Abstract
In veal calf production plant-based proteins are frequently included in milk replacer fed to the animals. Since soy products, which are mostly used, are known for their high levels of phyto-oestrogens, the effects of these feeds on the veal calf prostate were examined. Goal was to determine whether these compounds could interfere with histological screening for oestrogenic growth promoters. In a feeding experiment, four groups of veal calves fed plant-based protein-supplemented milk replacer (PBM), containing 5% soy concentrate, 5% soy isolate, 5% wheat gluten and 2% potato protein, for 4 weeks were compared to animals fed dairy-based control feed (DBM); animals treated with estradiol benzoate, diethylstilbestrol and ethinylestradiol served as positive controls. Daidzein and genistein levels measured in feed and urine showed high levels of genistein and daidzein in the soy isolate and soy concentrate supplemented feeds. Genistein and daidzein were also found in the urine of the animals that were fed these feeds. Haematoxylin–eosin-stained prostate sections of PBM-fed animals showed slight hyperplasia and some dilated tubules as compared to the DBM-fed group, but no metaplasia, which is used for screening for oestrogenic hormones. The positive controls showed extensive squamous metaplasia. Immunohistochemical staining for cytokeratin 5 (using RCK 103 monoclonal antibody) in basal cells showed a normal staining pattern of basal cells in the DBM-fed calves and extensive basal cell proliferation and squamous metaplasia in the oestrogen-treated positive control animals. PBM-fed calves showed no increase of basal cell staining but showed elongations of the basal cells in most animals, sometimes resulting in circular figures. It is concluded that the feeds examined in this study did not interfere with histological screening for oestrogens in male veal calves.
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Abbreviations
- DBM:
-
dairy-based control feed
- GC-MS:
-
gas chromatography–mass spectrometry
- HE:
-
haematoxylin and eosin
- HPLC:
-
high-performance liquid chromatography
- MAT:
-
matairesinol
- ORRA:
-
oestrogen radioreceptor assay
- PBM:
-
plant-based protein-supplemented milk replacer
- SECO:
-
secoisolariciresinol
References
Adams, N.R., 1989. Phytoestrogens. In: P.R. Cheeke (ed.), Toxicants of Plant Origin. Phenolics, vol. IV, (CRC Press, Boca Raton, FL), 23
Adams, N.R., 1995. Detection of the effects of phytoestrogens on sheep and cattle. Journal of Animal Science, 73, 1509–1515
Adler, J.H. and Trainin, D., 1960. A hyperoestrogenic syndrome in cattle. Refuah Veterinarith, 7, 115
Arts J.M., van Baak, M.J., Elliot, C.J., Hewitt, S.A., Cooper, J., van de Velde-Fase, K. and Witkamp, R.F., 1998. Comparison of conventional immunoassays and the oestrogen radioreceptor assay for screening for the presence of oestrogenic anabolic compounds in urine samples. The Analyst, 123, 2579–2583
Axelson, M., Sjovall, J., Gustafsson, B.E. and Setchell, K.D., 1982. Origin of lignans in mammals and identification of a precursor from plants. Nature, 298, 659–660
Barnes, S., Coward, L., Kirk, M. and Sfakianos, J., 1998. HPLC–mass spectrometry analysis of isoflavones. Proceedings of the Society Experimental Biolology and Medicine, 217, 254–262
Biolatti, B., Donn, A., Bollo, E., Brusa, F. and Negro, M., 1994. Pathologic effects following the use of anabolics in veal calves: an update. Proceedings 18th World Buiatric Congress, vol. 2
Biolatti, B., Cabassi, E., Rosmini, R., Groot, M., Castagnaro, M., Benevelli, R., Gilioli, G., Ghizzinardi, A., Giorgi, P., Mazzini, C., Comellini, F., Alberghini, L., Zancanaro, G., Cannizo, T., Amedeo, S., Poppi, L. and Cantoni, A., 2003. Lo screening istologico nella prevenzione dellúso di anabolizzanti nel bovino. Large Animal Review, 9, 9–19
Bovee, T.F.H., Helsdingen, R.J., Rietjens, M.C.M., Keijer, J. and Hoogenboom, L.A.P., 2004. Rapid yeast estrogen bioassays stably expressing human estrogen receptors α and β, and green fluorescent protein: a comparison of different compounds with both receptor types. Journal of Steroid Biochemistry and Molecular Biology, 91, 99–109
Branco-Pardal, P., Lalles, J.P., Formal, M., Guilloteau, P. and Toullec, R., 1995. Digestion of wheat gluten and potato protein by the preruminant calf: digestibility, amino acid composition and immunoreactive proteins in ileal digesta. Reproduction Nutrition Development, 35, 639–654
Deschamps, J.C., Ott, R.S., McEntee, K., Health, E.H., Heinrichs, R.R., Shanks, R.D. and Hixon, J.E., 1987. Effects of zeranol on some reproductive traits in beef bulls. Dissertation Abstract International, B 45, 87–88
Dixon, R.A., 2004. Phytoestrogens. Annual Review of Plant Biology, 55, 225–261
Finazzi, M., Gemetti, D., Grieco, V. and Bontempi, W., 1993. Reperti istologici ed immunoistochimici delle ghiandole annesse all'apparato genitale in vitelli trattati con promotori di crescita. Argomenti di Patologia Veterinaria, 273–283
Girardi, C., Badino, P., Re, G., Dacasto, M., Biolatti, B., Brusa, F. and Di Carlo, F., 1990. Stato recettoriale e reperti anatomo ed istopatologici del tratto genitale femminile in vitelli a carne bianca. Atti della Società Italiana di Buiatria, XXII, 357–367
Grandmontagne, C., 1986. Modifications histologiques de la prostate du veau traité par les différents anabolisants utilisés en élevage. Revue de Mèdecine Véterinaire, 137, 37–47
Groot, M.J. and Biolatti, B., 2004. Histopathological effects of Boldenone in cattle. Journal of Veterinary Medicine Series A, 50, 1–6
Groot, M.J. and den Hartog, J.M.P., 1990. Histological changes in the genital tract of female veal calves implanted with naturally occurring anabolic steroids. Journal of Veterinary Medicine Series A, 37, 775–786.
