Abstract
Gl e 2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gl e 2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gl e 2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory, Gl e 2 was located between CIR362 and NAU2251b, NAU3860b, STV033, with a genetic distance 9.27 and 0.96 cM, respectively. This result is useful for cloning Gl e 2 gene by map-based cloning method.
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Cotton Research Institute, Chinese Academy of Agricultural Sciences. Genetics and Breeding of Cotton in China. Shandong: Shandong Science & Technology Press, 2003. 562
Bell A A, Stipanovic R D. The chemical composition biological activity and genetics of pigment glands in cotton. Proc Beltwide Cotton Prod Res Conf, 1977. 244–258
Afifi A, Bary A A, Kamel S A, et al. Bahtim 110, a new strain of Egyptian cotton free from gossypol. Emp Cot Grow Rew, 1966, 43: 112–120
Kohel R J, Lee J A. Genetic analysis of Egyptian glandless seeds cotton. Crop Sci, 1984, 24: 1119–1121
Tang C M, Min L F, Zhang T Z, et al. Genetic analysis for hai 1 strain of glandless cotton (G. barbadence L.) interaction between Gl e 2 and Gl 1. Cotton Sci Sin, 1996, 8: 138–140
Yuan Y L, Jing S R, Zhang T Z, et al. Studies on dominant glandless marker trait applied to heterosis in upland cotton. Seed, 1997, 6: 15–17
Lee J A. The genomic allocation of the principal foliar-gland loci in Gossypium hirsutum and Gossypium barbadense. Evolution, 1965, 19: 182–188
Yu J, Kohel R J, Dong J M, et al. Toward positional cloning of a major glandless gene in cotton. Proc Beltwide Cotton Conf, National Cotton Council, Memphis, TN, 2000. 516–517
Guo W Z, Cai C P, Wang C B, et al. A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics, 2007, 176: 527–541
Kohel R J, Lewis C F, Richmond T R. Texas marker-1: Description of a genetic standard for Gossypium hirsutum L. Crop Sci, 1970, 10: 670–671
Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol Biol Rep, 1993, 11: 122–127
Reddy O U K, Pepper A E, Saha S. New dinucleotide and trinucleotide microsatellite marker resources for cotton genome research. J Cotton Sci, 2001, 5: 103–113
Nguyen T B, Giband M, Brottier P. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet, 2004, 109: 167–175
Taliercio E, Allen R D, Essenberg M, et al. Analysis of ESTs from multiple Gossypium hirsutum tissues and identification of SSRs. Genome, 2006, 49: 306–319
Han Z G, Guo W Z, Song X L, et al. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton. Mol Gen Genomics, 2004, 272: 308–327
Han Z G, Wang C B, Song X L, et al. Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton. Theor Appl Genet, 2006, 112: 430–439
Zhang J, Wu Y T, Guo W Z, et al. Fast screening of microsatellite markers in cotton with PAGE/silver staining. Cotton Sci Sin, 2000, 12: 267–269
Li Z H, Luo P. SPSS for Windows. 2rd ed. Beijing: Publishing House of Electronics Industry, 2005. 87–89
van Ooijen J W, Voorrips R E. JoinMap Version 3.0: Software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen. 2001
Fryxell P A. A revision of Australian species of Gossypium with observation on occurrence of Thespesia in Australia (Malvaceae). Austr J Bot, 1965, 18: 71–102
Zhu S J, Jiang Y R, Reddy N, et al. Breeding, introgression and inheritance of delayed gland morphogenesis trait from Gosspium bickii into upland cotton germplasm. Chin Sci Bull, 2004, 49: 2470–2476
Chen X Y, Chen Y, Heinstein P, et al. Cloning, expression and characterization of (+)-δ-cadinene synthase: A catalyst for cotton phytoalexin biosynthesis. Arch Biochem Biophys, 1995, 324: 255–266
Liu C J, Heinstein P, Chen X Y. Expression pattern of genes encoding farnesyl diphosphate synthase and sesquiterpcne cyclase in cotton suspension cultured cells treated with fungal elicitors. Mol Plant-Microbe Inter, 1999, 12: 1095–1104
Meng Y L, Jia J W, Liu C J, et al. Coordinated accumulation of (+)-8-cadinene synthase mRNAs and gossypol in developing seeds of Gossypium hirsurum and a new member of the cad1 family from G. arboreum. J Nat Prod, 1999, 62: 248–252
Tan X P, Liang W Q, Liu C J, et al. Expression pattern of (+)-δ-cadinene synthase genes and biosyn thesis of sesquiterpcne aldehydes in plants of Gossypium arboreum L. Planta, 2000, 210: 644–651
Luo P, Wang Y H, Wang G D, et al. Molecular cloning and functional identification of (+)-δ-cadinene-8-hydroxylase, a cytochrome P450 momooxygenase (CYP70-6B1) of cotton sesquiterpene biosynthesis. Plant J, 2001, 28: 95–104
Xu Y H, Wang J W, Wang S, et al. Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-δ-cadinene synthase-A1. Plant Physiol, 2004, 135(1): 507–515
Chang P A, Li B, Ni X M, et al. Molecular cloning and expression analysis of a RanBP2 zinc finger protein gene in upland cotton (Gossypium hirsutum L.). Colloids Surf B Biointerfaces, 2007, 55: 153–158
Sunilkumar G, Campbell L M, Puckhaber L, et al. Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc Natl Acad Sci USA, 2006, 103: 18054–18059
Li W T, Zeng R Z, Zhang Z M, et al. Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.). Chin Sci Bull, 2006, 51: 675–680
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Supported by the National Basic Research Program of China (Grant No. 2007CB108805) and Program for Changjiang Scholars and Innovative Research Team in University (Grant No. IRT0432)
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Dong, C., Ding, Y., Guo, W. et al. Fine mapping of the dominant glandless Gene Gl e 2 in Sea-island cotton (Gossypium barbadense L.). Chin. Sci. Bull. 52, 3105–3109 (2007). https://doi.org/10.1007/s11434-007-0468-6
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DOI: https://doi.org/10.1007/s11434-007-0468-6