Abstract
Related or distant species of cultivated cs are a large pool of many desirable genes. Gene transfer from these species through conventional breeding is difficult owing to post- and pre-zygotic sexual incompatibilities. Somatic hybridization via protoplast fusion is a possible alternative for gene transfer from these species to cultivated crops. Since the early days of somatic hybridization many intergeneric somatic hybrids have been developed through symmetric fusion, asymmetric fusion and microfusion. Somatic hybrids are mainly selected by using markers such as specific media or fusion parents with special features, biochemical mutants, antibiotic resistance and complementation strategy. The hybridity of the regenerants is determined based on morphological, cytological and molecular analysis. The inheritance patterns of nuclear and cytoplasmic genomes in the somatic hybrids are diverse. Nuclear DNA from both fusion parents co-exists congruously in some hybrids with translocation and rearrangement of chromosomes, but spontaneous elimination of chromosomes from either or both fusion parents has been observed very often. In asymmetric fusion, chromosome elimination is an important issue that is a complicated process influenced by many factors, such as irradiation dose, phylogenetic relatedness, ploidy level of fusion parent and regenerants. As for chloroplast genome, uniparental segregation is mainly detected, though co-existence is also reported in some cases. The mitochondrial genome, in contrast to chloroplast, undergoes recombination and very frequent rearrangements. Somatic cell fusion has potential applications for crop genetic improvement by overcoming sexual incompatibility or reproductive barriers, and by realizing novel combinations of nuclear and/or cytoplasmic genomes.
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PS Ahuja LU Rahman SC Bhargava S Banerjee (1993) ArticleTitleRegeneration of intergeneric somatic hybrid plants between Atropa belladonna L. and Hyoscyamus muticus L Plant Sci. 92 91–98
SNH Ali DJ Juigen MS Ramanna E Jacobsen RGF Visser (2000) ArticleTitleGenomic in situ hybridization analysis of a trigenomic hybrid involving Solanum and Lycopersicon species Genome 44 299–304
SNH Ali MS Ramanna E Jacobsen RGF Visser (2001) ArticleTitleEstablishment of a complete series of a monosomic tomato chromosome addition lines in the cultivated potato using RFLP and GISH analyses Theor. Appl. Genet. 103 687–695
N Arumugam A Mukhopadhyay V Gupta YS Sodhi JK Verma D Pental AK Pradhan (2002) ArticleTitleSynthesis of somatic hybrids (RCBB) by fusing heat-tolerant Raphanus sativus (RR) and Brassica oleracea (CC) with Brassica nigra (BB) Plant Breed. 121 168–170
E Babiychuk S Kushnir YY Gleba (1992) ArticleTitleSpontaneous chromosome elimination in somatic hybrids between somatically congruent species Nicotiana tabacum L and Atropa belladonna L Theor. Appl. Genet. 84 87–91
A Baldev K Gaikwad PB Kirti T Mohapatra S Prakash V Chopra (1998) ArticleTitleRecombination between chloroplast genomes of Trachystoma ballii and Brassica juncea following protoplast fusion Mol. Gen. Genet. 260 357–361
B Bauer-Weston W Keller J Webb S Gleddie (1993) ArticleTitleProduction and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus Theor. Appl. Genet. 86 150–158
F Begum S Paul N Bag SR Sikdar SK Sen (1995) ArticleTitleSomatic hybrids between Brassica juncea (L.) Czern. and Diplotaxis harra (Forsk.) Boiss and the generation of backcross progenies Theor. Appl. Genet. 91 1167–1172
M Bellaoui A Martin-Canadell G Pelletier F Budar (1998) ArticleTitleLow-copy-number molecules are produced by recombination, actively maintained and can be amplified in the mitochondrial genome of Brassicaceae: relationship to reversion of the male sterile phenotype in some cybrids Mol. Gen. Genet. 257 177–185
H Binding R Nehls (1978) ArticleTitleSomatic cell hybridization of Vicia faba (broad beans) + Petunia hybrida Mol. Gen. Genet. 164 137–143
S Bohman J Forsberg K Glimelius C Dixelius (1999) ArticleTitleInheritance of Arabidopsis DNA in offspring from Brassica napus and A. thaliana somatic hybrids Theor. Appl. Genet. 98 99–106
S Bohman M Wang C Dixelius (2002) ArticleTitleArabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background Theor. Appl. Genet. 105 498–504
S Bonhomme F Budar D Lancelin I Small MC Defrance G Pelletier (1992) ArticleTitleSequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids Mol. Gen. Genet. 235 340–348
HT Bonnett K Glimelius (1990) ArticleTitleCybrids of Nicotiana tabacum and Petunia hybrida have an intergeneric mixture of chloroplasts from P. hybrida and mitochondria identical or similar to N. tabacum Theor. Appl. Genet. 79 550–555
EP Brewer JA Saunders JS Angle RL Chaney MS McIntosh (1999) ArticleTitleSomatic hybridization between the zinc accumulator Thlaspi caerulescens and Brassica napus Theor. Appl. Genet. 99 761–771
F Budar P Touzet R Paepe Particlede (2003) ArticleTitleThe nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited Genetica 117 3–16
PS Carlson HH Smith RD Dearing (1972) ArticleTitleParasexual plant hybridization Proc. Natl. Acad. Sci. USA 69 2292–2294
C Chatterjee S Sikdar S Das SK Sen (1988) ArticleTitleIntergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis Theor. Appl. Genet. 79 915–922
K Chen SG Wildman HH Smith (1977) ArticleTitleChloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein Proc. Natl. Acad. Sci. USA 74 5109–5112
SY Chen GM Xia HM Chen SP Feng (2000) ArticleTitleStudies on NaCl-tolerance among somatic hybrid lines of wheat and Agropyron elongatum with their parents (in Chinese) Acta. Bot. Boreat. Occident. Sin. 20 327–332
WP Chen QS Wu DJ Liu (1992) ArticleTitleCallus formation from somatic hybridization of wheat (Triticum aestivum L.) and ryegrass (Lolium perenne L.) by electrofusion (in Chinese) Acta. Bot. Sin. 34 284–290
YJ Cheng WW Guo XX Deng (2003) ArticleTitleMolecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal Valencia orange (Citrus sinensis) + Meiwa Kumquat (Fortunella crassifolia) intergeneric somatic hybrids Plant Cell Rep. 21 445–451
AX Cheng GM Xia (2004) ArticleTitleSomatic hybridization between common wheat and Italian ryegrass Plant Sci. 166 1219–1226
AX Cheng GM Xia DY Zhi HM Chen (2004) ArticleTitleIntermediate fertile Triticum aestivum (+) Agropyron elongatum somatic hybrids are generated by low doses of UV irradiation Cell Res. 14 86–91
AM Chevre F Eber E Margale MC Kerlan C Primard F Vedel M Delseny G Pelletier (1994) ArticleTitleComparison of somatic and sexual Brassica napus–Sinapis alba hybrids and their progeny by cytogenetic studies and molecular characterization Genome 37 367–374
YC Chien KN Kao LR Wetter (1982) ArticleTitleChromosomal and isozyme studies of Nicotiana tabacum–Glycine max hybrid cell lines Theor. Appl. Genet. 62 301–304
