Abstract
As a kind of enzyme widely existing in eukaryotic species, especially in grains and oil seeds, phytases play an important role in the degradation of some phosphates containing organic molecules. So far, phytases derived from various species have been successfully used as animal feed additives. It has also been experimentally verified that phytases have a potential use in generating crop germplasm with high phosphorus use efficiency, based on their biochemical role in releasing Pi from the phytate and its derivatives. In this paper, the biochemical properties, molecular characterizations, functions and the potential application perspective of phytases are reviewed and commented on, aiming at the further exploration of the biochemical and molecular characterizations, and promotion of the application of phytases, a kind of important enzyme possessing potential use in animal feeding and creation of high P use crop cultivars, in the future.
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Aaron A (2006). Expanding our knowledge of protein tyrosine phosphatase-like phytases: mechanism, substrate specificity and pathways of myo-inositol hexakisphosphate dephosphorylation. Dissertation for the Master’s Degree. Lethbridge, Alta.: University of Lethbridge, 10–13
Agostini J D, Ida E I (2006). Partially characterization and application of phytase extracted from germinated sun-flower seeds. Pesquisa Agropecuaria Brasileira, 41(6): 1041–1047
Andriotis V M E, Ross J D (2003). Isolation and characterisation of phytase from dormant Corylus avellana seeds. Phytochemistry, 64(3): 689–699
Angelis M, Gallo G, Corbo M R, McSweeney P L H, Faccia M, Giovine M, Gobbetti M (2003). Phytase activity in sourdough lactic acid bacteria: Purification and characterization of a phytase from Lactobacillus sanfranciscensis CB1. International Journal of Food Microbiology, 87: 259–270
Barrientos L, Scott J J, Murthy P P (1994). Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen. Plant Physiology, 106: 1489–1495
Berka R M, Rey M W, Brown K M, Byun T, Klotz A V (1998). Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus. Appllied and Environmental Microbiology, 64: 4423–4427
Bohn L, Josefsen L, Meyer A S, Rasmussen S K (2007). Quantitative analysis of phytate globoids isolated from wheat bran and characterization of their sequential dephosphorylation by wheat phytase. Journal of Agricultural and Food Chemistry, 55(18): 7547–7552
Brinch-Pedersen H, Olesen A, Rasmussen S K, Holm P B (2000). Generation of transgenic wheat (Triticum aestivum L.) for constitutive accumulation of an Aspergillus phytase. Molecular Breeding, 6: 195–206
Carla E H, Elizabeth A G (2001). A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings. Plant Physiology, 126: 1598–1608
Chen RM, Xue G X, Chen P, Yao B, Yang WZ, Ma Q L, Fan Y L, Zhao Z Y, Tarczynski M C, Shi J R (2008). Transgenic maize plants expressing a fungal phytase gene. Transgenic Research, 17(4): 633–643
Cho J S, Lee C W, Kang S H, Lee J C, Bok J D, Moon Y S, Lee H G, Kim S C, Choi Y J (2003). Purification and characterization of a phytase from Pseudomonas syringae MOK1. Current Microbiology, 47: 290–294
Chu H M, Guo R T, Lin T W, Chou C C, Shr H L, Lai H L, Tang T Y, Cheng K J, Selinger B L, Wang A H J (2004). Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure, 12: 2015–2024
Cosgrove D J (1970). Inositol phosphate phosphatase of microbiological origin. Inositol pentaphosphate intermediates in the dephosphorylation of the hexaphosphates of myo-inositol, scyllo-inositol, and D-chiro-inositol, by a bacterial (Pseudomonas sp.) phytase. Austrulia Journal of Biological Science, 23: 1207–1220
