Abstract
Demand for potato is steadily rising in developing countries, where actual per-hectare production levels reach mere fractions of the yields achieved in Europe or North America. Improving abiotic stress tolerance, e.g., against drought, could increase these low potato yields and thus help to satisfy the growing demand for this crop. Hypotheses about genes and traits that could mitigate yield decreases caused by drought have been driven by information obtained from model plants and have recently been complemented with data of high throughput gene expression profiling and metabolite studies on potato genotypes under water stress. Principal tolerance traits that could diminish the vulnerability of potato yields to drought stress include improved detoxification of reactive oxygen species produced during stress, optimized stomatal control under drought to reduce water loss but at the same time allow for continuous CO2 access for photosynthesis, and mechanisms to protect proteins and membranes from damage by water stress. Candidate genes underlying these traits as well as genotypes that express them are available and, after appropriate validation, could be used for breeding.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
On a global scale, demand for potato (Solanum tuberosum L.) is steadily rising. Slight decreases in potato consumption in developed countries are overcompensated by strong increases in developing countries, where potato production has tripled between 1960 and 2000 and will need to grow continuously to satisfy unremitting demand [16, 29]. Most of the production increase will have to come from yield increases as new cultivable area is scarce. Many potato production areas in developing countries are located in semi-arid areas, where drought spells contribute to large harvest losses. In these regions, where the yields range around 30% of the global mean, adapted drought-tolerant potato varieties could improve yield considerably.
The critical level of soil moisture tension for potato yield and quality ranges around −0.7 bar [76]. Average tuber yield reduction per mm water deficit has been estimated at 117 kg/ha [107]. Nevertheless, potato water productivity on dry weight basis is two times higher that of wheat or maize and three times higher than that of rice [28, 103]. Water stress at virtually any stage of potato development decreases yield, but, depending on the timing, drought may have different effects on plant growth and tuber yield. Drought at early developmental stages, before tuberization, reduces the stolon number per stem without affecting the number of tubers initiated per stolon [41]. It was suggested that drought during tuberization lowers tuber number, and during bulking it lowers both tuber number and size [70]. In field trials tuber yield reduction was greatest either under drought during tuber initiation [23, 55, 68] or during tuber bulking [63, 64], probably due to differences in phenology of the tested genotypes. Besides yield, drought may also affect tuber quality. Water stress causes an increase of glycoalkaloid contents, especially of α-solanine and α-chaconine in many varieties [14]; moreover, water stress might cause tuber defects such as cracking, secondary growth, malformations, hollow heart, and internal brown spot [69].
Tuber yield depends primarily on the amount of radiation intercepted by the plant and on water availability [3]. Plants with a large canopy intercept more light, but also have a larger surface exposed to transpiration. Drought generally reduces canopy size [24, 102]. Leaf growth stops at a certain threshold of transpirable soil water. These threshold values range between 40% and 50% for many crops, but in potato, leaf growth ceases when the fraction of transpirable soil water declines to only 60%, illustrating the susceptibility of potato to water stress [111]. Reduction of canopy size helps the plant diminish the evaporative demand and improves plant survival under water stress, but, on the other hand, results in yield decreases due to the smaller leaf area available for photosynthesis. Early leaf appearance combined with the ability to sustain leaf growth with increasing soil moisture deficit would improve productivity in the presence of drought [48]. Deeper and denser roots could improve water availability to the plant and contribute sustaining leaf growth and plant productivity under drought, where water still remains available in deeper soil layers. Tuber yield, reduction of stomatal conductance, photosynthesis, and leaf area are significantly correlated to root dry mass under stressed conditions. Consequently, root mass in the plow layer could be used as a selection criterion for enhancing tolerance of potato to drought [44, 56]. A good indicator of drought adaptation is increased root-shoot ratio under water stress [13, 40, 46].
Drought Tolerance Breeding in Potato
Available variation of drought tolerance and water use efficiency in potato germplasm opens the path to enhance yields under water-limiting conditions by breeding, but the success of this approach has been limited by both the inconsistency of natural drought events and the complexity of plant drought stress responses. Selecting for drought tolerance under natural conditions is challenging, as the occurrence of stress is unpredictable due to day-to-day and year-to-year variation of climate. Different stress intensities and timing from one year to the next can lead to elicitation of distinct drought responses in different selection cycles, resulting in limited breeding progress. Therefore, in early breeding stages, breeders often prefer to select for high yield in favorable environments and expect that this will also increase yields under abiotic stress conditions. This might be true for environments with weak stress, but if the target environment is commonly affected by severe stress, selection gains in an unstressed target environment are of little or no help in improving yields in stress environments. Finally, when genotypes are selected mainly under favorable conditions, much useful genetic variation for stress tolerance may be lost [7]. In addition, direct selection for drought tolerance under water-stressed conditions is hampered by low heritability, polygenic control, and epistasis of many drought tolerance traits.
Breeding to reduce the gap between yield potential and yield in drought-prone environments requires highly productive water-stress-tolerant parental material that expresses tolerance traits likely to improve drought tolerance without diminishing yield potential. Not surprisingly, greatest tolerance levels have been found in Andean native potato and wild tuber bearing potato species [5, 87] that evolved in harsh environments at high altitudes above 3,000 masl and were regularly exposed to drought during their evolution. However, native Andean potato genotypes generally have low yields and are not adapted for most commercial potato production systems, and wild potato species harbor many agronomically unfavorable traits. Hybridizing adapted with non-adapted material usually conserves chromosomal regions containing large amounts of undesirable genes. Linkage of undesirable genes with favorable gene combinations may need a large number of sexual crosses to separate and therefore require considerable time and effort to recover previous yield levels. Consequently, breeders are reluctant to introduce unadapted parents such as wild potato species into breeding programs, unless they can target a specific trait from the unadapted parent. Therefore, successful breeding requires exact information on effective tolerance traits, their heritability and their genotype-by-environment interaction, plus suitable selection tools for the traits of interest.
