Abstract
The improvement of the induction rate in Citrus anther culture is important for taking practical advantage of the haploid potential in breeding. The influence of polyamines on anther culture of Citrus clementina, cv Nules, with particular attention to the free, soluble and insoluble-conjugated polyamine levels, has been investigated. Putrescine, spermidine and putrescine plus spermidine, were added to the standard induction medium. Before culture, spermidine was the most abundant among the free polyamines detected in anthers. The exogenous supply of either putrescine or spermidine, either independently or combined, effected greater uptake and accumulation of polyamines. The addition of 2 mM spermidine to the medium stimulated gametic embryogenesis in clementine Nules, whereas putrescine did not influence embryo production. Regenerants were mostly tri-haploids; a few doubled-haploids and no haploid plants were obtained.
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Abbreviations
- DAP:
-
Diamino propane
- PAs:
-
Polyamines
- PCA:
-
Perchloric acid
- PGI-1:
-
Phosphoglucoisomerase
- PGM:
-
Phosphoglucomutase
- PUT:
-
Putrescine
- SNK’s test:
-
Student–Newman–Keuls’s test
- SPD:
-
Spermidine
- SPM:
-
Spermine
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Acknowledgements
The authors wish to thank Monika Hofer, Luis Navarro and Oscar Olivares-Fuster for the flow cytometry analyses and Dr. Andrew Carter (Institute of Food Research, Norwich, UK) for the English revision. This work was partially supported by the Italian PRIN 2004 to Maria Antonietta Germanà and by the fellowships of the L’Oréal “for Women in Science” 2004 to Benedetta Chiancone and Annalisa Tassoni.
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Chiancone, B., Tassoni, A., Bagni, N. et al. Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan. Plant Cell Tiss Organ Cult 87, 145–153 (2006). https://doi.org/10.1007/s11240-006-9149-4
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DOI: https://doi.org/10.1007/s11240-006-9149-4