Craxton A, Caffrey J J, Burkhart W, Safrany S T, Shears S B (1997). Molecular cloning and expression of a rat hepatic multiple inositol polyphosphate phosphatase. Biochemistry Journal, 328: 75–81
Dassa E, Fsihi H, Marck C, Dion M, Kieffer-Bontemps M, Boquet P L (1992). A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA). Molecular and General Genetics, 229: 341–352
Denbow D M, Grabau E A, Lacy G H, Kornegay E T, Russell D R, Umbeck P F (1998). Soybeans transformed with a fungal phytase gene improve phosphorus availability for broilers. Poultry Science, 77(6): 878–881
Drakakaki G, Marcel S, Glahn R P, Lund E K, Pariagh S, Fisher R, Christou P, Stoger E (2005). Endosperm specific coexpression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Molecular Biology, 59: 869–880
Ehrlich K C, Montalbano B G, Mullaney E J, Dischinger H C, Ullah A J (1993). Identification and cloning of a second phytase gene (phyB) from Aspergillus niger. Biochemical and Biophysical Research Communicatons, 195: 53–57
Farhat A, Chouayekh H, Ben F M, Bouchaala K, Bejar S (2008). Gene cloning and characterization of a thermostable phytase from Bacillus subtilis US417 and assessment of its potential as a feed additive in comparison with a commercial enzyme. Molecular Biotechnology, 40(2): 127–135
Fu DW, Huang H Q, Meng K, Wang Y R, Luo H Y, Yang P L, Yuan T Z, Yao B (2009). Improvement of Yersinia frederiksenii phytase performance by a single amino acid substitution. Biotechnology and Bioengineering, 103(5): 857–864
George T S, Simpson R J, Hadobas P A, Richardson A E (2005). Expression of a fungal phytase gene in Nicotiana tabacum improves phosphorus nutrition of plants grown in amended soils. Plant Biotechnology Journal, 3(1): 129–140
Gibbins L N, Norris F W (1963). Phytase and acid phosphatase in the dwarf bean (Phaseolus vulgaris). Biochemistry Journal, 86: 67–71
Goel M, Sharma C B (1979). Multiple forms of phytase in germinating cotyledons of cucurbita-maxima. Phytochemistry, 18(12): 1939–1942
Gonnety J T, Niamke S, Meuwiah F B, N’guessan Kouadio E J, Kouame L P (2007). Purification, kinetic properties and physicochemical characterization of a novel acid phosphatase (AP) from germinating peanut (Arachis hypogaea) seed. Italian Journal of Biochemistry, 56(2): 149–157
Greiner R (2002). Purification and characterization of three phytate-degrading enzymes from germinated lupin seeds (Lupinus albus var. amiga), Journal of Agricultural and Food Chemistry, 50: 6858–6864
Greiner R (2004a). Degradation of myo-inositol hexakisphosphate by a phytate-degrading enzyme from Pantoea agglomerans. Protein Journal, 23: 577–585
Greiner R (2004b). Purification and properties of a phytate-degrading enzyme from Pantoea agglomerans. Protein Journal, 23: 567–576
Greiner R, Alminger M L (1999). Purification and characterization of a phytate-degrading enzyme from germinated oat (Avena sativa). Journal of the Science of Food and Agriculture, 79: 1453–1460
Greiner R, Alminger M L (2001). Stereospecificity of myo-inositol hexakisphosphate dephosphorylation by phytate-degrading enzymes of cereals. Journal of Food Biochemistry, 25: 229–248
Greiner R, Alminger M L, Carlsson N G (2001a). Stereospecificity of myo-inositol hexakisphosphate dephosphorylation by a phytate-degrading enzyme of baker’s yeast. Journal of Agricultural and Food Chemistry, 49: 2228–2233
Greiner R, Alminger M L, Carlsson N G, Muzquiz M, Burbano C, Cuadrado C, Pedrosa M M, Goyoaga C (2002a). Pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases from legume seeds. Journal of Agricultural and Food Chemistry, 50: 6865–6870
Greiner R, Carlsson N G, Alminger M L (2001b). Stereospecificity of myo-inositol hexakisphosphate dephosphorylation by a phytate-degrading enzyme of Escherichia coli. Journal of Biotechnology, 84: 53–62