According to Levitt [58], drought resistance can be based on (1) drought stress tolerance, (2) drought stress avoidance, (3) drought escape, or (4) on a combination of these mechanisms. Drought escape means that the plant life cycle is complete before drought stress inhibits further plant growth. Indeed, Levy [60] observed that early-maturing potato varieties had lower yield losses under drought than later maturing ones, but this finding is valid only for late-season drought events. Drought stress tolerance implies that plants are tolerant to desiccation to some extent, and that moderate short-term disturbances of plant water balance do not immediately affect yield. This could be accomplished by protecting cell components from the adverse effects of water loss through expression of cell rescue mechanisms and through increased capacity of leaves to recover after wilting. Drought stress avoidance has two components: reduction of water loss and improvement of water uptake. While improvement of water uptake allows the plant to meet transpirational demand under lower soil water contents, increasing evaporation resistance by closing stomata or decreasing the evaporative surface, i.e., leaf area, reduces transpiration and water loss. Crop yield is intrinsically linked to transpiration, and reduced transpiration generally lowers biomass accumulation. Therefore, selection for drought stress avoidance genotypes with low transpiration generally results in diminished yields under optimum conditions [93]. Increasing the amount of harvested biomass per unit of transpired water, i.e., water use efficiency (WUE), would be an option to mitigate the yield penalty caused by reduced transpiration. Favorable leaf anatomy as well as optimal regulation of stomatal and mesophyll conductance for continued CO2 diffusion to the chloroplasts and increased Calvin cycle activities might increase intrinsic WUE, while improvement of harvest index under water stress would contribute to increased crop WUE. Plants with high WUE would be highly desirable for irrigated potato cultivation in regions where water resources are scarce. For areas with frequent drought spells, in addition to increased WUE, drought tolerance traits that help the plants survive and produce under water stress would be highly beneficial.
Potato breeding for tropical environments presents a particular challenge. Temperatures in the lowland tropics are often too high, irradiance excessive, and precipitation insufficient to sustain potato growth. So in addition to drought tolerance, heat tolerance is an important breeding target for potato improvement for tropical areas. The combined effect of drought and heat stress differs markedly from that of individual drought or heat stress [81, 82]; consequently, many drought tolerance traits might not be effective when a combination of drought and heat affects the plant.
Drought Stress Signaling in Plants
Drought stress activates a wide array of responses in plants, which together with constitutive traits determine whether a plant is more tolerant or susceptible to drought. About 10% of the transcriptome is differentially regulated under drought [18, 79, 97]. The number of genes implicated in yield maintenance under drought might be nearly as large as the number of genes implicated with yield in general.
How plants perceive water stress is not yet clearly defined. A general model describing plant responses to stress starts with the perception of signals from the environment, followed by the generation of second messengers such as inositol phosphates, phospholipids, and reactive oxygen species. In yeast, an osmotic stress-sensing pathway involving receptor kinases perceives osmotic stress at the membrane level and transmits the signal to MAP-kinases [38]. Putative orthologs to osmotic stress-sensing kinases of yeast have been found in plants [21, 105], where it is probable that a multitude of sensors is involved in water stress perception and signal transduction, triggering an array of responses through secondary messengers such as hormones, phospholipids, calcium ions, and other signals. The different water stress signaling pathways may interact with one another using shared components generating an intertwined network (reviewed by Wu et al. [112]).
For potato it was conclusively shown that roots sense soil water deficits well before any loss of turgor occurs [62]. In response to slight water potential decreases in roots, abscisic acid (ABA) is produced in root tips and is subsequently transported through the xylem to leaves and causes growth attenuation and stomatal closing well before drops in leaf water content or water potential appear in this organ [62]. ABA accumulation in the xylem then triggers an array of reactions, including liberation of ABA from sugar complexes, leaf and shoot growth attenuation, transcriptional induction of a battery of genes that presumably protect the cells from damage under prolonged stress, as well as more general adaptive shoot and root responses (reviewed by Wasilevska et al. [109]. At least four independent regulatory systems for gene expression changes in response to water stress have been identified, two are ABA-dependent and two are ABA-independent [91].
Drought Stress Tolerance Candidate Genes and Traits in Potato
Microarray-based gene expression profiling in drought-exposed plants revealed a large number of drought-induced and repressed genes that inform us about plant stress responses at molecular level [18, 79]. During recent years, results of several microarray experiments assessing gene expression changes in drought-stressed plants also became available for potato. Most analyses were targeted towards leaves; only one study was addressed toward tubers and leaves [110]. Up to now, no molecular data on root or stolon responses of potato to drought have been published.
Drought adaptation and acclimation responses on gene transcriptional level in native Andean potato derived from true seed were described in Watkinson et al. [110]. Schafleitner et al. [87], Vasquez-Robinet et al. [106] and Mane et al. [87] reported gene expression changes and metabolite accumulation in native Andean potato clones exposed to prolonged drought stress. In most studies the TIGR 10 k potato cDNA microarray was used [81]. Mane et al. [87] applied the POCI 44 k oligonucleotide chip [53]. In the studies on clonal material, clone Sullu was used as the drought-tolerant genotype and drought susceptible controls were either SA2563, Ccompis, or Negra Ojosa. Each of these articles includes lists of drought-induced and repressed genes of potato. Additionally, the gene expression data obtained with the TIGR 10 K are publically available in the Solanaceae Gene Expression Database (http://www.jcvi.org/cgi-bin/potato/study/sol_study.pl).
Together with agronomic, physiological, and biochemical assessments, gene expression data have given valuable insight into drought responses of different potato genotypes with different drought susceptibility levels. The tolerance traits suggested by gene expression profiling include features involved with signaling; transcription regulation; photosynthesis; carbohydrate, amino acid, and lipid metabolism and transport; hormone biosynthesis, degradation and transport; and cell rescue. However, microarray studies typically are performed on a small number of clones and, in spite of thorough statistical analysis, are prone to errors resulting in false positive candidate genes, and, more importantly, might fail to pinpoint important tolerance genes. Furthermore, it might be difficult to discern whether up-regulation of a specific gene points towards its role in a tolerance mechanism or transcriptional induction merely reflects a stress response without impact on drought tolerance. The microarray studies on drought stress responses in potato were carried out comparing expression patterns in tolerant and susceptible genotypes, which facilitates the discrimination of putative tolerance genes from stress-responsive genes without a role in drought tolerance. Verifying the expression patterns of candidate genes over a larger number of accessions with varying tolerance would help to confirm the value of putative candidate genes. Coupling gene expression studies with genetic linkage analysis, where expression levels are treated as quantitative phenotypes, would be another means to validate the impact of gene expression changes on the drought tolerance phenotype, provided that gene expression differences between stressed and control plants are heritable [61]. But none of these approaches substitutes the experimental proof that a candidate gene indeed provides improved tolerance. Testing the large number of candidate genes by transgenic over-expression or gene silencing and phenotyping of the resultant transgenic plants would be a very laborious task. Model plants such as Arabidopsis thaliana mutants or knock-out lines are very helpful for candidate gene validation, but the effect of a specific candidate trait on tuber yield maintenance can only be accomplished in potato, as the popular model plants do not produce tubers. Furthermore, a specific candidate tolerance gene might interact with an array of other genes and thus is only operational in a specific genetic background. In spite of all these limitations, gene expression studies are apt to identify candidate genes for tolerance and allow establishing hypotheses on pathways conferring drought tolerance in plants.
