switch of tail, particularly in cattle and buffalo. Histopathology in cattle revealed numerous cut cysts in cardiac and tongue muscles with loss of striation of some spots and hyalinization of infected muscle fibres considered confirmatory of S. cruzi. In buffaloes, the muscle around immature cysts showed loss of striation, vacuolation and fragmentation. The affected muscles were also infiltrated with aggregation of inflammatory cells predominantly lymphocytes. Intramuscular oedema was also present. The findings indicated the high pathogenicity of S. fusiformis. In sheep, infected tongue and oesophagus showed loss of striation, oedema and infiltration of inflammatory cells along with rupture of some muscle fibres contrary to the earlier report of Hussain et al. (1986), severe haemorrhages in internal organs, were not observed in any of the sheep. In a later study on pathogenesis of S. tenella, Banerjee (1998) observed clinical signs of fever, severe anaemia and neurological symptoms in infected sheep. Haemorrhaegic lesions were seen in the sites of development of schizonts viz. endothelial cells of internal organs, including liver, kidney, CNS, cardiac and skeletal muscles, spleen, mesenteric lymph nodes and lungs. These changes were coupled with significant rise in the total protein concentration and cell counts in CSF were constant features in acutely infected lambs. The LD50 was 25,000 sporocysts. Pathological changes in natural Sarcocystis infected pigs were essentially similar viz. hidebound condition, emaciation and haemorrhages on the serosal surface of the large intestines and viscera including lungs (Avapal et al. 2004). Heart surface also showed haemorrhages, congestion and atrophy of fat which imparted a grey gelatinous appearance in a number of cases. In a slaughter house based study on cattle (Patra et al. 2006), infected muscles showed inflammatory reaction with necrosis, congestion, myositis and disorganization of muscle fibres. Histopathologically, villar projections of the cyst wall were observed. The chambers were uniform in size with a clear cord like structure. Sporozoites were aggregated at the junction of the central and peripheral area. Although not recorded so far in India, the discovery of S. neurona as the primary cause of equine protozoal myeloencephalitis (EPM) is a major recent development that has captured the imagination of researchers abroad (Dubey 2001).
Diagnosis
Since no stage of these intracellular protozoa comes out of the infected/diseased intermediate host, diagnosis in the living animal is not easy. Clinical signs of Sarcocystis are vague and do not allow more than a presumptive diagnosis. In the acute phase, these are anaemia, fever, anorexia, excessive salivation and abortion. In chronic phase, growth is retarded and there is loss of hair especially from the switch of the tail, neck and rump (Dubey et al. 1989b). Therefore, biopsy from tail muscle has been suggested as a means to monitor the development of sarcocysts in S. capracanis in live animals (Singh et al. 1990). At postmortem, various techniques viz. peptic digestion for 2 h, histological examination and teasing out of cysts from musculature in NSS, have been variously assessed and compared (Juyal et al. 1988, 1989c; Avapal et al. 2003a). Like in many other parasites, immunodiagnosis has also been tried. Evidence of cell-mediated immunity was found in kids experimentally inoculated with S. capracanis sporocysts (Juyal et al. 1989d). Delayed type of hypersensitivity as well as antigen induced inhibition of migration of leucocytes was noticed on 28 DPI. Double immunodiffusion and single radial immunodiffusion for detection of antibody responses against cystic antigen of S. fusiformis (Juyal et al. 1990), preparation and assessment of partially purified antigens of S. cruzi and S. hirsuta for immunodiffusion, immuno electrophoresis (IE) and precipitation tests to detect serum antibodies (Pandit et al. 1993), and attempts to detect seroprevalance in swine (Avapal et al. 2002) have not been satisfactory due to cross-reactivity, not only with other Sarcocystis spp. but even related genera like Toxoplasma and Hammondia. In serodiagnosis, high sensitivity of dot–ELISA test as compared to double immunodiffusion and counter immunoelectrophoresis (CIE) was reported in serum sample of cattle in Punjab (Singh et al. 2004). Serodiagnosis in buffaloes was performed (Rohini and Hafeez 2005) with three tests viz. gel diffusion, IE and CIE, of which CIE gave the highest (90 %) sensitivity. Persistence of serum antibodies to S. neurona could be detected in horses many years after their relocation from the endemic North America to India (Brown et al. 2006). Recently a molecular technique PCR has been used for species specific identification of Taenia spp. cysticerci and Sarcocystis cysts from infected pigs and cattle (Gonzalez et al. 2006) opening up potential new approach for species specific diagnosis of sarcocystosis.
