Abstract
Reliable large-scale maize production is an essential component of global food security; however, sustained efforts are needed to ensure optimized resilience under diverse crop stress conditions. Climate changes are expected to increase the frequency and intensity of both abiotic and biotic stress. Protective phytochemicals play an important role in both abiotic stress resilience and resistance to biotic challenges, yet the concentration and composition of these phytochemicals are also dependent on climate variables. We review the research on the effects of climate change associated abiotic stresses on three classes of maize defense metabolites, including benzoxazinoids, volatile organic compounds, and terpenoid phytoalexins. Despite significant knowledge gaps that still exist, it is evident that climate change will influence maize phytochemicals associated with resilient productivity. While broad generalizations are not yet possible, climate induced changes in phytochemicals are context specific and dependent upon developmental stage and tissue type. Under conditions of drought, maize modulates different classes of defense phytochemicals to protect the above-and belowground tissues. Aboveground the benzoxazinoid defenses are stimulated, but belowground terpenoid phytoalexins are predominantly deployed. Changes in the allocation or distribution of the different classes of defense metabolites or signaling molecules have the potential to further shape the biodiversity and abundance of pests within the maize agroecosystem. A better understanding of the underlying genetics, biosynthetic pathways, regulation and precise biological roles of maize phytochemicals modulated by arrays of climatic conditions will be required to ensure optimal plant resilience and productivity in the face of combined biotic and abiotic stresses.
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Introduction
Maize (Zea mays) is the most produced grain crop on earth. As an integral component of the world’s food supply, maize supports an exponentially growing human population either directly through consumption or indirectly through livestock feed. As an additional demand, there is also increasing use of maize for the production of bio-ethanol as a renewable source of energy and alternative to fossil fuels. To meet growing demands, the U.S. planted roughly 400,000 km2 of maize in 2012 which was the largest planting in nearly eight decades (Barton and Clark 2014). However, aberrant weather conditions consistent with climate change reduced the yield to the smallest crop harvest per unit area planted in over 20 years. The devastating effects of extreme heat and drought were exacerbated by the outbreak of corn rootworm (Petzold-Maxwell et al. 2013; Zukoff et al. 2016), and increased incidence of fungal infection resulting in mycotoxin contamination of the surviving grain (Bienkowski 2012; Schultz 2012; Smith and Mitchell 2012).
Extreme weather events and the negative impacts of abiotic stress on crop yields are increasing in frequency and intensity (Hatfield et al. 2011). With the continuous burning of fossil fuels and deforestation, the atmospheric carbon dioxide concentration ([CO2]) has risen from a pre-industrial concentration of 280 µmol CO2 mol−1 air to a current concentration of 400 µmol CO2 mol−1 air (1 × [CO2]) and is projected to reach 800 µmol CO2 mol−1 air (2 × [CO2]) before the end of this century (Solomon et al. 2007; Karl et al. 2009). Changes in the composition of atmospheric gases, such as CO2, are largely responsible for the greenhouse effect, leading to warmer temperatures and more severe precipitation events. While rising [CO2] benefits the photosynthetic activity of C3 plants, only relatively modest increases in C4 plant productivity have been observed at elevated [CO2] (Leakey et al. 2006; Kim et al. 2007). As a C4 plant, the modest increase in growth is likely due to the indirect enhancement of water-use efficiency through reduced stomatal conductance and transpiration rather than a direct increase in photosynthesis (Leakey et al. 2006; Allen et al. 2011; Prior et al. 2011). Because of the CO2-concentrating mechanism in C4 plants that mitigates the limitations of high photorespiration, maize is already functioning at near-saturated photosynthetic capacity and does not directly benefit from CO2 fertilization. Nevertheless, since maize is a summer grown crop and is anticipated to experience more frequent episodes of drought, enhanced water-use efficiency will benefit vegetative growth. However, this benefit will not likely compensate for the detrimental physiological costs of warmer temperatures and severe drought on maize productivity because the adverse impacts of these stresses are greatest on the plants reproductive processes (Prasad et al. 2006; Hatfield 2016).
In addition to the impact of abiotic stress on maize, climate change is also predicted to alter the geographical distribution, population dynamics, abundance, and aggressiveness of many important agricultural pests (Elad and Pertot 2014; Sharma 2014). For example, populations of corn earworm (Helicoverpa zea), European corn borer (Ostinia nubilalis), and corn rootworm (Diabrotica spp.) are projected to become more widespread (Porter et al. 1991; Aragón and Lobo 2012; Sharma 2014). Warmer temperatures combined with extreme precipitation or drought are also projected to increase incidence of maize fungal infection and mycotoxin contamination further reducing yield quantity and quality (Diffenbaugh et al. 2008; Wu et al. 2011; Sharma 2014). Aspergillus flavus and Fusarium verticillioides are of particular concern because they are most aggressive under warmer, dry conditions (Paterson and Lima 2010, 2011). Another concern is that a new Fusarium graminearum population, which may be more resilient to extreme weather conditions, appears to be spreading throughout some regions of the U.S. and Canada (Ward et al. 2008; Vujanovic et al. 2012; Vaughan et al. 2016a). Insect pests, pathogens and weeds typically have shorter life cycles and higher reproductive rates that enable them to adapt faster to climate changes than planted crops (Diffenbaugh et al. 2008; Manea et al. 2011; Runion et al. 2014; Sharma 2014). Consequently, future maize crops will be challenged by intensified biotic stress as well as abiotic stress (Hatfield et al. 2011).
In order to cope with the multitude of abiotic and biotic stress factors in the surrounding environment, plants have evolved sophisticated phytochemical defenses that contribute to resilience. The elucidation of plant defense efficacy requires an understanding of the precise spatial and temporal context in which the diverse protective phytochemicals are synthesized and accumulated. Upon stress perception, conserved signal transduction cascades, including specific ion channels, kinase cascades, reactive oxygen species and phytohormone signaling pathways, are activated and lead to molecular modifications that in turn activate or repress specific phytochemical biosynthetic machinery. The phytohormone abscisic acid (ABA) predominantly controls abiotic stress responses, while salicylic acid (SA) and jasmonic acid (JA)/ethylene signaling pathways mediate biotic stress resistance. The interactions between stress signaling pathways have been the focus of extensive scientific studies and reviews (Fujita et al. 2006, 2009; Robert-Seilaniantz et al. 2011; Atkinson and Urwin 2012; Rejeb et al. 2014; Foyer et al. 2016; Verma et al. 2016). An individual stressor can activate common or antagonistic pathways, resulting in cross-tolerance or cross-talk with other stress responses (Foyer et al. 2016). For example, SA usually has an antagonistic effect on JA defense responses, while ABA frequently has a positive effect on JA mediated defenses. Recent findings also suggest that ABA is central in the fine-tuning of observed responses to simultaneous biotic and abiotic stresses. Nevertheless, it is evident that the efficacy of the defense response depends on the plant species and developmental stage, as well as the nature, timing, and degree of the interacting stress stimuli (Rejeb et al. 2014).