Greiner R, Farouk A, Alminger M L, Carlsson N G (2002b). The pathway of dephosphorylation of myo-inositol hexakisphosphate by phytate-degrading enzymes of different Bacillus spp. Canada Journal of Microbiology, 48: 986–994
Greiner R, Haller E, Konietzny U, Jany K D (1997). Purification and characterization of a phytase from Klebsiella terrigena. Archives of Biochemistry and Biophysics, 341: 201–206
Greiner R, Jany K D, Alminger ML (2000). Identification and properties of myo-inositol hexakisphosphate phospho-hydrolases (phytases) from barley (Hordeum vulgare). Journal of Cereal Science, 3: 127–139
Greiner R, Konietzny U, Jany K D (1993). Purification and characterization of two phytases from Escherichia coli. Archives of Biochemistry and Biophysics, 303: 107–113
Greiner R, Konietzny U, Jany K D (1998). Purification and properties of a phytase from rye. Journal of Food Biochemistry, 22: 143–161
Guimaraes L H S, Terenzi H F, Jorge J A, Leone F D, Polizeli M T M (2004). Characterization and properties of acid phosphatases with phytase activity produced by Aspergillus caespitosus. Biotechnology and Appllied Biochemistry, 40: 201–207
Hamada A, Yamaguchi K, Harada M, Horiguchi K, Takahashi T, Honda H (2006). Recombinant, rice-produced yeast phytase shows the ability to hydrolyze phytate derived from seed-based feed, and extreme stability during ensilage treatment. Bioscience, Biotechnology, and Biochemistry, 70(6): 1524–1527
Hara A, Ebina S, Kondo A, Funaguma T (1985). A new type of phytase from pollen of Typha-Latifolia. Agricultural and Biological Chemistry, 49(12): 3539–3544
Hartingsveldt W, Zeijl C M J, Hartereld G M (1993). Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger. Gene, 127: 87–94
Hayakawa T, Suzuki K, Miura H, Ohno T, Igaue I (1990). Myoinositol polyphosphate intermediates in the dephosphorylation of phytic acid by acid-phosphatase with phytase activity from rice bran. Agricultural and Biological Chemistry, 54: 279–286
Hayakawa T, Toma Y, Igaue I (1989). Purification and characterization of acid-phosphatases with or without phytase activity from rice bran. Agricultural and Biological Chemistry, 53(6): 1475–1483
Hbel F, Beck E (1996). Maize root phytase. Plant Physiology, 112: 1429–1436
Hong C Y, Cheng K J, Tseng T H, Wang C S, Liu L F, Yu S M (2004). Production of two highly active bacterial phytases with broad pH optima in germinated transgenic rice seeds. Transgenic Research, 13(1): 29–39
Hong Y F, Liu C Y, Cheng K J, Hour A L, Chan M T, Tseng T H, Chen K Y, Shaw J F, Yu S M (2008). The sweet potato sporamin promoter confers high-level phytase expression and improves organic phosphorus acquisition and tuber yield of transgenic potato. Plant Molecular Biology, 67(4): 347–361
Huang H Q, Luo H Y, Wang Y R, Fu D W, Shao N, Wang G Z, Yang P L, Yao B (2008). A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions. Appllied Microbiology and Biotechnology, 80(3): 417–426
Huang H Q, Shao N, Wang Y R, Luo H Y, Yang P L, Zhou Z G, Zhan Z C, Yao B (2009). A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. Appllied Microbiology and Biotechnology, 83(2): 249–259
Jia Z, Golovan S, Ye Q (1998). Purification, crystallization and preliminary X-ray analysis of the Escherichia coli phytase. Acta Crystallographica, 54(4): 47–64
Jog S P, Garchow B G, Mehta B D, Murthy P P N (2005). Alkaline phytase from lily pollen: Investigation of biochemical properties. Archives of Biochemistry and Biophysics, 440: 133–140
Jongbloed AW, Kemme P A, Mroz Z (1996). Phytase in swine rations: impact on nutrition and environment. BASF Technical Symposium, 44–69
Kerovou J, Lauraeus M, Nurminen P (1998). Isolation, characterization, molecular gene cloning and sequencing of a novel phytase from Bacillus subtilis. Appllied and Environmental Microbiology, 64: 2079–2085