Control and treatment
The key to control is in the interruption of the life cycle (Bhatia et al. 2010) by preventing the carnivore definitive hosts from: (a) eating raw meat of offals of slaughtered/dead animals, and (b) contaminating the feed and water of livestock with their faeces. For chemotherapy, anticoccidials are recommended in a general way and have been tried in a few instances. Sulphadiazine, Bifuran (a combination of nitrofurazone and furazolidone) at 10 mg/kg and pyremethamin at 1 mg/kg b. wt. were ineffective in treating pups experimentally infected with S. levinei (Deshpande et al. 1983b). Salinomycin at 5 mg/kg b. wt. was found effective in experimental sarcocystosis (S. capracanis) in goats (Kumar et al. 1988) as well as at 4 mg/kg b. wt. in dogs. On the other hand, dogs treated with sulphamethoxazole and trimethoprim showed no response. Singh et al. (1990) used 20 % amprolium in experimentally infected pups and observed reduction in sporocysts per gram (SPG) counts in the faeces. Oral amprolium at 100 mg/kg b. wt. and Maduramycin at 150 mg/kg b. wt. given to pups experimentally infected with Sarcocystis infected pork, for 5 days starting from the day when the faecal sporocysts were first sighted, resulted in 93.93 and 96.96 % reduction in SPG, in the respective treatment groups (Srinivasa Rao and Hafeez 2002b).
Zoonotic aspects
Only two species, S. hominis and S. suihominis infect humans as definitive hosts with cattle and pigs, respectively as the intermediate hosts (Dubey et al. 1989b). However, man is unique in being the definitive host for these species as well as intermediate host for some other. The relatively low prevalence of S. hominis in India can be attributable to human factors viz. non-consumption of beef on religious grounds by majority Hindu population and the habit of thorough cooking among the minority beef-eaters (Shah 1990). On the other hand, the prevalence of S. suihominis is high (Solanki et al. 1991c; Saleque and Bhatia 1991; Prasanth 1995), which is not unexpected given the backyard rearing of pigs with opportunity for scavenging of human faeces in the slums as well as unhygienic slaughter, handling and consumption of pork by slum dwellers (Shah 1995). Evidence of S. suihominis infection in humans was provided by a report from Pantnagar (Banerjee et al. 1994). These workers found positivity in stool samples of 14 out of 20 children belonging to families who slaughter pigs without any meat inspection procedure. Further, these children had the history of consuming raw pork and passed typical sporocysts in stools, with frequent diarrhoea and abdominal pain, which may not be exclusively due to intestinal sarcocystosis. However, such reports are isolated and human intestinal sarcocystosis appears under-reported in India. Cases of muscular or extra intestinal sarcocystosis among humans, as recorded, have been mentioned in the aforesaid but their clinical significance is unknown. Sarcocystosis has been regarded in India as an emerging zoonosis (Juyal and Bhatia 1989b) but has remained primarily of academic interest only.
Conclusion
Sarcocystosis has been regarded as an emerging disease largely because most of the information on this group of parasites, particularly the life cycles and association with disease, is recent. Much of what we know of sarcocystosis in India relates to prevalence which is high by practically all accounts. The species identification based on cyst morphology and without transmission studies, remains empirical. The problem is further compounded by the knowledge that more than one species of Sarcocystis in an intermediate host can cycle through a given definitive host. For example, in sheep there are four species, two each transmissible via dogs and cats. As of present status, dog is the final host of at least one species each prevalent in cattle, buffalo, goat, pig and sheep in India and for some other, cat is the definitive host. This merits to be considered against the background that there are at least 21 species of Sarcocystis that are found in dog faeces, and at least ten in cat faeces (Dubey 2009). There are issues which need to be addressed by future research. It would be of interest to look for the species considered non-existent in India so far. In this context, looking out for S. neurona, the pathogenic species in the horse should be a priority. Sarcocystis of the dromedary camel (Camelus dromedarius) reported from other camel-rearing countries (Latif and Khamas 2007), has not been explored in India despite a sizeable camel population. There is variation in prevalence rate in the striated and cardiac musculature and to some extent, the CNS. Is this only due to predilection or more can be interpreted if such findings are consistent? Also, experimental work has indicated that only high dose of infection leads to clinical symptoms and/or mortality. How far that sort of situation prevails in nature, extent of soil/pasture contamination by sporocysts, morbidity and economic impact need to be worked out. As clinical diagnosis is difficult, application of molecular biology to devise specific diagnostic techniques is another fertile area for future development. In view of the zoonotic importance of related coccidians like Toxoplasma and Cryptosporidium, medical and public health authorities should be taken aboard to collaborate in the surveillance for the zoonotic potential of S. suihominis.
