Abstract
To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by 60Co gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brunner H. Radiation induced mutations for plant selection. Appl Radiat Isotop, 1995, 46(6): 589–594
Yang J H. Surface modification technology and application of high flux pulsed metal ion beam. Surf Technol (in Chinese), 2003, 32(1): 59–61
Fielden E M. The study of fast processes and transient species by electron pulse radiolysis. In: Proceedings of the NATO Advanced Study Institute Held in 1981. Italy: Reidel Dorgrecht Publishing Company, 1982. 49–52
Jia X J, Liu S L, Zhang L, Mi G D, Zhang Y L, Chen L H. Screening of rapamycin producing strain by high energy electrons mutation. J Hebei Acad Sci (in Chinese), 2005, 22(4): 27–29
Rui J Y, Jin J L, Ma X S, Huang P Z, Ma J H. Studies on the mutagenic effect of the electron beam in barley (hordeum vulgarel) I. The effects on M1 and M2 generations. J Radiat Res Radiat Proc (in Chinese), 1995, 13(1): 33–37
Hoglund E, Blomquist E, Carlsson J, Stenerlow B. DNA damage induced by radiation of different linear energy transfer: initial fragmentation. Int J Radiat Biol, 2000, 76(4): 539–547
Duke R C, Cohen J J. Morphological and biological assays of apoptosis. Curr Protoc Immunol, 1992, (1): 3–17
Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2001. 30–36
Friedland W, Dingfelder M, Jacob P, Paretzke H G. Calculated DNA double-strand break and fragmentation yields after radiation with He ions. Radiat Phys Chem, 2005, 72: 279–286
Bai Y S. Statistical methods for biological dose assessment. In: Wang J X, eds. Radiobiological Dosimeter (in Chinese). Beijing: Atomic Energy Press, 1997. 216–235
Jha K N, Bolton G L, Freeman G R. Temperature shifts in the optical spectra of solvated electrons in methanol and ethanol. J Phys Chem, 1972, 76(25): 3876–3883
Stankus A A, Xapsos M A, Kolanko C J, Gerstenberg H M, Blakely W F. Energy deposition events produced by fission neutrons in aqueous solutions of plasmid DNA. Int J Radiat Biol, 1995, 68(1): 1–9
Spotheim-Maurizot M, Charlier M, Sabattier R. DNA radiolysis by fast neutrons. Int J Radiat Biol, 1990, 57(2): 301–313
Wang J, Guo W S. On laser raised enzyme activity, Chin J Laser (in Chinese). 1997, 24(8): 765–768
Shao C L, Masahiro S, Yu Z L. A method of raising the detecting sensitivity to plasmid DNA of gel electrophoresis. Acta Laser Biol Sinica, 1999, 8(3): 238–242
Klimczak U, Ludwig D C, Mark F, Rettberg P, Schulte-Frohlinde D. Irradiation of plasmid and phage DNA in water alcohol mixtures: strand breaks and lethal damage as a function of scavenger concentration. Int J Radiat Biol, 1993, 64(5): 497–510
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the National Natural Science Foundation of China (Grant Nos. 30570376 and 50673078), the Shanghai Key Fundamental Project (Grant No. 06JC14068) and the Innovation Program of Shanghai Municipal Education Commission (Grant No. 08ZZ21)
Rights and permissions
About this article
Cite this article
Zhu, H., Xu, J., Li, S. et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules. Sci. China Ser. B-Chem. 51, 86–91 (2008). https://doi.org/10.1007/s11426-008-0017-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11426-008-0017-4