Kerovuo J, Lauraeus M, Nurminen P, Kalkkinen N, Apajalahti J (1998). Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis. Applied and Environmental Microbiology, 64: 2079–2085
Kerovuo J, Rouvinen J, Hatzack F (2000). Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus phytase: indication of a novel reaction mechanism. Journal of Biochemitry, 352: 623–628
Kim H W, Kim Y O, Lee J H, Kim K K, Kim Y J (2003). Isolation and characterization of a phytase with improved properties from Citrobacter braakii. Biotechnology Letters, 25: 1231–1234
Kim H, Eskin N A M (1987). Canola phytase isolation and characterization. Journal of Food Science, 52(5): 1353–1354
Kim T, Edward J, Mullaney, Jesus M, Porres, Karl R, Roneker, Sarah Crowe, Sarah Rice, Taegu Ko, Abul H J, Ullah, Catherine B, Daly, Ross W, Xin G L (2006). Shifting the pH profile of Aspergillus niger PhyA phytase to match the stomach pH enhances its effectiveness as an animal feed additive. Applied and Environmental Microbiology, 72(6): 4397–4403
Kim Y O, Kim H K, Bae K S, Yu J H, Oh T K (1998a). Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enzyme and Microbial Technology, 22: 2–7
Kim Y O, Lee J K, Kim H K, Yu J H, Oh T K (1998b). Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli. FEMS Microbiology Letters, 162(1): 185–191
Konietzny U, Greiner R (2002). Molecular and catalytic properties of phytate-degrading enzymes (phytases). International Journa of Food Science and Technology, 37: 791–812
Konietzny U, Greiner R, Jany K D (1994). Purification and characterization of a phytase from spelt. Journal of Food Biochemistry, 18(3): 165–183
Konietzny U, Greiner R, Jany K D (1995). Purification and characterization of a phytase from spelt. Journal of Food Biochemistry, 18: 165–183
Koonin E V (1994). Conserved sequence pattern in a wide variety of phosphoesterases. Protein Sci, 3: 356–358.
Kostrewa D, Grüninger-Leitch F, D’Arcy A, Broger C, Mitchell D, van Loon A P G M (1997). Crystal structure of phytase from Aspergillus ficuum at 2.5 A resolution. Nature Structural Biology, 4: 185–190
Laboure A M, Gagnon J, Lescure A M (1993). Purification and characterization of a phytase (myo-inositol-hexakisphosphate phosphohydrolase) accumulated in maize (Zea mays) seedlings during germination. Biochemistry Journal, 295: 413–419
Lei X G, Porres J M, Mullaney E J, Brinch-Pedersen H (2007). Phytase: Source, Structure and Application. In: Poilna J, MacCabe A P, eds. Industrial Enzymes, Structure, Function and Application. Berlin: Springer, 505–529
Li G L, Yang S H, Li M G, Qiao Y K, Wang J H (2009). Functional analysis of an Aspergillus ficuum phytase gene in Saccharomyces cerevisiae and its root-specific, secretory expression in transgenic soybean plants. Biotechnology Letters, 31(8): 1297–1303
Li J, Hegeman C E, Hanlon RW, Lacy G H, Denbow M D, Grabau E A (1997). Secretion of active recombinant phytase from soybean cell-suspension cultures. Plant Physiology, 114: 1103–1111
Li M, Osaki M, Honma M, Tadano T (1997). Purification and characterization of phytase induced in tomato roots under phosphorus-deficient conditions. Soil Science and Plant Nutrition, 43: 179–190
Lim D, Golovan S, Forsberg C W (2000). Crystal structures of Escherichia coli phytase and its complex with phytate. Nature Structural & Molecular Biology, 7: 108–113
Lim M H, Lee O H, Chin J E, Ko H M, Kim I C, Lee H B, Im S Y, Bai S (2008). Simultaneous degradation of phytic acid and starch by an industrial strain of Saccharomyces cerevisiae producing phytase and alpha-amylase. Biotechnology Letters, 30(12): 2125–2130
Lim P E, Tate M E (1973). The phytases: II. Properties of phytase fraction F1 and F2 from wheat bran and the myo-inositol phosphates produced by fraction F2. Biochimica et Biophysica Acta, 302: 326–328
