Abstract
The association between urinary cadmium and diabetes risk remains controversial. PubMed, Web of Science, China National Knowledge Infrastructure, and Wanfang Data updated on 21 June 2016 were searched for eligible publications. Pooled odds ratio (OR) with 95% confidence interval (CI) of diabetes for highest versus lowest level of urinary cadmium was calculated by using fixed-effect model or random-effect model. Dose-response relationship between urinary cadmium and diabetes was estimated by restricted cubic spline. A total of nine studies with 28,691 participants were included in this meta-analysis. The pooled OR of diabetes for the highest versus lowest level of urinary cadmium was 1.02 (95% CI, 1.00, 1.05; I 2 = 42.3%). In subgroup analysis, the ORs were 1.02 (95% CI 1.00, 1.05; I 2 = 0.9%) for studies conducted in Asia and 1.11 (95% CI 0.88, 1.41; I 2 = 86.3%) in America. For dose-response analysis, a linear relationship was found between urinary cadmium and the risk of diabetes (P for nonlinear = 0.5856). For every l μg/g creatinine increment of urinary cadmium, the risk of diabetes increased by 16% (1.16, 95% CI 1.08, 1.25). This meta-analysis suggests that cadmium exposure might be significantly associated with prevalence of diabetes, but large prospective studies are needed to confirm this finding.
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Introduction
Diabetes is now a disease of major concern both globally and regionally and is a serious and increasing global health burden (Zimmet et al. 2014). In 2013, it is estimated that 382 million people had diabetes worldwide, and by 2035, this was predicted to rise to 592 million (Guariguata et al. 2014). Diabetes, similar to most complex traits, regulated by both genetic and environmental factors with the interaction between them (Hu 2011). While many environmental factors associated with diabetes risk have been identified, such as diet, smoking, physical activity, and so on (Bozorgmanesh et al. 2011; Derakhshan et al. 2014), there are as yet some other unknown factors.
Cadmium is a toxic and highly persistent carcinogenic metal occurring in the environment naturally and as a pollutant emanating from industrial and agricultural production, for example nickel-cadmium batteries, fertilizers, coatings, and plastic stabilizers (Jarup and Akesson 2009; Tellez-Plaza et al. 2013). Because of its high rates of soil-to-plant transfer, cadmium is a contaminant found in most human foodstuffs (Clemens 2006). Besides, tobacco smoking, house dust, and cadmium-polluted air are other important sources of cadmium exposure (Jarup and Akesson 2009). Cadmium has a long biological half-life, and only a small fraction of inhaled or ingested cadmium is excreted, resulting in increasing body burden over time (Klaassen 1981; Satarug et al. 2011). With chronic exposure, cadmium influences several human organ systems, such as the kidney, liver, lung, bone, testis, cardiovascular, nervous systems, and so on (Chen et al. 2009a; Hwangbo et al. 2011; Jaishankar et al. 2014; Jomova and Valko 2011).
Diabetogenic effects of cadmium have been suggested in previous animal studies (Edwards and Prozialeck 2009; Lei et al. 2007). However, the results from population-based studies on urinary cadmium and diabetes risk remain controversial. Some supported that urinary cadmium was associated with increased risk of diabetes (Schwartz et al. 2003; Son et al. 2015), while others found no statistically significant relationship (Feng et al. 2015; Liu et al. 2015; Menke et al. 2016; Son et al. 2015; Swaddiwudhipong et al. 2010a; Swaddiwudhipong et al. 2010b).
Therefore, we conducted a meta-analysis to (1) assess the risk of diabetes for urinary cadmium, (2) evaluate the possible dose-response relationship of urinary cadmium with diabetes, and (3) explore the heterogeneity among studies and potential small-study effect.
Materials and methods
Search strategy
We systematically searched PubMed, Web of Science, China National Knowledge Infrastructure, and Wanfang Data for relevant available articles published in English or Chinese updated on 21 June 2016, using predefined keywords “cadmium” and “diabetes” (or “diabetes mellitus” or “type 2 diabetes mellitus” or “T2DM”). Moreover, we reviewed the reference lists of retrieved articles to find other potentially relevant studies.
Inclusion criteria
The inclusion criteria are as follows: (1) cohort, case-control, or cross-sectional study published as an original study; (2) the exposure of interest was urinary cadmium concentration; (3) the outcome of interest was diabetes; (4) multivariate-adjust hazard ratio (HR), relative risk (RR), or odds ratio (OR) with corresponding 95% confidence interval (CI) were reported; and (5) the most recent and complete article was chosen if data from the same population had been published more than once.
Two investigators reviewed all studies independently. If they disagreed with the eligibility of an article, they discussed it with a third investigator to resolve it.