Genes Involved with Drought Stress Signaling in Potato
Little is known about signaling factors involved with water stress tolerance in potato. Gene expression measurement revealed an array of up-regulated genes functioning in signaling such as Ca2+-binding and GTP-binding factors, kinases, and phosphatases, but no studies have been performed in potato to confirm the involvement of these factors in tolerance mechanisms. Microarray gene expression analysis pinpointed a protein phosphatase 2C gene of potato, an ortholog to ABI2 of A. thaliana, that is involved with ABA sensing and signaling [36]. High expression of this gene was correlated with yield maintenance under field conditions [86]. Inositol phosphates and their turnover products have been implicated to play important roles in stress signaling. Constitutive transgenic expression of an Arabidopsis inositol polyphosphate 6-/3-kinase in tobacco improved tolerance to diverse abiotic stresses [116]. An ortholog to the inositol polyphosphate kinase is present and expressed in potato, but its implication in abiotic stress signaling has not yet been addressed. Inositol polyphosphate kinase was expressed, but was not significantly up-regulated in potato under prolonged drought stress. The conducted microarray studies assessed drought stress responses taking place several days and weeks after drought stress onset, and therefore fail to identify early signaling events water stress.
Phytohormones play a key role in drought stress signaling. While ABA-responsive genes were found induced in drought-stressed potato, no ABA-metabolizing genes were differentially regulated under prolonged water stress. In contrast, transcripts encoding gibberellin-degrading enzymes accumulated under drought [87]. Gibberellins act as growth promoters and increased degradation of this hormone might contribute with decreased shoot and leaf growth under drought. Increased ethylene biosynthesis gene expression, which was observed predominantly in drought-susceptible potato, might be associated with increased stress perception. Ethylene, together with ABA, is involved with leaf growth attenuation under water stress [92].
Transcription Factors Involved in Drought Signaling and Response
In potato, differential regulation of several transcription factor families was found under drought conditions. Evidence for the action of some of the water-stress inducible transcription factors of potato such as MYC, MYB, Nam, NF-Y, DREB, GAI and ASR family members is available from other plant systems [1, 2, 43, 77, 84]. High expression of a DREB transcription factor in potato, an ortholog to DREB1D of A. thaliana, was associated with increased drought tolerance in a trial on 16 improved potato varieties and breeding clones [86]. The abscisic acid, stress ripening gene (ASR) family comprises at least four genes in Solanaceae [30]. The first identified drought-induced gene of potato, DS2, belongs to this gene family [26]. The best-characterized gene family member, ASR1, as well as ASR2 are strongly up-regulated in potato under drought [87]. ASR1 has a dual function: in the nucleus it acts as a transcription factor and regulates the expression of a hexose transporter, while in the cytosol it functions as chaperone and stabilizes proteins under abiotic stress conditions [117]. Further transcription factor families found up-regulated in drought-tolerant potato comprised GATA, bZIP, WRKY1, and TAF-3 transcription factors, suggesting a role for these factors in the establishment of drought tolerance in this crop [87, 106, 110].
Recently, CSPA and CSPB, bacterial RNA chaperones that resolve RNAs that have been kinetically trapped in miss-folded form, were shown to promote stress adaptation in multiple plant species [20]. RNA chaperones are ubiquitous proteins and homologs of CSPA were found in wheat [51] and A. thaliana [52]. In potato, a putative homolog to CSPA was slightly induced under drought. CSP-like genes might merit a more thorough investigation of their role in stress tolerance of potato and of their use in crop improvement.
Photosynthesis and Respiration
Dependending on the kind and timing of drought, reduced leaf area may affect net photosynthesis more than inhibition of photosynthesis itself. Nevertheless, maintenance of photosynthetic activity under water stress and recovery from drought-stress associated drop of photosynthetic activity is a key element of plant drought tolerance. Under drought, photosynthetic efficiency decreases and CO2 assimilation is greatly reduced in susceptible genotypes, while resistant genotypes can continue with photosynthesis for weeks after drought onset [110], indicating that reduction of photosynthetic activity under drought is highly genotype-dependent. Photosynthesis in water-stressed plants may be restricted by stomatal limitations, i.e., in how much CO2 remains available for the photosynthetic apparatus, when stomatal conductance is kept low to avoid excessive transpiration. There may also be non-stomatal limitations, such as metabolic impairment including reduced ribulose bisphosphate carboxylase regeneration and ATP synthesis that inflict carbon assimilation under drought. Mesophyll conductance for CO2 also may change dramatically under drought and, together with stomatal closure, limit the access of CO2 to the chloroplasts (reviewed by Warren [108]). Under moderate drought, stomatal limitations prevail, while under extreme drought stress metabolic impairment may occur.
Stomatal conductance declines faster than photosynthetic capacity. Liu et al. [62] observed that photosynthesis did not significantly decrease when stomatal conductance decreased from 0.7 to ca. 0.4 mol m−2 s−1, while the relationship between photosynthetic efficiency and stomatal conductance increased approximately 1.7-fold, demonstrating that photosynthetic WUE of potato increases at moderate soil water deficits by partial stomatal closure. Variation in stomatal density has been observed between different potato varieties and low stomatal density was proposed to be associated with drought tolerance in potato [10]. Masle et al. [71] reported the isolation of the ERECTA gene of A. thaliana, a leucine-rich repeat, receptor-like kinase that controls transpiration efficiency and has effects on stomatal density, epidermal cell expansion, mesophyll cell proliferation and cell–cell contact. It remains to be tested whether genotypic variation of the potato ortholog to this gene has an impact on stomatal density and transpiration efficiency in potato. Decreased stomatal density was also observed in potato transformed with trehalose-6-phosphate synthase from yeast [94]. These plants showed retarded wilting of excised leaves and lower CO2 assimilation.
On the gene expression level, photosynthesis-related genes such as chlorophyll-binding proteins or photosystem components are up-regulated under drought in resistant genotypes and down-regulated in susceptible genotypes [87]. The significance of the differential expression of these genes for tolerance is unclear. During recovery from drought events, resistant accessions induce genes involved with thylakoid-associated processes, including genes encoding photosystem I and II proteins and chloroplast envelope protein genes, ribulose bisphosphate carboxylase, thioredoxin-responsive plastidic glyceraldehyde-3-phosphate dehydrogenase and fructose-1,6-bisphosphatase genes, and enzymes of photosynthetic carbon fixation [67]. These gene expression changes indicate that photosynthetic recovery might require de novo synthesis of photosynthetic proteins.