MAPC Costa BMJ Mendes FAAM Filho (2003) ArticleTitleSomatic hybridization for improvement of citrus rootstock: production of five new combinations with potential for improved disease resistance Aust. J. Exp. Agric. 43 1151–1156
JH De Jong AMA Wolters JM Kok H Verhaar J Van Eden (1993) ArticleTitleChromosome pairing and potential for intergeneric recombination in some hypotetraploid somatic hybrids of Lycopersicon esculentum (+) Solanum tuberosum Genome 36 1032–1041
XX Deng JW Grosser FG Gmitter SuffixJr (1992) ArticleTitleIntergeneric somatic hybrid plants from protoplast fusion of Fortunella crassifolia cultivar ‘Meiwa’ with Citrus sinensis cultivar ‘Valencia’ Sci. Hortic. 49 55–62
A Dragoeva I Attanasov V Attanasov (1999) ArticleTitleCMS due to tapetal failure in cybrids between Nicotiana tabacum and Petunia hybrida Plant Cell Tiss. Organ Cult. 55 67–70
D Dudits G Fejer C Hadlaezdy C Koncz GB Lazar G Horvath (1980) ArticleTitleIntergeneric gene transfer mediated by plant protoplast fusion Mol. Gen. Genet. 179 283–288
D Dudits E Maroy T Praznovszky Z Olah J Gyorgyey R Cella (1987) ArticleTitleTransfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: regeneration of fertile plants Proc. Natl. Acad. Sci. USA 84 8434–8438
P Durieu SJ Ochatt (2000) ArticleTitleEfficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts J. Exp. Bot. 51 1237–1242
ED Earle M Temple TW Walters (1992) ArticleTitleOrganelle assortment and mitochondrial DNA rearrangements in Brassica somatic hybrids and cybrids Physiol. Plant 85 325–333
T Endo T Komiya M Mino K Nakanishi S Fujita Y Yamada (1988) ArticleTitleGenetic diversity among sublines originating from a single somatic hybrid cell of Duboisia hopwoodii + Nicotiana tabacum Theor. Appl. Genet. 76 641–646
A Escanlante S Imanishi M Hossain N Ohmido K Fukui (1998) ArticleTitleRFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides Theor. Appl. Genet. 96 719–726
J Fahleson I Eriksson K Glimelius (1994) ArticleTitleIntertribal somatic hybrids between Brassica napus and Barbarea vulgaris-production of in vitro plantlets Plant Cell Rep. 13 411–416
J Fahleson I Eriksson M Landgren S Stymme K Glimelius (1994) ArticleTitleIntertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid Theor. Appl. Genet. 87 795–804
J Fahleson L Rahlen K Glimelius (1988) ArticleTitleAnalysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa Theor. Appl. Genet. 76 507–512
RP Finch IH Slamet EC Cocking (1990) ArticleTitleProduction of heterokaryons by the fusion of mesophyll protoplasts of Porteresia coarctata and cell suspension-derived protoplasts of Oryza sativa: a new approach to somatic hybridization J. Plant Physiol. 136 592–598
J Forsberg C Dixelius U Lagercrantz K Glimelius (1998) ArticleTitleUV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana Plant Sci. 131 65–76
J Forsberg U Lagercrantz K Glimelius (1998) ArticleTitleComparison of UV light, X-ray and restriction enzyme treatment as tools in production of asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana Theor. Appl. Genet. 96 1178–1185
J Forsberg M Landgren K Glimelius (1994) ArticleTitleFertile somatic hybrids between Brassica napus and Arabidopsis thaliana Plant Sci. 95 213–223
CH Fu WW Guo JH Liu XX Deng (2003) ArticleTitleRegeneration of Citrus sinensis + Clausena lansium intergeneric triploid and tetraploid somatic hybrids and their molecular identification In Vitro Cell Dev. Biol. Plant 39 360–364
H Furuta H Shinoyama Y Nomura M Maeda K Makara (2004) ArticleTitleProduction of intergeneric somatic hybrids of chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] and wormwood (Artemisia sieversiana J F. Ehrh. ex. Willd) with rust (Puccinia horiana Henning) resistance by electrofusion of protoplasts. Plant Sci. 166 695–702
K Gaikwad PB Kirti A Sharma S Parkash VL Chopra (1996) ArticleTitleCytogenetical and molecular investigations on somatic hybrids of Sinapsis alba and Brassica juncea and their backcross progeny Plant Breed. 115 480–483
F Garriga-Caldere DJ Huigen A Angrisano E Jacobsen MS Ramanna (1998) ArticleTitleTransmission of alien tomato chromosomes from BC1 to BC2 progenies derived from backcrossing potato (+) tomato fusion hybrids to potato: The selection of single additions for seven different tomato chromosomes Theor. Appl. Genet. 96 155–163
F Garriga-Caldere DJ Huigen F Filotico E Jacobsen MS Ramanna (1997) ArticleTitleIdentification of alien chromosomes through GISH and RFLP analysis and the potential for establishing potato lines with monosomic additions of tomato chromosomes Genome 40 666–673
T Gavrilenko N Barbakar A Pavlov (1992) ArticleTitleSomatic hybridization between Lycopersicon esculentum and non-tuberous Solanum species of the Etuberosa series Plant Sci. 86 203–214
T Gavrilenko R Thieme VM Rokka (2001) ArticleTitleCytogenetic analysis of Lycopersicon esculentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants Theor. Appl. Genet. 103 231–239
TM Ge YJ Yu YF Xie DP Zhang RD Zhang FL Qin (1997) ArticleTitleAsymmetric somatic hybridization and plant regeneration between wheat and Italian ryegrass (in Chinese) J. Huazhong Agric. Univ. 16 105–111
LJW Gilissen MJ Staceren Particlevan HA Verhoeven KS Ramulu (1992) ArticleTitleSomatic hybridization between potato and Nicotiana plumbaginifolia 1 Spontaneous biparental chromosome elimination and production of asymmetric hybrids. Theor. Appl. Genet. 84 73–80
YY Gleba S Hinnisdaels VA Sidorov VA Kaleda AS Parokonny NV Boryshuk NN Cherep I Negrutiu M Jacobs (1988) ArticleTitleIntergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by gamma fusion Theor. Appl. Genet. 76 760–766
YY Gleba F Hoffmann (1978) ArticleTitleHybrid cell lines Arabidopsis thaniala + Brassica campestris: no evidence for specific chromosome elimination Mol. Gen. Genet. 165 257–264
YY Gleba F Hoffmann (1980) ArticleTitleArabidobrassica: a novel plant obtained by protoplast fusion Planta 149 112–117
YY Gleba VP Momot NN Cherep MV Skarzynskaya (1982) ArticleTitleIntertribal hybrid cell lines of Atropa belladonna (×) Nicotiana chinensis obtained by cloning individual protoplast fusion products Heterokaryocyte cloning, parasexual hybrids. Theor. Appl. Genet. 62 75–79
K Glimelius HT Bonnett (1986) ArticleTitleNicotiana cybrids with Petunia chloroplasts Theor. Appl. Genet. 72 794–798
JW Grosser JL Chandler (2000) ArticleTitleSomatic hybridization of high yield, cold-hardy and disease resistant parents for citrus rootstock improvement J. Hortic. Sci. Biotech. 75 641–644
JW Grosser JL Chandler (2003) ArticleTitleNew Rootstocks via protoplast fusion Acta Hortic. 622 491–497
JW Grosser FG Gmitter SuffixJr JL Chandler (1988) ArticleTitleIntergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha Theor. Appl. Genet. 75 397–401
