Abstract
Two-dimensional air current speeds in the culture vessel were measured using a tracer-based visualization technique and the effect of the air movement in the culture vessel on the photosynthesis of in vitro potato plantlets was assessed under a photoautotrophic culture condition. The air current speeds inside the vessel were varied by controlling free convection induced by spatial variations of temperatures in the culture vessel. For all conditions examined, upward air currents were observed around the plantlets in the central part of the culture vessel and downward air currents were observed near inside walls in the culture vessel. The upward and downward air currents were restricted by the presence of the plantlet. The upward air current speeds were affected by plantlet size inside the vessel and it was 24, 8 and 4 mm s−1 in culture vessels with no plantlets, a 10-mm-tall plantlet and a 60-mm-tall plantlet cultured inside the vessel, respectively. The upward air current speed was increased by 2 times by increasing wind velocity above the culture vessel from 0.1 to 1.0 m s−1. Placing the black plate on the medium also increased the air current speeds by 1.5 times. The net photosynthetic rates of the plantlets increased from 2.0 to 2.5 μmol m−2 s−1 as the upward air current speed in the culture vessel increased from 2.4 to 8.0 mm s−1. The air current speeds in the culture vessel were significantly slow. Enhancement of the air movement in the culture vessel is important to promote photosynthesis of the in vitro plantlets.
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References
LF Daws AD Penwarden GT Waters (1965) ArticleTitleA visualization technique for the study of air movement in rooms J. Instn. Heat. Vent. Engs. 33 24–30
Y De Y Desjardins A Gosselin (1993a) ArticleTitlePhotosynthetic capacity and effects of forced ventilation on growth of in vitro-cultured strawberry plantlets Acta Hortic. Sinica 20 123–126
Y De A Gosselin Y Desjardins (1993b) ArticleTitleEffects of forced ventilation at different relative humidities on growth, photosynthesis and transpiration of geranium plantlets in vitro Can. J. Plant Sci. 73 249–256
Y Kitaya T Fukuda T Kozai C Kirdmanee (1995) ArticleTitleEffects of light intensity and lighting direction on the photoautotrophic growth and morphology of potato plantlets in vitro Scientia Hortic. 62 15–24 Occurrence Handle10.1016/0304-4238(94)00760-D
Y Kitaya Y Ohmura T Kozai (1997) ArticleTitleMethod for visualization of air currents in plant tissue culture vessels Environ. Control Biol. 35 IssueID2 139–141
Y Kitaya J Tsuruyama M Kawai T Shibuya M Kiyota (2000) Effects of air current on transpiration and net photosynthetic rates of plants in a closed plant production C Kubota C Chun (Eds) Transplant Production in the 21st Century Kluwer Academic Publishers Dordrecht, Netherland 83–90
Y Kitaya M Kawai J Tsuruyama H Takahashi A Tani E Goto T Saito M Kiyota (2001) ArticleTitleThe effect of gravity on surface temperature and net photosynthetic rate of plant leaves Adv. Space Res. 28 659–664 Occurrence Handle10.1016/S0273-1177(01)00375-1 Occurrence Handle11803969
Y Kitaya M Kawai J Tsuruyama T Shibuya M Kiyota (2003a) ArticleTitleEffects on air current speed on the transpiration rate and net photosynthetic rate of plant leaves Adv. Space Res. 31 IssueID1 177–182 Occurrence Handle10.1016/S0273-1177(02)00747-0
Y Kitaya M Kawai J Tsuruyama H Takahashi A Tani E Goto T Saito M Kiyota (2003b) ArticleTitleThe effects of gravity on surface temperatures of plant leaves Plant, Cell and Environment 26 497–503
T Kozai (1991) Autotrophic micropropagation YPS Bajaj (Eds) Biotechnology in Agriculture and Forestry Springer-Verlag N.Y. 313–343
T Kozai N Ohde C Kubota (1991) ArticleTitleSimilarity of growth patterns between plantlets and seedlings of Brassica campestris L. under different in vitro environmental conditions Plant Cell Tiss. Org. Cult. 24 181–86 Occurrence Handle10.1007/BF00033474
T Kozai K Fujiwara M Hayashi J Aitken-Christie (1992) The in vitro environment and its control in micropropagation K Kurata T Kozai (Eds) Transplant Production Systems Kluwer Academic Publishers Dordrecht 247–282
T Kozai MAL Smith (1994) Environmental control in plant tissue culture J Aitken-Christie T Kozai MAL Smith (Eds) Automation and Environmental Control in Plant Tissue Culture Kluwer Academic Publishers Dordrecht 301–318
C Kubota T Kozai (1992) ArticleTitleGrowth and net photosynthetic rate of Solanum tuberosum in vitro under forced and natural ventilation HortScience 27 1312–1314
JL Monteith MH Unsworth (1990) Principles of Environmental Physics Edward and Arnold Publishing Co London 101–120
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tabacco tissue cultures Physiol. Plant. 15 473–497
M Nakayama T Kozai K Watanabe (1991) ArticleTitleEffect of the presence/absence of the sugar in the medium and natural forced ventilation on the net photosynthetic rates of potato explants in vitro Plant Tiss. Cult. Lett. 8 105–109
QT Nguyen T Kozai J Heo DX Thai (2001) ArticleTitlePhotoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched conditions Plant Cell Tiss. Org. Cult. 66 217–225 Occurrence Handle10.1023/A:1010662413486
K Ohyama T Kozai (1997) ArticleTitleCO2 concentration profiles in a plant tissue culture vessel Environ. Contr. Biol. 35 IssueID3 197–202
KE Torrance L Orloff JA Rockett (1969) ArticleTitleExperiments on natural convection enclosures with localized heating from below J. Fluid Mech. 36 21–31
K Yabuki H Miyagawa (1970) ArticleTitleStudies on the effect of wind speed upon the photosynthesis (2) Japan. J. Agric. Met. 26 137–141
SMA Zobayed F Afreen C Kubota T Kozai (2000) ArticleTitleWater control and survival of Ipomoea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilation Ann. Bot. 86 603–610 Occurrence Handle10.1006/anbo.2000.1225
SMA Zobayed J Armstrong W Armstrong (2001) ArticleTitleLeaf anatomy of in vitro tobacco and cauliflower plantlets as affected by different types of ventilation Plant Sci. 161 537–548 Occurrence Handle10.1016/S0168-9452(01)00438-1
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Kitaya, Y., Ohmura, Y., Kubota, C. et al. Manipulation of the culture environment on in vitro air movement and its impact on plantlets photosynthesis. Plant Cell Tiss Organ Cult 83, 251–257 (2005). https://doi.org/10.1007/s11240-005-6839-2
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DOI: https://doi.org/10.1007/s11240-005-6839-2