Reactive Oxygen Species
Under stress, the photosynthetic machinery, like other cell compartments, is affected by increased concentrations of reactive oxygen species (ROS) [88]. The primary sources of ROS in chloroplasts are the Mehler reaction and the antenna pigments [6]. Other sources of ROS in plant cells under stress include the photorespiratory pathway in peroxisomes, detoxifying reactions catalyzed by cytochromes in both the cytoplasm and the endoplasmic reticulum, and oxidative processes in mitochondria. The high energy consumption of the plant’s stress response increases respiration rate with a linked production of ROS [101]. ROS are toxic to cells as they can cause oxidative damage to proteins, DNA, and lipids. On the other hand, ROS act also as signaling molecules for regulating development and various physiological responses [75]. How plants control this dual role is largely unknown, but it is clear that the steady-state level of ROS in cells needs to be tightly regulated. Major ROS-scavenging enzymes in chloroplasts include superoxide dismutase, ascorbate peroxidase, catalase, glutathione peroxidase, and peroxiredoxin. Members of all these gene families were up-regulated in tolerant potato under drought [67, 87, 106]. CDSP 32 and CDSP34 are other chloroplast-localized proteins involved with ROS detoxification [19, 57]. Additionally glutathione synthetase, glutathione-S-transferase, glutathione transporter, as well as two thioredoxins were induced in drought tolerant Sullu, pinpointing the increased capacity of ROS detoxification of this clone. During drought recovery, four thioredoxin-related chloroplast targeted genes were induced in a drought-resistant accession only.
Mitochondria are other targets of oxidative stress, as the interaction of O2 with reduced components of the electron transport chain and fatty acid peroxidation leads to ROS formation. Under drought, we observed up-regulation of an antiquitin family aldehyde dehydrogenase 7 gene previously suggested to be involved in the detoxification of reactive aldehyde species generated by oxidative stress-associated lipid peroxidation [83], which is a good candidate for mitigating oxidative stress in mitochondria. Another important drought-inducible mitochondrial function concerns alternative oxidase 1a, which also is strongly upregulated in potato under drought. Respiration is generally enhanced by drought, the drought-induced increase in oxygen uptake being due mostly to a twofold increase in the capacity of the alternative oxidase pathway. This pathway has been shown to play a broad role in determining the normal redox balance in the cell and is important in maintaining photosynthetic electron transport under stress, either through direct consumption of reducing power, through sustained production of CO2, or by allowing other power-consuming processes (e.g., photorespiration) to operate [35]. An increased respiration rate seems necessary for photosynthesis recovery after a period of water stress. This might be the reason genes encoding mitochondrial proteins such as carrier proteins, ubiquinol-cytochrome c reductase and an epsilon subunit of mitochondrial F1-ATPase are up-regulated during drought recovery in tolerant potato [67].
The up-regulation of ROS detoxifying enzyme genes and the accumulation of antioxidants in tolerant varieties point towards a crucial function of ROS detoxification in drought tolerance. Transgenic over-expression of nucleoside dikinase 2, Cu/Zn superoxide dismutase, and ascorbate peroxidase, which neutralize ROS-mitigated deleterious effects of free oxygen radicals, improves drought tolerance and thus provides further evidence for the importance of ROS detoxification in water stress tolerance [73, 98, 99]. Therefore, genes that have the potential to protect the cell from oxidative stress are good candidates for drought tolerance genes.
Changes in Carbohydrate Flux Under Drought
It has been noted that water stress causes changes in the concentration of numerous metabolites in plants. In drought-stressed potato, alterations in metabolite steady state levels, as well as in metabolite flux, have been noted in tubers and leaves. Under water stress, sucrose supply to tubers is reduced and tuber growth and starch biosynthesis is repressed. While in turgid tubers, starch biosynthesis is favored and sucrose phosphate synthase is inhibited, mild water deficits lead to a rapid switch from starch biosynthesis and net sucrose degradation to activation of sucrose phosphate synthase and net sucrose synthesis [33]. The shift from starch to sucrose resynthesis is controlled by redox-dependent, trehalose-6-phosphate mediated activation of ADP-glucose pyrophosphorylase [54].
A shift in carbohydrate partition towards tubers has been observed under laboratory drought conditions [34], while increased harvest index appears to be genotype dependent under field drought conditions. The ability to increase dry matter allocation to tubers might depend on the increased sucrose phosphate synthase-mediated sucrose resynthesis under water stress that might attract water to tubers and sustain growth during water deficits [34].
More candidate genes for improving harvest index under drought have been proposed, e.g., SNF1-related protein kinase, which plays a key role in carbon partition [66], but more research is needed to identify factors that promote preferred carbon allocation in tubers during water stress under field conditions.
Osmotic Adjustment
A net increase of solute concentrations in plants in response to a fall in the water potential of the cell’s environment results in a decrease of the osmotic potential in plant cells, which in turn attracts water into the cell to maintain turgor pressure. Osmotic adjustment often is mentioned as a desirable trait in plant drought tolerance [42, 47, 59]. It takes place in leaf, root, and tuber tissue of drought-tolerant and -susceptible potato genotypes. While Jefferies [47] reports osmotic adjustment in leaves in ranges between 0.01 and 0.16 MPa, Coleman [22] measured a maximum value for osmotic adjustment of 0.54 MPa in young expanding leaves of an improved potato variety. The difference in the osmotic adjustment between these two reports is probably due to the low osmotic potential in the control plants used by Jefferies [47]. Basu et al. [12] observed a decrease in water potential from −0.2 to −1.0 MPa in potato under drought. These data demonstrate that osmotic adjustment depends on the kind and strength of stress as well as of the potato genotype. As demonstrated by Serraj and Sinclair [89], the contribution of osmotic adjustment to increased water uptake from drying soils is very limited, therefore osmotic adjustment might be more important for protecting proteins and membranes (reviewed by Yancey [115]) than as a means to acquire more water. The value of osmotic adjustment as a drought tolerance trait is controversial since turgor maintenance could result in maintenance of green leaf area and delay the activation of water-conserving mechanisms such as stomatal closure and leaf abscission, thus exposing the plant to even stronger stress when drought prevails [89]. However, under transient water stress, turgor maintenance may have a function in sustaining tissue metabolic activity. Osmotic adjustment does not take place homogenously in the whole plant. Instead, active roots and young leaves show greatest decreases in osmotic potential. Therefore osmotic adjustment might favor turgor maintenance particularly in young and essential parts of the plant and thus improve plant survival, regeneration, and productivity under drought.
