Abstract
The V-ATPases are responsible for acidification of intracellular compartments and proton transport across the plasma membrane. They play an important role in both normal processes, such as membrane traffic, protein degradation, urinary acidification, and bone resorption, as well as various disease processes, such as viral infection, toxin killing, osteoporosis, and tumor metastasis. V-ATPases contain a peripheral domain (V1) that carries out ATP hydrolysis and an integral domain (V0) responsible for proton transport. V-ATPases operate by a rotary mechanism involving both a central rotary stalk and a peripheral stalk that serves as a stator. Cysteine-mediated cross-linking has been used to localize subunits within the V-ATPase complex and to investigate the helical interactions between subunits within the integral V0 domain. An essential property of the V-ATPases is the ability to regulate their activity in vivo. An important mechanism of regulating V-ATPase activity is reversible dissociation of the complex into its component V1 and V0 domains. The dependence of reversible dissociation on subunit isoforms and cellular environment has been investigated.
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Abrami L, Lindsay M, Parton RG, Leppla SH, van der Goot FG (2004) J Cell Biol 166:645–651
Arai H, Terres G, Pink S, Forgac M (1988) J Biol Chem 263:8796–8802
Arata Y, Baleja JD, Forgac M (2002a) J Biol Chem 277:3357–3363
Arata Y, Baleja JD, Forgac M (2002b) Biochemistry 41:11301–11307
Beyenbach KW, Wieczorek H (2006) J Exp Biol 209:577–589
Flannery AR, Graham LA, Stevens TH (2004) J Biol Chem 279:39856–39862
Forgac M (2007) Nat Rev Mol Cell Biol 8:917–929
Geyer M, Yu H, Mandic R, Linnemann T, Zheng YH, Fackler OT, Peterlin BM (2002) J Biol Chem 277:28521–2859
Hirata R, Graham LA, Takatsuki A, Stevens TH, Anraku Y (1997) J Biol Chem 272:4795–4803
Hirata T, Iwamoto-Kihara A, Sun-Wada GH, Okajima T, Wada Y, Futai M (2003) J Biol Chem 278:23714–23719
Hurtado-Lorenzo A, Skinner M, El Annan J, Futai M, Sun-Wada GH, Bourgoin S, Casanova J, Wildeman A, Bechoua S, Ausiello DA, Brown D, Marshansky V (2006) Nat Cell Biol 8:124–136
Imamura H, Nakano M, Noji H, Muneyuki E, Ohkuma S, Yoshida M, Yokoyama K (2003) Proc Natl Acad Sci U S A 100:2312–2315
Inoue T, Forgac M (2005) J Biol Chem 280:27896–27903
Iwata M, Imamura H, Stambouli E, Ikeda C, Tamakoshi M, Nagata K, Makyio H, Hankamer B, Barber J, Yoshida M, Yokoyama K, Iwata S (2004) Proc Natl Acad Sci 101:59–64
Kane PM (2006) Microbiol Mol Biol Rev 70:177–191
Kawasaki-Nishi S, Nishi T, Forgac M (2001a) Proc Natl Acad Sci 98:12397–12402
Kawasaki-Nishi S, Nishi T, Forgac M (2001b) J Biol Chem 276:17941–17948
Kawasaki-Nishi S, Bowers K, Nishi T, Forgac M, Stevens TH (2001c) J Biol Chem 276:47411–47420
Kawasaki-Nishi S, Nishi T, Forgac M (2003) J Biol Chem 278:41908–41913
Leng XH, Nishi T, Forgac M (1999) J Biol Chem 274:14655–14661
Manolson MF, Wu B, Proteau D, Taillon BE, Roberts BT, Hoyt MA, Jones EW (1994) J Biol Chem 269:14064–14074
Murata T, Yamamoto I, Kakinuma Y, Leslie AG, Walker JE (2005) Science 308:654–659
Parra KJ, Kane PM (1998) Mol Cell Biol 18:7064–7074
Parra KJ, Keenan KL, Kane PM (2000) J Biol Chem 275:21761–21767
Pietrement C, Sun-Wada GH, Silva ND, McKee M, Marshansky V, Brown D, Futai M, Breton S (2006) Biol Reprod 74:185–194
Qi J, Forgac M (2007) J Biol Chem 282:24743–24751
Sennoune SR, Bakunts K, Martinez GM, Chua-Tuan JL, Kebir Y, Attaya MN, Martinez-Zaguilan R (2004) Am J Physiol 286:C1443–C1452
Seol JH, Shevchenko A, Shevchenko A, Deshaies RJ (2001) Nat Cell Biol 3:384–391
Shao E, Forgac M (2004) J Biol Chem 279:48663–48670
Shao E, Nishi T, Kawasaki-Nishi S, Forgac M (2003) J Biol Chem 278:12985–12991
Smardon AM, Tarsio M, Kane PM (2002) J Biol Chem 277:13831–13839
Sun-Wada GH, Toyomura T, Murata Y, Yamamoto A, Futai M, Wada Y (2006) J Cell Sci 119:4531–4540
Toyomura T, Murata Y, Yamamoto A, Oka T, Sun-Wada GH, Wada Y, Futai M (2003) J Biol Chem 278:22023–22030
Trombetta ES, Ebersold M, Garrett W, Pypaert M, Mellman I (2003) Science 299:1400–1403
Wagner CA, Finberg KE, Breton S, Marshansky V, Brown D, Geibel JP (2004) Physiol Rev 84:1263–1314
Wang Y, Cipriano D, Forgac M (2007) J Biol Chem 282 (in press)
Wang Y, Inoue T, Forgac M (2004) J Biol Chem 279:44628–44638
Wilkens S, Inoue T, Forgac M (2004) J Biol Chem 279:41942–41949
Xu T, Forgac M (2001) J Biol Chem 276:24855–24861
Zhang Z, Inoue T, Forgac M, Wilkens S (2006) FEBS Lett 580:2006–2010
Zhong X, Malhotra R, Guidotti G (2000) J Biol Chem 275:35592–35599
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Qi and Wang contributed equally to this work.
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Qi, J., Wang, Y. & Forgac, M. The vacuolar (H+)-ATPase: subunit arrangement and in vivo regulation. J Bioenerg Biomembr 39, 423–426 (2007). https://doi.org/10.1007/s10863-007-9116-8
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DOI: https://doi.org/10.1007/s10863-007-9116-8