Maize produces many different types of resilience/resistance compounds including defense-related proteins, flavonoids, and cell wall components such as lignin. However, in the present paper, we focus on three specific classes of maize defense metabolites, namely, benzoxazinoids, volatile organic compounds, and terpenoid phytoalexins. We review what is known about how abiotic stress conditions influence these maize defense metabolites and discuss knowledge gaps and potential future challenges facing maize productivity.
Benzoxazinoids
The most extensively studied class of defense-related phytochemicals in maize are the benzoxazinoids. Benzoxazinoids are nitrogen containing, indole-derived compounds based on a 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one skeleton and are associated with a wide variety of general defense activities against bacteria, fungi, insect herbivores and competing plants (Niemeyer 2009; Wouters et al. 2016). In addition to direct defense activity, benzoxazinoids have also been implicated in endogenous signaling functions regulating innate immunity and callose deposition (Ahmad et al. 2011). Benzoxazinoids accumulate as glucoside conjugates with 2-(2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one)-beta-d-glucopyranose (DIMBOA-Glc) and 2-(2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one)-beta-d-glucopyranose (HDMBOA-Glc) predominating (Niemeyer 2009; Wouters et al. 2016). These benzoxazinoid glucosides are largely considered phytoanticipins due to their accumulation in young tissues where they serve as a defensive reserve that can be quickly activated upon attack. However, consistent with inducible defenses, HDMBOA-Glc rapidly accumulates following caterpillar herbivory and pathogen infection via both de novo biosynthesis and the O-methylation of existing DIMBOA-Glc pools (Oikawa et al. 2004; Dafoe et al. 2011; Huffaker et al. 2011a). In response to cellular damage, plastid-localized glucosidases come into contact with the vacuole-localized β-glycosides to liberate the corresponding aglycones (Glenn et al. 2002; Niemeyer 2009). Both DIMBOA and HDMBOA aglycones have a short in planta half-life and quickly degrade into a complex array of further reactive products. Among the stable and quantifiable breakdown products, 6-methoxy-benzoxazolin-2-one (MBOA) is considered an effective insect-deterrent and antibiotic compound (Richardson and Bacon 1993; Bravo et al. 1997; Glenn et al. 2002; Meihls et al. 2012).
The concentration and composition of benzoxazinoids varies with maize genotype, organ and age, but is also dependent on weather conditions such as those associated with climate change (Niemeyer 2009). Warmer temperatures increase the rate of maize growth and development (Hatfield and Prueger 2015), which can influence tissue benzoxazinoid content because concentrations are highest in seedlings and continually decline with plant maturity (Cambier et al. 2000). Correspondingly, studies conducted in wheat indicate that temperature induced changes in growth rate influenced both constitutive and inducible benzoxazinoid defenses (Gianoli and Niemeyer 1996, 1997). Warmer soil temperatures and changes in moisture content also have the potential to expedite the degradation of maize root benzoxazinoid exudates leading to changes in allelopathic potential, belowground organ susceptibility and microbial communities of the rhizosphere (Woodward et al. 1978; Neal et al. 2012). Conditions of drought that restrict maize growth have been shown to increase total seedling DIMBOA concentrations (Richardson and Bacon 1993). Furthermore, Erb et al., demonstrated that drought induced ABA signaling leads to the accumulation of DIMBOA and other phenolic compounds in maize leaves (Erb et al. 2009a; b; 2011). On the other hand, conditions of drought in combination elevated [CO2] resulted in reduced constitutive levels of DIMBOA-Glc and HDMBOA-Glc in maize stem tissues (Vaughan et al. 2016b), but the aglycones were not measured in this study so direct comparisons are not possible. Further research is needed to determine how differences in the composition and quantities of individual benzoxazinoids will ultimately influence maize susceptibility to pests.
The production potential of nitrogen containing metabolites such as benzoxazinoids are dependent on soil nitrogen availability, and future climate conditions are projected to increase soil erosion and deplete fertility (Brevik 2013). However, while soil nitrogen deficiency reduces maize leaf DIMBOA content, low phosphorous increases the amount of DIMBOA (Schlüter et al. 2013). In the absence of significant fertilizer applications, both nitrogen and phosphorous are projected to become increasingly limited under conditions of elevated [CO2] (Niklaus and Körner 2004). Additional research investigating the interactive effects of both low nitrogen and phosphorous on benzoxazinoids will be necessary to fully understand the potential impact of depleted soil fertility in the context of climate change.
Given the limited amount of data available, it is difficult to predict the impact of future climate conditions on maize benzoxazinoid defenses. Obviously the timing and location of pathogen or insect herbivore attack influences the outcome, but under conditions of drought benzoxazinoid defenses will likely be stimulated in maize leaf tissues. Even under warmer temperatures the growth rate of maize will be limited by water availability. A stimulation in benzoxazinoids, may provide enhanced protection against insect and pathogen invaders which are sensitive to these compounds. However, the drought stimulated enhancement of benzoxazinoids and other phenolic compounds alone were not sufficient to induce resistance against the generalist herbivore Spodoptera littoralis, nor the necrotrophic pathogen Setosphaeria turcica (Erb et al. 2011). Furthermore, it is unclear how the interaction between elevated [CO2] and drought will influence the entire array of benzoxazinoid metabolites and complex pathway regulation.
