Abstract
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor superfamily known to regulate adipocyte differentiation. However, its role in skeletal muscle differentiation is not known. To investigate possible involvement of PPARγ in skeletal muscle differentiation, we modulated its expression in C2C12 mouse skeletal muscle cells by stable transfection with sense or antisense plasmid constructs of PPARγ cDNA. Phenotypic observations and biochemical analysis of different myogenic markers showed that altered expression of PPARγ inhibited the formation of myotubes, as well as expression of muscle-specific myogenic proteins including myogenin, MyoD and creatine kinase activity. Together, we show that critical expression of PPARγ is required for skeletal muscle cells differentiation.
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Acknowledgements
We thank Dr. C.L. Kaul, Ex-Director, NIPER, for his support in this work. Authors greatly acknowledge Dr. R.M. Evans, The Salk Institute for Biological Studies, San Diego, CA, USA for the generous gift of pCMX-mPPARg plasmid. We are thankful to Dr. N. Kumar and Dr. A. Khurana for his constant support. R. Singh for his assistance in the laboratory. J.K.S., B.K. and K.S.M. are recipients of NIPER fellowship and N.K.V. was research scientist of NIPER.
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*These authors contributed equally to this work.
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Singh, J., Verma, N.K., Kansagra, S. et al. Altered PPARγ expression inhibits myogenic differentiation in C2C12 skeletal muscle cells. Mol Cell Biochem 294, 163–171 (2007). https://doi.org/10.1007/s11010-006-9256-x
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DOI: https://doi.org/10.1007/s11010-006-9256-x