Abstract
The granulation process, physic-chemical properties, pollution removal ability and bacterial communities of aerobic granules with different feed-wastewater (synthetic wastewater, R1; swine wastewater, R2), and the change trend of some parameters of two types of granules in long-term operated reactors treating swine wastewater were investigated in this experiment. The result indicated that aerobic granulation with the synthetic wastewater had a faster rate compared with swine wastewater and that full granulation in R1 and R2 was reached on the 30th day and 39th day, respectively. However, although the feed wastewater also had an obvious effect on the biomass fraction and extracellular polymeric substances of the aerobic granules during the granulation process, these properties remained at a similar level after long-term operation. Moreover, a similar increasing trend could also be observed in terms of the nitrogen removal efficiencies of the aerobic granules in both reactors, and the average specific removal rates of the organics and ammonia nitrogen at the steady-state stage were 35.33 mg•g–1 VSS and 51.46 mg•g–1 VSS for R1, and 35.47 mg•g–1 VSS and 51.72 mg•g–1 VSS for R2, respectively. In addition, a shift in the bacterial diversity occurred in the granulation process, whereas bacterial communities in the aerobic granular reactor were not affected by the seed granules after long-term operation.
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References
Li P, Wang Y, Wang Y, Liu K, Tong L. Bacterial community structure and diversity during establishment of an anaerobic bioreactor to treat swine wastewater. Water Science and Technology, 2010, 61(1): 243–252
Morales N, Figueroa M, Fra-Vázquez A, Val del Río A, Campos J L, Mosquera-Corral A, Méndez R. Operation of an aerobic granular pilot scale SBR plant to treat swine slurry. Process Biochemistry, 2013, 48(8): 1216–1221
Figueroa M, Val del Rio A, Campos J L, Mosquera-Corral A, Mendez R. Treatment of high loaded swine slurry in an aerobic granular reactor. Water Science and Technology, 2011, 63(9): 1808
Yan L L, Liu Y, Ren Y, Wang X H, Liang H J, Zhang Y. The effect of pH on the efficiency of an SBR processing piggery wastewater. Biotechnology and Bioprocess Engineering, 2013, 18(6): 1230–1237
Gao D W, Liu L, Liang H, Wu W M. Aerobic granular sludge: characterization, mechanism of granulation and application to wastewater treatment. Critical Reviews in Biotechnology, 2011, 31(2): 137–152
Zhang X Y, Wang B B, Han Q Q, Zhao H M, Peng D C. Effects of shear force on formation and properties of anoxic granular sludge in SBR. Frontiers of Environmental Science & Engineering, 2013, 7 (6): 896–905
van Loosdrecht M C, Brdjanovic D. Anticipating the next century of wastewater treatment. Science, 2014, 344(6191): 1452–1453
Othman I, Anuar A N, Ujang Z, Rosman N H, Harun H, Chelliapan S. Livestock wastewater treatment using aerobic granular sludge. Bioresource Technology, 2013, 133(2): 630–634
Jungles M K, Figueroa M, Morales N, Val del Río Á,, da Costa R H R, Campos J L, Mosquera-Corral A, Méndez R. Start up of a pilot scale aerobic granular reactor for organic matter and nitrogen removal. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 2011, 86(5): 763–768
Fang H, Cai L, Yu Y, Zhang T. Metagenomic analysis reveals the prevalence of biodegradation genes for organic pollutants in activated sludge. Bioresource Technology, 2013, 129(2): 209–218
Yadav T C, Khardenavis A A, Kapley A. Shifts in microbial community in response to dissolved oxygen levels in activated sludge. Bioresource Technology, 2014, 165(8): 257–264
Liang B, Cheng H, Van Nostrand J D, Ma J, Yu H, Kong D, Liu W, Ren N, Wu L, Wang A, Lee D J, Zhou J. Microbial community structure and function of nitrobenzene reduction biocathode in response to carbon source switchover. Water Research, 2014, 54(4): 137–148
Sheng G, Li A, Li X, Yu H. Effects of seed sludge properties and selective biomass discharge on aerobic sludge granulation. Chemical Engineering Journal, 2010, 160(1): 108–114
Song Z, Pan Y, Zhang K, Ren N, Wang A. Effect of seed sludge on characteristics and microbial community of aerobic granular sludge. Journal of Environmental Sciences–China, 2010, 22(9): 1312–1318