Volatile organic compounds
Maize has been widely studied as a model for the production of volatile organic compounds (VOCs) that function as an airborne plant intra-and interspecies communication system (Turlings et al. 1990; Engelberth et al. 2004; Ton et al. 2007). Maize VOCs are structurally diverse and include indole, C6 green leaf volatiles (GLVs), and multiple terpenoids: C10 monoterpenes, C15 sesquiterpenes, homoterpenes (comprising C11 (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and C16 (3E,7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT)), and C20 diterpenes. Plants constitutively emit low levels of specific volatiles, but unique blends are induced by different types of stress and play an important role in both defense signaling and resistance. For example, the emission of GLVs and indole from herbivore damaged maize leaves has been shown to prime the defenses of distal tissues or neighboring plants resulting in a stronger and/or faster defense response upon subsequent attack (Engelberth et al. 2004; Erb et al. 2015). Additionally, herbivore-induced volatiles function in indirect defense via the attraction of natural enemies of the herbivore. For instance, maize leaves damaged by Spodoptera exigua (beet armyworm) larvae emit VOCs that attract female Cotesia marginiventris parasitic wasps, which lay eggs in the larvae causing their untimely death and consequently reduced plant damage/consumption (Turlings et al. 1990, 1991). Nevertheless, since VOCs are indiscriminately released into the plants surroundings they can also be recognized and used by insects to locate preferred host plants. For example, gravid female moths of Ostrinia nubilalis (European corn borer) are attracted to the maize GLVs nonanal and decanal (Molnar et al. 2015).
There is a large amount of variability in the composition and quantity of constitutive and inducible VOCs produced by different maize cultivars and lines (Turlings et al. 1998; Kollner et al. 2009; Degen et al. 2012); however, environmental conditions can also contribute to variation in VOC emissions. Soil moisture, air humidity, temperature, and light intensity all have been shown to influence maize VOCs. In response to induction by Spodoptera littoralis oral secretions, volatile emissions from young maize leaves were greatest at 60% relative air humidity and between 22 and 27 °C (Gouinguene and Turlings 2002). However, the relative abundance of individual volatile compounds varied at different temperatures (Gouinguene and Turlings 2002). Although temperature can affect the vapor pressure of volatiles, it was speculated that changes in emission of the induced volatiles with temperature and humidity was due to changes in stomatal aperture. Indeed, stomata are thought to be the main route of exit for many leaf volatiles (Widhalm et al. 2015). In support of this hypothesis, maize leaf treatment with ABA or dark exposure at midday (two treatments that reduce stomatal aperture) reduced the emission of E-β-farnesene (Seidl-Adams et al. 2015). However, the effect of stomatal conductance on volatile release depends on the chemical properties of the individual volatile, and emission of some volatiles have been found to be more dependent on stomatal aperture than others (Fall and Monson 1992; Nemecek-Marshall et al. 1995). Interestingly, Gouinguene and Turlings (2002) reported that elicited maize volatiles were greater from plants in dry soils than in wet soils; however, while the amount of DMNT and indole emitted was greatest at 20–40% soil moisture, the concentration of (E)-β-caryophyllene and bergamotene was greatest at 80–100% soil moisture. Nevertheless, given that a natural physiological stress response of plants to elevated [CO2] and drought is the reduction of stomata aperture, these abiotic stress conditions likely hinder the release of numerous maize volatiles that regulate multi-trophic interactions.
Volatile organic compounds are also emitted from maize roots. In response to root attack by the specialist maize herbivore Diabrotica virgifera virgifera (Western corn rootworm), roots produce the sesquiterpene (E)-β-caryophyllene that attracts the entomopathogenic nematode Heterorhabditis megidis (Rasmann et al. 2005). However, D. virgifera can also use maize root volatiles, specifically the gaseous hormone ethylene and (E)-β-caryophyllene, to select the most suitable host plants (Robert et al. 2012). Therefore, herbivore-induced volatiles in maize roots play similar roles as their aboveground counterparts. (E)-β-caryophyllene diffuses though the soil in the gas phase and predictably low soil moisture facilitates diffusion. However, at extremely low levels of soil moisture, (E)-β-caryophyllene is rapidly lost into the atmosphere through vertical diffusion (Hiltpold and Turlings 2008). Drought and warmer soil temperatures will likely increase the rapid loss of root-derived volatile signals leading to changes in belowground multi-trophic interactions and defenses.
As discussed in the context of benzoxazinoids, the indirect influence of future climate conditions on soil fertility may also reduce maize VOC production. Maize supplemented with a complete nutrient solution released significantly more constitutive and induced volatile organic compounds (Gouinguene and Turlings 2002). Therefore, without increased fertilization, soil erosion may further compromise maize VOC based defenses.
Given that different weather conditions can have profound effects on the emission and dispersal of VOCs, understanding the impact of climate variability on the biosynthesis of these VOCs is more complex. For example, the measurement of emitted volatiles from the head space alone may not represent the total quantity synthesized under the different environmental conditions. A more comprehensive approach is necessary. Such as the methods used by Seidl-Adams et al. (2015) that included analyses of biosynthetic gene expression and quantification of both volatiles emitted and intracellular volatile accumulation. Further studies using this approach are required to fully differentiate between environmental effects on volatile biosynthesis with respect to measured volatile emission.
In addition to effecting volatile production and release, weather conditions can also affect the unique chemistry of VOCs. Many abiotic stresses lead to enhanced oxidative stress that may result in oxidation of certain volatiles changing their ability to act as airborne signals (Niinemets et al. 2014). Furthermore, changes in atmospheric gases, particularly the presence of pollutants impacts the chemistry of many volatiles after they have been released from the plant. For instance, moderately enhanced atmospheric ozone levels result in the degradation of many herbivore induced terpenes and GLVs (Pinto et al. 2007). The potential effects of climate change on volatiles from a variety of plant species were recently reviewed (Blande et al. 2014).
Despite the insights provided by published research, additional information is still required to determine how the interactions of multiple simultaneous abiotic and biotic stress factors will influence maize VOC production and emission. Furthermore, it remains unclear how climate induced changes in VOC production will influence the web of ecological interactions with maize. While climate can directly shape the community structure surrounding maize crops, it can also indirectly alter plant produced signals that are involved in the attraction and/or repellence of interacting insects and microbes, both which in turn can further dictate community structure and influence maize productivity. Therefore, ecological studies paired with genetic mechanisms and resources are an essential combination to fully understand and demonstrate how climate induced changes in maize VOCs relate to field relevant resilience. An added layer of complexity is the ability of insects to learn chemical cues through positive and negative associations (Paré and Tumlinson 1999; Jones and Agrawal 2017). Altered differences in the rates of development, thermal tolerance and associative learning between parasitoids, predators and herbivores could have further consequences during climate change (Eigenbrode et al. 2015).
