Summary
Peroxidases are essential enzymes in biodegradation of lignin and lignite which have been investigated intensively in the white-rot fungi. This is the first report of purification and characterization of lignin peroxidase from Penicillium sp. P6 as lignite degradation fungus. The results indicated that the lignin peroxidase of Penicillium decumbens P6 had physical and chemical properties and a N-terminal amino acid sequence different from the lignin peroxidases of white-rot fungi. The lignin peroxidase was isolated from a liquid culture of P. decumbens P6. This enzyme had a molecular weight of 46.3 KDa in SDS-PAGE and exhibited greater activity, temperature stability and wider pH range than those previously reported. The isolation procedure involved (NH4)2SO4 precipitation, ion-exchange chromatography on DEAE-cellulose and CM-cellulose, gel filtration on Sephadex G-100, and non-denaturing, discontinuous polyacrylamide gel electrophoresis. The K m and V max values of this enzyme using veratryl alcohol as substrate were 0.565 mmol L −1 and 0.088 mmol (mg protein) −1 min −1 respectively. The optimum pH of P6 lignin peroxidase was 4.0, and 70.6 of the relative activity was remained at pH 9.0. The optimum temperature of the enzyme was 45 °C.
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References
E. Adler (1977) ArticleTitleLignin chemistry - past, present and future Wood Science and Technology 11 69–218 Occurrence Handle10.1007/BF00365615
R.A. Blanchette (1991) ArticleTitleDelignification by wood decay fungi Annual Review of Phytopathology 29 381–398 Occurrence Handle10.1146/annurev.py.29.090191.002121
D.L. Crawford A.L.III. Pometto R.L. Crawford (1983) ArticleTitleLignin degradation by Streptomyces viridosporous: isolation and characterization of new polymeric lignin degradation intermediate Applied and Environmental Microbiology 45 898–904
N. Duran I. Ferrer J. Rodriguez (1987) ArticleTitleLigninases from Chrysonilia sitophila (TFB-27441) Applied Biochemistry and Biotechnology 16 157–167
R.A. Durie B.M. Lynch S. Strenhell (1960) ArticleTitleComparative studies of brown coal and lignin Australian Journal of Chemistry 13 156–168
R.M. Fakoussa (1992) ArticleTitleMikroorganismen erschlie β en Kohle-Ressourcen Bioengineering 4 21–28
R.M. Fakoussa M. Hofrichter (1999) ArticleTitleBiotechnology and microbiology of coal degradation Applied Microbiology and Biotechnology 52 25–40 Occurrence Handle10.1007/s002530051483 Occurrence Handle10461367
R.L. Farrell K.E. Murtagh M. Tien M.D. Mozuch T.K. Kirk (1989) ArticleTitlePhysical and enzymatic properties of lignin peroxidase isoenzymes from Phanerochaete chrysosporoium Enzyme and Microbial Technology 11 322–328 Occurrence Handle10.1016/0141-0229(89)90014-8
J. Gaskell P. Stewart P.J. Kersten S.F. Covert J. Reiser D. Cullen (1994) ArticleTitleEstablishment of genetic linkage by allelc-specific polymerase chain reaction: application to the lignin peroxidase gene family of Phanerochaete chrysosporium Biotechnology 12 1372–1375 Occurrence Handle10.1038/nbt1294-1372 Occurrence Handle7765568
J.K. Glenn M.A. Morgan M.B. Mayfield (1983) ArticleTitleAn extracellular H 2 O 2 -requiring enzyme preparation involved in lignin biodegradation by the white-rot basidiomycete Phanerochaete chrysosporium Archives of Biochemistry and Biophysics 242 329–341 Occurrence Handle10.1016/0003-9861(85)90217-6
T. Glumoff P.J. Harvey S. Molinari M. Goble G. Frank J.M. Palmer M.S.A. Leisola (1990) ArticleTitleLignin peroxidase from Phanerochaete chrysosporium-molecular and kinetic characterizartion of isozymes European Journal of Biochemistry 187 515–520 Occurrence Handle10.1111/j.1432-1033.1990.tb15333.x Occurrence Handle2303054
F. Guillén A.T. Martínez M.J. Martínez (1992) ArticleTitleSubstrate specificity and properties of the aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii European Journal of Biochemistry 209 603–611 Occurrence Handle10.1111/j.1432-1033.1992.tb17326.x Occurrence Handle1425667