References
Achuthan HN (1983) Sarcocystis and sarcocystosis in buffalo (Bubalus bubalis) calves. Indian Vet J 60:344–346
Achuthan HN, Raja EE (1990) Occurrence of Sarcocystis spp. in horse (Equus caballus). Indian Vet J 67:472
Agarwal PK, Srivastava AN (1983) Sarcocystosis in man: a report of two cases. Histopathology 7:783–787
Agnihotri RK, Juyal PD, Bhatia BB (1987) Microsarcocysts in pigs in Uttar Pradesh, India. J Vet Parasitol 1:69–70
Aulakh RS, Joshi DV, Juyal PD (1997) Prevalence of Sarcocystis capracanis in naturally infected goats: a preliminary report. J Vet Parasitol 11:99–100
Avapal RS, Singh BB, Sharma JK, Juyal PD (2002) Sero-prevalence of sarcocystosis in swine. J Parasitic Dis 26:109–110
Avapal RS, Sharma JK, Juyal PD (2003a) Comparison of techniques to detect Sarcocystis infection in slaughtered pigs. J Vet Parasitol 17:65–66
Avapal RS, Sharma JK, Juyal PD (2003b) Comparative morphology of Sarcocystis suihominis and S. miescheriana in domestic pigs. Indian J Anim Sci 75:392–393
Avapal RS, Sharma JK, Juyal PD (2004) Pathological changes in Sarcocystis infection in domestic pigs (Sus scrofa). Vet J 168:358–361
Banerjee PS (1998) Studies on pathogenesis of Sarcocystis tenella in sheep. J Vet Parasitol 12:65
Banerjee PS, Bhatia BB, Pandit BA (1994) Sarcocystis suihominis infection in human beings in India. J Vet Parasitol 8:57–58
Bhatia BB, Pathak KML, Juyal PD (2010) Text book of veterinary parasitology, 3rd edn. Kalyani Publishers Ludhiana, New Delhi, pp 497–506
Brown CM, Morrow JK, Carleton CL, Ramanathan B, Reddy R, Vaidya V, Kathikeyan SM, Zulfkar AA, Kannadkar VS (2006) Persistence of serum antibodies to Sarcocystis neurona in horses moved from North America to India. J Vet Intern Med 20:994–997
Chaudhary RK, Shah HL (1988) Role of man in the life cycle of Sarcocystis spp. of the goat (Capra hircis). Indian Vet J 65:742
Chaudhary PK, Kushwah HS, Shah HL (1985) Biochemistry of the sarcocyst of Sarcocystis fusiformis of buffalo Bubalus bubalis. Vet Parasitol 17:295–298
Chaudhary RK, Kushwah HS, Shah HL (1986) Biochemical and histochemical studies of the sarcocyst of Sarcocystis fusiformis of buffalo Bubalus bubalis. Vet Parasitol 21:271–273
Chauhan PPS, Bhatia BB, Agrawal RD, Katara RK, Ahluwalia SS (1977) On the gametogenic development of bubaline Sarcocystis fusiformis in pups—an experimental study. Indian J Exp Biol 15:492–494
Chhabra MB, Mahajan RC (1978) Sarcocystis spp. from the goat in India. Vet Record 103:562–563
Chhabra MB, Mahajan SK, Gupta SL, Gautam OP (1984) Sporozoa and helminths encountered in faeces of cats from North India. Indian J Parasitol 8:211–213
Dafedar AM, D’Souza PE, Anand KJ, Puttalakshmamma GC (2008) Prevalence of sarcocystosis in goats slaughtered in an abattoir in Bangalore, Karnataka state. Vet World 1:335–337
Deshpande AV, Shastri UV, Deshpande MS (1982) Experimental infection in pups by feeding cysts of Sarcocystis levinei Dissanaike and Kan (1978). Indian J Parasitol 6:331–332
Deshpande AV, Shastri UV, Deshpande MS (1983a) Prevalence of Sarcocystis in cattle and buffaloes in Marathwada, India. Trop Vet Anim Sci Res 1:92
Deshpande AV, Shastri UV, Deshpande MS (1983b) Ineffective treatment of Sarcocystis levinei in dogs. Trop Vet Anim Sci Res 1:92
Devi PP, Sarmah PC, Saleque A (1998) Sarcocystis spp. infection in carcasses of domestic pigs in Guwahati, Assam. J Vet Parasitol 12:56–57