Terpenoid phytoalexins
Compared to the maize benzoxazinoid pathway, similarly complex arrays of non-volatile terpenoid phytoalexins exist yet were first discovered only recently in 2011 (Huffaker et al. 2011b; Schmelz et al. 2011). Recent analyses of pathway mutants suggest important and unexpected roles in maize resilience (Vaughan et al. 2015). To date, maize terpenoid phytoalexins consist of zealexins, which are sesquiterpene acids on a β-macrocarpene carbon skeleton and kauralexins which are acidic diterpenoid ent-kaura(e)ne derivatives. Both zealexin and kauralexins contain numerous related family members that display significant antifungal activity against various maize pathogenic fungi (Huffaker et al. 2011b; Schmelz et al. 2011, 2014). Additionally, kauralexins can be induced by O. nubilalis stem tunneling and function as local insect antifeedants (Schmelz et al. 2011). While strongly pathogen-inducible, kauralexins are also unexpectedly produced in roots during drought stress and play a measurable role in drought tolerance (Vaughan et al. 2015). Furthermore, the abundance of zealexin and kauralexin phytoalexins can vary dependent upon the type of stress. For instance, the concentration of total zealexins is greatest in response to pathogen infection, but in response to herbivore damage or drought the kauralexins comparatively predominate (Huffaker et al. 2011b; Schmelz et al. 2011; Vaughan et al. 2015).
As inducible defense metabolites synthesized de novo in response to stimuli, the accumulation of terpenoid phytoalexins is subject to cross-talk between signaling pathways during conditions of multiple stress factors. In maize stalks, the biosynthesis of the terpenoid phytoalexins is promoted by the synergistic interactions between of jasmonic acid (JA) and ethylene (Schmelz et al. 2011, 2014). However, belowground treatment with ABA alone is sufficient to induce the accumulation of terpenoid phytoalexins of maize roots (Vaughan et al. 2015), suggesting that regulation may vary among organs. Further research is needed to verify the organ specific involvement of the different phytohormone pathways regulating terpenoid phytoalexin production.
Several publications investigating the influence of elevated [CO2] and drought on the accumulation maize terpenoid phytoalexins have recently been published (Vaughan et al. 2014, 2015, 2016b). Since multiple variables were evaluated in these studies, the data obtained from maize stem inoculations with Fusarium verticillioides under the different abiotic stress conditions have been compiled into a heat map (Fig. 1). Interestingly, growth at elevated [CO2] compromised the induction of both jasmonic acid (JA) and salicylic acid (SA) in maize (Vaughan et al. 2014). This is unlike soybean plants that exhibit an increase in SA while JA signaling is dampened (Casteel et al. 2012). Consistent with compromised JA signaling, the accumulation of downstream terpenoid phytoalexins was also reduced at elevated [CO2], and the maize plants were more susceptible to pathogen proliferation.
Although drought has the potential to enhance JA signaling (Riemann et al. 2015; Vaughan et al. 2016b), the addition of drought stress did not negate the compromising effects of elevated [CO2] on the maize defense response. Despite the more rapid and higher accumulation of JA under conditions of drought, maize under conditions of elevated [CO2] with and without drought stress accumulated less terpenoid phytoalexins than irrigated plants at ambient [CO2]. Interestingly, there were also significantly less terpenoid phytoalexins in the inoculated tissue of drought stressed plants at ambient [CO2] in comparison to irrigated plants at ambient [CO2] (Vaughan et al. 2016b). However, since the accumulation of terpenoid phytoalexins is also dependent on the concentration and early progression of the inoculum (Huffaker et al. 2011b), this result may be attributed to less pathogen proliferation due to drought-related hydraulic changes in the stem tissue that were non-conducive to the pathogen growth (Vaughan et al. 2016b). Comparative works with heat killed fungal spores could resolve innate differences in basal defense elicitation caused by abiotic variables.
Belowground kauralexin accumulation in response to Diabrotica balteata larval feeding damage was also reduced at elevated [CO2] under well-watered conditions (Vaughan et al. 2014). However, given that terpenoid phytoalexins are induced in root tissues by drought stress and this induction is mediated by ABA, the compromising effects of elevated [CO2] on JA and kauralexin accumulation in response to D. balteata larval feeding damage was negated under the combined conditions of elevated [CO2] and drought (Vaughan et al. 2016b).
Vast knowledge gaps remain in our understanding of how future climate variability will impact inducible maize defenses, such as terpenoid phytoalexins. Additional research is needed to evaluate the role of terpenoid phytoalexins in abiotic stress and determine the mechanism(s) involved in kauralexin mediated drought stress tolerance. The impact of warmer temperatures on maize terpenoid phytoalexins also remains to be determined. JA signaling can be upregulated in response to heat stress and is thought to play a role in thermotolerance of some plants (Sharma and Laxmi 2016). The impact of individual and combined abiotic and biotic stress factors on ethylene signaling is another area of research worthy of consideration. It is possible that the induction of JA and ethylene with heat stress will negate the effects of elevated [CO2] alone and in interaction with drought.
Knowledge gaps and conclusions
A simplified summary of the effects of individual and combined abiotic stress factors on maize phytochemical defenses was constructed (Fig. 2). This overview integrates both constitutive and induced defense responses to broadly capture empirical data relating to the potential impact of various abiotic stressors associated with climate change on the listed classes of phytochemicals. At first glance, it is evident that numerous knowledge gaps exist, particularly in understanding the interactive effects of multiple simultaneous abiotic stress conditions on maize phytochemicals. This is of particular concern given that future climate predictions suggest that our agricultural systems will more frequently be challenged by a combination of multiple abiotic stressors (elevated [CO2], drought, and heat). Additionally, the influence of the abiotic stress on maize phytochemicals will further depend on the organ type and developmental stage. Research has primarily focused on the aboveground portions of younger maize plant tissues, and extrapolations to other tissues or mature maize plants may not be possible at this time.