Liu Q, Huang Q Q, Lei X G, Hao Q (2004). Crystallographic snapshots of Aspergillus fumigatus phytase, revealing its enzymatic dynamics. Structure, 12(9): 1575–1583
Loewus F A, Murthy P P N (2000). Myo-inositol metabolism in plants. Plant Science, 150: 1–19
Lolas G M, Markakis P (1977). Phytase of navy beans (Phaseolus vulgaris). Journal of Food Science, 42(4): 1094–1097
Mahajan A, Dua S (1997). Nonchemical approach for reducing antinutritional factors in rape seed (Brassica campestris var. toria) and characterization of enzyme phytase. Journal of Agricultural and Food Chemistry, 45(7): 2504–2508
Maiti I B, Majumber A L, Biswas B B (1974). Purification and mode of action of phytase from Phaseolus aureus. Phytochemistry, 13: 1047–1051
Mandal N C, Biswas B B, Burman S (1972). Metabolism of inositol phosphates. 3. Isolation, purification and characterization of phytase from germinating mung beans. Phytochemistry, 11(2): 495–502
Maugenest S, Martinez I, Godin B, Perez P, Lescure A M (1999). Structure of two maize phytase genes and their spatio-temporal expression during seedling development. Plant Molecular Biology, 39: 503–514
Maugenest S, Martinez I, Lescure A M (1997). Cloning and characterization of a cDNA encoding a maize seedling phytase. Biochemistry Journal, 322: 511–517
Mitchell D B, Vogel K, Weimann B, Pasamontes L, van Loon A P G M (1997). The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology, 143: 245–252
Mondal M K, Panda S, Biswas P (2007). Effect of microbial phytase in soybean meal based broiler diets containing low phosphorus. Poultry Science, 6: 201–206
Mullaney E J, Daly C B, Ullah A H J (2000). Advances in phytase research. Advances in Appllied Microbiology, 47: 157–199
Mullaney E J, Ullah A H J (1998). Conservation of the active site motif in Aspergillus niger (ficcum) pH 6.0 optimum acid phosphatase and kidney bean purple acid phosphatase. Biochemical and Biophysical Research Communications, 243: 471–473
Mullaney E J, Ullah A H J (2003). The term phytase comprises several different classes of enzymes. Biochemical and Biophysical Research Communications, 312: 179–184
Nagai Y, Funahashi S (1962). Phytase (myo-inositol-hexaphosphate phosphohydrolase) from wheat bran. purification and substrate specificity. Agricultural and Biological Chemistry, 26(12): 794–803
Nakano T, Joh T, Narita K, Hayakawa T (2000). The pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases from wheat bran of Triticum aestivum. Bioscience Biotechnology and Biochemistry, 64: 995–1003
Nakano T, Joh T, Tokumoto E, Hayakawa T (1999). Purification and characterization of phytase from bran of Triticum aestivum L. cv. Nourin. Food Science and Technology Research, 5: 18–23
O’Dell B, de Boland A R, Koirtyohann S R (1991). Distribution of phytate and nutritionally important elements among the morphological components of cereal grains. Journal of Agricultural and Food Chemistry, 20: 718–721
Ostanin K, Harms E H, Stevis P E, Kuciel R, Zhou M M, van Etten R L (1992). Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase. Journal of Biological Chemistry, 267: 22830–22836
Pasamontes L, Haiker M, Wyss M, Tessier M, van Loon A P G M (1997). Gene cloning, purification, and characterization of a heatstable phytase from the fungus Aspergillus fumingatus. Appllied and Environmental Microbiology, 63: 1696–1700
Pen J, Verwoerd T C, van Paridin P A, Beukeder R F, van der Elzen P J M, Geerse K (1993). Phytase-containing transgenic seed as a novel feed additive for improved phosphorus utilization. Bio/Technology, 11: 811–814
Phillippy B Q (1998). Purification and catalytic properties of a phytase from scallion (Allium fistulosum L.) leaves. Journal of Agricultural and Food Chemistry, 46: 3491–3496