JW Grosser FG Gmitter SuffixJr JL Chandler (1988) ArticleTitleIntergeneric somatic hybrid plants of Citrus sinensis cv Hamlin and Poncirus trifoliata cv. Flying Dragon. Plant Cell Rep. 7 5–8
JW Grosser FG Gmitter SuffixJr N Tusa JL Chandler (1990) ArticleTitleSomatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana Plant Cell Rep. 8 656–659
JW Grosser J Jiang ES Louzada JL Chandler FG Gmitter SuffixJr (1998) ArticleTitleSomatic hybridization, an integral component of citrus cultivar improvement: II Rootstock improvement Hortic. Sci. 33 1060–1061
JW Grosser FAA Mourao-Fo FG Gmitter SuffixJr ES Louzada J Jiang K Baergen A Quiros C Cabasson J Schell JL Chandler (1996) ArticleTitleAllotetraploid hybrids between citrus and seven related genera produced by somatic hybridization Theor. Appl. Genet. 92 577–582
JW Grosser P Ollitrault O Olivares-Fuster (2000) ArticleTitleSomatic hybridization in Citrus: an effective tool to facilitate variety improvement In Vitro Cell Dev. Biol. Plant 36 434–449
WW Guo YJ Cheng XX Deng (2002) ArticleTitleRegeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata Plant Cell Rep. 20 829–834
WW Guo XX Deng (1998) ArticleTitleSomatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata Plant Cell Rep. 18 297–300
WW Guo XX Deng (1999) ArticleTitleIntertribal hexaploid somatic hybrid plantlets regeneration between diploids of Citrus sinensis and its sexually incompatible relative Clausena lansium Theor. Appl. Genet. 98 581–585
PP Gupta O Schieder M Gupta (1984) ArticleTitleIntergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion Mol. Gen. Genet. 197 30–35
A Guri LJ Dunbar KC Sink (1991) ArticleTitleSomatic hybridization between selected Lycopersicon and Solanum species Plant Cell Rep. 10 76–80
A Guri A Levi KC Sink (1988) ArticleTitleMorphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum Mol. Gen. Genet. 212 191–198
M Hagimori (1995) ArticleTitleClubroot disease resistance and other characteristics of the progeny of somatic hybrids between Japanese radish and cauliflower Acta Hortic. 392 77–80
M Hagimori M Nagaoka N Kato Yoshikawa (1992) ArticleTitleProduction and characterization of somatic hybrids between the Japanese radish and cauliflower Theor. Appl. Genet. 84 819–824
RD Hall GJA Rouwendal FA Krens (1992) ArticleTitleAsymmetric somatic cell hybridization in plants. I. The early effects of (sub)lethal doses of UV and gamma irradiation on the cell physiology and DNA integrity of cultured sugarbeet (Beta vulgarris L) protoplasts. Mol. Gen. Genet. 234 306–314
RD Hall GJA Rouwendal FA Krens (1992) ArticleTitleAsymmetric somatic cell hybridization in plants. II. Electrophoretic analysis of radiation-induced DNA damage and repair following the exposure of sugarbeet (Beta vulgaris L ) protoplasts to UV and gamma rays. Mol. Gen. Genet. 234 315–324
LW Handley RL Nickels MW Cameron PP Moore KC Sink (1986) ArticleTitleSomatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides Theor. Appl. Genet. 71 691–697
LN Hansen (1998) ArticleTitleIntertribal somatic hybridization between rapid cycling Brassica oleracea L. and Camelina sativa (L.) Crantz Euphytica 104 173–179
LN Hansen ED Earle (1997) ArticleTitleSomatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk) Sacc Theor. Appl. Genet. 94 1078–1085
S Hinnisdaels L Bariller A Mouras V Sidorov J Del-Favero J Vauskens I Negrutiu M Jacobs (1991) ArticleTitleHighly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombination and translocation events in somatic hybrid plants after ‘gamma-fusion’ Theor. Appl. Genet. 82 609–614
F Hoffmann T Adachi (1981) ArticleTitle‘Arabidobrassica’: chromosomal recombination and morphogenesis in asymmetric intergeneric hybrids Planta 153 586–893
K Horsman T Gavrilenko M Bergervoet DJ Huigen ATW Joe E Jacobsen (2001) ArticleTitleAlteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridization: selection of fusion hybrids by DNA measurements and GISH Plant Breed. 120 201–207
M Hossain S Imanishi A Matsumoto (1994) ArticleTitleProduction of somatic hybrids between tomato (Lycopersicon esculentum) and nightshade (Solanum lycopersioides) by electrofusion Breed. Sci. 44 405–412
Q Hu SB Andersen C Dixelius LN Hansen (2002) ArticleTitleProduction of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool Plant Cell Rep. 21 147–152
Q Hu L Hansen J Laursen C Dixelius S Andersen (2002) ArticleTitleIntergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding Theor. Appl. Genet. 105 834–840
Y Huang XL Zhai SS Li GM Xia HM Chen (1999) ArticleTitleIntergeneric somatic hybridization between Triticum aestivum L. and Leymus chinensis (Trin.) Tzvel (in Chinese) Acta Bot. Boreal. Occident Sin. 19 659–654
J Imamura MW Saul I Potrkus (1987) ArticleTitleX-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of the products Theor. Appl. Genet. 74 445–450
S Ishikawa SW Bang Y Kaneko Y Matsuzawa (2003) ArticleTitleProduction and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica oleracea Plant Breed. 122 233–238
E Jacobsen Jong JH Particlede SA Kamstra PMMM Berg Particlevan den MS Ramanna (1995) ArticleTitleGenomic in situ hybridization (GISH) and RFLP analysis for the identification of alien chromosomes in the backcross progeny of potato (+) tomato fusion hybrids Heredity 74 250–257
SM Jain EA Shahin S Sun (1988) ArticleTitleInterspecific protoplast fusion for the transfer of atrazine resistance from Solanum nigrum to tomato (Lycopersicon esculentum L.). Plant Cell Tiss Organ Cult. 12 189–192
NB Jelodar NW Blackhall TPV Hartman DS Brar G Khush MR Davey EC Cocking JB Power (1999) ArticleTitleIntergeneric somatic hybrids of rice [Oryza sativa L. (+) Porteresia coarctata (Roxb) Tateoka] Theor. Appl. Genet. 99 570–577
AAT Johnson RE Veilleux (2001) ArticleTitleSomatic hybridization and applications in plant breeding Plant Breed. Rev. 20 167–225
N Kaimori M Senda R Ishikawa S Akada T Harada M Niizeki (1998) ArticleTitleAsymmetric somatic cell hybrids between alfalfa and birdsfoot trifoil Breed. Sci. 48 29–34
T Kameya H Kanzak S Tori T Abe (1989) ArticleTitleTransfer of radish (Raphanus sativus L.) chloroplast into cabbage (Brassica oleracea L.) by protoplast fusion Jpn. J. Genet. 64 27–34
A Kanno H Kanzaki T Kameya (1997) ArticleTitleDetailed analyses of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage Plant Cell Rep. 16 479–484
KN Kao (1977) ArticleTitleChromosome behavior in somatic hybrids of soybean-Nicotiana glauca Mol. Gen. Genet. 150 225–230
M Kihara KN Cai R Ishikawa T Harada M Niizeki K Saito (1992) ArticleTitleAsymmetric somatic hybrid calli between Leguminous species of Lotus corinculatus and Glycine max and regenerated plants from the calli Jpn. J. Breed. 42 55–64
PB Kirti T Mohapatra H Khanna S Prakash VL Chopra (1995) ArticleTitleDiplotaxis catholica + Brassica juncea somatic hybrids: molecular and cytogenetic characterization Plant Cell Rep. 14 593–597
