Abstract
The temperature gradient along the altitude transect of Mt. Jianfengling provides a good opportunity to establish and evaluate the microbial lipid-based environmental proxies. The soils collected from 14 different altitudes of Mt. Jianfengling contain abundant microbial fatty acids and fatty alcohols, including iso/anteiso fatty acids (i/aC12:0-i/aC19:0), 10-Me-C16:0 fatty acid, iso/anteiso fatty alcohols (i/aC13-iC26), 10-Me-C16:0 fatty alcohol and unsaturated fatty alcohols, which can indicate a strong microbial activity in the Jianfengling soils. The branched and unsaturated fatty alcohols can be only detected when saponification is performed, implying that these lipids are present as the constituents of bacterial wax esters in the soils. The ratio of aC15/iC15 fatty acids is positively correlated with altitude, suggesting that the decrease in temperature can induce the increase in the relative abundance of anteiso C15 fatty acid. In contrast, the ratio of aC15/iC15 fatty alcohols and of aC15/nC15 fatty alcohols both decrease with increased altitude or decreased temperature. Similarly, the ratio of nC18:1/nC18:0 fatty alcohols also decreases with decreased temperature, which is opposite to the previous observation that unsaturation of fatty acids in microorganism increases in response to decreased temperature. Besides, the average chain length (ACL) of long chain fatty alcohols (C22–C30) from leaf waxes and carbon preference index (CPI) of all n-fatty alcohols are also significantly correlated with altitude or mean annual temperature, demonstrating their potential for paleoclimate reconstruction. The correlation of microbial fatty acids and alcohols as well as ACL and CPI of plant wax-derived fatty alcohols with altitude may provide novel ways to reconstruct paleotemperature and paleoaltimetry.
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Annous B A, Becker L A, Bayles D O, et al. 1997. Critical role of anteiso-C15:0 fatty acid in the growth of Listeria monocytogenes at low temperatures. App Environ Microbiol, 63: 3887–3894
Costello E K, Schmidt S K. 2006. Microbial diversity in alpine tundra wet meadow soil: Novel Chloroflexi from a cold, water-saturated environment. Environ Microbiol, 8: 1471–1486
Dasgupta S, Fang J, Brake S S, et al. 2012. Biosynthesis of sterols and wax esters by Euglena of acid mine drainage biofilms: Implications for eukaryotic evolution and the early Earth. Chem Geol, 306-307: 139–145
De Boever E, Birgel D, Thiel V, et al. 2009. The formation of giant tubular concretions triggered by anaerobic oxidation of methane as revealed by archaeal molecular fossils (Lower Eocene, Varna, Bulgaria). Palaeogeog Palaeoclimat Palaeocl, 280: 23–36
Dodd R S, Poveda M M. 2003. Environmental gradients and population divergence contribute to variation in cuticular wax composition in Juniperus communis. Biochem Syst Ecol, 31: 1257–1270
Edgcomb M R, Sirimanne S, Wilkinson B J, et al. 2000. Electron paramagnetic resonance studies of the membrane fluidity of the foodborne pathogenic psychrotroph Listeria monocytogenes. BBA-Biomembranes, 1463: 31–42
Eglinton G, Hamilton R J. 1967. Leaf epicuticular waxes. Science, 156: 1322–1335
Ficken K J, Li B, Swain D L, et al. 2000. An n-alkane proxy for the sedimentary input of submerged/floating freshwater aquatic macrophytes. Org Geochem, 31: 745–749
Fierer N, Jackson R B. 2006. The diversity and biogeography of soil bacterial communities. Proc Natl Acad Sci USA, 103: 626–631
Frostegård A, Bååth E. 1996. The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fert Soils, 22: 59–65
Gao L, Nie J, Clemens S, et al. 2012. The importance of solar insolation on the temperature variations for the past 110 kyr on the Chinese Loess Plateau. Palaeogeogr Palaeoclimat Palaeoecol, 317-318: 128–133
Ghosh P, Garzione C N, Eiler J M. 2006. Rapid uplift of the Altiplano revealed through 13C–18O bonds in paleosol carbonates. Science, 311: 511–515
Hren M T, Pagani M, Erwin D M, et al. 2010. Biomarker reconstruction of the early Eocene paleotopography and paleoclimate of the northern Sierra Nevada. Geology, 38: 7–10
Hua NP, Kanekiyo A, Fujikura K, et al. 2007. Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol, 7: 1243–1249