While an individual maize plant is likely to experience multiple abiotic stress conditions in a given year, changes in atmospheric composition will be the most consistent stress. Overall, elevated [CO2] appears to compromise maize phytochemical defenses suggesting that future maize plants will be more susceptible to biotic stress. Drought and heat stress have a positive to mixed effect on the phytochemical defenses depending in part upon on the degree of the stress. Under conditions of drought, maize appears to modulate its phytochemical defenses by organ type. Aboveground the benzoxazinoid defenses are stimulated, but belowground in the roots terpenoid phytoalexins are predominantly deployed. The susceptibility or resistance of drought stressed maize will ultimately depend on the sensitivity of the pest or pathogen to the major class of defenses deployed within that particular tissue. For example, the strategy of enhanced aboveground benzoxazinoid defenses will not protect maize against the mycotoxigenic fungus F. verticillioides, which is capable of detoxifying benzoxazinoids (Glenn et al. 2001, 2002), but displays reduced growth in the presence of terpenoid phytoalexins (Vaughan et al. 2014). Indeed, F. verticillioides has been shown to thrive on drought stressed maize (Miller 2001). Likewise, maize plants containing high levels of HDMBOA-Glc are susceptible to maize leaf aphid (Rhopalosiphum maidis) due to the reduced presence of free DIMBOA and less effective promotion of callose deposition around the feeding site (Meihls et al. 2013). Thus the fine tuning of defense modulation will shape the biodiversity and abundance of pests within the maize agroecosystem.
Interestingly, the abiotic stress in direct contact with a tissue type appears to have the most influence on that tissue type, and the impact of that particular stress factor takes precedence over the other stress factors. Elevated [CO2] is in direct contact with the aboveground tissues, and reduced soil moisture is directly associated with the roots. Aboveground the compromising effects of elevated [CO2] on maize phytochemicals takes precedence over any potential stimulation caused by drought. Belowground, the influence of drought on roots appears to supersede the impact of elevated [CO2]. While additional research including other interacting abiotic stress factors will be necessary to test this hypothesis, it is consistent with the ‘optimal defense theory’ which predicts that defense-related phytochemicals will be allocated to tissues of greatest value or risk of attack (Zangerl and Rutledge 1996). The assignment of which tissue is of greatest value or risk is not necessarily fixed and can be adjusted based on the plant’s status. Therefore, the outcome of defense signaling cross-talk in different tissues may also depend on the status of that particular tissue and its association to the interacting stress factors.
Understanding the effects of climate changes on maize phytochemicals and the ultimate impact of these effects on maize productivity remains challenging as integrated interactions are quite complex. The interactive effects of elevated [CO2], drought, and heat on maize constitutive and induced phytochemical defenses have not been investigated. Furthermore, induced phytochemical responses may vary with the type of biotic stress interacting with the abiotic stress factors.
Nevertheless, it is evident that weather induced changes in maize phytochemical defense responses will ultimately influence maize productivity. Given that climate simulation models predict a 15–50% decrease in maize yields from the twentieth to the twenty-first century (Xu et al. 2016), and most of these models do not account for the potential impact of extra biotic stress (Chakraborty and Newton 2011), filling the many knowledge gaps is a well merited urgent task. Despite advancements in crop protection methods, pests (including weeds, herbivores, and pathogens) are estimated to account for approximately 30% of global maize crops losses (Oerke 2006). Maize losses related to herbivores and pathogens display large regional variability. This is thought to be due to the climate restricted geographical distribution of aggressive pests (downy mildews, corn borers) that localize to particular hot spots (Oerke 2006). Climate change will not only widen the area of such potential hot spots, but also compromise natural maize phytochemical defenses that have the potential to limit crop losses to pests.
Effective strategies to improve the resilience of maize productivity in the face of climate changes will have to incorporate knowledge of both abiotic and biotic stress tolerance mechanisms. Although the phytochemicals discussed here, were classically referred to as ‘secondary metabolites’ as they did not appear to have any fundamental roles in plant growth, they are now viewed as specialized metabolites that are essential in plant-environment interactions that mediate adaptation, defense, long-term survival (Ramakrishna and Ravishankar 2011). Therefore, defense metabolites will be a critical component of climate resilience of maize. The recently discovered terpenoid phytoalexins are promising metabolites that function in both abiotic and biotic stress tolerance (Vaughan et al. 2015) and may aid in improving maize resilience to both drought and mycotoxigenic pathogens. Nevertheless, a deeper understanding of diverse maize phytochemical responses to combined abiotic and biotic stress will be required for ensuring and improving future maize productivity.