A large array of metabolites has been proposed to contribute to osmotic adjustment in potato, including all kinds of plant-compatible solutes, such as metal ions, organic acids, polyamines, sugars, sugar alcohols, amino acids and their derivates, and proteins [39]. Proline was proposed to play a key role in osmotic adjustment, as its concentration in plants strongly increases under water stress [25]. In drought-stressed potato, genes of proline biosynthesis such as Δ1-pyrroline 5 carboxylate synthase and Δ1-pyrroline 5 carboxylate dehydrogenase were found induced, but no correlation between proline content and drought tolerance could be established [11, 85]. It was proposed that proline accumulation is more an indicator of injury by drought than a tolerance factor [8, 72]. However, there is evidence that proline biosynthesis intermediates act in drought stress signaling [45]. Amino acids other than proline did not accumulate in the drought-tolerant landrace Sullu under stress, but concentration of amines such as ethanolamine and putrescine was increased [67]. Organic acid content was much higher in Sullu than in a susceptible control, with significant drought-mediated increases of high basal concentrations of caffeic acid, a putative strong antioxidant and chlorogenic acid precursor [4, 114], and citric acid [106]. Sucrose accumulation in leaves of tolerant potato was inconsistent between experiments, ranging from no accumulation and even slight decline in resistant Sullu [87] to very high sucrose concentrations in drought-stressed leaves of the same clone [67]. Similarly, trehalose accumulation in Sullu varied between different drought trials from no detected changes to accumulation of moderate levels [106]. Further osmolytes accumulating in drought-tolerant potato that are considered to be involved with drought tolerance in plants are galactinol, raffinose, pinitol, and inositol [67, 87, 90, 95, 97, 106]. Sugar alcohols also may act as antioxidants in stressed plant cells. Corresponding biosynthesis genes were found up-regulated under stress [67, 87].
Stabilization of Proteins and Membranes
An array of genes encoding proteins putatively involved with maintaining the hydration of cellular compounds such as proteins and membranes are up-regulated under water stress. Several genes encoding late embryo abundant (LEA) and dehydrin genes, particularly LEA5 and LEA 18, were induced in potato under drought [87]. These proteins prevent protein aggregation under stress and thus could be of use for breeding drought-tolerant crops [9].
Protein chaperones of the DnaJ family as well as an array of heat shock proteins (HSP) have similar roles as LEA proteins and their expression is also increased under drought stress in potato [87, 106, 110]. In vitro studies have shown that HSPs can bind selectively to denatured proteins, prevent their aggregation, and maintain them in a competent state for refolding by other chaperones [31]. DnaJ-like proteins belong to a large and diverse family of chaperones. Drought-induced expression of these genes has been observed in many plant species [74] and might contribute to plant performance under water stress. Expression of both gene families was induced in potato under drought; however, while HSP genes were up-regulated in both drought-tolerant and -susceptible cultivars, DnaJ-like protein genes were much stronger expressed in tolerant potato [87, 106]. One of the drought-induced potato HSPs is an ortholog to the Arabidopsis HSP At5g12030 that previously has been shown to enhance drought tolerance [96]. Furthermore, an ATP-dependent metalloprotease and chaperone found induced in potato has been associated with drought tolerance [27].
The cell and organelle membranes are susceptible to injury by water stress. Limiting membrane damage during desiccation and membrane regeneration are critical features of tolerance. The extent of membrane damage is commonly used as a measure of tolerance to drought [15]. In potato, the ability of the adapted cells to maintain cellular and sub-cellular membrane integrity under conditions of severe water stress was found to be associated with a reduced level of unsaturated fatty acids in the membrane lipids [37]. Expression analysis pointed to non-specific lipid transfer proteins that are highly induced in tolerant potato [87]. Further studies will be required to assess membrane-stabilizing and regenerating processes in drought tolerant potato.
Future Directions
The present knowledge on the physiological and genetic basis of water stress tolerance in other crops as well as in potato helps us to better understand the drought tolerance trait network. For some of the proposed traits, such as increased root mass and enhanced ROS detoxification, strong evidence for their beneficial effect in drought tolerance is already available, while for other candidate traits, such as osmotic adjustment or protein and membrane stabilization-related mechanisms, direct proof for their function in yield maintenance under stress in potato is still missing. To determine the functionality of candidate genes and traits, thorough agronomical, physiological, and genetic analysis of candidate traits will be required in germplasm panels or segregating populations. One way to speed up candidate gene validation and efficiently narrow down the number of candidate genes obtained in microarray studies could be accomplished by quantitative trait loci (QTL) analysis of specific drought tolerance traits, similar to that described by Gebhardt et al. [32]. For this purpose, publicly available databases of potato gene sequences, e.g., the TIGR Potato Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=potato), could be mined for single nucleotide polymorphisms (SNPs) in a number of candidate genes that are presumably evenly spread over the potato genome. Cost-effective methods such as high resolution melting would allow screening of large germplsm panels or populations for SNPs in candidate genes [113]. The resulting candidate gene-based markers could be used together with available anonymous markers, such microsatellite markers that would help to identify homologous groups, for a QTL approach or association genetics analysis to identify candidate gene loci that are co-localized with QTLs for specific drought tolerance traits. Chromosomal regions explaining diversity of a specific trait have been successfully identified in tetraploid potato [17, 65, 80]. Examples of drought tolerance traits that have been targeted in QTL studies using anonymous markers in crops were WUE [50], osmotic adjustment [100], delayed senescence [49], deep rooting [78], or cell membrane stability under drought stress [104]. Using markers derived from candidate genes instead of anonymous markers could indicate links between genes and QTLs and thus pinpoint involvement of candidate genes with specific tolerance traits. The alleles associated with a specific drought parameter might offer a favorable allele for this trait and can be used for marker-assisted selection in breeding. Although this approach does not provide direct evidence that the candidate gene marker linked with the QTL is indeed the gene underlying this QTL, this method can strengthen hypotheses on gene functions in specific drought tolerance traits and at the same time yields gene markers linked to traits of interest.
For many traits, a strong genotype by environment interaction might limit their use to improve drought tolerance over a broad range of environments; therefore it is very improbable that one single tolerant potato variety can be successful in all drought environments. However, it can be assumed that ROS detoxification, which provides benefits under many stress conditions, could be a suitable drought tolerance trait for a broad range of environments. In contrast, larger roots or high osmotic adjustment may be useful only for specific drought environments, either for situations where water remains available in deeper soil layers or in cases where regeneration after drought periods is crucial for yield maintenance. For breeding well-adapted potato that yields well in a specific drought environment, we need deeper insight into the potential impacts of different tolerance traits on yield maintenance in the target environment. For this purpose, materials expressing different drought tolerance traits need to be tested in multi-location trials to filter out tolerance traits and trait combinations that are effective either in a broad or narrow range of environments. Systematic characterization of soil and climate variables of target environments parallel to trait capture and validation would facilitate identifying the best trait options for each environment and allow better targeting of tolerance traits to specific drought environments.