A. Gutiérrez L. Caramelo A. Prieto M.J. Martínez A.T. Martínez (1994) ArticleTitleAnisaldehyde production and aryl-alcohol oxidase and dehydrogenase activities in ligninolytic fungi of the genus Pleur- otus Applied and Environmental Microbiology 60 1783–1788 Occurrence Handle8031078
Y.J. Guo (1991) ArticleTitleThe progress of SDS electrophoresis technology Progress in Biochemistry and Biophysics 18 32–37
P.G. Hatcher (1990) ArticleTitleChemical structural models for coalified wood (vitrinite) in low rank coal Organic and Geochemistry 16 959–970 Occurrence Handle10.1016/0146-6380(90)90132-J
R. Hayatsu R.E. Winans R.L. Mcbeth R.G. Scott L.P. Moore M.H. Studier (1979) ArticleTitleLignin-like polymers in coal Nature 278 41–43
A. Kantelinen A. Hatakka A. Viikari (1989) ArticleTitleProduction of lignin peroxidase and laccase by Phlebia radiate Applied Microbioogy andl Biotechnology 31 234–239
T.K. Kirk R.L. Farrell (1987) ArticleTitleEnzymatic combustion: the microbial degradation of lignin Annual Review of Microbiology 41 465–505 Occurrence Handle10.1146/annurev.mi.41.100187.002341 Occurrence Handle3318677
M. Kumari R.S. Yadav K.D. Yadav (2002) ArticleTitleSecretion of ligninperoxidase by Penicillium citrinum, Fusarium oxysporum and Aspergillus terreus Indian Journal of Experimental Biology 40 802–806 Occurrence Handle12597550
M. Kuwahara J.K. Glenn M.A. Morgan M.H. Gold (1984) ArticleTitleSeparation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium FEBS Letters 169 247–250 Occurrence Handle10.1016/0014-5793(84)80327-0
F. Laborda I.F. Monistrol N. Luna M. Fernández (1999) ArticleTitleProcesses of liquefaction/solubilization of Spanish coals by microorganisms Applied Microbiology and Biotechnology 52 49–56 Occurrence Handle10.1007/s002530051485 Occurrence Handle10461369
R.T. Lamar M.J. Larsen T.K. & Kirk (1990) ArticleTitleSensitivity to and degradation of pentachlorophenol by Phanerochaete sp Applied and Environmental Microbiology 56 3519–3526
M. Ramachandra D.L. Crawford G. Hertel (1988) ArticleTitleCharacterization of an extracellular lignin peroxidase of the lignocellulolytic Actinomycete Streptomyces Viridosporus Applied and Environmental Microbiology 12 3057–3063
M. Ramachandra D.L. Crawford A.L.III. Pometto (1987) ArticleTitleExtracellular enzyme activities during lignocellulose degradation by Streptomyces spp.: A comparative study of wild-type and genetically manipulated strains Applied and Environmental Microbiology. 12 2754–2760
N. Rothschild C. Novotný V. Šašek C.G. Dosoretz (2002) ArticleTitleLigninolytic enzymes of the fungus Irpex lacteus (Polyporus tulipiferae) isolation and characterization of lignin peroxidase Enzyme and Microbial Technology 31 627–633 Occurrence Handle10.1016/S0141-0229(02)00171-0
M. Tien T.K. Kirk (1984) ArticleTitleLignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2 –requiring oxygenase Proceedings of the National Academy of Sciences, USA 81 2280–2284
J.S. Yang (2004) Purification and characterication of lignin peroxidases from lignite degradation strain-Penicillium decumbens P6. Ph.D Thesis CAU Beijing, China
J.S. Yang H.L. Yuan W.X. Chen (2004) ArticleTitleStudies on extracellular enzyme of lignite degrading fungi-Penicillium sp P6 China Environmental Science 24 24–27 Occurrence Handle10.1360/03wd0455
H.L. Yuan Y.Q. Cai X.G. Zhou W.X. Chen (1999) ArticleTitleBreeding of lignite degrading fungi and analysis of the degraded products Chinese Journal of Applied and Environmental Biology 5 21–24
H.L. Yuan Y.Q. Cai X.G. Zhou W.X. Chen (2000) ArticleTitleStudy on chemical properties of humic acid of microbial degraded lignite Environmental Chemistry 19 240–243
H.L. Yuan F.Q. Wang B.Z. Li W.X. Chen (2002) ArticleTitleBiological activation of humic acid derived from lignite degraded by microorganism Acta Pedologica Sinica 39 129–134
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Yang, J., Yuan, H., Wang, H. et al. Purification and characterization of lignin peroxidases from Penicillium decumbens P6. World J Microbiol Biotechnol 21, 435–440 (2005). https://doi.org/10.1007/s11274-004-1876-2
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DOI: https://doi.org/10.1007/s11274-004-1876-2