Abstract
The eleven most known landraces from central and southern Italy were analysed using ISSR markers on 15 randomly chosen individuals for each landrace, with the aim of assessing genetic variation within and among landraces and possibly ascertaining their origin and genetic relationships. A total of 164 loci were observed, 128 of which (78.05%) were polymorphic. Gene diversity over all landraces was I = 0.3759. The highest within-landrace diversity was observed in samples from the Apennine ridge and for one Sicilian landrace; on the other hand, samples from the small Sicily islands were less variable. Principal Component Analysis and AMOVA allowed the discrimination of groups of landraces with higher similarity. Analyses indicate that the small Sicily islands landraces are very closely related to one another and seem to be derived from the peninsular material; moreover, they help disclose relationships among geographically close landraces. The knowledge so acquired can, therefore, contribute to elaborate strategies for increasing the economical value of élite landraces and to protect producers from frauds.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abo-elwafa A, Murai K, Shimada T (1995) Intra-and inter-specific variations in Lens revealed by RAPD markers. Theor Appl Genet 90:335–340
Barulina HI (1930) Lentils of the USSR, other countries. Bull Appl Bot Plant Breed (Leningrad) 40:1–319
Beebe S, Rengifo J, Gaitan E, Duque MC, Tohme J (2001) Diversity and origin of Andean landraces of common bean. Crop Sci 41:854–862
Bejiga G, Tsegaye S, Tullu A, Erskine W (1996) Evaluation of Ethiopian landraces of lentil. Genet Resour Crop Evol 43:293–301
Doebley J, Stec A, Hubbard L (1997) The evolution of apical dominance in maize. Nature 386:485–488
Erskine W (1997) Lessons for breeders from landraces of lentil. Euphytica 93:107–112
Erskine W, Muehlbauer FJ (1991) Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. Theor Appl Genet 83:119–125
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1: 47–50
Gambino R, Romano B (2003) Territorial strategies and environmental continuity in mountain systems: the case of the Apennines (Italy). Proc. World Heritage Mountain Protected Area Field Workshop, IUCN 5th World Parks Congress, Durban, South Africa, pp 2–16
Grandillo S, Ku HM, Tanksley SD (1999) Identifying the loci responsible for natural variation in fruit size and shape in tomato. Theor Appl Genet 99:978–987
Hammer K, Knüpffer H, Laghetti G, Perrino P (1992) Seeds from the past. A catalogue of crop germplasm in South Italy and Sicily. Germplasm Institute of CNR (ed), Bari, Italy
Hammer K, Knüpffer H, Laghetti G, Perrino P (1999) Seeds from the past. A catalogue of crop germplasm in north-central Italy. Germplasm Institute of CNR (ed), Bari, Italy
Harlan JR (1992) Crops and Man. American Soc Agron Crop Science Madison, WI, USA
Havey MJ, Muehlbauer FJ (1989) Variability for restriction fragment lengths and phylogenies in lentil. Theor Appl Genet 77:839–843
Iruela M, Rubio J, Cubero JI, Gil J, Millán T (2002) Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD, ISSR markers. Theor Appl Genet 104:643–651
ISTAT. (2000) Annuari Statistici dell’Agricoltura Italiana. Roma, Italy, in Italian
Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270
Laghetti G, Perrino P, Olita G, Hammer K (2001) Multicrop collecting expeditions in Aeolian archipelago (Italy). Plant Genet Resour Newslett 128:26–34
Laghetti G, Volpe N, Sonnante Ga, Pignone D, Sonnante Gi (2005) Salvaguardia, caratterizazione e valorizzazione dell’antico agro-ecotipo pugliese “Lenticchia di Altamura”. VII National Congress Biodiver., Catania, Italy, p 148, in Italian
Làzaro A, Ruiz M, de la Rosa L, Martìn I (2001) Relationships between agro/morphological characters and climatic parameters in Spanish landraces of lentil (Lens culinaris Medik). Genet Resour Crop Evol 48:239–249
Negri V (2003) Landraces in central Italy: where and why they are conserved and perspectives for their on-farm conservation. Genet Resour Crop Evol 50:871–885
Negri V, Becker H, Onnela J, Sartori A, Strajeru S, Lalibert B (2000) A first inventory of on-farm conservation and management activities in Europe including examples of formal and informal sector cooperation. In: Laliberté B, Maggioni L, Maxted N, Negri V (eds) In situ and On-farm Conservation Network, IPGRI, Rome, Italy
Nei M (1972) Genetic distance between populations. Am Nat 106:282–283
Newman PB (2001) Daily life in the middle ages. McFarland & Company, Jefferson, NC, USA
Piccioni L (1993) La grande pastorizia transumante abruzzese tra mito e realtà. In: Costantini M, Felice C (eds) Abruzzo e Molise. Ambienti e civiltà nella storia del territorio, Mantova, Italy, pp 195–230, in Italian
Piergiovanni AR (2000) The evolution of lentil (Lens culinaris Medik.) cultivation in Italy and its effects on the survival of autochthonous populations. Genet Resour Crop Evol 47:305–314
Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112
Racheli G (1987) Le isole Ponziane rose dei venti. Natura, storia, arte. Mursia (ed) Milan, Italy, in Italian
Raina SN, Rani V, Kojima T, Ogihara Y, Singh KP, Devarumath RM (2001) RAPD, ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44:763–772
Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, NY
Sharma SK, Dawson IK, Waugh R (1995) Relationships among cultivated and wild lentils revealed by RAPD analysis. Theor Appl Genet 91:647–654
Sharma SK, Knox MR, Ellis THN (1996) AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theor Appl Genet 93:751–758
Sonnante G, Pignone D (2001) Assessment of genetic variation in a collection of lentil using molecular tools. Euphytica 120:301–307
Sonnante G, Stockton T, Nodari RO, Becerra Velasquez VL, Gepts P (1994) Evolution of genetic diversity during the domestication of common-bean (Phaseolus vulgaris L.) Theor Appl Genet 89:629–635
Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sonnante, G., Pignone, D. The major Italian landraces of lentil (Lens culinaris Medik.): Their molecular diversity and possible origin. Genet Resour Crop Evol 54, 1023–1031 (2007). https://doi.org/10.1007/s10722-006-9153-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-006-9153-x