Abstract
Conjugated trienoic FA—α-eleostearic, β-eleostearic, and punicic acids—were preferentially isolated in pure form from the total methyl esters of the respective oils by simple silica gel TLC using hexane/diethyl ether as the mobile phase (94∶6). The subsequent saponification of the separated methyl ester fractions upon cooling at −20°C yielded individual acids in crystalline form. Melting points, conventional UV spectroscopy, and GLC confirmed the identity of the conjugated trienoic FA. The method finds potential application in benchscale reaction optimization using pure FA and in the preparation of standard reference materials of these acids, as these oils are widely used in the coatings industry.
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Devi, P.S. TLC as a tool for quantitative isolation of conjugated trienoic FA. J Amer Oil Chem Soc 80, 315–318 (2003). https://doi.org/10.1007/s11746-003-0696-y
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DOI: https://doi.org/10.1007/s11746-003-0696-y