References
Ahmad S, Veyrat N, Gordon-Weeks R, Zhang Y, Martin J, Smart L, Glauser G, Erb M, Flors V, Frey M, Ton J (2011) Benzoxazinoid metabolites regulate innate immunity against aphids and fungi in maize. Plant Physiol 157:317–327
Allen LH Jr, Kakani VG, Vu JCV, Boote KJ (2011) Elevated CO2 increases water use efficiency by sustaining photosynthesis of water-limited maize and sorghum. J Plant Physiol 168:1909–1918
Aragón P, Lobo JM (2012) Predicted effect of climate change on the invasibility and distribution of the Western corn root-worm. Agric For Entomol 14:13–18
Atkinson NJ, Urwin PE (2012) The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63:3523–3543
Barton B, Clark SE (2014) Water and climate risks facing U.S. corn production: how companies and investors can cultivate sustainability. A CERES Report, Boston
Bienkowski B (2012) U.S. Drought 2012: pick your poison. Scientific American. The daily climate. https://www.scientificamerican.com/article/us-drought-2012-pick-your-poison/
Blande JD, Holopainen JK, Niinemets U (2014) Plant volatiles in polluted atmospheres: stress responses and signal degradation. Plant Cell Environ 37:1892–1904
Block A, Vaughan MM, Christensen SA, Alborn HT, Tumlinson JH (2017) Elevated carbon dioxide reduces emission of herbivore induced volatiles in Zea mays. Plant Cell Environ. doi:10.1111/pce.12976
Bravo HR, Copaja SV, Lazo W (1997) Antimicrobial activity of natural 2-benzoxazolinones and related derivatives. J Agric Food Chem 45:3255–3257
Brevik E (2013) The potential impact of climate change on soil properties and processes and corresponding influence on food security. Agriculture 3:398
Cambier V, Hance T, de Hoffmann E (2000) Variation of DIMBOA and related compounds content in relation to the age and plant organ in maize. Phytochemistry 53:223–229
Casteel C, Niziolek O, Leakey A, Berenbaum M, DeLucia E (2012) Effects of elevated CO2 and soil water content on phytohormone transcript induction in Glycine max after Popillia japonica feeding. Arthropod Plant Interact 6:1–9
Chakraborty S, Newton AC (2011) Climate change, plant diseases and food security: an overview. Plant Pathol 60:2–14
Dafoe NJ, Huffaker A, Vaughan MM, Duehl AJ, Teal PE, Schmelz EA (2011) Rapidly induced chemical defenses in maize stems and their effects on short-term growth of Ostrinia nubilalis. J Chem Ecol 37:984–991
Degen T, Bakalovic N, Bergvinson D, Turlings TC (2012) Differential performance and parasitism of caterpillars on maize inbred lines with distinctly different herbivore-induced volatile emissions. PLoS ONE 7:e47589
Diffenbaugh NS, Krupke CH, White MA, Alexander CE (2008) Global warming presents new challenges for maize pest management. Environ Res Lett 3:044007
Eigenbrode SD, Davis TS, Crowder DW (2015) Climate change and biological control in agricultural systems: principles and examples from North America. In: Björkman C, Niemelä P (eds) Climate change and insect pests. CABI, Wallingford
Elad Y, Pertot I (2014) Climate change impacts on plant pathogens and plant diseases. J Crop Improv 28:99–139
Engelberth J, Alborn HT, Schmelz EA, Tumlinson JH (2004) Airborne signals prime plants against insect herbivore attack. Proc Natl Acad Sci 101:1781–1785
Erb M, Flors V, Karlen D, de Lange E, Planchamp C, D’Alessandro M, Turlings TCJ, Ton J (2009a) Signal signature of aboveground-induced resistance upon belowground herbivory in maize. Plant J 59:292–302
Erb M, Gordon-Weeks R, Flors V, Camanes G, Turlings TCJ, Ton J (2009b) Belowground ABA boosts aboveground production of DIMBOA and primes induction of chlorogenic acid in maize. Plant Signal Behav 4:636–638
Erb M, Kollner TG, Degenhardt J, Zwahlen C, Hibbard BE, Turlings TC (2011) The role of abscisic acid and water stress in root herbivore-induced leaf resistance. New Phytol 189:308–320
Erb M, Veyrat N, Robert CA, Xu H, Frey M, Ton J, Turlings TC (2015) Indole is an essential herbivore-induced volatile priming signal in maize. Nat Commun 6:6273
Fall R, Monson RK (1992) Isoprene emission rate and intercellular isoprene concentration as influenced by stomatal distribution and conductance. Plant Physiol 100:987–992
Foyer CH, Rasool B, Davey JW, Hancock RD (2016) Cross-tolerance to biotic and abiotic stresses in plants: a focus on resistance to aphid infestation. J Exp Bot 67:2025–2037
Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, Shinozaki K (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol 9:436–442
Fujita Y, Fujita M, Yamaguchi-Shinozaki K, Shinozaki K (2009) Transcription factors involved in the crosstalk between abiotic and biotic stress-signaling networks. Signal crosstalk in plant stress responses. Wiley-Blackwell, Hoboken, pp 43–58
Gianoli E, Niemeyer HM (1996) Environmental effects on the induction of wheat chemical defences by aphid infestation. Oecologia 107:549–552
Gianoli E, Niemeyer HM (1997) Environmental effects on the accumulation of hydroxamic acids in wheat seedlings: the importance of plant growth rate. J Chem Ecol 23:543–551
Glenn AE, Hinton DM, Yates IE, Bacon CW (2001) Detoxification of corn antimicrobial compounds as the basis for isolating Fusarium verticillioides and some other Fusarium species from corn. Appl Environ Microbiol 67:2973–2981
Glenn AE, Gold SE, Bacon CW (2002) Fdb1 and Fdb2, Fusarium verticillioides loci necessary for detoxification of preformed antimicrobials from corn. Mol Plant Microbe Interact 15:91–101
Gouinguene SP, Turlings TC (2002) The effects of abiotic factors on induced volatile emissions in corn plants. Plant Physiol 129:1296–1307
Hatfield JL (2016) Increased temperatures have dramatic effects on growth and grain yield of three maize hybrids. The American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America Inc, Madison
Hatfield JL, Prueger JH (2015) Temperature extremes: Effect on plant growth and development. Weather Clim Extremes 10:4–10
Hatfield JL, Boote KJ, Kimball BA, Ziska LH, Izaurralde RC, Ort D, Thomson AM, Wolfe D (2011) Climate impacts on agriculture: implications for crop production. Agronomy J 103:351–370
Hiltpold I, Turlings TC (2008) Belowground chemical signaling in maize: when simplicity rhymes with efficiency. J Chem Ecol 34:628–635
Huffaker A, Dafoe NJ, Schmelz EA (2011a) ZmPep1, an ortholog of Arabidopsis elicitor Peptide 1, regulates maize innate immunity and enhances disease resistance. Plant Physiol 155:1325–1338
