Abstract
The effect of 8-isoprostanes on potassium (K+)-depolarization-evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K+-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF2α(1–100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM–30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF2α was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF2α(1–30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF2α exhibits a dual regulatory effect on K+-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF 2α is due to the activation of EP1/EP2 receptors while the excitatory effects are due to the activation of thromboxane receptors.
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Opere, C.A., Zheng, W.D., Huang, J. et al. Dual Effect of Isoprostanes on the Release of [3H]D-Aspartate from Isolated Bovine Retinae: Role of Arachidonic Acid Metabolites. Neurochem Res 30, 129–137 (2005). https://doi.org/10.1007/s11064-004-9694-3
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DOI: https://doi.org/10.1007/s11064-004-9694-3