Huang X, Meyers P A, Jia C, et al. 2013. Paleotemperature variability in central China during the last 13 ka recorded by a novel microbial lipid proxy in the Dajiuhu peat deposit. Holocene, doi: 10.1177/0959683613-483617
Ishige T, Tani A, Sakai Y, et al. 2003. Wax ester production by bacteria. Curr Opin Microbiol, 6: 244–250
Janssen P H. 2006. Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. App Environ Microbiol, 72: 1719–1728
Kaneda T. 1991. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev, 55: 288–302
Kim J H, Schouten S, Hopmans E C, et al. 2008. Global sediment core-top calibration of the TEX86 paleothermometer in the ocean. Geochim Cosmochim Acta, 72: 1154–1173
Lauber C L, Hamady M, Knight R, et al. 2009. Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. App Environ Microbiol, 75: 5111–5120
Li D, Zhao M, Tian J, et al. 2013. Comparison and implication of TEX86 and UK-37′ temperature records over the last 356 kyr of ODP Site 1147 from the northern South China Sea. Palaeogeogr Palaeoclimat Palaeoecol, 376: 213–223
Li J J, Huang J H, Xie S C. 2011. Plant wax and its response to environmental conditions: an overview (in Chinese). Acta Ecol Sin, 31: 565–574
Liu W, Wang H, Zhang C L, et al. Distribution of glycerol dialkyl glycerol tetraether lipids along an altitudinal transect on Mt. Xiangpi, NE Qinghai-Tibetan Plateau, China. Org Geochem, 2013, 57: 76–83
Liu Z H, Pagani M, Zinniker D, et al. 2009. Global cooling during the Eocene-Oligocene climate transition. Science, 323: 1187–1190
Mudge S M, Norris C E. 1997. Lipid biomarkers in the Conwy Estuary (North Wales, U.K.): A comparison between fatty alcohols and sterols. Mar Chem, 57: 61–84
Mudge S M, Belanger S E, Nielsen A M. 2008. Fatty Alcohols: Anthropogenic and Natural Occurrence in the Environment. Cambridge, Royal Soceity of Chemistry
Müller P J, Kirst G, Ruhland G, et al. 1998. Calibration of the alkenone paleotemperature index UK-37′ based on core-tops from the eastern South Atlantic and the global ocean (60°N–60°S). Geochim Cosmochim Acta, 62: 1757–1772
Pearson A, Ingalls A E. 2013. Assessing the use of archaeal lipids as marine environmental proxies. Annu Rev Earth Planet Sci, 41: 359–384
Peterse F, van der Meer M T J, Schouten S, et al. 2009. Assessment of soil n-alkane δD and branched tetraether membrane lipid distributions as tools for paleoelevation reconstruction. Biogeosciences, 6: 2799–2807
Peterse F, Prins M A, Beets C J, et al. 2011. Decoupled warming and monsoon precipitation in East Asia over the last deglaciation. Earth Planet Sci Lett, 301: 256–264
Peterse F, van der Meer J, Schouten S, et al. 2012. Revised calibration of the MBT-CBT paleotemperature proxy based on branched tetraether membrane lipids in surface soils. Geochim Cosmochim Acta, 96: 215–229
Prahl F G, Wakeham S G. 1987. Calibration of unsaturation patterns in long-chain ketone compositions for paleotemperature assessment. Nature, 330: 367–369
Rampen S W, Willmott V, Kim J H, et al. 2012. Long chain 1,13- and 1,15-diols as a potential proxy for palaeotemperature reconstruction. Geochim Cosmochim Acta, 84: 204–216
Schouten S, Hopmans E C, Schefuss E, et al. 2002. Distributional variations in marine crenarchaeotal membrane lipids: A new tool for reconstructing ancient sea water temperatures? Earth Planet Sci Lett, 204: 265–274
Schouten S, Klimiuk A M, van der Meer M T J, et al. 2009. Occurrence and carbon metabolism of green non sulfur-like bacteria in Californian and Nevada Hot Spring microbial mats as revealed by wax ester lipid analysis. Geomicrobiol J, 26: 179–188
Sinninghe Damsté J S, Ossebaar J, Schouten S, et al. 2008. Altitudinal shifts in the branched tetraether lipid distribution in soil from Mt. Kilimanjaro (Tanzania): Implications for the MBT/CBT continental palaeothermometer. Org Geochem, 39: 1072–1076
Sun Q, Chu G, Liu G, et al. 2010. The occurrence and distribution of long chain alkenones in lakes (in Chinese). Acta Geosci Sin, 31: 485–494
Sun Q, Chu G, Liu M, et al. 2011. Distributions and temperature dependence of branched glycerol dialkyl glycerol tetraethers in recent lacustrine sediments from China and Nepal. J Geophys Res, 116(G1): G01008
Suutari M, Laakso S. 1992. Unsaturated and branched-chain fatty-acids in temperature adaptation of Bacillus subtilis and Bacillus megaterium. Biochim Biophys Acta, 1126: 119–124