Huffaker A, Kaplan F, Vaughan MM, Dafoe NJ, Ni X, Rocca JR, Alborn HT, Teal PEA, Schmelz EA (2011b) Novel acidic sesquiterpenoids constitute a dominant class of pathogen-induced phytoalexins in maize. Plant Physiol 156:2082–2097
Jones PL, Agrawal AA (2017) Learning in insect pollinators and herbivores. Ann Rev Entomol 62:53–71
Karl TR, Melillo JM, Peterson TC (eds) (2009) Global climate change impacts in the United States. Cambridge University Press, New York
Kim S-H, Gitz DC, Sicher RC, Baker JT, Timlin DJ, Reddy VR (2007) Temperature dependence of growth, development, and photosynthesis in maize under elevated CO2. Environ Exp Bot 61:224–236
Kollner TG, Gershenzon J, Degenhardt J (2009) Molecular and biochemical evolution of maize terpene synthase 10, an enzyme of indirect defense. Phytochemistry 70:1139–1145
Leakey ADB, Uribelarrea M, Ainsworth EA, Naidu SL, Rogers A, Ort DR, Long SP (2006) Photosynthesis, productivity and yield of maize are not affected by open-air elevation of CO2 concentration in the absence of drought. Plant Physiol 140:106
Manea A, Leishman MR, Downey PO (2011) Exotic C4 grasses have increased tolerance to glyphosate under elevated carbon dioxide. Weed Sci 59:28–36
Meihls LN, Kaur H, Jander G (2012) Natural variation in maize defense against insect herbivores. Cold Spring Harb Symp Quant Biol 77:269–283
Meihls LN, Handrick V, Glauser G, Barbier H, Kaur H, Haribal MM, Lipka AE, Gershenzon J, Buckler ES, Erb M, Köllner TG, Jander G (2013) Natural variation in maize aphid resistance is associated with 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside methyltransferase activity. Plant Cell 25:2341–2355
Miller JD (2001) Factors that affect the occurrence of fumonisin. Environ Health Perspect 109:321–324
Molnar BP, Toth Z, Fejes-Toth A, Dekker T, Karpati Z (2015) Electrophysiologically-active maize volatiles attract gravid female European Corn Borer, Ostrinia nubilalis. J Chem Ecol 41:997–1005
Neal AL, Ahmad S, Gordon-Weeks R, Ton J (2012) Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere. PLoS ONE 7:e35498
Nemecek-Marshall M, MacDonald RC, Franzen JJ, Wojciechowski CL, Fall R (1995) Methanol emission from leaves (enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomatal conductance and leaf development). Plant Physiol 108:1359–1368
Niemeyer HM (2009) Hydroxamic acids derived from 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one: key defense chemicals of cereals. J Agric Food Chem 57:1677–1696
Niinemets U, Fares S, Harley P, Jardine KJ (2014) Bidirectional exchange of biogenic volatiles with vegetation: emission sources, reactions, breakdown and deposition. Plant Cell Environ 37:1790–1809
Niklaus PA, Körner C (2004) Synthesis of a six-year study of calcareous grassland responses to in situ CO2 enrichment. Ecol Monogr 74:491–511
Oerke EC (2006) Crop losses to pests. J Agric Sci 144:31–43
Oikawa A, Ishihara A, Tanaka C, Mori N, Tsuda M, Iwamura H (2004) Accumulation of HDMBOA-Glc is induced by biotic stresses prior to the release of MBOA in maize leaves. Phytochemistry 65:2995–3001
Paré PW, Tumlinson JH (1999) Plant volatiles as a defense against insect herbivores. Plant Physiol 121:325–332
Paterson RRM, Lima N (2010) How will climate change affect mycotoxins in food? Food Res Int 43:1902–1914
Paterson RRM, Lima N (2011) Further mycotoxin effects from climate change. Food Res Int 44:2555–2566
Petzold-Maxwell JL, Meinke LJ, Gray ME, Estes RE, Gassmann AJ (2013) Effect of Bt maize and soil insecticides on yield, injury, and rootworm survival: implications for resistance management. J Econ Entomol 106:1941–1951
Pinto DM, Blande JD, Nykanen R, Dong WX, Nerg AM, Holopainen JK (2007) Ozone degrades common herbivore-induced plant volatiles: Does this affect herbivore prey location by predators and parasitoids? J Chem Ecol 33:683–694
Porter JH, Parry ML, Carter TR (1991) The potential effects of climatic change on agricultural insect pests. Agric For Meteorol 57:221–240
Prasad PVV, Boote KJ, Allen LH Jr (2006) Adverse high temperature effects on pollen viability, seed-set, seed yield and harvest index of grain-sorghum [Sorghum bicolor (L.) Moench] are more severe at elevated carbon dioxide due to higher tissue temperatures. Agric For Meteorol 139:237–251
Prior SA, Runion GB, Marble SC, Rogers HH, Gilliam CH, Torbert HA (2011) A review of elevated atmospheric CO2 effects on plant growth and water relations: implications for horticulture. HortScience 46:158–162
Ramakrishna A, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 6:1720–1731
Rasmann S, Kollner TG, Degenhardt J, Hiltpold I, Toepfer S, Kuhlmann U, Gershenzon J, Turlings TC (2005) Recruitment of entomopathogenic nematodes by insect-damaged maize roots. Nature 434:732–737
Rejeb I, Pastor V, Mauch-Mani B (2014) Plant responses to simultaneous biotic and abiotic stress: molecular mechanisms. Plants 3:458
Richardson MD, Bacon CW (1993) Cyclic hydroxamic acid accumulation in corn seedlings exposed to reduced water potentials before, during, and after germination. J Chem Ecol 19:1613–1624
Riemann M, Dhakarey R, Hazman M, Miro B, Kohli A, Nick P (2015) Exploring jasmonates in the hormonal network of drought and salinity responses. Front Plant Sci 6:1077
Robert CA, Erb M, Duployer M, Zwahlen C, Doyen GR, Turlings TC (2012) Herbivore-induced plant volatiles mediate host selection by a root herbivore. New Phytol 194:1061–1069
Robert-Seilaniantz A, Grant M, Jones JDG (2011) Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism. Ann Rev Phytopath 49:317–343
Runion GB, Prior SA, Price AJ, McElroy JS, Torbert HA (2014) Effects of elevated CO2 on biomass and fungi associated with two ecotypes of ragweed (Ambrosia artemisiifolia L.). Front. Plant Sci 5:500
Schlüter U, Colmsee C, Scholz U, Bräutigam A, Weber AP, Zellerhoff N, Bucher M, Fahnenstich H, Sonnewald U (2013) Adaptation of maize source leaf metabolism to stress related disturbances in carbon, nitrogen and phosphorus balance. BMC Genom 14:442
Schmelz EA, Kaplan F, Huffaker A, Dafoe NJ, Vaughan MM, Ni XZ, Rocca JR, Alborn HT, Teal PE (2011) Identity, regulation, and activity of inducible diterpenoid phytoalexins in maize. Proc Natl Acad Sci USA 108:5455–5460
Schmelz EA, Huffaker A, Sims JW, Christensen SA, Lu X, Okada K, Peters RJ (2014) Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins. Plant J 79:659–678
Schultz R (2012) Drought creates danger of toxic fungi in surviving crops. Wis State J. Accessed Aug 13.http://host.madison.com/business/drought-creates-danger-of-toxic-fungi-in-surviving-crops/article_f930679a-e5a6-11e1-bc3d-001a4bcf887a.html.