Abbreviations
- ABA:
-
Abscisic acid
- HSP:
-
Heat-shock protein
- LEA:
-
Late embryo abundant
- masl:
-
Meters above sea level
- ROS:
-
Reactive oxygen species
- SNP:
-
Single nucleotide polymorphism
- WUE:
-
Water use efficiency
References
Abe H, Yamaguchi-Shinozaki K, Urao T et al (1997) Role of Arabidopsis MYC and MYB homologs in drought and abscisic acid-regulated gene expression. Plant Cell 9:1859–1868
Achard P, Cheng H, De Grauwe L et al (2006) Integration of plant responses to environmentally activated phytohormonal signals. Science 311:91–94
Allen EJ, Scott RK (1980) An analysis of growth of the potato crop. J Agr Sci 94:583–606
Andre CM, Oufir M, Guignard C et al (2007) Antioxidant profiling of native Andean potato tubers (Solanum tuberosum L.) reveals cultivars with high levels of β-carotene, α-tocopherol, chlorogenic acid, and petanin. J Agric Food Chem 55:10839–10849
Arvin MJ, Donnelly DJ (2008) Screening potato cultivars and wild species to abiotic stresses using an electrolyte leakage bioassay. J Agric Sci Techn 10:33–42
Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ et al (eds) Photoinhibition (Topics in Photosynthesis), vol 9. Elsevier, Amsterdam, pp 227–287
Baenziger M, Edmeades GO, Beck D, Bellon M (2000) Breeding for drought and nitrogen stress tolerance in maize: from theory to practice. CIMMYT, Mexico
Balasimha D (1983) Proline accumulation in water stressed potatoes. J Indian Potato Assoc 10:56–59
Ban QY, Wang YC, Yang CP et al (2008) LEA genes and drought tolerance. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 3:6
Bansal KC, Nagarajan S, Sukumaran NP (1982) Stomal density and size in some potato varieties. J Indian Potato Assoc 9:33–36
Bansal KC, Nagarajan S (1986) Leaf water content, stomatal conductance and proline accumulation in leaves of potato (Solanum tuberosum L.) in response to water stress. Indian J Plant Physiol 29:397–404
Basu PS, Ashoo S, Sukumaran NP (1998) Changes in net photosynthetic rate and chlorophyll fluorescence in potato leaves induced by water stress. Photosynthetica 35:13–19
Begg JE, Turner NC (1976) Crop water deficits. Adv Agron 28:161–217
Bejarano L, Mignolet E, Devaux A et al (2000) Glycoalkaloids in potato tubers: the effect of variety and drought stress on the α-solanine and α-chaconine contents of potatoes. J Sci Food Agric 80:2096–2100
Blum A, Ebercon A (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci 21:43–47
Bowen WT (2003) Water productivity in potato production. In: Kijne JW, Barker R, Molden D (eds) Water productivity in agriculture: limits and opportunities for improvement. CABI Publishing, CAB International, Wallingford, pp 229–238
Bradshaw JE, Hackett CA, Pande B et al (2008) QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum). TAG 116:193–211
Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. J Exp Bot 55:2331–2341
Broin M, Cuiné S, Eymery F et al (2002) The plastidic 2-cysteine peroxiredoxin is a target for a thioredoxin involved in the protection of the photosynthetic apparatus against oxidative damage. Plant Cell 14:1417–1432
Castiglioni P, Warner D, Bensen RJ (2008) Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions. Plant Physiol 147:446–455
Chefdor F, Bénédetti H, Depierreux C et al (2006) Osmotic stress sensing in Populus: components identification of a phosphorelay system. FEBS Lett 580:77–81
Coleman WK (2008) Evaluation of wild Solanum species for drought resistance: 1. Solanum gandarillasii Cardenas. Envir Exp Bot 62:221–230
dalla Costa L, delle Vedove G, Gianquinto G et al (1997) Yield, water use efficiency and nitrogen uptake in potato: influence of drought stress. Potato Res 40:19–34
Deblonde PMK, Ledent JF (2001) Effects of moderate drought conditions on green leaf number, stem height, leaf length and tuber yield of potato cultivars. Europ J Agron 14:31–41
Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223
Dóczi R, Kondrák M, Kovács G et al (2005) Conservation of the drought-inducible DS2 genes and divergences from their ASR paralogues in solanaceous species. Plant Physiol Biochem 43:269–276
Fan M, Jin LP, Huang SW et al (2007) Cloning and expression of a full-length cDNA of SoFtsH gene in potato under drought stress. Acta Agron Sinica 33:1748–1754
FAO 2003. Unlocking the water potential of agriculture. ISBN: 9251049114, http://www.fao.org/DOCREP/006/Y4525E/Y4525E00.HTM, Cited 15 Feb. 2009
FAO 2008. Potato world. http://www.potato2008.org/en/world/index.html, Cited 15 Feb. 2009
Frankel N, Carrari F, Hasson E, Iusem ND (2006) Evolutionary history of the Asr gene family. Gene 378:74–83
Garrett JL, Vierling E (2000) A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein. Plant Physiol 122:189–198
Gebhardt C, Li L, Pajerowska-Mukthar K et al (2007) Candidate gene approach to identify genes underlying quantitative traits and develop diagnostic markers in potato. Crop Sci 47:S106–S111
Geigenberger R, Reimholz R, Geiger M et al (1997) Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit. Planta 201:502–518
Geigenberger P, Reimholz R, Deiting U et al (1999) Decreased expression of sucrose phosphate synthase strongly inhibits the water stress-induced synthesis of sucrose in growing potato tubers. Plant J 19:119–129
Giraud E, Ho LHM, Clifton R et al (2008) The absence of alternative oxidase1a in Arabidopsis results in acute sensitivity to combined light and drought stress. Plant Physiol 147:595–610
Gosti F, Beaudoin N, Serizet C et al (1999) ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11:1897–1909
Grillo S, Costa A, Tucci M et al (1995) Regulation of gene expression during cellular adaptation to water stress. In: Grillo S, Leone A (eds) Proceedings of the workshop physical stresses in plants: genes and their products for tolerance. Maratea, Italy, pp 163–169
Gustin MC, Albertyn J, Alexander M et al (1998) MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev 62:1264–1300
Handa S, Bressan RA, Handa AK (1983) Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol 73:834–843
Harris PM (1978) The potato crop. Chapman and Hall, London
Haverkort AJ, van de Waart M, Bodlaender KBA (1990) The effect of early drought stress on numbers of tubers and stolons of potato in controlled and field conditions. Potato Res 33:89–96
Heuer B, Nadler A (1998) Physiological response of potato plants to soil salinity and water deficit. Plant Sci 137:43–51