Suutari M, Laakso S. 1994. Microbial fatty acids and thermal adaptation. Crit Rev Microbiol, 20: 285–328
Thiel V, Peckmann J, Seifert R, et al. 1999. Highly isotopically depleted isoprenoids: Molecular markers for ancient methane venting. Geochim Cosmochim Acta, 63: 3959–3966
Tierney J E, Russell J M, Eggermont H, et al. 2010. Environmental controls on branched tetraether lipid distributions in tropical East African lake sediments. Geochim Cosmochim Acta, 74: 4902–4918
Treignier C, Derenne S, Saliot A. 2006. Terrestrial and marine n-alcohol inputs and degradation processes relating to a sudden turbidity current in the Zaire canyon. Org Geochem, 37: 1170–1184
van der Meer M T J, Klatt C G, Wood J, et al. 2010. Cultivation and genomic, nutritional and lipid biomarker characterization of Roseiflexus strains closely related to predominant in situ populations inhabiting Yellowstone hot spring microbial mats. J Bacteriol, 192: 3033–3042
Volkman J K, Barrett S M, Blackburn S I. 1999. Eustigmatophyte microalgae are potential sources of C29 sterols, C22–C28 n-alcohols and C28-C32 n-alkyl diols in freshwater environments. Org Geochem, 30: 307–318
Wada M, Fukunaga N, Sasaki S. 1987. Effect of growth temperature on phospholipid and fatty-acid compositions in a psychrotrophic bacterium, Pseudomonas sp. Strain E-3. Plant Cell Physiol, 28: 1209–1217
Waltermann M, Hinz A, Robenek H, et al. 2005. Mechanism of lipid-body formation in prokaryotes: how bacteria fatten up. Mol Microbiol, 55: 750–763
Willecke K, Pardee A B. 1971. Fatty acid-requiring mutant of Bacillus subtilis defective in branched chain a-keto acid dehydrogenase. J Biol Chem, 246: 5264–5272
Weijers J W H, Schouten S, van den Donker J C, et al. 2007. Environmental controls on bacterial tetraether membrane lipid distribution in soils. Geochim Cosmochim Acta, 71: 703–713
Xie S C, Chen F H, Wang Z Y, et al. 2003. Lipid distributions in loess-paleosol sequences from northwest China. Org Geochem, 34: 1071–1079
Xie S, Huang X Y, Yang H, et al. 2013. An overview on microbial proxies for the reconstruction of past global environmental change (in Chinese). Quat Sci, 33: 1–18
Xiong Y Q, Wu F C, Wang T S, et al. 2009. Distribution characteristics of glycerol dialkyl glycerol tetraether lipids in Lake Dianchi Sediments (in Chinese). Acta Sediment Sin, 27: 1191–1198
Yang H, Ma X, Li Q, et al. 2009. Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea. Front Earth Sci, 3: 91–99
Yang H, Ding W, He G, et al. 2010. Archaeal and bacterial tetraether membrane lipids in soils of varied altitudes in Mt. Jianfengling in South China. J Earth Sci, 21: 277–280
Zelles L. 1999. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: A review. Biol Fert Soils, 29: 111–129
Zeng Q B, Ding M H. 1985. The relationship between the vertical distribution of tropical vegetation types and the water-heat regime of the Jiangfengling Mountain, Hainan island (in Chinese). Acta Phytoecol Geobotan Sin, 9: 297–305
Zhang Z, Zhao M, Eglinton G, et al. 2006. Leaf wax lipids as paleovegetational and paleoenvironmental proxies for the Chinese Loess Plateau over the last 170 kyr. Quat Sci Rev, 25: 575–594
Zhou H, Hu J, Ming L, et al. 2011. Branched glycerol dialkyl glycerol tetraethers and paleoenvironmental reconstruction in Zoigê peat sediments during the last 150 years. Chin Sci Bull, 56: 2456–2463
Zhou Z, Li Y D, Lin M X, et al. 2009. Change characteristics of thermal factors in tropical mountainrainforest area of Jianfengling, Hainan Island in 1980–2005 (in Chinese). Chin J Ecol, 28: 1006–1012
Zhu K, Bayles D O, Xiong A M, et al. 2005. Precursor and temperature modulation of fatty acid composition and growth of Listeria monocytogenes cold-sensitive mutants with transposon-interrupted branchedchain alpha-keto acid dehydrogenase. Microbiology-SGM, 151: 615–623
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Yang, H., Ding, W. & Xie, S. Distribution of microbial fatty acids and fatty alcohols in soils from an altitude transect of Mt. Jianfengling in Hainan, China: Implication for paleoaltimetry and paleotemperature reconstruction. Sci. China Earth Sci. 57, 999–1012 (2014). https://doi.org/10.1007/s11430-013-4729-8
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DOI: https://doi.org/10.1007/s11430-013-4729-8