PB Kirti SB Narasimhulu S Prakash VL Chopra (1992) ArticleTitleSomatic hybridization between Brassica juncea and Moricandia arvensis by protoplast fusion Plant Cell Rep. 11 318–321
PB Kirti SB Narasimhulu S Pradash VL Chopra (1992) ArticleTitleProduction and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea Plant Cell Rep. 11 90–92
PB Kirti S Prakash K Gaikwad V Dinesh Kumar SR Bhat VL Chopra (1998) ArticleTitleChloroplast substitution overcomes leaf chlorosis in a Moricandia arvensis based cytoplasmic male sterile Brassica juncea Theor. Appl. Genet. 97 1179–1182
H Kisaka T Kameya (1994) ArticleTitleProduction of somatic hybrids between Daucus carota L. and Nicotiana tabacum Theor. Appl. Genet. 88 75–80
H Kisaka M Kisaka A Kanno T Kameya (1997) ArticleTitleProduction and analysis of plants that are somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.). Theor Appl. Genet. 94 221–226
H Kisaka M Kisaka A Kanno T Kameya (1998) ArticleTitleIntergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion Plant Cell Rep. 17 362–367
H Kisaka H Lee M Kisaka A Kanno K Kang T Kameya (1994) ArticleTitleProduction and analysis of asymmetric hybrid plants between monocotyledon (Oryza sativa L) and dicotyledon (Daucus carota L) Theor. Appl. Genet. 89 365–371
I Kishinami JM Widholm (1987) ArticleTitleAuxotrophic complementation in intergeneric hybrid cells obtained by electrical and dextran-induced protoplast fusion Plant Cell Physiol. 28 211–218
K Klimaszewska WA Keller (1988) ArticleTitleRegeneration and characterization of somatic hybrids between Brassica napus and Diplotaxis harra Plant Sci. 58 211–222
RS Kobayashi JR Stommel SL Sinden (1996) ArticleTitleSomatic hybridization between Solanum ochranthum and Lycopersicon esculentum Plant Cell Tiss. Organ Cult. 45 73–78
I Kostenyuk O Lubaretz N Borisyuk V Voronin J Stockigt Y Gleba (1991) ArticleTitleIsolation and characterization of intergeneric somatic hybrids in the Apocynaceae family Theor. Appl. Genet. 82 713–716
G Krumbiegel O Schieder (1979) ArticleTitleSelection of somatic hybrids after fusion of protoplast from Datura innoxia and Atropa belladonna Planta 145 371–375
M Kulawiec N Tagashira W Plader G Bartoszewski D Kuc R Sniezko S Malepszy (2003) ArticleTitleChromosome number variation in somatic hybrids between transgenic tomato (Lycopersicon esculentum) and Solanum lycopersicoides J. Appl. Genet. 44 431–437
A Kumar EC Cocking (1987) ArticleTitleProtoplast fusion: a novel approach to organelle genetics in higher plants Am. J. Bot. 74 1289–1303
S Kushnir E Babiychuk M Bannikova V Momot I Komarnisky N Cherep YY Gleba (1991) ArticleTitleNucleaocytoplasmic incompatibility in cybrid plants possessing an Atropa genome and a Nicotiana plastome Mol. Gen. Genet. 225 225–230
S Kushnir LR Shlumakov NJ Pogrebnyak S Berger YY Gleba (1987) ArticleTitleFunctional cybrid plants, possessing a Nicotiana genome and an Atropa plastome Mol. Gen. Genet. 209 159–163
M Leino R Teixeira M Landgren K Glimelius (2003) ArticleTitleBrassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations Theor. Appl. Genet. 106 1156–1163
M Leino S Thyselius M Landgren K Glimelius (2004) ArticleTitleArabidopsis thaliana chromosome III restored fertility in a cytoplasmic male-sterile Brassica napus line with A. thaliana mitochondrial DNA Theor. Appl. Genet. 109 272–279
CLC Lelivelt FA Krens (1992) ArticleTitleTransfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) into the Brassica napus L. gene pool through intergeneric somatic hybridization with Raphanus sativus L Theor. Appl. Genet. 83 887–894
CLC Lelivelt EHM Leunissen HJ Frederiks JPFG Helsper FA Krens (1993) ArticleTitleTransfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) from Sinapis alba L. (white mustard) to the Brassica napus L. gene pool by means of sexual and somatic hybridization Theor. Appl. Genet. 85 688–696
XH Li WB Li MJ Huang (1982) ArticleTitleSomatic hybrid plant from intergeneric fusion between tobacco tumor B653 and Petunia hybrida W43 and expression of LpDH Sci. China 25 611–619
Y Li KC Sink (1992) ArticleTitleCell type determines plastid transmission in tomato intergeneric somatic hybrids Curr. Genet. 22 167–171
YG Li J Tanner AC Delves PJ Larkin (1993) ArticleTitleAsymmetric somatic hybrid plants between Medicago sativa L (Alfalfa, lucerine) and Onobrychis viciifolia Scop (sainfoin) Theor. Appl. Genet. 87 455–463
LL Li GM Xia HM Chen (2001) ArticleTitleAsymmetric hybridization between wheat and millet Acta Phytophysiol. Sin. 27 455–460
JT Ling M Iwamasa (1994) ArticleTitleSomatic hybridization between Citrus reticulata and Citropsis gabunensis through electrofusion Plant Cell Rep. 13 493–497
B Liu DJ Liu (1995) ArticleTitleTransfer of partial genome of Avena nuda L. into Triticum aestivum L. by donor-recipient protoplast fusion Acta Biol. Exp. Sin. 28 95–101
B Liu ZL Liu XW Li (1999) ArticleTitleProduction of a highly asymmetric somatic hybrid between rice and Zizania latifolia (Griseb): evidence for inter-genomic exchange Theor. Appl. Genet. 98 1099–1103
B Liu WJ Tao ZH Zhang M Xing H Xie MY He S Hao (1995) ArticleTitleIntergeneric protoplast fusion between N. rustica L. and L. barbarum L. and hybrid shoot formation Acta Biol. Exp. Sin. 28 319–327
B Liu M Xing ZH Zhang MY He S Hao DF Gu R Zhao XK Wu (1995) ArticleTitleIntergeneric somatic hybrid plants of Nicotiana tabacum L. and Lycium barbarum L. by protoplast electro-fusion (in Chinese) Acta Bot. Sin. 37 259–266
JH Liu XX Deng (1999) ArticleTitleRegeneration of hybrid calluses via donor–recipient fusion between Microcitrus papuana and Citrus sinensis Plant Cell Tiss. Org. Cult. 59 81–87
JH Liu XX Deng (2000) ArticleTitleRegeneration of intergeneric hybrid embryoids via protoplast asymmetric fusion (in Chinese) Acta Hortic. Sin. 27 207–209
JH Liu XX Deng (2000) ArticleTitleRegeneration of intergeneric tetraploid somatic hybrids via electrofusion in Citrus (in Chinese) Sci. Agric. Sin. 33 98–100
JH Liu CG Hu XX Deng (1999) ArticleTitleRegeneration of diploid intergeneric somatic hybrid plants between Microcitrus and Citrus via electrofusion Acta Bot. Sin. 41 1177–1182
JH Liu CG Hu XX Deng (2000) ArticleTitleProduction of intergeneric somatic hybrid plants via protoplast electrofusion in Citrus (in Chinese) Acta Biol. Exp. Sin. 33 325–332
JH Liu CG Hu XX Deng (2002) ArticleTitleProduction of citrus intergeneric somatic hybrid plant from electricity-mediated protoplast fusion (in Chinese) Acta Hortic. Sin. 29 372–374
JH Liu XM Pang YJ Cheng HJ Meng XX Deng (2002) ArticleTitleMolecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and rough lemon Plant Cell Rep. 21 327–332
KB Liu YM Li KC Sink (1995) ArticleTitleAsymmetric somatic hybrid plants between an interspecific Lycopersicon hybrid and Solanum melongena Plant Cell Rep. 14 652–656