Dey S, Khatkar SK, Ghosh JD, Akbar MA (1995) Clinico-haematological and serum biochemical changes in piglets experimentally infected with Sarcocystis meischeriana. Indian Vet J 72:126–130
Dubey JP (2001) Recent developments in the biology of Sarcocystis ncurona and equine protozoal myeloencephalitis (EPM). J Vet Parasitol 15:91–102
Dubey JP (2009) The evolution of knowledge of cat and dog coccidia. Parasitology (Camb.) 136:1469–1475
Dubey JP, Speer CA, Fayer R (1989a) Sarcocystosis of animals and man. CRC Press, Boca Raton
Dubey JP, Speer CA, Shah HL (1989b) Ultrastructure of sarcocysts from water buffalo in India. Vet Parasitol 31:149–152
Ghoshal SB, Joshi SC, Shah HL (1986) A note on the natural occurrence of Sarcocystis in buffaloes (Bubalus bubalis) in Jabalpur region, M.P. Indian Vet J 63:165–166
Ghoshal SB, Joshi SC, Shah HL (1987a) Cross-transmission studies of Sarcocystis fusiformis and S. levinei of buffalo to cattle calves. Indian J Anim Sci 57:524–525
Ghoshal SB, Joshi SC, Shah HL (1987b) Development of sarcocysts of Sarcocystis levinei in water buffalo infected with sporocysts from dogs. Vet Parasitol 26:165–167
Ghoshal SB, Joshi SC, Shah HL (1988) Sporocyst output in dogs fed sporocysts of Sarcocystis levinei of the buffalo (Bubalus bubalis). Vet Parasitol 28:173–174
Gill HS, Singh A, Vadehra DV, Sethi SK (1978) Shedding of unsporulated Isospora oocysts in faeces by dogs fed diaphragm muscles from water buffalo (Bubalus bubalis) naturally infected with Sarcocystis. J Parasitol 64:549–551
Gonzalez LM, Villalobos N, Montero E, Marales J, Alamo Sanz R, Muro A, Harrison LJS, Parkhouse RME, Garate T (2006) Differential molecular identification of Taeniid spp. and Sarcocystis spp. cysts isolated from infected pigs and cattle. Vet Parasitol 142:95–101
Gupta SL, Gautam OP (1982) Sarcocystis infection in goats in Hisar and its transmission to dogs. Indian J Parasitol 6:73–74
Gupta SL, Gautam OP (1984) Sarcocystis infection in pigs of Hisar and its transmissin to dogs. Vet Parasitol 16:1–3
Gupta SC, Iyer PKR (1984) Spontaneous sarcocystosis in brain of pigs. Indian J Anim Sci 61:938–939
Gupta OK, Nath P, Bhatia KB, Mehrotra RML (1973) Sarcocystis infection in man, a case report. Indian J Path Bacteriol 16:73–75
Gupta SL, Gautam OP, Bhardwaj RM (1979) A note on the prevalence of Sarcocystis infection in sheep from Hisar area as studied by pepsin digestion technique. Indian J Anim Sci 49:971–972
Hussain MM, Gupta SL, Singh RP, Verma PC (1986) Pathological changes in lambs infected with Sarcocystis ovicanis during experiments. Indian J Anim Sci 56:169–173
Jain PC, Shah HL (1985a) Cross-transmission studies of Sarcocystis cruzi of the cattle to the buffalo calves. Indian J Anim Sci 55:27–28
Jain PC, Shah HL (1985b) Prevalence and seasonal variations of Sarcocystis of cattle in Madhya Pradesh. Indian J Anim Sci 55:29–31
Jain PC, Shah HL (1986a) Experimental study on gametogenic development of Sarcocystis levinei in the small intestine of dogs. Indian J Anim Sci 56:314–318
Jain PC, Shah HL (1986b) Determination of sporocysts discharged during the patent period by dogs fed with sarcocysts of Sarcocystis cruzi from cattle. Indian J Anim Sci 56:1005–1008