Data extraction
The following data was extracted from each study: the first author’s name, publication year, country in which the study was performed, population-based, study design, sample size and number of cases, gender distribution, age range and mean age, methods to assess diabetes, cutoff values for each category of urinary cadmium concentration, and the OR with 95% CI (adjusted by the most confounders in the original studies) for each category of urinary cadmium.
For dose-response analysis, the number of cases and participants and OR (95% CI) for each level of urinary cadmium were extracted. The median or mean level of urinary cadmium for each level was assigned to the corresponding OR for every study. If the upper boundary of the highest level was not provided, we assumed that the boundary had the same amplitude as the adjacent level. We extracted ORs that reflected the greatest degree of control for potential confounders.
Statistical analysis
Pooled measure was calculated as the inverse variance-weighted mean of the natural logarithm of multivariate-adjusted ORs with 95% CIs to assess the association of urinary cadmium with diabetes. The I 2 was used to assess heterogeneity among studies (I 2 values of 0, 25, 50, and 75% represent no, low, moderate, and high heterogeneity, respectively) (Higgins and Thompson 2002). The fixed effect model (FEM) was used as the pooling method if moderate or lower heterogeneity (I 2 < 50%) was found; otherwise, the random effect model (REM) was adopted (Higgins et al. 2003). Meta-regression with restricted maximum likelihood estimation (JP et al.) was performed to assess the potentially important covariates that might exert substantial impacts on between-study heterogeneity. Subgroup analysis by the continent was conducted. An analysis of influence was conducted with one study removed at a time to assess whether the results could have been affected markedly by a single study. Small-study effect was estimated using Egger’s regression asymmetry test (Egger et al. 1997) and via visual inspection of the funnel plot.
For dose-response analysis, a two-stage, random-effect, dose-response meta-analysis (Orsini et al. 2012) was performed. In the first stage, a restricted cubic spline model with three knots at the 10th, 50th, and 90th percentiles (Harrell et al. 1988) of the levels of urinary cadmium was estimated using generalized least square regression, taking into account the correlation within each set of published OR (Harrell et al. 1988). Then, the study-specific estimates were combined using the restricted maximum likelihood method in a multivariate random-effect meta-analysis (Jackson et al. 2010). A p value for nonlinearity was calculated by testing the null hypothesis that the coefficient of the second spline is equal to 0.
All statistical analyses were performed with STATA version 12.0 (Stata Corporation, College Station, TX, USA). All reported probabilities (p values) were two-sided, with p ≤ 0.05 considered statistically significant.
Results
Literature search and study characteristics
The detailed steps of the literature search are shown in Fig. 1. We identified seven cross-sectional articles (Feng et al. 2015; Liu et al. 2015; Menke et al. 2016; Schwartz et al. 2003; Son et al. 2015; Swaddiwudhipong et al. 2010a; Swaddiwudhipong et al. 2010b) eligible for this meta-analysis on the relationship of urinary cadmium with diabetes risk, including 28,691 participants. Among the seven articles, two articles (Son et al. 2015; Swaddiwudhipong et al. 2010b) reported separate outcomes by males and females. Thus, there were nine studies included in this meta-analysis. With regard to the study region, seven studies (Feng et al. 2015; Liu et al. 2015; Son et al. 2015; Swaddiwudhipong et al. 2010a; Swaddiwudhipong et al. 2010b) were conducted in Asia and two studies were in America (Menke et al. 2016; Schwartz et al. 2003). The baseline characteristics of the study design and participants in the published articles are shown in Table 1.
Quantitative synthesis
Among nine studies, two studies reported positive associations with statistical significance between urinary cadmium and diabetes for the highest versus lowest level of urinary cadmium; the rest seven studies found no significant results. The pooled OR in fixed-effect model was 1.02 (95% CI 1.00, 1.05; I 2 = 42.3%; P heterogeneity = 0.085) (Fig. 2).
When we stratified the analysis by geographical region, the ORs were 1.02 (95% CI 1.00, 1.05; I 2 = 0.9%; P heterogeneity = 0.417) for studies conducted in Asia and 1.11 (95% CI 0.88, 1.41; I 2 = 86.3%; P heterogeneity = 0.007) in America (Fig. 3).
For dose-response analysis, data from studies (Feng et al. 2015; Liu et al. 2015; Schwartz et al. 2003; Son et al. 2015) including 1736 diabetes cases were used. A linear relationship was found between urinary cadmium and diabetes risk (P for nonlinear = 0.5856), and the ORs (95% CIs) of diabetes were 1.00 (1.00–1.00), 1.03 (0.99–1.06), 1.09 (1.00–1.18), 1.44 (1.15–1.8), and 1.89 (1.10–3.25) for 0.26, 0.5, 0.93, 2.4, and 3.6 μg/g creatinine. In addition, every l μg/g creatinine increment of urinary cadmium, the risk of diabetes increased by 16% (1.16, 95% CI 1.08, 1.25) (Fig. 4).