Hu H, Dai M, Yao J et al (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. PNAS 103:12987–12992
Iwama K (2008) Physiology of the potato: new insights into root system and repercussions for crop management. Potato Res 51:333–353
Iyer S, Caplan A (1998) Products of proline catabolism can induce osmotically regulated genes in rice. Plant Physiol 116:203–211
Jefferies RA (1993) Cultivar responses to water stress in potato: effects of shoot and roots. New Phytol 123:491–498
Jefferies RA (1993) Responses of potato genotypes to drought. I. Expansion of individual leaves and osmotic adjustment. Ann Appl Biol 122:93–104
Jefferies RA, MacKerron DKL (1993) Responses of potato genotypes to drought. II. Leaf area index, growth and yield. Ann Appl Biol 122:105–112
Jiang GH, He YQ, Xu CG et al (2004) The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross. TAG 108:688–698
Juenger TE, Mckay JK, Hausmann N et al (2005) Identification and characterization of QTL underlying whole plant physiology in Arabidopsis thaliana: Δ13C, stomatal conductance and transpiration efficiency. Plant Cell Environ 28:697–708
Karlson D, Nakaminami K, Toyomasu T et al (2002) A cold-regulated nucleic acid-binding protein of winter wheat shares a domain with bacterial cold shock proteins. J Biol Chem 277:35248–35256
Kim JS, Park SJ, Kwak KJ et al (2007) Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote cold adaptation process in Escherichia coli. Nucleic Acids Res 35:506–516
Kloosterman B, De Koeyer D, Griffiths R et al (2008) Genes driving potato tuber initiation and growth: identification based on transcriptional changes using the POCI array. Funct Integr Genomics 8:329–340
Kolbe A, Tiessen A, Schluepmann H et al (2005) Trehalose 6-phosphate regulates starch synthesis via posttranslational redox activation of ADP-glucose pyrophosphorylase. PNAS 102:11118–11123
Kumar D, Minhas JS (1999) Effect of water stress on photosynthesis, productivity and water status in potato. J Indian Potato Assoc 26:7–10
Lahlou O, Ledent JF (2005) Root mass and depth, stolons and roots formed on stolons in four cultivars of potato under water stress. Europ J Agron 22:159–173
Langenkaemper G, Manac’h N, Broin M et al (2001) Accumulation of plastid lipid-associated proteins (fibrillin/CDSP34) upon oxidative stress, ageing and biotic stress in Solanaceae and in response to drought in other species. J Exp Bot 52:1545–1554
Levitt J (1980) Responses of plants to environmental stresses, vol. 2, Water, radiation, salt and other stresses. Academic, New York, pp 93–128
Levy D (1983) Varietal differences in the response of potatoes to repeated short periods of water stress in hot climates. 1. Turgor maintenance and stomatal behaviour. Potato Res 26:303–313
Levy D (1986) Genotypic variation in the response of potatoes (Solanum tuberosum L.) to high ambient temperatures and water deficit. Field Crops Res 15:85–96
Li J, Burmeister M (2005) Genetical genomics: combining genetics with gene expression analysis. Human Mol Gen 14:R163–R169
Liu F, Jensen CR, Shahanzari A et al (2005) ABA regulated stomatal control and photosynthetic water use efficiency of potato (Solanum tuberosum L.) during progressive soil drying. Plant Sci 168:831–836
van Loon CD (1981) Effect of water stress on potato growth, development, and yield. American Potato J 58:51–69
Lynch DR, Tai GCC (1989) Yield and yield component response of eight potato genotypes to water stress. Crop Sci 29:207–1211
Malosetti M, van der Linden CG, Vosman B et al (2006) A mixed-model approach to association mapping using pedigree information with an illustration of resistance to Phytophthora infestans in Potato. Genetics 175:879–889
Man AL, Purcell PC, Hannappel U et al (1997) Potato SNF1-related protein kinase: molecular cloning, expression analysis and peptide kinase activity measurements. Plant Mol Biol 34:31–43
Mane S, Vazquez Robinet C, Ulanov A et al (2008) Molecular and physiological adaptation to prolonged drought stress in the leaves of two Andean potato genotypes. Funct Plant Biol 35:669–688
Martin MW, Miller DE (1987) Alterations in irrigation schedules and rates to eliminate potato genotypes susceptible to water stress. In: Foldo NE, Hansen SE, Nielsen N et al (eds) Abstracts of conference papers and posters of the 10th triennial conference of the European Association for Potato Research. Aalborg, Denmark, p 21
Martin MW, Miller DE (1987) Advantages of water-stress resistant genotypes in the Northwest. American Potato J 64:448–449
Martin RJ, Jamieson PD, Wilson DR et al (1992) Effects of soil moisture deficits on yield and quality of ‘Russet Burbank’ potatoes. New Zealand J Crop Horticultural Sci 20:1–9
Masle J, Gilmore SR, Farquhar GD (2005) The ERECTA gene regulates plant transpiration efficiency in Arabidopsis. Nature 436:866–870
van der Mescht A, de Ronde JA, Merwe T et al (1998) Changes in free proline concentrations and polyamine levels in potato leaves during drought stress. South African J Sci 94:347–350
van der Mescht A, de Ronde JA, Slabbert MM et al (2007) Enhanced drought tolerance in transgenic potato expressing the Arabidopsis thaliana Cu/Zn superoxide dismutase gene. S Afr J Sci 103:169–173
Miernyk JA (2001) The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and diverse family of chaperones. Cell Stress Chaperones 6:209–218
Miller G, Shulaev V, Mittler R (2008) Reactive oxygen signaling and abiotic stress. Physiol Plantarum 133:481–489
Mould RD, Rutherfoord RJ (1980) The effect of moisture stress during consecutive growth stages on tuber yield and quality of BP1 potatoes (Solanum tuberosum L.). Crop Prod 9:89–92
Nelson DE, Repetti PP, Adams TR et al (2007) Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA 104:16450–16455
Nguyen TT, Klueva N, Chamareck V et al (2004) Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Gen Genom 272:35–46
Ozturk ZN, Talamé V, Deyholos M et al (2002) Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. Plant Mol Biol 48:551–573
Pajerowska KM, Parker JE, Gebhardt C (2005) Potato homologues of Arabidopsis thaliana genes functional in defense signaling—Identification, genetic mapping and molecular cloning. Mol Plant Microb Interact 18:1107–1119
Rensink WA, Iobst S, Hart A et al (2005) Gene expression profiling of potato responses to cold, heat, and salt stress. Funct Integr Genomics 5:201–207
