Abstract
Acinetobacter sp. SMIC-1, Cupriavidus sp. SMIC-2, Pseudomonas sp. SMIC-3, Paracoccus sp. SMIC-4, and Pseudomonas sp. SMIC-5 capable of mineralizing xenobiotics (manmade organic compounds) that are diethyleneglycol monomethyleher (DGMME), 1-amino-2-propanol (APOL), 1-methyl-2-pyrrolidinone (NMP), diethyleneglycol monoethylether (DGMEE), tetraethyleneglycol (TEG) and tetrahydrothiophene 1,1-dioxide (Sulfolane) were immobilized mixedly in polyethyleneglycol carrier (SMIC-PEG). TGGE technique was employed to analyze variation of the immobilized bacterial community during xenobiotics being mineralized. The SMIC-PEG mineralized more than 95% of the xenobiotics except sulfolane in 6 days. When activated sludge (AS) was co-immobilized with SMIC community in PEG carrier (AS-SMIC-PEG), degradation efficiency of DGMEE, NPM was a little decreased; however, the degradation of other xenobiotics was neither increased nor decreased significantly. The bacterial community diversity in the SMIC-PEG was gradually decreased in proportion to incubation time in a batch cultivation reactor. SMIC strains in AS-SMIC-PEG were substituted by other bacterial community after 6 days of incubation time in batch cultivation reactor. The SMIC-PEG mineralized around 90% of xenobiotics in a continuous pilot reactor when 100 or 200 mg/L of xenobiotics was fed for 8 hr of hydraulic retention time (HRT); however, the mineralization efficiency was decreased significantly to around 75% when 200 mg/L of xenobiotics was fed for 4 hr of HRT. The mineralization effect of AS-SMIC-PEG for xenobiotics was lower than SMIC-PEG. Bacterial community diversity in both SMIC-PEG and AS-SMIC-EG was decreased in proportion to operation time in the continuous pilot reactor; however, some of them were maintained during operation for more than 50 days.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
M. Alexander, Environ. Sci. Technol., 19, 106 (1985).
J. R. Van der Meer, W. De Vos, S. Harayama and A. J. B. Zehnder, Microbiol. Rev., 56, 677 (1992).
J. R. Van der Meer, W. Roelofsen, G. Schraa and A. J. B. Zehner, FEMS Microbiol. Ecol., 45, 333 (1987).
C.M. Aelion, D. C. Dobbins and F.K. Pfaender, Appl. Microbiol. Biotechnol., 8, 75 (1989).
J. T. Wilson, J. F. McNabb, J.W. Cochran, T.H. Wang, M. B. Tomson and P. B. Bedient, Environ. Toxicol. Chem., 4, 721 (1985).
J. C. Spain, P.H. Pritchard and A.W. Bourquin, Appl. Environ. Microbiol., 40, 726 (1980).
D. L. Lewis, H. P. Kollig and R. E. Hodson, Appl. Environ. Microbiol., 55, 598 (1986).
C. M. Swindoll, C. M. Aellon and F.K. Pfaender, Appl. Environ. Microbiol., 54, 212 (1988).
E. J. Bouwer and P. L. McCarty, Environ. Sci. Technol., 16, 836 (1982).
H.H. Tabak, S.A. Quave, C. I. Mashni and E. F. Barth, J. Water Pollut. Control Fed., 53, 1503 (1981).
S. Simkins and M. Alexander, Appl. Environ. Microbiol., 47, 1299 (1984).
R.V. Subba-Rao, H. E. Rubin and M. Alexander, Appl. Environ. Microbiol., 43, 1139 (1982).
M. Alexander, Science, 211, 132 (1981).
R. D. Vashon and B. S. Schwab, Environ. Sci. Technol., 16, 433 (1982).
J.R. Yordy and M. Alexander, Appl. Environ. Microbiol., 39, 559 (1980).
F.W. Hyde, G. R. Hunt and L. A. Errede, Appl. Environ. Microbiol., 57, 219 (1991).
J. P. Kutney, J.-D. Berset, G.M. Hewitt and M. Singh, Appl. Environ. Microbiol., 54, 1015 (1988).
J. P. Kutney, S. L. Choi, G. M. Hewitt, P. J. Salisbury and M. Singh, Appl. Environ. Microbiol., 49, 96 (1985).
X. Wang, Z. Gai, B. Yu, J. Feng, G. Xu, Y. Yuan, Z. Lin and P. Xu, Appl. Environ. Microbiol., 73, 6421 (2007).
T. Sumino, H. Nakamura, N. Mori and Y. Kawaguchi, J. Ferment. Technol., 73, 37 (1992).
S. J. Lee, Y.-W. Lee, J. Chung, J.K. Lee, J.Y. Lee, D. Jahng, Y. Cha and Y. Yu, Water Science & Technology, 57(8), 1191 (2008).
H.-S. Shin and D.-G. Jung, Bull. Korean Chem. Soc., 25, 806 (2004).
C.M. Aelion, C. M. Swindoll and F.K. Pfaender, Appl. Environ. Microbiol., 53, 2212 (1987).
T. Barkay and H. Pritchard, Microbiol. Sci., 5, 165 (1988).
S.R. Hutchins, M. B. Tomson, J. T. Wilson and C.H. Ward, Appl. Environ. Microbiol., 48, 1039 (1984).
E. L. Madsen, J. L. Sinclair and W. C. Ghiorse, Science, 252, 830 (1991).
J.C. Spain and P.A. van Veld, Appl. Environ. Microbiol., 45, 428 (1983).
P.M. Fedorak, J.D. Payzant, D. S. Montgomery and D.W. S. Westlake, Appl. Environ. Microbiol., 54, 1243 (1988).
P.M. Fedorak and D.W. S. Westlake, Can. J. Microbiol., 29, 497 (1983).
P.M. Fedorak and D.W. Westlake, Water Air Soil Pollut., 21, 225 (1984).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, J.K., Lee, W.J., Cho, YJ. et al. Variation of bacterial community immobilized in polyethylene glycol carrier during mineralization of xenobiotics analyzed by TGGE technique. Korean J. Chem. Eng. 27, 1816–1821 (2010). https://doi.org/10.1007/s11814-010-0291-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-010-0291-7