Sources of heterogeneity
Evidence of mild heterogeneity among studies was demonstrated for urinary cadmium with diabetes in Fig. 2. Univariate meta-regression analysis showed that p values for covariates of sample size, year, continent, and number of cases were 0.860, 0.307, 0.624, and 0.519, respectively, which indicated that no covariates had a significant impact on between-study heterogeneity.
Influence analysis
All of the point estimates lay within the 95% CI of the combined analysis, indicating that no individual study had excessive influence on the pooled effect between risk of urinary cadmium and diabetes.
Small-study effect evaluation
The visual inspection of the funnel plot and Egger test showed no evidence of significant small-study effect for the analysis between urinary cadmium and diabetes (p = 0.242; Fig. 5).
Discussion
This meta-analysis including 28,691 participants found a positive association between urinary cadmium concentrations, as a biomarker of long-term cadmium exposure, and diabetes. The further dose-response analysis including 1736 diabetes cases identified that each 1 μg/g creatinine increment of urinary cadmium concentration was associated with a 16% increased risk of diabetes.
It has generally been accepted that urinary cadmium is an appropriate indicator to reflect the body burden of cadmium, especially at relatively low exposure level, while blood cadmium concentration mainly reflect current exposure due to its short half-life (approximately 2–3 months) (Berglund et al. 1994; Hoffmann et al. 2001; Welinder et al. 1977). The mechanisms underlying the association between urinary cadmium and diabetes are still not fully understood. Several potential mechanisms exist by which cadmium exposure might increase the risk of diabetes. Frist, cadmium exposure may cause diabetic symptoms through increasing of the insulin resistance and disruption of β cell function (Chen et al. 2009b). Second, many studies indicated that cadmium toxicity was correlated with increased reactive oxygen formation and depletion of antioxidants, resulting in an oxidative stress (Kukongviriyapan et al. 2016). It is generally agreed upon that oxidative stress plays important roles in the disease of diabetes (Kreuz and Fischle 2016). Third, an animal study with mice has suggested that exposure to cadmium may induce unusually small adipocytes and modulate the expression of adipokines differently from the case of physiologically small adipocytes and may accelerate the risk of developing insulin resistance and diabetes (Kawakami et al. 2013). Last, the liver and kidney are target organs for the toxic effects of cadmium, while these two organs are central to the maintenance of blood glucose levels (Satarug and Moore 2012).
Between-study heterogeneity is common in meta-analysis (Munafo and Flint 2004). In this study, mild heterogeneity was found between urinary cadmium and diabetes. Thus, we used meta-regression to explore the potentially important causes for between-study heterogeneity. Meta-regression did not find the covariates of sample size, year, continent, and number of cases as the important contributors to the heterogeneity. In the subgroup analysis stratified by the continent, we found that the pooled OR of diabetes with urinary cadmium exposure was still significant, whereas the I 2 declined to 0.9% in Asia, indicating that the continent across studies contributed to the heterogeneity.
A major strength of this study was that the large number of participants, reducing sampling error to a great extent, allowed a much greater possibility of reaching reasonable conclusions. An additional strength is that dose-response analysis was conducted to explore the relationships between urinary cadmium and the risk of diabetes quantitatively. Last, all results were consistent, which indicated that the results were not affected by small-study effects.
Nevertheless, our meta-analysis has several limitations. First, most of the studies we have included were cross-sectional design. Hence, we cannot rule out the possibility that the positive association observed between cadmium exposure and diabetes risks in the current studies reflects the effects of treatment for diabetes or the disease itself on urinary cadmium (reverse causation). Second, there was potential problem not be able to solved in the meta-analysis with confounding that could be inherent in the original studies. Inadequate adjustment for confounders could have resulted in exaggeration or underestimation of true association between urinary cadmium and diabetes. Third, the pooled OR of diabetes for the highest versus lowest level of urinary cadmium was borderline significant; more studies are needed to confirm this finding.
Conclusions
In summary, results from this meta-analysis indicated that high cadmium exposure, as measured by urinary cadmium, was a significant risk factor of diabetes. Considering that all of the studies involved in our meta-analysis were cross-sectional design, the effect of cadmium exposure upon prevalence of diabetes should be further verified in large prospective studies.
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This work was supported by grants from the National Natural Science Foundation of China (81302485) and the Natural Science Foundation of Shandong Province (BS2013SF002).
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Yujie Li and Yun Zhang have equal contributions.
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Li, Y., Zhang, Y., Wang, W. et al. Association of urinary cadmium with risk of diabetes: a meta-analysis. Environ Sci Pollut Res 24, 10083–10090 (2017). https://doi.org/10.1007/s11356-017-8610-8
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DOI: https://doi.org/10.1007/s11356-017-8610-8