ES Louzada JW Grosser FG Gmitter SuffixJr (1993) ArticleTitleIntergeneric somatic hybridization of sexually incompatible Citrus sinensis and Atalantia ceylanica Plant Cell Rep. 12 687–690
PF McCabe LJ Dunbar A Guri KC Sink (1993) ArticleTitleT-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides Theor. Appl. Genet. 86 377–382
G Melchers Y Mohri K Watanabe S Wakabayashi K Harada (1992) ArticleTitleOne-step generation of cytoplasmic male sterility by fusion of mitochondria-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts Proc. Natl. Acad. Sci. USA 89 6832–6836
G Melchers MD Sacristan AA Holder (1978) ArticleTitleSomatic hybrid plants of potato and tomato regenerated from fused protoplasts Carlsberg Res. Commun. 43 203–218
JM Melzer MA O’Connell (1992) ArticleTitleEffect of radiation dose on the production of and the extent of asymmetry in tomato asymmetric somatic hybrids Theor. Appl. Genet. 83 337–344
M Miranda T Motomura F Ikeda T Ohgawara W Saito T Endo M Omura T Moriguchi (1997) ArticleTitleSomatic hybrids obtained by fusion between Poncirus trifoliata (2X) and Fortunella hindsii (4X) protoplasts Plant Cell Rep. 16 401–405
T Mohapatra PB Kirti VD Kumar S Prakash VL Chopra (1998) ArticleTitleRandom chloroplast segregation and mitochondrial genome recombination in somatic hybrid plants of Diplotaxis catholica + Brassica juncea Plant Cell Rep. 17 814–818
PP Moore KC Sink (1988) ArticleTitleMolecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric somatic hybrid plants Plant Mol. Biol. 11 139–145
CD Moreira CD Chase FG Gmitter SuffixJr JW Grosser (2000) ArticleTitleInheritance of organelle genomes in citrus somatic cybrids Mol. Breed. 6 401–405
A Morgan P Maliga (1987) ArticleTitleRapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids Mol. Genet. Gen. 209 240–246
T Motegi S Nou JM Zhou A Kanno T Kameya Y Hirata (2003) ArticleTitleObtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile) Euphytica 129 319–323
T Motomura T Hidaka T Akihama S Katagi M Berhow T Moriguchi M Omura (1997) ArticleTitleProtoplast fusion for production of hybrid plants between Citrus and its related genera J. Jpn. Soc. Hortic. Sci. 65 685–692
T Motomura T Hidaka T Moriguchi T Akihama M Omura (1995) ArticleTitleIntergeneric somatic hybrids between Citrus and Atalantia or Severinia by electrofusion and recombination of mitochondrial genomes Breed. Sci. 45 309–314
T Motomura T Moriguchi T Akihama T Hidaka M Omura (1996) ArticleTitleAnalysis of cytoplasmic genome in somatic hybrids between ‘Hazzara (Abohar)’ (Citrus reticulata Blanco) and Microcitrus australis (Planch.) Swingle J. Jpn. Soc. Hortic. Sci. 65 497–503
S Nakajo M Niizeki T Harada R Ishikawa K Saito (1994) ArticleTitleSomatic cell hybridization in rice (Oryza sativa L.) and birdsfoot trefoil (Lotus corniculatus L.). Breed Sci. 44 79–81
M Nakano Y Hoshino M Mii (1996) ArticleTitleIntergeneric somatic hybrid plantlets between Dianthus barbatus and Gypsophila paniculata obtained by electrofusion Theor. Appl. Genet. 92 170–172
M Nakano M Mii (1993) ArticleTitleCallus and root formation from an intergeneric somatic hybrid between Dianthus caryophyllus and Gypsophila paniculata Sci. Hortic. 53 13–19
SB Narashimhulu PB Kirti SR Bhatt S Prakash VL Chopra (1994) ArticleTitleIntergeneric protoplast fusion between Brassica carinata and Camelina sativa Plant Cell Rep. 13 657–660
B Navrátilová J Buzek J Siroky P Havranek (1997) ArticleTitleConstruction of intergeneric somatic hybrids between Brassica oleracea and Armoracia rusticana Biol. Plant. 39 531–541
M Niizeki M Tanaka S Akada A Hirai K Saito (1985) ArticleTitleCallus formation of somatic hybrid of rice and soybean and characteristics of the hybrid callus Jpn. J. Genet. 60 81–92
HT Nivison MR Hanson (1989) ArticleTitleIdentification of a mitochondrial protein associated with cytoplasmic male sterility in petunia Plant Cell 1 1121–1130
T Nothnagel H Budahn P Straka O Schrader (1997) ArticleTitleSuccessful backcrosses of somatic hybrids between Sinapis alba and Brassica oleracea with the Brassica oleracea parent Plant Breed. 116 89–97
SJ Ochatt EM Patat-Ochatt EL Rech MR Davey JB Power (1989) ArticleTitleSomatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (Pyrus communis var Pyraster L.) and Colt cherry (Prunus avium × pseudocerasus). Theor. Appl. Genet. 78 35–41
MA O’Connell MR Hanson (1986) ArticleTitleRegeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii Theor. Appl. Genet. 72 59–65
T Ohgawara S Kobayashi S Ishii K Yoshinaga I Oiyama (1991) ArticleTitleFertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis × Poncirus trifoliata) Theor. Appl. Genet. 81 141–143
T Ohgawara S Kobayashi E Ohgawara H Uchimiya S Ishii (1985) ArticleTitleSomatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata Theor. Appl. Genet. 71 1–4
P Ollitrault D Dambier F Luro (1996) ArticleTitleSomatic hybridization in Citrus: some new hybrids and alloplasmic plants Proc. Int. Soc. Citricult. 2 907–912
CM O’Neill T Murata CL Morgan RJ Mathias (1996) ArticleTitleExpression of the C3–C4 intermediate character in somatic hybrids between Brassica napus and the C3–C4 species Moricandia arvensis Theor. Appl. Genet. 93 1234–1241
W Orczyk J Przetakiewicz A Nadolska-Orczyk (2003) ArticleTitleSomatic hybrids of Solanum tuberosum-application to genetics and breeding Plant Cell Tiss. Organ Cult. 74 1–13
G Pelletier C Primard F Vedel P Chetrit R Remy (1983) ArticleTitleIntergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion (Brassica napus, Raphanus sativus, Brassica campestris) Mol. Gen. Genet. 191 244–250
D Pental JD Hamill EC Cocking (1984) ArticleTitleSomatic hybridization using a double mutant of Nicotiana tabacum Heredity 53 79–83
D Pental JD Hamill A Pirri EC Cocking (1986) ArticleTitleSomatic hybridization of Nicotiana tabacum and Petunia hybrida Recovery of plants with P. hybrida nuclear genome and N. tabacum chloroplast genome. Mol. Gen. Genet. 202 342–347
A Perl D Aviv E Galun (1991) ArticleTitleProtoplast fusion mediated transfer of oligomycin resistance from Nicotiana sylvestris to Solanum tuberosum by intergeneric cybridization Mol. Gen. Genet. 225 11–16
I Potrykus JF Jia GB Lazar M Saul (1984) ArticleTitleHyoscyamus muticus × Nicotiana tabacum fusion hybrids selected via auxotroph complementation Plant Cell Rep. 3 68–71
S Prakash PB Kirti SR Bhat K Gaikwad VD Kumar VL Chopra (1998) ArticleTitleA Moricandia arvensis-based cytoplasmic male sterility and fertility restoration system in Brassica juncea Theor. Appl. Genet. 97 488–492
C Primard F Vedel C Mathieu G Pelletier AM Chevre (1988) ArticleTitleInterspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapsis alba L.). Theor Appl. Genet. 75 546–552
