Abstract
In this study, the kinetics of gravity-dependent movement of amyloplasts (statoliths) along root statocytes and hypocotyls (endodermis cells) has been analyzed and compared in order to testify cytoskeleton involvement in the displacement of statoliths in cress (Lepidium sativum L.) seedling statocytes. After 32 h of growth at 1 g or under a fast clinorotation (50 rpm), the seedlings were treated for 24 min as follows: exposition to clinorotation or 180° inversion and the action of gravitational force in root tip or hypocotyl tip direction. Statolith displacement was studied by light microscopy on semi-thin longitudinal sections of hypocotyls and root caps, measuring the distance between the centre of plastids and morphological cell bottom. Considerable temporal differences have been determined between the kinetics of the longitudinal motion of amyloplasts in root and hypocotyl statocytes of 1-g seedlings upon exposition to fast clinorotation and inversion. In statocytes of both organs of seedlings grown under fast clinorotation, the gravity provoked displacement of statoliths in the direction of its action; however, the displacement was much faster in hypocotyl than in root statocytes. It has been assumed that the gravity-determined longitudinal transport of amyloplasts, both in hypocotyl endodermic cells and root statocytes of cress seedlings, is modulated by the cytoskeleton.
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References
Baluška, F., Hasenstein, K.H.: Root cytoskeleton: its role in perception of response to gravity. Planta 203, 69–78 (1997)
Björkman, T.: Perception of gravity by plants. Adv. Bot. Res. 15, 1–41 (1988)
Driss-Ecole, D., Jeune, B., Prouteau, M., Julianus, P., Perbal, G.: Lentil root statoliths reach a stable state in microgravity. Planta 211, 396–405 (2000)
Driss-Ecole, D., Lefranc, A., Perbal, G.: A polarized cell: the root statocyte. Physiol. Plant. 118, 305–312 (2003)
Gaina, V., Švegždienė, D., Raklevičienė, D., Koryznienė, D., Stanevičienė, R., Laurinavičius, R.: Kinetics of amyloplasts movement in cress root statocytes under different gravitational loads. Adv. Space Res. 31, 2275–2281 (2003)
Grolig, F., Pierson, E.S.: Cytoplasmic streaming: from flow to track. In: Staiger, C.J., Baluška, F., Volkmann, D. (eds.) Actin: A Dynamic Framework for Multiple Cell Functions, pp. 557–571. Kluwer, Norwell (2000)
Hou, G., Mohamalawari, D.R., Blancaflor, E.B.: Enhanced gravitropism of roots with disrupted cap actin cytoskeleton. Plant Physiol. 131, 1360–1373 (2003)
Iversen, T.H., Pedersen, K., Larsen, P.: Movement of amyloplasts in the root cap cells of geotropically sensitive roots. Physiol. Plant. 21, 811–819 (1968)
Kato, T., Morita, M.T., Tasaka, M.: Role of endodermal cell vacuoles in shoot gravitropism. J. Plant Growth Regul. 21, 113–119 (2002)
Kiss, J.Z.: Mechanisms of early phases of plant gravitropism. Crit. Rev. Plant Sci. 19, 551–573 (2000)
Kumar, N.S., Stevens, M.H.H., Kiss, Z.J.: Plastid movement in statocytes of the ARG1 (altered response to gravity) mutant. Am. J. Bot. 95(2), 177–184 (2008)
Laurinavičius, R., Švegždienė, D., Gaina, V.: Force sensitivity of plant gravisensing. Adv. Space Res. 27(5), 899–906 (2001)
Laurinavičius, R., Švegždienė, D., Sievers, A., Buchen, B., Tairbekov, M.: Statics and kinetics of statolith positioning in cress root statocytes (Bion-11 Mission). Gravit. Space Biol. Bull. 11(1), 50 (1997)
Palmieri, M., Kiss, Z.J.: Disruption of the F-actin cytoskeleton limits statolith movement in Arabidopsis hypocotyls. J. Exp. Bot. 56(419), 2539–2550 (2005)
Palmieri, M.M., Schwind, M.A., Stevens, R.E., Edelmann, R.E., Kiss, Z.J.: Effects of the myosin ATPase inhibitor 2, 3-butanedione monoxime (BMD) on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls. Physiol. Plant. 130, 613–626 (2007)
Sievers, A., Buchen, B., Volkmann, D., Hejnowicz, Z.: Role of the cytoskeleton in gravity perception. In: Lloyd, C.W. (ed.) The Cytoskeletal Basis of Plant Growth and Form, pp. 169–182. Academic, London (1991)
Švegždienė, D., Raklevičienė, D., Koryznienė, D.: Gravisensing in hypocotyls and roots of garden cress seedlings. Biologija (Vilnius) 53(2), 63–66 (2007)
Todd, P.: Mechanical analysis of statolith action in roots and rhizoids. Adv. Space Res. 14(8), 121–124 (1994)
Vitha, S., Yang, M., Sack, F.D., Kiss, J.Z.: Gravitropism in the starch excess mutant of Arabidopsis thaliana. Am. J. Bot. 94(4), 590–598 (2007)
Volkmann, D., Baluška, F.: Actin cytoskeleton related to gravisensing in higher plants. In: Staiger, C.J., Baluška, F., Volkmann, D. (eds.) Actin: A Dynamic Framework for Multiple Cell Functions, pp. 557–571. Kluwer, Norwell (2000)
Volkmann, D., Buchen, B., Hejnowicz, Z., Tewinkel, M., Sievers, A.: Oriented movement of statoliths studied in a reduced gravitational field during parabolic flights of rockets. Planta 185, 153–161 (1991)
Volkmann, D., Winn-Borner, U., Waberzeck, K.: Graviresponsiveness of cress seedlings and structural status of presumptive statocytes from hypocotyls. Plant Physiol. 142, 710–716 (1993)
Yoder, T.L., Zheng, H.Q., Todd, P., Staehelin, L.A.: Amiloplast sedimentation dynamics in maize columella cells support a new model for the gravity—sensing apparatus of roots. Plant Physiol. 125, 1045–1060 (2001)
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Švegždienė, D., Koryznienė, D. & Raklevičienė, D. Comparison Study of Gravity-Dependent Displacement of Amyloplasts in Statocytes of Cress Roots and Hypocotyls. Microgravity Sci. Technol. 23, 235–241 (2011). https://doi.org/10.1007/s12217-010-9190-0
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DOI: https://doi.org/10.1007/s12217-010-9190-0