Abstract
X-ray photoelectron spectroscopy (XPS) has been employed in order to study the effects of proton irradiation on herring sperm single-stranded DNA. Systematic changes of the chemical shifts in the C, N, O, and P XPS line components as functions of the irradiation dose were observed, indicative of the bonding configurations in the DNA system. While the C 1s XPS lines showed weak blueshifts, the N 1s, O 1s, and P 2p XPS lines showed blueshifts with a marked dependence on the irradiation dose in a prominent manner. Our results show that linear energy transfer by charged particles and photons may have distinct molecular-level effects as the C 1s, N 1s, O 1s, and P 2p XPS lines showed redshifts in our previous study of effects of the γ-ray irradiation on the same system.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
T. B. Borak et al., Radiat. Res. 162, 687 (2004).
G. Gialanella, G. F. Grossi, M. F. Macchiato, M. Napolitano and P. R. Speranza, Radiat. Res. 96, 462 (1983).
D. Frankenberg, H. J. Brede, U. J. Schrewe, C. Steinmetz, M. Frankenberg-Schwager, G. Kasten and E. Pralle, Adv. Space Res. 25, 2085 (2000).
M. Hada and B. M. Sutherland, Radiat. Res. 165, 223 (2006).
P. Kundrat and R. D. Stewart, Radiat. Prot. Dosimetry 122, 169 (2006).
A. Campa et al., Int. J. Radiat. Biol. 81, 841 (2005).
W. Friedland, P. Jacob, P. Bernhardt, H. G. Paretzke and M. Dingfelder, Radiat. Res. 159, 401 (2003).
D. T. Goodhead, Int. J. Radiat. Biol. 65, 7 (1994).
J. F. Ward, Int. J. Radiat. Biol. 66, 427 (1994).
C. Di Pietro et al., Apoptosis 11, 57 (2006).
F. Ianzini, R. Cherubini and M. A. Mackey, Int. J. Radiat. Biol. 75, 717 (1999).
A. Antoccia, A. Sgura, M. Cavinato, R. Cherubini and C. Tanzarella, Radiat. Prot. Dosimetry 99, 197 (2002).
M. Belli et al., Adv. Space Res. 27, 393 (2001).
M. Belli, R. Cherubini, M. Dalla Vecchia, V. Dini, G. Esposito, G. Moschini, O. Sapora, G. Simone and M. A. Tabocchini, Int. J. Radiat. Biol. 78, 475 (2002).
E. J. Hall and A. Giaccia, Radiobiology for the Radiologist, 7th ed. (Lippcott Williams & Wilkins, Philadelphia, PA, 2011).
F. Yatagai, Biol. Sci. Space 18, 224 (2004).
K. B. Lee, J.-S. Lee, J.-W. Park, T.-L. Huh and Y. M. Lee, Exp. Mol. Med. 40, 118 (2008).
R. A. Rosenberg, J. M. Symonds, K. Vijayalakshmi, D. Mishra, T. M. Orlando and R. Naaman, Phys. Chem. Chem. Phys. 16, 15319 (2014).
C. A. Schroeder, E. Pluharova, R. Seidel, W. P. Schroeder, M. Faubel, P. Slavicek, B. Winter, P. Jungwirth and S. E. Bradforth, J. Am. Chem. Soc. 137, 201 (2015).
E. Lee, W. Hong, J. H. Han, D. M. Choi and C. E. Lee, J. Korean Phys. Soc. 67, 400 (2015).
E. Lee, J. H. Han, D. Kim, G. W. Jeon, C. E. Lee, Y. J. Park, H. D. Kim and J. Kim, J. Appl. Phys. 117, 014701 (2015).
W. Hong, E. Lee, J. K. Park and C. E. Lee, J. Appl. Phys. 113, 216101 (2013).
A. Ulman, An introduction to ultrathin organic films: From Langmuir-Blodgett films of self assemblies (Academic Press, New York, 1991).
D. Y. Petrovykh, H. Kimura-Suda, M. J. Tarlov and L. J. Whitman, Langmuir 20, 429 (2004).
U. Gelius, P. F. Heden, J. Hedman, B. J. Lindberg, R. Manne, R. Nordberg, C. Nordling and K. Siegbahn, Phys. Scr. 2, 70 (1970).
Ch. Y. Lee, P. Gong, G. M. Harbers, S. W. Grainger, D. G. Castner and L. J. Gamble, Anal. Chem. 78, 3316 (2006).
H. A. Noh and H. Cho, J. Rad. Prot. 36, 190 (2011).
C. J. May, H. E. Canavan and D. Castner, Anal. Chem. 76, 1114 (2004).
R. Rios-Font, J. Bertran, L. Rodriguez-Santiego and M. Sodupe, J. Phys. Chem. B 110, 5767 (2006).
F. Martin, P. D. Burrow, Z. Cai, P. Cloutier, D. Hunting and L. Sanche, Phys. Rev. Lett. 93, 068101 (2004).
K. K. Sharma, S. Purkayastha and W. A. Bernhard, Radiot. Res. 167, 501 (2007).
K. Fujii, K. Akamatsu and A. Yokoya, Int. J. Radiot. Biol. 80, 909 (2004).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, E., Lee, C.E. & Han, J.H. Effects of proton irradiation on single-stranded DNA studied by using X-ray photoelectron spectroscopy. Journal of the Korean Physical Society 69, 578–583 (2016). https://doi.org/10.3938/jkps.69.578
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3938/jkps.69.578