Rizhsky L, Liang H, Mittler R (2002) The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiol 130:1143–1151
Rodrigues SM, Andrade MO, Soares-Gomes AP et al (2006) Arabidopsis and tobacco plants ectopically expressing the soybean antiquitin-like ALDH7 gene display enhanced tolerance to drought, salinity, and oxidative stress. J Exp Bot 57:1909–1918
Sakuma Y, Maruyama K, Osakabe Y et al (2006) Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18:1292–1309
Schafleitner R, Gaudin A, Gutierrez RO et al (2007) Proline accumulation and real time PCR expression analysis of genes encoding enzymes of proline metabolism in relation to drought tolerance in Andean potato. Acta Physiologae Plantarum 29:1861–1664
Schafleitner R, Gutierrez R, Espino R et al (2007) Field screening for variation of drought tolerance in Solanum tuberosum L. by agronomical, physiological and genetic analysis. Potato Res 50:71–85
Schafleitner R, Gutierrez RO, Gaudin A et al (2007) Capturing candidate drought tolerance traits in two native Andean potato clones by transcription profiling of field grown plants under water stress. Plant Physiol Biochem 45:673–690
Selote DS, Bharti S, Khanna-Chopra R (2004) Drought acclimation reduces O2* accumulation and lipid peroxidation in wheat seedlings. Biochem Biophys Res Commun 314:724–729
Serraj R, Sinclair TR (2002) Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant Cell Environ 25:333–341
Sheveleva E, Chmara W, Bohnert HJ et al (1997) Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L. Plant Physiol 115:1211–1219
Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217–223
Sobeih W, Dodd IC, Bacon MA et al (2004) Long-distance signals regulating stomatal conductance and leaf growth in tomato (Lycopersicon esculentum) plants subjected to partial root zone drying. J Exp Bot 55:2353–2364
Spitters CJT, Schapendonk AHCM (1990) Evaluation of breeding strategies for drought tolerance in potato by means of crop growth simulation. Plant Soil 123:193–203
Stiller I, Dulai S, Kondrák M et al (2008) Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae. Planta 227:299–308
Streeter JG, Lohnes DG, Fioritto RJ (2001) Pattern of pinitol accumulation in soybean plants and relationships to drought tolerance. Plant Cell Environ 24:429–438
Sun W, Bernard C, van de Cotte B et al (2001) At-HSP17.6A, encoding a small heat shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. Plant J 27:07–415
Taji T, Ohsumi C, Iuchi S et al (2002) Important roles of drought- and cold inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant J 29:417–426
Tang L, Kim MD, Yang K-S et al (2008) Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res 17:705–715
Tang L, Tang H, Kwak SS et al (2008) Improving potato plants oxidative stress and salt tolerance by gene transfer both of Cu/Zn superoxide dismutase and ascorbate peroxidase. China Biotech 28:25–31
Teulat B, This D, Khairallah M et al (1998) Several QTLs involved in osmotic adjustment trait variation in barley (Hordeum vulgare L). TAG 96:688–698
Tiwari BS, Belenghi B, Levine A (2002) Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. PNAS 128:1271–1281
Tourneux C, Devaux A, Camacho MR et al (2003) Effect of water shortage on six potato genotypes in the highlands of Bolivia (II): water relations, physiological parameters. Agronomie 23:181–190
Trebejo I, Midmore DJ (1990) Effect of water stress on potato growth, yield and water use in a hot and a cool tropical climate. J Agric Sci 114:321–334
Tripathy JN, Zhang J, Robin S et al (2000) QTLs for cell-membrane stability mapped in rice (Oryza sativa L.) under drought stress. TAG 100:1197–1202
Urao T, Yakubov B, Satoh R et al (1999) A transmembrane hybrid-type histidine-aspartate kinase in Arabidopsis functions as an osmosensor. Plant Cell 11:1743–1754
Vasquez-Robinet C, Mane SP, Ulanov AV et al (2008) Physiological and molecular adaptations to drought in Andean potato genotypes. J Exp Bot 59:2109–2123
Vos J, Groenwold J (1988) Mean annual yield reductions of potatoes due to water deficits for Dutch weather conditions. Acta Horticulturae 214:61–70
Warren CR (2007) Stand aside stomata, another actor deserves centre stage: the forgotten role of the internal conductance to CO2 transfer. J Exp Bot 59:1475–1487
Wasilewska A, Vlad F, Sirichandra C (2008) An update on abscisic acid signaling in plants and more. Mol Plant 1:198–217
Watkinson JI, Hendricks L, Sioson AA et al (2006) Accessions of Solanum tuberosum ssp. andigena show differences in photosynthetic recovery after drought stress as reflected in gene expression profiles. Plant Sci 171:745–758
Weisz R, Kaminski J, Smilowitz Z (1994) Water deficit effects on potato leaf growth and transpiration: utilizing fraction extractable soil water for comparison with other crops. Am Pot J 71:829–840
Wu G, Shao HB, Chu L-Y et al (2007) Insights into molecular mechanisms of mutual effect between plants and the environment. A review. Agron Sustain Dev 27:69–78
Wu SB, Wirthensohn MG, Hunt P et al (2008) High resolution melting analysis of almond SNPs derived from ESTs. TAG 118:1–14
Yamada Y, Yasui H, Sakurai H (2008) Suppressive effect of caffeic acid and its derivatives on the generation of UVA-induced reactive oxygen species in the skin of hairless mice and pharmacokinetic analysis on organ distribution of caffeic acid in ddY mice. Photochem Photobiol 82:1668–1676
Yancey PH (2005) Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses. J Exp Biol 208:2819–2830
Yang L, Tang R, Zhu J et al (2008) Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana. Plant Mol Biol 66:329–343
Zvia K, Bar-Zvi D (2008) Synergism between the chaperone-like activity of the stress regulated ASR1 protein and the osmolyte glycine-betaine. Planta 227:1213–1219
Acknowledgements
Many thanks to all collaborators and colleagues who contributed data and information for this review and to the two anonymous reviewers of this paper for their helpful comments. The work performed at CIP cited in this review has been supported by the governments of Austria and Luxembourg and by the Generation Challenge Program.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by: Paul Moore
Rights and permissions
About this article
Cite this article
Schafleitner, R. Growing More Potatoes with Less Water. Tropical Plant Biol. 2, 111–121 (2009). https://doi.org/10.1007/s12042-009-9033-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12042-009-9033-6