Jain PC, Shah HL (1987) Sarcocystis hominis in cattle in Madhya Pradesh and its public health importance. Indian Vet J 64:650–654
Jain AK, Gupta SL, Singh RP (1985) Discharge of sporocysts from pups fed with Sarcocystis levinei infected meat. Indian J Parasitol 9:77
Jumde PD, Bhojne GR, Maske DK, Kolte SW (2000) Prevalence of sarcocystosis in goats at Nagpur. Indian Vet J 77:662–663
Juyal PD (1991) Sarcocystis and sarcocystosis in India. South East Asian J Trop Med Pub Health 22:138–141
Juyal PD, Bhatia BB (1989a) Prevalence and intensity of Sarcocystis fusiformis infection in water buffaloes (Bubalus bubalis) in Tarai region of Uttar Pradesh. Indian Vet Med J 13:269–271
Juyal PD, Bhatia BB (1989b) Sarcocystosis: an emerging zoonosis. Indian Vet Med J 13:66–69
Juyal PD, Ruprah NS, Chhabra MB (1988) Assessment of efficacy of different techniques for microscopic Sarcocystis sp. in oesophageal muscle. Indian J Parasitol 12:185–186
Juyal PD, Ruprah NS, Chhabra MB (1989a) Incidence of microsarcocyst infection in slaughter goats (Capra hircus) at Hisar, Haryana. Indian J Parasitol 13:13–16
Juyal PD, Ruprah NS, Chhabra MB (1989b) Experimentally induced Sarcocystis capracanis infection in pregnant goats. Indian Vet med J 13:200–202
Juyal PD, Ruprah NS, Chhabra MB (1989c) Rapid isolation of intact micro-sarcocystis (Protozoa–Apicomplexa) cysts from muscular tissue. Indian J Anim Health 28:69–70
Juyal PD, Ruprah NS, Chhabra MB (1989d) Evidence for a cellular immune response in experimental caprine sarcocystosis. Indian J Parasitol 13:197–200
Juyal PD, Saleque A, Bhatia BB (1990) Double immunodiffusion (DID) and single radial immune diffusion (SRID) for detection of antibody responses against cystic antigen of Sarcocystis fusiformis. J Vet Parasitiol 4:83–85
Juyal PD, Kalra IS, Bali HS (1991) Occurrence of Sarcocystis equicanis in a horse (Equus caballus) in India. J Vet Parasitol 5:53–54
Juyal PD, Gupta MP, Gupta PP, Singh H, Kalra IS (1994) Chronic Sarcocystis infection in a naturally infected mare. Indian Vet Med J 18:54–55
Kumar A, Srivastava PS, Sinha SRP (1988) Chemotherapy of experimental caprine sarcocystosis in goats and young dogs with salinomycin (Coxistac Pfizer). J Vet Parasitol 2:129–132
Latif BMA, Khamas WA (2007) Light and ultrastructure morphology of sarcocystosis in one-humped camel (Camelus dromedarius) in northern Jordan. J Camel Pract Res 14:45–48
Mamatha GS, D’Souza PE, Bhat MN (2006) Gastrointestinal parasitism in dogs and cats in Bangalore. Intas Polivet 6:152–153
Mohanty BN, Misra SC, Panda DN, Panda MR (1995a) Prevalence of Sarcocystis infection in ruminants in Orissa. Indian Vet J 72:1026–1030
Mohanty BN, Misra SC, Rao AT (1995b) Pathology of Sarcocystis infection in naturally infected cattle, buffalo, sheep and goats. Indian Vet J 72:569–571
Naidu AS (1928) A case of sarcosporidiosis. Lancet 1:549–550
Pandit BA, Garg SK, Bhatia BB (1993) Preparation and assessment of precipitating antigens of Sarcocystis cruzi and S. hirsuta. J Vet Parasitol 7:5–15
Pandit BA, Bhatia BB, Banerjee PS (1994) Comparative morphology of sarcocysts of three species of Sarcocystis in cattle from Tarai region of Uttar Pradesh. J Vet Parasitol 8:53–59
Patra NC, Bera AK, Bhattachary D, Karmarkar PK (2006) Pathomorphology and histochemistry of sarcocystosis in naturally infected bovines. Environ Ecol 24:98–102
