Abstract
Dwarf bunt of wheat, caused by Tilletia controversa Kühn, is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 μg of teliospores in a 25-μL PCR reaction mixture.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- CAPS:
-
cleavage amplified polymorphic sequence
- ISSR:
-
inter-simple sequence repeat
- ITS:
-
internal transcribed spacer
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplification of polymorphic DNA
- RFLP:
-
restriction fragment length polymorphism
- SCAR:
-
sequence characterized amplified region
- SSR:
-
simple sequence repeat
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Gao, L., Chen, W.Q. & Liu, T.G. Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kühn . Folia Microbiol 55, 258–264 (2010). https://doi.org/10.1007/s12223-010-0038-1
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DOI: https://doi.org/10.1007/s12223-010-0038-1