Piddington C S, Houston C S, Paloheimo M, Cantrell M, Miettinen-Oinonen A, Nevalainen H, Rambosek J (1993). The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene, 133: 55–62
Ponstein A S, Bade J B, Verwoerd T C, Molendijk L, Storms J, Beudeker R F (2002). Stable expression of phytase (phyA) in canola (Brassica napus) seeds: towards a commercial product. Molecular Breeding, 10: 31–44
Powar V K, Jagannathan V (1982). Purification and properties of phytate-specific phosphatase from Bacillus subtilis. Journal of Bacteriology, 151(3): 1102–1108
Promdonkoy P, Tang K, Sornlake W, Harnpicharnchai P, Kobayashi R S, Ruanglek V, Upathanpreecha T, Vesaratchavest M, Eurwilaichitr L, Tanapongpipat S (2009). Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris. FEMS Microbiology Letters, 290(1): 18–24
Quan C S, Fan S D, Zhang L H, Tian W J, Ohta Y (2002). Purification and properties of a phytase from Candida krusei WZ-001. Journal of Bioscience and Bioengineering, 94: 419–425
Quan C S, Tian W J, Fan S D, Kikuchi J I (2004). Purification and properties of a low-molecular-weight phytase from Cladosporium sp. FP-1. Journal of Bioscience and Bioengineering, 97: 260–266
Ragon M, Hoh F, Aumelas A, Chiche L, Moulin G, Boze H (2009). Structure of Debaryomyces castellii CBS 2923 phytase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 65(4): 321–326
Rao D E, Rao K V, Reddy V D (2008). Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies. Journal of Appllied Microbiology, 105(4): 1128–1137
Ravindran V, Bryden W L, Kornegay E T (1995). Phytates: occurrence, bioavailability and implications in poultry nutrition. Poultry and Avain Biology Reviews, 6: 125–143
Reddy N R, Sathe S K, Salunkhe D K (1982). Phytates in legumes and cereals. Advances in Food Research, 28: 1–92
Reddy V A, Venu K, Rao D E, Rao K Y, Reddy V D (2009). Chimeric gene construct coding for bi-functional enzyme endowed with endoglucanase and phytase activities. Archives of Microbiology, 191(2): 171–175.
Richardson A E, Hadobas PA, Hayes J E (2001). Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. Plant Journal, 25(6): 641–649
Sajidan A, Farouk A, Greiner R, Jungblut P, Müller E C, Borriss R (2004). Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1. Appllied Microbiology and Biotechnology, 65: 110–118
Sariyska M V, Gargova S A, Koleva L A, Angelov A I (2005). Aspergillus niger phytase: purification and characterization. Biotechnology, 98–105
Segueilha L, Lambrechts C, Boze H, Moulin G, Galzy P (1992). Purification and properties of the phytase from Schwannio-myces castellii. Journal of Fermenttation and Bioengineering, 74: 7–11
Shao N, Huang H, Meng K (2008). Cloning, expression, and characterization of a new phytase from the phytopathogenic bacterium Pectobacterium wasabiae DSMZ 18074. Journal of Microbiology and Biotechnology, 18(7): 1221–1226
Shimizu M (1993). Purification and characterization of phytase and acid phosphatase produced by Aspergillus oryzae K1. Bioscience, Biotechnology and Biochemistry, 57: 1364–1365
Simons P C M, Versteegh A J, Jongbloed AW, Kemme P A, Slump P, Bos K D, Wolters G E, Buedeker R F, Verschoor G J (1990). Improvement of phosphorus availability by microbial phytase in broilers and pigs. Nutrition, 64: 525–540
Suzuki U, Yoshimura K, Takaishi M (1907). Ueber ein Enzyme phytase das Anhydrooxy-methylen disphosphorasure spaltet. Collections of Agricultu al Bulletins of Tokyo Imperial University, 7: 495–512
Tambe S M, Kaklij G S, Keklar S M, Parekh L J (1994). Two distinct molecular forms of phytase from Klebsiella aerogenes: Evidence for unusually small active enzyme peptide. Journal of Fermentation and Bioengineering, 77: 23–27
Tseng Y H, Fang T J, Tseng SM (2000). Isolation and characterization of a novel phytase from Penicillium simplicissimum. Folia Microbiologica. 45: 121–127