Pethkar DK, Shah HL (1982) Prevalence of Sarcocystis in Madhya Pradesh. Indian Vet J 59:110–114
Prakash A, Sai Kumar G, Rout M, Nagarajan K, Kumar R (2007) Neurocysticercosis in free roaming pigs—a slaughter house survey. Trop Anim Health Prod 39:391–394
Prasanth Hema (1995) Sarcocystis infection in domestic pigs in Uttar Pradesh. J Vet Parasitol 9:119–123
Prasanth Hema, Bhatia BB (1996) On the species of Sarcocystis of pigs in Uttar Pradesh, India with a description of a sarcocyst of hitherto unrecognized species. J Vet Parasitol 10:57–61
Ramanujachari G, Alwar VS (1951) Indian Vet J 27:264 cited by Achuthan HN 1983
Rao G, Rama Rao P (1987) A note on Sarcocystis in domestic animals of Andhra Pradesh. Indian Vet J 64:614–615
Rohini K, Hafeez Md (2005) Serodiagnosis of sarcocystosis in buffaloes. Indian Vet J 82:330–331
Saha AK, Ghosh D (1992) Prevalence of sarcocysts in black Bengal goats in Tripura. Indian Vet J 69:82–83
Saha AK, Srivastava PS, Sahai BN, Sinha SRP, Singh SP (1986) Prevalence of sarcocystosis in slaughtered cattle and buffaloes in Bihar. Indian J Parasitol 10:63–66
Sahai BN, Singh SP, Sahay MN, Srivastava PS, Juyal PD (1982) Note on the incidence and epidemiology of Sarcocystis infection in cattle, buffaloes and pigs in Bihar. Indian J Anim Sci 52:1005–1006
Sahai BN, Singh SP, Sahay MN, Srivastava PS, Juyal PD (1983) Role of dogs and cats in the epidemiology of bovine sarcosporidiosis. Indian J Anim Sci 53:84–85
Saleque A, Bhatia BB (1991) Prevalence of Sarcocystis in domestic pigs in India. Vet Parasitol 40:151–153
Saleque A, Bhatia BB, Juyal PD (1990) Sarcocystis infection in goats (Capra hircus) in Tarai region of Uttar Pradesh. Indian Vet Med J 14:276–277
Saleque A, Bhatia BB, Juyal PD, Rehman H (1991) Toxic effects of cyst extracts of Sarcocystis fusiformis of buffaloes in rabbits and mice. Vet Prasitol 38:61–65
Saleque A, Bhatia BB, Shankar D (1992) Prevalence of two species of Sarcocystis in sheep in Uttar Pradesh. India Vet J 69:841–842
Shah HL (1990) Human factors in dissemination and transmission of Sarcocystis infection. Lead Paper-1, Proc. First Asian Cong Vet Parasitol 26–28 November. Bihar Veterinary College, Patna, pp 1–8
Shah HL (1995) Sarcocystis as a zoonosis with special reference to India—review article. J Vet Parasitaol 9:57–61
Shankar Daya, Bhatia BB (1993) Sarcocystis infection in goats in Uttar Pradesh. Indian J Anim Sci 63:284–287
Sharma RK, Shah HL (1992) Occurrence of Sarcocystis hircicanis in the goat. Indian J Anim Sci 62:561–563
Shastri UV (1989) Prevalence of Sarcocystis and other coccidial infections in stray dogs in and around Parbhani town (Maharashtra). Indian Vet J 66:593–596
Shastri UV (1990) Sarcocystis infection in goats in Maharashtra. Indian Vet J 67:70–71
Shrivastava G, Jain PC (2001) Sporocyst output in dogs fed with sarcocysts of Sarcocystis arieticanis and S. tenella of sheep. Indian Vet J 78:533–535
Singh KP, Shah HL (1990a) Viability and infectivity of sarcocysts of Sarcocystis capracanis of the goat after maintaining them at different temperatures. Indian J Anim Sci 60:429–430
Singh KP, Shah HL (1990b) Offals from slaughtered goats as a source of infection of Sarcocystis capracanis for dogs. Indian J Anim Sci 60:1315
Singh KP, Agrawal MC, Shah HL (1987) Prevalence of Sarcocystis sporocysts in stray dogs. Indian J Anim Sci 57:1101–1102