Seidl-Adams I, Richter A, Boomer KB, Yoshinaga N, Degenhardt J, Tumlinson JH (2015) Emission of herbivore elicitor-induced sesquiterpenes is regulated by stomatal aperture in maize (Zea mays) seedlings. Plant Cell Environ 38:23–34
Sharma HC (2014) Climate change effects on insects: implications for crop protection and food security. J Crop Improv 28:229–259
Sharma M, Laxmi A (2016) Jasmonates: emerging players in controlling temperature stress tolerance. Front Plant Sci 6:1129
Smith D, Mitchell P (2012) Drought 2012: moldy corn and crop insurance. Integr Pest Crop Manag IPCM-edit Sept 24. http://ipcm.wisc.edu/blog/2012/09/drought-2012-moldy-corn-and-crop-insurance/
Solomon S, Qin D, Manning M, Chen Z, Marquis M, Averyt KB, Tignor M, Miller HL (2007) Climate change 2007: the physical science basis. Contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge, p 996
Ton J, D’Alessandro M, Jourdie V, Jakab G, Karlen D, Held M, Mauch-Mani B, Turlings TCJ (2007) Priming by airborne signals boosts direct and indirect resistance in maize. Plant J 49:16–26
Turlings TC, Tumlinson JH, Lewis WJ (1990) Exploitation of herbivore-induced plant odors by host-seeking parasitic wasps. Science 250:1251–1253
Turlings TC, Tumlinson JH, Heath RR, Proveaux AT, Doolittle RE (1991) Isolation and identification of allelochemicals that attract the larval parasitoid, Cotesia marginiventris (Cresson), to the microhabitat of one of its hosts. J Chem Ecol 17:2235–2251
Turlings TCJ, Bernasconi M, Bertossa R, Bigler F, Caloz G, Dorn S (1998) The induction of volatile emissions in maize by three herbivore species with different feeding habits: possible consequences for their natural enemies. Biol Control 11:122–129
Vaughan MM, Huffaker A, Schmelz EA, Dafoe NJ, Christensen S, Sims J, Martins VF, Swerbilow JAY, Romero M, Alborn HT, Allen LH, Teal PEA (2014) Effects of elevated [CO2] on maize defense against mycotoxigenic Fusarium verticillioides. Plant Cell Environ 37:2691–2706
Vaughan MM, Christensen S, Schmelz EA, Huffaker A, McAuslane HJ, Alborn HT, Romero M, Allen LH, Teal PEA (2015) Accumulation of terpenoid phytoalexins in maize roots is associated with drought tolerance. Plant Cell Environ 38:2195–2207
Vaughan MM, Backhouse D, Del Ponte EM (2016a) Climate change impacts on the ecology of Fusarium graminearum species complex and susceptibility of wheat to Fusarium head blight: a review. World Mycotoxin J 9:685–700
Vaughan MM, Huffaker A, Schmelz EA, Dafoe NJ, Christensen SA, McAuslane HJ, Alborn HT, Allen LH, Teal PE (2016b) Interactive effects of elevated [CO2] and drought on the maize phytochemical defense response against mycotoxigenic Fusarium verticillioides. PLoS ONE 11:e0159270
Verma V, Ravindran P, Kumar PP (2016) Plant hormone-mediated regulation of stress responses. BMC Plant Biol 16:86
Vujanovic V, Goh YK, Daida P (2012) Heat- and cold-shock responses in Fusarium graminearum 3 acetyl- and 15 acetyl-deoxynivalenol chemotypes. J Microbiol 50:97–102
Ward TJ, Clear RM, Rooney AP, O’Donnell K, Gaba D, Patrick S, Starkey DE, Gilbert J, Geiser DM, Nowicki TW (2008) An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America. Fungal Genet Biol 45:473–484
Widhalm JR, Jaini R, Morgan JA, Dudareva N (2015) Rethinking how volatiles are released from plant cells. Trends Plant Sci 20:545–550
Woodward MD, Corcuera LJ, Helgeson JP, Upper CD (1978) Decomposition of 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in aqueous solutions. Plant Physiol 61:796–802
Wouters FC, Blanchette B, Gershenzon J, Vassão DG (2016) Plant defense and herbivore counter-defense: benzoxazinoids and insect herbivores. Phytochem Rev 15:1127–1151
Wu F, Bhatnagar D, Bui-Klimke T, Carbone I, Hellmich R, Munkvold G, Paul P, Payne G, Takle E (2011) Climate change impacts on mycotoxin risks in US maize. World Mycotoxin J 4:79–93
Xu H, Twine TE, Girvetz E (2016) Climate change and maize yield in Iowa. PLoS ONE 11:e0156083
Zangerl AR, Rutledge CE (1996) The probability of attack and patterns of constitutive and induced defense: a test of optimal defense theory. Am Nat 147:599–608
Zukoff SN, Ostlie KR, Potter B, Meihls LN, Zukoff AL, French L, Ellersieck MR, Wade French B, Hibbard BE (2016) Multiple assays indicate varying levels of cross resistance in Cry3Bb1-selected field populations of the western corn rootworm to mCry3A, eCry3.1Ab, and Cry34/35Ab1. J Econ Entomol 109:1387–1398
Acknowledgements
We are exceptionally grateful to Dr. Dorothea Tholl, Dr. Argelia Lorence, and Dr. Susan McCormick for their selfless and encouraging mentorship. We also thank Dr. Paul Shirk, Dr. Matt Bakker, and Dr. Todd Ward for aiding with manuscript suggestions, proofreading, and revisions. We also extend our gratitude to the Phytochemical Society of North America for inviting us to contribute this review article. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer.
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Vaughan, M.M., Block, A., Christensen, S.A. et al. The effects of climate change associated abiotic stresses on maize phytochemical defenses. Phytochem Rev 17, 37–49 (2018). https://doi.org/10.1007/s11101-017-9508-2
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DOI: https://doi.org/10.1007/s11101-017-9508-2