Tye A J, Siu F K Y, Leung T Y C, Lim B L (2002). Molecular cloning and the biochemical characterization of two novel phytases from Bacillus subtilis 168 and Bacillus licheniformis. Applied Microbiology and Biotechnology, 59: 190–197
Ullah A H J (1988). Aspergillus ficuum phytase: partial primary structure, substrate selectivity and kinetic characterization. Preparative Biochemistry, 18: 459–471
Ullah A H J, Cummins B J, Dischinger J H C (1991). Cyclohexanedione modification of arginine at the active site of Aspergillus ficuum phytase. Biochemical and Biophysical Research Communications, 178: 45–53
Ullah A H J, Dischinger Jr H C (1993). Aspergillus ficuum phytase: complete primary structure elucidation by chemical sequencing. Biochemical and Biophysical Research Communications, 192(2): 747–753
Ullah A H J, Gibson D M (1987). Extracellular phytase (EC.3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization. Preparative Biochemistry, 17: 63–91
Ullah A H J, Sethumadhavan K (2003). PhyA gene product of Aspergillus ficuum and Peniophora lycii produces dissimilar phytases. Biochemical and Biophysical Research Communications, 303: 463–468
Ullah A H J, Sethumadhavan K, Lei X G, Mullaney E J (2000). Biochemical characterization of cloned Aspergillus fumigatus phytase (phyA). Biochemical and Biophysical Research Communications, 275: 279–285
van Staden J, den Haan R, van Zyl W H, Botha A, Viljoen-Bloom M (2007). Phytase activity in Cryptococcus laurentii ABO 510. FEMS Yeast Research, 7(3): 442–448
vander Kaay J, van Haastert J M (1995). Stereospecificity of inositol hexaphosphate dephosphorylation by Paramecium phytase. Biochemistry Journal, 312: 907–910
Verwoerd T C, van Paridon PA, van Ooyen A J, van Lent JW, Hoekema A, Pen J (1995). Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves. Plant Physiology, 109(4): 1199–1205
Vohra A, Satyanarayana T (2002). Purification and characterization of a thermostable and acid-stable phytase from Pichia anomala. World Journal of Microbiology and Biotechnology, 18: 687–691
Wyss M, Brugger R, Kronenberger A, Remy R, Fimbell R, Oesterhelt G, Lehmann M, van Loon A P G M (1999). Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohyrolases): catalytic properties. Appllied and Environmental Microbiology, 65: 367–373
Xiao K, Harrison M J, Wang Z Y (2005). Transgenic expression of a novel M. truncatula phytase gene results in improved acquisition of organic phosphorus by Arabidopsis. Planta, 222: 27–36
Xiong A S, Yao Q H, Peng R H, Zhang Z, Xu F, Liu J G, Han P L, Chen J M (2006). High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris. Appllied Microbiology and Biotechnology, 72(5): 1039–1047
Yang W J, Matsuda Y, Sano S, Masutani H, Nakagawa H (1991). Purification and characterization of phytase from rat intestinal mucosa, Biochimica et Biophysica Acta, 1075: 75–82
Zhang W M, Edward J M, Xin G L (2007). Adopting selected hydrogen bonding and ionic interactions from Aspergillus fumigatus phytase structure improves the thermostability of Aspergillus niger PhyA phytase. Appllied and Environmental Microbiology, 73(9): 3069–3076
Zhang Z B, Kornegay E T, Radcliffe J S, Denbow DM, Veit H P, Larsen C T (2000). Comparison of genetically engineered Aspergillus and canola in weanling pig diets. Journal of Animal Science, 78: 2868–2878
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Li, R., Zhao, J., Sun, C. et al. Biochemical properties, molecular characterizations, functions, and application perspectives of phytases. Front. Agric. China 4, 195–209 (2010). https://doi.org/10.1007/s11703-010-0103-1
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DOI: https://doi.org/10.1007/s11703-010-0103-1