C Rambaud J Dubois J Vasseur (1993) ArticleTitleMale-sterile chicory cybrids obtained by intergeneric protoplast fusion Theor. Appl. Genet. 87 347–352
KS Ramulu P Dijkhuis I Famelaer T Cardi HA Verhoeven (1994) ArticleTitleCremart: a new chemical for efficient induction of micronuclei in cells and protoplasts for partial genome transfer Plant Cell Rep. 13 687–691
KS Ramulu P Dijkhuis E Rutgers J Blaas WHJ Verbeek HA Verhoeven CM Colijn-Hooymans (1995) ArticleTitleMicroprotoplast fusion technique: a new tool for gene transfer between sexually-incongruent plant species Euphytica 85 255–268
KS Ramulu P Dijkhuis E Rutgers J Blaas FA Krens JJM Dons CM Coliin-Hooymans HA Verhoeven (1996) ArticleTitleMicroprotoplast-mediated transfer of single specific chromosomes between sexually incompatible plants Genome 39 921–993
KS Ramulu P Dijkhuis E Rutgers J Blaas FA Krens WHJ Verbeek CM Coliin-Hooymans HA Verhoeven (1996) ArticleTitleIntergeneric transfer of a partial genome and direct production of monosomaic addition plants by microprotoplast fusion Theor. Appl. Genet. 92 316–325
E Rutgers KS Ramulu P Dijkhuis J Blaas FA Krens HA Verhoeven (1997) ArticleTitleIdentification and molecular analysis of transgenic potato chromosomes transferred to tomato through microprotoplast fusion Theor. Appl. Genet 94 1053–1059
T Sakai J Imamura (1990) ArticleTitleIntergeneric transfer of cytoplasmic male sterility between Raphanus sativus (CMS line) and Brassica napus through cytoplast–protoplast fusion, Theor Appl. Genet. 80 421–427
T Sakai J Imamura (1992) ArticleTitleAlteration of mitochondrial genomes containing atpA genes in the sexual progeny of cybrids between Raphanus sativus cms line and Brassica napus cv Westar. Theor. Appl. Genet. 84 923–929
T Sakai HJ Liu M Iwabuchi J Kohno-Murase J Imamura (1996) ArticleTitleIntroduction of a gene from fertility restored radish (Raphanus sativus) into Brassica napus by fusion of X-irradiated protoplasts from a radish restorer line and iodacetoamide-treated protoplasts from a cytoplasmic male-sterile cybrid of B. napus Theor. Appl. Genet. 93 373–379
K Sakomoto T Taguchi (1991) ArticleTitleRegeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum Theor. Appl. Genet. 81 509–513
VM Samoylov S Izhar KC Sink (1996) ArticleTitleDonor chromosome elimination and organelle composition of asymmetric somatic hybrid plants between an interspecific tomato hybrid and eggplant Theor. Appl. Genet. 93 268–274
VM Samoylov K Sink (1996) ArticleTitleThe role of irradiation dose and DNA content of somatic hybrid calli in producing asymmetric plants between an interspecific tomato hybrid and eggplant Theor. Appl. Genet. 92 850–857
B Schiller RG Herrmann G Melchers (1982) ArticleTitleRestriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids Mol. Gen. Genet. 186 453–459
H Schnabl TL Mahaworasilpa HGL Coster KellerA Von (1999) ArticleTitleProduction of hybrid cells from single protoplasts of sunflower hypocotyl and broad bean guard cells by electrical fusion Plant Cell Tiss. Org. Cult. 55 59–62
PS Schnable RP Wise (1998) ArticleTitleThe molecular basis of cytoplasmic male sterility and fertility restoration Trend Plant Sci. 3 175–180
HCH Schoenmakers der Meulen-MuisersJJM van M Koornneef (1994) ArticleTitleAsymmetric fusion between protoplasts of tomato (Lycoperiscon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation Mol. Gen. Genet. 242 313–320
HCH Schoenmakers AMA Wolters de Haan A AK Saiedi M Koorneeff (1994) ArticleTitleAsymmetric somatic hybridization between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato (Solanum tuberosum L.): a quantitative analysis Theor. Appl. Genet. 87 713–720
HCH Schoenmakers AMA Wolters EM Nobel CMJ Klein Particlede M Koornneef (1993) ArticleTitleAllotriploid somatic hybrids of diploid tomato (Lycopersicon esculentum Mill.) and monoploid potato (Solanum tuberosum L.). Theor Appl. Genet. 87 328–336
G Schweizer M Ganal H Ninnemann V Hemleben (1988) ArticleTitleSpecies-specific DNA sequence for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule Theor. Appl. Genet. 75 679–684
JF Shepard D Bidney T Barsby R Kemble (1983) ArticleTitleGenetic transfer in protoplast through interspecific protoplast fusion Sci. 219 683–688
I Sherraf S Tizroutine MH Chaput M Allot I Mussio D Sihachakr L Rossignol G Ducreux (1994) ArticleTitleProduction and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii Plant Cell Tiss. Org. Cult. 37 137–144
T Shikanai H Kaneko S Nakata K Harada K Watanabe (1998) ArticleTitleMitochondrial genome structure of a cytoplasmic hybrid between tomato and wild potato Plant Cell Rep. 17 832–836
S Shinozaki K Frina T Hidaka M Omura (1992) ArticleTitlePlantlet formation of somatic hybrids of sweet orange (Citrus sinensis) and its wild relative, orange jessamine (Murraya paniculata) by electrically-induced protoplast fusion Jpn. J. Breed. 42 287–295
J Siemens MD Sacristan (1995) ArticleTitleProduction and characterization of somatic hybrids between Arabidopsis thaliana and Brassica nigra Plant Sci. 111 95–106
MA Sigareva ED Earle (1999) ArticleTitleCamalexin induction in intertribal somatic hybrids between Camelina sativa and rapid-cycling Brassica oleracea Theor. Appl. Genet. 98 164–170
MA Sigareva ED Earle (1999) ArticleTitleRegeneration of plants from protoplasts of Capsella bursa-pastoris and somatic hybridization with rapid cycling Brassica oleracea Plant Cell Rep. 18 412–417
SR Sikdar G Chatterjee S Das SK Sen (1990) ArticleTitle‘Erussica’, the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa Lam and Brassica juncea (L.) Czern Theor. Appl. Genet. 79 561–567
M Skarzhinskaya J Fahleson K Glimelius A Mouras (1998) ArticleTitleGenome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization Genome 41 691–701
M Skarzhinskaya M Landgren K Glimelius (1996) ArticleTitleProduction of intertribal somatic hybrids between Brassica napus L. and Lesquerella gendleri (Gray) Wats Theor. Appl. Genet. 93 1242–1250
MV Smith A Pay D Dudits (1989) ArticleTitleAnalysis of chloroplast and mitochondrial DNAs in asymmetric hybrids between tobacco and carrot Theor. Appl. Genet. 77 641–644
DA Somers KR Narayanan A Kleinhofs S Copper-Bland EC Cocking (1986) ArticleTitleImmunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion Mol. Gen. Genet. 204 296–301
XQ Song GM Xia AF Zhou XZ Bao HM Chen (1999) ArticleTitleHybrid plant regeneration from interfamiliar somatic hybridization between grapevine (Vitis vinifera L.) and thorowax (Bupleurum scorzonerifolium Willd) Chin. Sci. Bull. 44 1878–1882
G Spangenberg MP Valles ZY Wang P Montavon J Nagel I Potrykus (1994) ArticleTitleAsymmetric somatic hybridization between tall fescue (Festuca arundinaceae Schreb) and irradiated Italian ryegrass (Lolium multiflorum Lam) protoplast Theor. Appl. Genet. 88 509–519