Groot, M.J., Frijns, L.H.M. and den Hartog, J.M.P., 1989. Histologisch hormoononderzoek, een praktijkevaluatie. Tijdschrift voor Diergeneeskunde, 114, 315–321
Groot, M.J., Schilt, R., Ossenkoppele, J.S., Berende, P.L.M. and Haasnoot, W., 1998. Combinations of growth promoters in veal calves: consequenses for screening and confirmation methods. Journal of Veterinary Medicine Series A, 45, 425–440
Groot, M.J., Ossenkoppele, J.S. and Bakker, R., 2000. Changes in cytokeratin expression in accessory sex glands of growth promoter treated veal calves: influences of treatment schedule and combination of drugs. European Journal of Veterinary Pathology, 6, 19–24
Gropp, J., Boehncke, E., Schulz, V., von Sandersleben, J., Geisel, O. and Hanichen, T. 1976. Effect of 17-β-oestradiol and trenbolone acetate in different doses on several physiological and morphological parameters (provocation trial). Fortschritte in der Tierphysiologie und Tiernahrung, 6, 33–52
Khodabandehlou, H., Hoffmann, B. and Pallauf, J., 1997. Untersuchungen in Mittelhessen zum Vorkommen von östrogen-wirksamen Inhaltstoffen im Rinderfutter. Deutsches Tierärztliche Wochenschrift, 104, 291–294
Kroes, R., 1970. Estrogen-induced changes in the genital tract of the male calf–-a morphological study, (PhD thesis, Rijksuniversiteit Utrecht)
Kroes, R., Huis in 't Veld, L.G., Schulter, P.L. and Stephany, R.W., 1975. Methods for controlling the appication of anabolics in farm animals. Anabolic Agents in Animal Production, FAO/WHO Symposium Rome, (Georg Thieme, Stuttgart), 192–201
Lindner, H.R., 1976. Occurrence of anabolic agents in plants and their importance. Environmental Quality and Safety Supplement, 5, 151–158
Lothammer, K.H., Grunert, E. and Elghamry M.I., 1970. Fertility disorders in a cattle herd fed with corn- and clover grass-silage containing estrogens. Berliner und Münchener Tierarztliche Wochenschrift, 83, 353–357
Mazur, W. and Adlercreutz, H., 1998. Natural and anthropogenic environmental oestrogens: the scientific basis for risk assessment. Pure and Applied Chemistry, 70, 1759–1776
Montagne, L., Crevieu-Gabriel, I., Toullec, R. and Lalles, J.P., 2003. Influence of dietary protein level and source on the course of protein digestion along the small intestine of the veal calf. Journal of Dairy Science, 86, 934–943
Rosmini, R., 1987. Aggiornamento interpretativo dei reperti istologici nella prostata e nella ghiandola di Bartolino di vitelli macellati. Praxis Veterinaria, 8, 8–10
Schaudinn, B. and Beck, G., 1977. Detecting the use of oestrogens in calves by histological means. Fleishwirtschaft, 57, 1664–1668
Schilt, R., Groot, M.J., Berende, P.L.M., Ramazza, V., Ossenkoppele, J.S., Haasnoot, W., Van Bennekom, E.O., Brouwer, L. and Hooijerink, H., 1998. Pour on application of growth promoters in veal calves: analytical and histological results. The Analyst, 123, 2665–2670
Smith, J.F., Jagusch, K.T., Brunswick, L.F.C. and McGowan, L.T., 1979. Coumestanes in lucerne and ovulation in ewes. New Zealand Journal of Agricultural Research, 22, 411
Toullec, R. and Lallès, J.P., 1995. Digestion dans la caillette et l'intestin greêle. In: R. Jarrige, Y. Ruckebush, C. Demarquilly, M. H. Farce and M. Journet (eds.), Nutrition des ruminants domestiques: ingestion et digestion, (INRA, Paris)
Yuangklang, C., Wensing, T., Van den Broek, L., Jittakhot, S. and Beynen, A.C., 2004. Fat digestion in veal calves fed milk replacers low or high in calcium and containing either casein or soy protein isolate. Journal of Dairy Science, 87, 1051–1056
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Groot, M.J. Effects of Phyto-Oestrogens on Veal Calf Prostate Histology. Vet Res Commun 30, 587–598 (2006). https://doi.org/10.1007/s11259-006-3202-y
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DOI: https://doi.org/10.1007/s11259-006-3202-y