Singh KP, Agrawal MC, Shah HL (1990) Prevalence of Sarcocystis capracanis in oesophagus and tail muscles of naturally infected goats. Vet Parasitol 36:153–155
Singh L, Raisinghani PM, Pathak KML, Kumar D, Manohar GS, Bhan AK, Arora JK, Swarnkar CP (1991) Epidemiology of Sarcocystis capracanis in goats at Bikaner, Rajasthan, India. Indian J Anim Sci 62:1044–1045
Singh BB, Sharma JK, Juyal PD, Gill JPS (2004) Seroprevalence of Sarcocystis species of cattle in Punjab. J Vet Parasitol 18:75–76
Solanki PK, Shrivastava HOP, Shah HL (1990) Endogenous life-cycle of Sarcocystis miescheriana in experimentally infected pigs. Indian J Anim Sci 60:1274–1278
Solanki PK, Shrivastava HOP, Shah HL (1991a) Prevalence of Sarcocystis in naturally infected pigs in Madhya Pradesh with an epidemiological explanation for the higher prevalence of Sarcocystis suihominis. Indian J Anim Sci 61:820–821
Solanki PK, Shrivastava HOP, Shah HL (1991b) Gametogonus and sporogonous life cycle of Sarcocystis miescheriana of the pigs in experimentally infected dogs. Indian J Anim Sci 61:374–378
Solanki PK, Shrivastava HOP, Shah HL (1991c) Morphology of sarcocysts of Sarcocystis miescheriana (Kuhn 1865) Labbe 1899 and Sarcocystis suihominis (Tadros and Laarman, 1976), Heydorn, 1977 from naturally infected domestic pig (Sus scrofa domestica) in India. Indian J Anim Sci 61:1030–1033
Solanki PK, Shrivastava HOP, Shah HL, Awadhiya RP (1992) Pathology of Sarcocystis miescheriana in experimentally infected pig. Indian J Anim Sci 62:797–801
Srinivasa Rao K, Hafeez Md (2002a) Incidence of porcine sarcocystosis in Andhra Pradesh. J Parasitic Dis 26:69–71
Srinivasa Rao K, Hafeez Md (2002b) Efficacy of amprolium and maduramycin against sarcocystosis in experimentally infected pups. J Parasitic Dis 26:111–113
Srivastava PS, Saha AK, Sinha SRP (1986) Effect of heating and freezing on the viability of sarcocysts of Sarcocystis levinie from cardiac tissue of buffaloes. Vet Parasitol 19:329–332
Srivastava PS, Kumar A, Sinha SRP, Sinha AK (1990) Morphological differentiation of caprine Sarcocystis species: evidence of occurrence of Sarcocystis hircicanis in India. First Asian Cong Vet Parasitol, Patna, 26–28 November. Abstract no. 51: 38, pp 55
Swarnkar CP, Khan FA, Juyal PD, Singh D, Bhagwan PSK (1999) Sarcocystis tenella in sheep in an organised farm of Rajasthan. Indian J Small Ruminants 5:28–30
Vasudevan A (1927) A case of sarcosporidial infection in man. Indian J Med Res 15:141
Venu R, Hafeez Md (1999) Prepatent period in dogs experimentally infected with Sarcocystis spp. Indian Vet J 76:574–576
Venu R, Hafeez Md (2000) Prevalence of Sarcocystis infections in slaughtered domestic ruminants in Tirupati (A.P.). Indian Vet J 77:165–166
Wadajkar SV, Shastri UV, Narladkar BW (1994) Prevalence of caprine Sarcocystis spp. in Marathwada region. J Vet Parasitol 8:43–46
Wadajkar SV, Shastri UV, Narladkar BW (1995) Caprine sarcocystosis: clinical signs, gross and microscopic pathology. Indian Vet J 72:224–228
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Chhabra, M.B., Samantaray, S. Sarcocystis and sarcocystosis in India: status and emerging perspectives. J Parasit Dis 37, 1–10 (2013). https://doi.org/10.1007/s12639-012-0135-y
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DOI: https://doi.org/10.1007/s12639-012-0135-y