G Spangenberg ZY Wang G Legris P Montavon T Takamizo R Perezvicente MP Valles J Nagel I Potrykus (1995) ArticleTitleIntergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium Euphytica 85 235–245
E Sundberg K Glimelius (1991) ArticleTitleEffects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae Theor. Appl. Genet. 83 81–88
B Szarka L Gonter M Mlonar-lang S Morocz D Dudits (2002) ArticleTitleMixing of maize and wheat genomic DNA by somatic hybridization in regenerated sterile maize plants Theor. Appl. Genet. 105 1–7
Z Tabaeizadeh RJ Ferl IK Vasil (1986) ArticleTitleSomatic hybridization in the Gramineae: Saccharum officinarum L. (Sugarcane) and Pennisetum americanum (L.) K. Schum Proc. Natl. Acad. Sci. USA 83 5616–5619
K Takami A Matsumara M Yahata T Imayama H Kunitake H Komatsu (2004) ArticleTitleProduction of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck) Plant Cell Rep. 23 39–45
T Takamizo G Spangenberg KI Suginobu I Potrykus (1991) ArticleTitleIntergeneric somatic hybridization in Gramineae: somatic hybrid plants between tall fescue (Festuca arundinacea Schreb.) and Italian ryegrass (Lolium multiflorum Lam.). Mol Gen. Genet. 231 1–6
R Takayanagi T Hidaka M Omura (1992) ArticleTitleRegeneration of intergeneric somatic hybrids by electrical fusion between citrus and its wild relatives: Mexican lime (Citrus aurantifolia) and java feroniella (Feroniella lucida) or tabog (Swinglea glutinosa) J. Jpn. Soc. Hortic. Sci. 60 799–804
MJ Tempelaar JL Drenth-Diephuis TAWM Saar E Jacobsen (1991) ArticleTitleSpontaneous and induced loss of chromosomes in slow-growing somatic hybrid callus of Solanum tuberosum and Nicotiana plumbaginifolia Euphytica 56 287–296
R Terada J Kyozuka S Nishibayashi K Shimamoto (1987) ArticleTitlePlantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing) Mol. Gen. Genet. 210 39–43
ND Thanh P Medgyesy (1989) ArticleTitleLimited chloroplast gene transfer via recombination overcomes plastome-genome incompatibility between N. tabacum and S. tuberosum Plant Mol. Biol. 12 87–93
ND Thanh A Pay MA Smith P Medgyesy L Marton (1988) ArticleTitleIntertribal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata Mol. Gen. Genet. 213 186–190
S Toki T Kameya T Abe (1990) ArticleTitleProduction of triple mutant, chlorophyll-deficient, streptomycin-and kanamycin-resistant Nicotiana tabacum, and its use in intergeneric somatic hybrid formation with Solanum melongena Theor. Appl. Genet. 80 588–592
K Toriyama K Hinata T Kameya (1987) ArticleTitleProduction of somatic hybrid plants, ‘Brassicomoricandia’, through protoplast fusion between Moricandia arvensis and Brassica oleracea Plant Sci. 48 123–128
K Toriyama T Kameya K Hinata (1987) ArticleTitleSelection of a universal hybridizer in Sinapsis turgida Del and regeneration of plantlets from somatic hybrids with Brassica species Planta 170 308–313
Y Toriyama T Yanagino K Razmjoo R Ishii K Hinata (1988) ArticleTitleChloroplast DNA and CO 2 compensation point of somatic hybrid plants between Brassica oleracea and Moricandia arvensis Jpn. J. Genet. 63 543–547
HN Trick GW Bates (1996) ArticleTitleBromodeoxyuridine combine with UV light and gamma irradiation promotes the production of asymmetric somatic hybrid calli Plant Cell Rep. 15 986–990
H Trick A Zelcer GW Bates (1994) ArticleTitleChromosome elimination in asymmetric somatic hybrids: effect of gamma dose and time in culture Theor. Appl. Genet. 88 965–972
A Vardi P Arzee-Gonen A Frydman-Shani S Bleichman E Galun (1989) ArticleTitleProtoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees Theor. Appl. Genet. 78 741–747
A Vardi A Breiman E Galun (1987) ArticleTitleCitrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles Theor. Appl. Genet. 75 51–58
S Varotto E Nenz M Lucchin P Parrini (2001) ArticleTitleProduction of asymmetric somatic hybrid plants between Cichorium intybus L. and Helianthus annuus L Theor. Appl. Genet. 102 950–956
V Vasil RJ Perl JK Vasil (1988) ArticleTitleSomatic hybridization in the Gramineae: Triticum monococcum + Pennisetum americanum (L.) K. Schum (pearl millet). J. Plant Physiol. 123 160–163
MIu Vasilenko IK Komarnitskii LA Sakhno IuIu Gleba NV Kuchuk (2003) ArticleTitleObtaining and analysis of intergeneric somatic hybrids between Brassica napus and “albino” line of Orychophragmus violaceus Tsitol. Genet. 37 3–10
A Vazquez-Thello LJ Yang M Hidaka T Uozumi (1996) ArticleTitleInherited chilling tolerance in somatic hybrids of transgenic Hibiscus rosa-sinensis × transgenic Lavatera thuringiaca selected by double-antibiotic resistance Plant Cell Rep. 15 506–511
M Vlahova S Hinnisdaels F Frulleux M Claeys A Atanassov M Jacobs (1997) ArticleTitleUV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginifolia and Lycopersicon esculentum Theor. Appl. Genet. 94 184–191
SE Vries Particlede MA Ferwerda AEHM Loonen LP Pijnacker WJ Feenstra (1987) ArticleTitleChromosomes in somatic hybrids between Nicotiana plumbaginifolia and a monoploid potato Theor. Appl. Genet. 75 170–176
XS Wan FD Wang GY Wang XF Ye ZA Xia (1988) ArticleTitleRegeneration of somatic hybrid plants of tobacco (Nicotiana tabacum L.) and black nightshade (Solanum nigrum L.). Acta Exp Biol. Sin. 21 245–248
TB Wang M Niizeki T Harada R Ishikawa YQ Qian K Saito (1993) ArticleTitleEstablishment of somatic hybrid cell lines between Zea mays L. (maize) and Triticum sect. trititrigia MacKey (trititrigia) Theor. Appl. Genet. 86 371–376
YP Wang RJ Snowdon E Rudloff P Wehling W Friedt K Sonntag (2004) ArticleTitleCytogenetic characterization and fae1 gene variation in progenies from asymmetric somatic hybrids between Brassica napus and Crambe abyssinica Genome 47 724–731
YP Wang K Sonntag E Rudloff (2003) ArticleTitleDevelopment of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica Theor. Appl. Genet. 106 1147–1155
FD Wang XS Wan XF Ye ZA Xia (1989) ArticleTitleSomatic hybrids obtained from fusion of carrot and celery protoplasts (in Chinese) Plant Physiol. Commun. 1 26–29
LR Wetter KN Kao (1980) ArticleTitleChromosome and isoenzyme studies on cells derived from protoplast fusion of Nicotiana glauca with Glycine max–Nicotiana glauca cell hybrids Theor. Appl. Genet. 57 273–276
AMA Wolters EO Jacobsen M Connell G Bonnema KS Ramula H Jong K Schoenmakers J Wijbrandi M Koomneef (1994) ArticleTitleSomatic hybridization as a tool for tomato breeding Euphytica 79 265–277
AMA Wolters M Koornneef LJW Gilissen (1993) ArticleTitleThe chloroplast and mitochondrial DNA type are correlated with the nuclear composition of somatic hybrid calli of Solanum tuberosum and Nicotiana plumbaginifolia Curr. Genet. 24 260–267
AMA Wolters HCH Schoenmakers M Koornneef (1995) ArticleTitleChloroplast and mitochondrial DNA composition of triploid and tetraploid somatic hybrids between Lycopersicon esculentum and Solanum tuberosum Theor. Appl. Genet. 90 285–293
