Abstract
The present study was designed to investigate the anticancer activity of 4,7-dimethoxyflavanone in vitro. When human breast cancer MCF-7 cells were treated with 4′,7-dimethoxyflavanone at various concentrations (1–200 μM) for 24 h, antiproliferative effects were first observed at 1 μM and the IC50 was 115.62 μM. Conversely, 4′,7-dimethoxyflavanone was not cytotoxic (measured as lactate dehydrogenase release in CHO-K1 cells) under the same conditions. MCF-7 cells exposed to the 4′,7-dimethoxyflavanone at the IC50 concentration showed cell cycle arrest and apoptosis. Compared to the respective control level, exposure to 4′,7-dimethoxyflavanone resulted in a remarkable increase of small DNA fragments at the sub-G1 phase and an increase in the G2/M phase cell population. Moreover, when 4′,7-dimethoxyflavanone treatment caused G2/M phase arrest, an increase in CDK1 together with an increase in cyclin B was observed. Based on these results, 4′,7-dimethoxyflavanone may be a useful anticancer agent.
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Choi, E.J., Lee, J.I. & Kim, GH. Effects of 4′,7-dimethoxyflavanone on cell cycle arrest and apoptosis in human breast cancer MCF-7 cells. Arch. Pharm. Res. 34, 2125–2130 (2011). https://doi.org/10.1007/s12272-011-1216-7
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DOI: https://doi.org/10.1007/s12272-011-1216-7