Abstract
This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles’ or algae’s volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment.
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Jesús-Pérez NM, Lapizco-Encinas BH. Dielectrophoretic monitoring of microorganisms in environmental applications. Electrophoresis, 2011, 32: 2331–2357
Klodzinska E, Buszewski B. Electrokinetic detection and characterization of intact microorganisms. Anal Chem, 2009, 81: 8–15
Liu BF, Xu B, Zhang GS, Du W, Luo QM. Micro-separation toward systems biology. J Chromatogr A, 2006, 1106:19–28
Koubova V, Brynda E, Karasova L, Skvor J, Homola J, Dostalek J, Tobiska P, Rosicky J. Detection of foodborne pathogens using surface plasmon resonance biosensors. Sens Actuator B-Chem, 2001, 74: 100–105
Naravaneni R, Kaiser J. Rapid detection of food-borne pathogens by using molecular techniques. J Med Microbiol, 2005, 54: 51–54
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM. Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem, 1998, 70: 4974–4984
Whitesides GM. The origins and the future of microfluidics. Nature, 2006, 442: 368–373
Gascoyne PRC, Vykoukal J. Particle separation by dielectrophoresis. Electrophoresis, 2002, 23: 1973–1983
Hughes MP. Strategies for dielectrophoretic separation in laboratory-on-a-chip systems. Electrophoresis, 23: 2569–2582
Pohl HA. The motion and precipitation of suspensoids in divergent electric fields. J Appl Phys, 1951, 22: 869–871
Pohl HA, Hawk I. Separation of living and dead cells by dielectrophoresis. Science, 1966, 152: 647–649
Morgan H, Hughes MP, Green NG. Separation of submicron bioparticles by dielectrophoresis. Biophys J, 1999, 77: 516–525
Hughes MP, Morgan H, Rixon FJ. Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids. Eur Biophys J Biophys Lett, 2001, 30: 268–272
Gadish N, Voldman J. Emergent behavior in particle-laden microfluidic systems informs strategies for improving cell and particle separations. Anal Chem, 2006, 78: 7870–7876
Li H, Bashir R. Dielectrophoretic separation and manipulation of live and heat-treat cells of Listeria on microfabricated devices with interdigitated electrodes. Sens Actuator B-Chem, 2002, 86: 215–221
Markx GH, Dyda PA, Pethig R. Dielectrophoretic separation of bacteria using a conductivity gradient. J Biotechnol, 1996, 51: 175–180
Lapizco Encinas BH, Simmons BA, Cummings EB, Fintschenko Y. Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water. Anal Chem, 2004, 76: 1571–1579
Kang YJ, Li DQ, Kalams SA, Eid JE. DC-Dielectrophoretic separation of biological cells by size. Biomed Microdevices, 2008, 10: 243–249
Suehiro J, Zhou GB, Imamura M, Hara M. Dielectrophoretic filter for separation and recovery of biological cells in water. IEEE Trans Ind Appl, 2003, 39: 1514–1521
Kang Y, Cetin B, Wu Z, Li D. Continuous particle separation with localized AC-dielectrophoresis using embedded electrodes and an insulating hurdle. Electrochimica Acta, 2009, 54: 1715–1720
Gallo-Villanueva RC, Jesús-Pérez NM, Martínez-López, JI, Pacheco A, Lapizco-Encinas BH. Assessment of microalgae viability employing insulator-based dielectrophoresis. Microfluid Nanofluid, 2011, 10: 1305–1315
Srivastava SK, Gencoglu A, Minerick AR. DC insulator dielectrophoretic applications in microdevice technology: A review. Anal Bioanal Chem, 2011, 399: 301–321
Pohl HA. Dielectrophoresis. Cambridge: Cambridge University Press, 1978
Jones TB. Electromechanics of Particles. Cambridge: Cambridge University Press, 1995
Minerick AR. DC dielectrophoresis in lab-on-a-chip devices. In: Li D ed. Encyclopedia of Micro- and Nanofluidics. Berlin: Springer, 2008
Keshavamurthy SS, Leonard KM, Burgess SC, Minerick AR. Direct current dielectrophoretic characterization of erythrocytes: Positive ABO blood types. In: NSTI-Nanotech, Boston, 2008, 401–404
Ermolina I, Morgan H. The electrokinetic properties of latex particles: Comparison of electrophoresis and dielectrophoresis. J Colloid Interface Sci, 2005, 285: 419–428
Ozuna-Chacón S, Lapizco-Encinas BH, Rito-Palomares M, Martínez-Chapa SO, Reyes-Betanzo C. Performance characterization of an insulator-based dielectrophoretic microdevice. Electrophoresis, 2008, 29: 3115–3122
Lapizco-Encinas BH, Rito-Palomares M. Dielectrophoresis for the manipulation of nanobioparticles. Electrophoresis, 2007, 28: 4521–4538
Barrett LM, Skulan AJ, Singh AK, Cummings EB, Fiechtner GJ. Dielectrophoretic manipulation of particles and cells using insulating ridges in faceted prism microchannels. Anal Chem, 2005, 77: 6798–6804
Markx GH, Huang Y, Zhou XF, Pethig R. Dielectrophoretic characterization and separation of micro-organisms. Mkmbiology, 1994, 140: 585–591
Xuan X, Xu Bo, Sinton D, Li D. Electroosmosis flow with joule heating effects. Lab Chip, 2004, 4: 230–236
Erickson D, Sinton D, Li D. Joule heating and heat transfer in poly(dimethylsiloxane) microfluidic systems. Lab Chip, 2003 3: 141–149
Xia YN, Whitesides GM. Soft lithography. Annu Rev Mater Sci, 1998, 28: 153–184
Song YX, Zhang HP, Chon CH, Chen S, Pan XX, Li DQ. Counting bacteria on a microfluidic chip. Anal Chim Acta, 2010, 681: 82–86
Song YX, Zhang HP, Chon CH, Pan XX, Li DQ. Nanoparticle detection by microfluidic resistive pulse sensor with a submicron sensing gate and dual detecting channels-two stage differential amplifier. Sens Actuators B, 2011, 155: 930–936
Gregg EC, Steidley KD. Electrical counting and sizing of mammalian cells in suspension: an experimental evaluation. Biophys J, 1965, 5: 393–405
Carstensen EL, JR Cox HA, Mercer WB, Natale LA. Passive electrical properties of microorganisms I. conductivity of Escherichia coli AND Micrococcus lysodeikticus. Biophys J, 1965, 5: 289–300
Yang J, Huang Y, Wang XB, Becker FF, Gascoyne PR. Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation. Anal Chem, 1999, 71: 911–918
Ginzbuy M. Dunaliella:a green alga adapted to salt. Adv Botanical Res, 1987, 14: 93–99
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Song, Y., Yang, J., Shi, X. et al. DC dielectrophoresis separation of marine algae and particles in a microfluidic chip. Sci. China Chem. 55, 524–530 (2012). https://doi.org/10.1007/s11426-012-4533-x
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DOI: https://doi.org/10.1007/s11426-012-4533-x