Abstract
Tissue-cultured plantlets of three sugarcane (Saccharum spp.) cultivars having a known field smut reaction were screened for susceptibility to Ustilago scitaminea H&P Sydow. Plantlets were inoculated with 0.5 μ l of a suspension of equally mixed quantities of plus and minus mating type sporidia of U. scitaminea at concentrations ranging from 1 × 101 to 1 × 106 cells. Fungal sori (whips) were produced in cultivar N12 (intermediate) 6 weeks following inoculation with 1 × 105 mixed sporidia and thereafter in cultivar NCo310 (susceptible) but not in cultivar N19 (resistant). Sori bearing teliospores were produced up to 3 months following inoculation and incubation at 26 °C. No sori were produced at mixed sporidial concentrations lower than 1 × 105cells. The in vitro soral production in cultivars N19, N12 and NCo310 was 0, 27.5 and 47.5% respectively. Plantlets inoculated with 1 × 105sporidia of only one mating-type did not produce sori in any of the three cultivars tested. Blind scoring of an unknown sugarcane cultivar by this method corresponded exactly with its field smut rating.
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References
HH Albert S Schenck (1996) ArticleTitlePCR amplification from a homolog of the bE mating-type gene as a sensitive assay for the presence of Ustilago scitaminea DNA Plant Dis. 80 1189–1192
KC Alexander KV Srinivasan (1966) ArticleTitleSexuality in Ustilago scitaminea Syd Curr. Sci. 35 603–604
RA Bailey GR Bechet (1982) ArticleTitleProgress in screening for resistance to sugarcane diseases in South Africa Proc. S. Afr. Sugar Technol. Assoc. 56 143–149
F Baunett I Herskowitz (1989) ArticleTitleDifferent a alleles of Ustilago maydis are necessary for the maintenance of filamentous growth but not for meiosis Proc. Nat. Acad. Sci. USA 86 5878–5882
KR Bock (1964) ArticleTitleStudies on sugar-cane smut (Ustilago scitaminea) in Kenya Trans. Br. Mycol. Soc. 47 403–417
DM Burner MP Grisham BL Legendre (1993) ArticleTitleResistance of sugarcane relatives injected with Ustilago scitaminea Plant Dis. 77 1221–1223
A Elsen R Stoffelen NT Tuyet H Baimey H Dupré de Boulois D De Waele (2002) ArticleTitleIn vitro screening for resistance to Radopholus smiles in Musa spp Plant Sci. 163 407–416
L Fereol (1984) ArticleTitleInnoculation of in vitro cultivated sugarcane with smut (Ustilago scitaminea) Can. J. Bot. 62 2043–2046
SA Ferreira JC Comstock (1989) Smut C Ricaud BT Egan AG Gillaspie SuffixJr CG Hughes (Eds) Diseases of Sugarcane, Major Diseases Elsevier New York 211–229
SA Ferreira JC Comstock KK Wu (1980) ArticleTitleEvaluating sugarcane for smut resistance Proc. Int. Soc. Sugar Cane Technol. 17 1463–1476
MMM Fitch PH Moore (1993) ArticleTitleLong-term culture of embryonic sugarcane callus Plant Cell Tissue Organ Cult. 32 335–343
E Hector MM Hernandez R Rodriguez (2000) ArticleTitleIn vitro tillering in the presence of U scitaminea filtrates as a criterion for smut resistance of sugarcane varieties. Cultivos Tropicales 21 33–35
DJ Heinz M Krishnamurti LG Nickell A Maretzki (1977) Cell tissue and organ culture in sugarcane improvement J. Reinert YPS Bajaj (Eds) Applied and Fundamental Aspects of Plant Cell Tissue, and Organ Culture Springer-Verlag Berlin 1–17
R Holliday (1974) Ustilago maydis RC King (Eds) Handbook of Genetics, Vol. I. Plenum Press New York 575–595
PB Hutchinson (1969) ArticleTitleA note on disease resistance ratings of sugarcane varieties Proc. Int. Soc. Sugar Cane Technol. 13 1087–1089
GL James (1969) ArticleTitleSmut susceptibility testing of sugarcane in Rhodesia Proc. S. Afr. Sugar Technol. Assoc. 43 85–92
S Kresovich RE McGee HL Drawe JL Rivera (1986) ArticleTitleVariability of agronomic characters in populations of tissue culture-derived and vegetatively-propagated sugarcane Proc. Int. Soc. Sugar Cane Technol. 19 528–532
HL Lloyd G Naidoo (1983) ArticleTitleChemical assay potentially suitable for determination of smut resistance of sugarcane cultivars Plant Dis. 67 1103–1105
HL Lloyd M Pillay (1980) ArticleTitleThe development of an improved method for evaluating sugarcane for resistance to smut Proc. S. Afr. Sugar Technol. Assoc. 54 168–172
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15 473–497
JE Parlevliet (1979) ArticleTitleComponents of resistance that reduce the rate of epidemic development Ann. Rev. Phytopath. 17 203–222
L Perez F Mauri (1983) ArticleTitleUstilago scitaminea Sydow in Cuba: biology, physiology and varietal reaction Proc. Int. Soc. Sugar Cane Technol. 18 778–795
J-P Péros P Chagvardieff (1983) ArticleTitleMise en evidence in vitro d’ effects toxiques entre Ustilago scitaminea Syd. Et des cals de canne a sucre; influence de deux facteurs de croissance sur les phenomenes observes Agronomie 3 629–634
J-P Péros P Chagvardieff (1987) ArticleTitleToxic effects of Ustilago scitaminea on sugarcane callus. II. Culture filtrate action and comparison with the effects of U. maydis and kinetin. J. Plant Dis. Prot. 94 301–307
C Ricaud (1981) ArticleTitleProposals for improving the use of ISSCT disease resistance ratings Sugarcane Pathol. Newsl. 26 27–40
S Schenck (1998) ArticleTitleEvaluation of a PCR amplification method for detection of systemic smut infections in sugarcane Sugar Cane 6 2–5
OK Sinha K Singh SR Misra (1982) ArticleTitleStain technique for detection of smut hyphae in nodal buds of sugarcane Plant Dis. 66 932–933
SJ Snyman GM Meyer DL Carson FC Botha (1996) ArticleTitleEstablishment of embryogenic callus and transient gene expression in selected sugarcane varieties S. Afr. J. Bot. 62 151–154
PWJ Taylor S Dukic (1993) ArticleTitleDevelopment of an in vitro culture technique for conservation of Saccharum spp. hybrid germplasm. Plant Cell Tissue Organ Cult. 34 217–222
PWJ Taylor H-L Ko SW Adkins C Rathus RG Birch (1992) ArticleTitleEstablishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell Tissue Organ Cult. 28 69–78
JM Waller (1969) ArticleTitleSugarcane smut (Ustilago scitaminea) in Kenya I. Epidemiology. Trans. Br. Mycol. Soc. 52 139–151
KK Wu DJ Heinz HK Meyer (1983) ArticleTitleHeritability of sugarcane smut resistance and correlation between smut grade and yield components Crop Sci. 23 54–56
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Singh, N., Somai, B.M. & Pillay, D. In vitro screening of sugarcane to evaluate smut susceptibility. Plant Cell Tiss Organ Cult 80, 259–266 (2005). https://doi.org/10.1007/s11240-004-1017-5
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DOI: https://doi.org/10.1007/s11240-004-1017-5