Verawaty M, Pijuan M, Yuan Z, Bond P L. Determining the mechanisms for aerobic granulation from mixed seed of floccular and crushed granules in activated sludge wastewater treatment. Water Research, 2012, 46(3): 761–771
APHA. Standard Methods for the Examination for Water and Wastewater. 21th ed. Washington, D C: American Public Health Association, 1998
Adav S S, Lee D J. Extraction of extracellular polymeric substances from aerobic granule with compact interior structure. Journal of Hazardous Materials, 2008, 154(1–3): 1120–1126
Liu L, Gao D, Zhang M, Fu Y. Comparison of Ca2+ and Mg2+ enhancing aerobic granulation in SBR. Journal of Hazardous Materials, 2010, 181(1–3): 382–387
Kim B S, Kim B K, Lee J H, Kim M, Lim Y W, Chun J. Rapid phylogenetic dissection of prokaryotic community structure in tidal flat using pyrosequencing. Journal of Microbiology (Seoul, Korea), 2008, 46(4): 357–363
Huse S M, Dethlefsen L, Huber J A, Welch D M, Relman D A, Sogin M L. Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing. PLOS Genetics, 2008, 4(11): e1000255 doi.org/10.1371/annotation/3d8a6578-ce56-45aa-bc71- 05078355b851
Weber S D, Ludwig W, Schleifer K H, Fried J. Microbial composition and structure of aerobic granular sewage biofilms. Applied and Environmental Microbiology, 2007, 73(19): 6233–6240
Sheng G P, Yu H Q, Li X Y. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnology Advances, 2010, 28(6): 882–894
Wu L, Peng C, Peng Y, Li L, Wang S, Ma Y. Effect of wastewater COD/N ratio on aerobic nitrifying sludge granulation and microbial population shift. Journal of Environmental Sciences–China, 2012, 24(2): 234–241
Amorim C L, Maia A S, Mesquita R B, Rangel A O, van Loosdrecht M, TiritanME, Castro P M. Performance of aerobic granular sludge in a sequencing batch bioreactor exposed to ofloxacin, norfloxacin and ciprofloxacin. Water Research, 2014, 50(3): 101–113
Elifantz H, Horn G, Ayon M, Cohen Y, Minz D. Rhodobacteraceae are the key members of the microbial community of the initial biofilm formed in Eastern Mediterranean coastal seawater. FEMS Microbiology Ecology, 2013, 85(2): 348–357
Liu L, Gibson V, Huang X, Liu C X, Zhu G F. Effects of antibiotics on characteristics and microbial resistance of aerobic granules in sequencing batch reactors. Desalination andWater Treatment, 2015, (ahead-of-print): 1–10
Liu Y Q, Liu Y, Tay J H. The effects of extracellular polymeric substances on the formation and stability of biogranules. Applied Microbiology and Biotechnology, 2004, 65(2): 143–148
Liu L, Gao D W, Liang H. Effect of sludge discharge positions on steady-state aerobic granules in sequencing batch reactor (SBR). Water Science and Technology, 2012, 66(8): 1722–1727
Andreadakis A D. Anaerobic digestion of piggery wastes. Water Science and Technology, 1992, 25(1): 9–16
Anthonisen A C, Loehr R C, Prakasam T B, Srinath E G. Inhibition of nitrification by ammonia and nitrous acid. Journal- Water Pollution Control Federation, 1976, 48(5): 835–852
Verawaty M, Pijuan M, Yuan Z, Bond P L. Determining the mechanisms for aerobic granulation from mixed seed of floccular and crushed granules in activated sludge wastewater treatment. Water Research, 2012, 46(3): 761–771
Adav S S, Lee D J, Lai J Y. Microbial community of acetate utilizing denitrifiers in aerobic granules. Applied Microbiology and Biotechnology, 2010, 85(3): 753–762
Purkhold U, Pommerening-Röser A, Juretschko S, Schmid M C, Koops H P, Wagner M. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys. Applied and Environmental Microbiology, 2000, 66(12): 5368–5382
Daims H, Nielsen J L, Nielsen P H, Schleifer K H,Wagner M. In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants. Applied and Environmental Microbiology, 2001, 67(11): 5273–5284
Lv Y, Wan C, Lee D J, Liu X, Tay J H. Microbial communities of aerobic granules: granulation mechanisms. Bioresource Technology, 2014, 169(5): 344–351
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Liu, L., You, Q., Gibson, V. et al. Treatment of swine wastewater in aerobic granular reactors: comparison of different seed granules as factors. Front. Environ. Sci. Eng. 9, 1139–1148 (2015). https://doi.org/10.1007/s11783-015-0823-9
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DOI: https://doi.org/10.1007/s11783-015-0823-9