AMA Wolters HCH Schoenmakers JJM Meuen-Muiisers E Knaap Particlevan der FHM Derks M Koornneef A Zelcer (1991) ArticleTitleLimited DNA elimination from irradiated potato parent in fusion products of albido Lycopersicon esculentum Theor. Appl. Genet. 83 225–232
AMA Wolters AC Vergunst F Werff Particlevan der M Koornneef (1993) ArticleTitleAnalysis of nuclear and organellar DNA of somatic hybrid calli and plants between Lycopersicon spp. and Nicotiana spp Mol. Gen. Genet. 241 707–718
GM Xia HM Chen (1996) ArticleTitlePlant regeneration from intergeneric somatic hybridization between Triticum aestivum L and Leymus chinensis (Trin) Tzvel Plant Sci. 120 197–203
GM Xia SY Chen FN Xiang TY Quan HM Chen (2001) ArticleTitleStudy on salt-tolerance of F3–F5 hybrids originated from somatic hybridization between wheat and Agropyron elongatum Shandong Agric. Sci. 6 12–14
GM Xia ZY Li SL Wang FN Xiang JY Liu PD Chen DJ Liu (1998) ArticleTitleAsymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa Plant Sci. 137 217–223
GM Xia H Wang HM Chen (1996) ArticleTitlePlant regeneration from intergeneric asymmetric hybridization between wheat (Triticum aestivum L.) and Russian wildrye (Psathyrostachys juncea (Fisch) nevski) and Couch grass (Agropyron elongatum (Host) Nevski) Chin. Sci. Bull. 41 1382–1386
GM Xia FN Xiang AF Zhou H Wang HM Chen (2003) ArticleTitleAsymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi Theor. Appl. Genet. 107 299–305
GM Xia FN Xiang AF Zhou H Wang SX He HM Chen (1999) ArticleTitleFertile hybrid plant regeneration from somatic hybridization between Triticum aestivum and Agropyron elongatum Acta Bot. Sin. 41 349–352
FN Xiang GM Xia HM Chen (2003) ArticleTitleAsymmetric somatic hybridization between wheat (Triticum aestivum) and Avena sativa L Sci. China (Series C) 46 243–252
FN Xiang GM Xia HM Chen (2003) ArticleTitleEffect of UV dosage on somatic hybridization between common wheat (Triticum aestivum L.) and Avena sativa L Plant Sci. 164 697–707
FN Xiang GM Xia AF Zhou HM Chen Y Huang XL Zhai (1999) ArticleTitleAsymmetric somatic hybridization between wheat (Triticum aestivum) and Bromus inermus Acta Bot. Sin. 41 458–462
FN Xiang GM Xia DY Zhi J Wang H Nie HM Chen (2004) ArticleTitleRegeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica Genome 47 680–688
H Xie B Liu ZH Zhang ZJ Bao WJ Tao MY He (1996) ArticleTitleIntergeneric protoplast fusion between Nicotiana undulata L. and Lycium barbaum L. and regeneration of hybrid plants (in Chinese) Acta Agro. Sin. 22 1–5
HW Xin JS Sun QS Yan XQ Zhang (1997) ArticleTitlePlant regeneration from asymmetric somatic hybrids of Oryza sativa and Panicum maximum Acta Bot. Sin. 39 717–724
HQ Xing GM Xia HM Chen (2001) ArticleTitlePreliminary study on hybrid chromosome composition and relationship in symmetric hybridization between Triticum aestivum and intergeneric grasses (in Chinese) Bull. Bot. Res. 21 74–78
XY Xu JH Liu XX Deng (2004) ArticleTitleProduction and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium) Euphytica 136 115–123
CH Xu GM Xia DY Zhi FN Xiang HM Chen (2003) ArticleTitleIntegration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization Plant Sci. 165 1001–1008
H Yamagishi M Landgren J Forsberg K Glimelius (2002) ArticleTitleProduction of asymmetric hybrids between Arabidopsis thaliana and Brassica napus utilizing an efficient protoplast culture system Theor. Appl. Genet. 104 959–964
J Yamaguchi T Shiga (1993) ArticleTitleCharacteristics of regenerated plants via electrofusion between melon (Cucumis melo) and pumpkin (interspecific hybrid, Cucurbita maxima × C. moschata) Jpn. J. Breed. 43 173–182
H Yamanaka Y Kuginuki T Kanno T Nishio (1992) ArticleTitleEfficient production of somatic hybrids between Raphanus sativus and Brassica oleracea Jpn. J. Breed. 42 329–339
Z Yan Z Tian B Huang R Huang J Meng (1999) ArticleTitleProduction of somatic hybrids between Brassica oleracea and the C3–C4 intermediate species Moricandia nitens Theor. Appl. Genet. 99 1281–1286
J Ye RM Hauptmann AG Smith JM Widholm (1987) ArticleTitleSelection of a Nicotiana plumbaginifolia universal hybridizer and its use in intergeneric somatic hybrid form Mol. Gen. Genet. 208 474–480
AI Yemets OP Kundel’chuk AP Smertenko VG Solodushko VA Rudas YY Gleba YB Blume (2000) ArticleTitleTransfer of amiprophosmethyl resistance from a Nicotiana plumbaginifolia mutant by somatic hybridization Theor. Appl. Genet. 100 847–857
EG Young MR Hanson (1987) ArticleTitleA fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated Cell 50 41–49
W Yue GM Xia DY Zhi HM Chen (2001) ArticleTitleTransfer of salt tolerance from Aeleuropus littoralis Sinensis to wheat (Triticum aestivum) via asymmetric somatic hybridization Plant Sci. 161 259–263
XG Zhang PY Liu (1998) ArticleTitleProtoplast fusion between Cucumis sativus, Cucurbita moschata and Cucurbita ficifolia (in Chinese) J. Southwest Agric. Univ. 20 293–297
AF Zhou XL Chen GM Xia HM Chen (2002) ArticleTitleUV-fusion between protoplasts of common wheat and Haynaldia villosa J. Plant Physiol. Mol. Biol. 28 305–310
AF Zhou GM Xia HM Chen (1996) ArticleTitleAsymmetric somatic hybridization between Triticum aestivum and Haynaldia villosa Schur Sci. China (Series C) 39 617–626
AF Zhou GM Xia HM Chen (2001) ArticleTitleComparative study of symmetric and asymmetric somatic hybridization between common wheat and Haynaldia villosa Sci. China (Series C) 44 294–304
AF Zhou GM Xia XL Chen HM Chen (2002) ArticleTitleProduction of somatic hybrid plants between two types of wheat protoplasts and the protoplasts of Haynaldia villosa Acta Bot. Sin. 44 1004–1008
A Zhou G Xia X Zhang H Chen H Hu (2001) ArticleTitleAnalysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur Mol. Genet. Gen. 265 387–393
MK Zubko EI Zubko K Adler B Grimm YY Gleba (2003) ArticleTitleNew CMS phenotypes in cybrids Nicotiana tabacum L. (+ Hyoscyamus niger L.). Ann Bot. 92 281–288
MK Zubko EI Zubko YY Gleba (2002) ArticleTitleSelf-fertile cybrids Nicotiana tabacum (+ Hyoscyamus aureus) with a nucleo–plastome incompatibility Theor. Appl. Genet. 105 822–828
MK Zubko EI Zubko YV Patskovsky OA Khvedynich J Fisahn YY Gleba O Schieder (1996) ArticleTitleNovel ‘homeotic’ CMS patterns generated in Nicotiana via cybridization with Hyoscyamus and Scopolia J. Exp. Bot. 47 1101–1110
MK Zubko EI Zubko AV Ruban K Adler HP Mock S Misera YY Gleba B Grimm (2001) ArticleTitleExtensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+Hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility Plant J. 25 627–639
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Liu, J., Xu, X. & Deng, X. Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tiss Organ Cult 82, 19–44 (2005). https://doi.org/10.1007/s11240-004-6015-0
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DOI: https://doi.org/10.1007/s11240-004-6015-0